FastQCFastQC Report
Sat 31 Dec 2022
SRR5848581.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848581.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91236
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC10411.1409969748783375No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT8110.8889035030032004Illumina PCR Primer Index 12 (95% over 21bp)
CTGCACGGGATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGT2300.252093471875137No Hit
CAAAGGGGGCAAAGAACAAGTCATTAGAGAAATTAAAAATTATTTTGAACTA1930.21153930466044105No Hit
TCAATGGGGATTGGAAAGCCAGAAGTTGAATATGATTGTGATAATTTGCAAC1920.2104432460870709No Hit
ACTGCAGGGATGCGTACCAGGGGATGGATTTAAGCATTTGTGTGTAATAGGA1820.19948266035336928No Hit
TTTAAAAGGGAGGATTCGAGAAGATATTGCAAAATATTTGAGGAATTTAGAT1800.19729054320662895No Hit
GGTAGCTGGGGTTTTTACAATGGATTCTTAGATATGACACCAAAGTACAAGC1770.19400236748651847No Hit
GAATTAGGGTGAGTGTCTTAATATTTCTCAAAATTAATTTTTATATATTTAT1660.1819457231794467No Hit
GTTAATGGGATATTTAAACGGCTGAAGGAAAAAAACTTTTACCCAGGAATAG1640.17975360603270638No Hit
CGCACAGGGACTGTGTCTGACTCTCAACAAATAATGACAAGAAATGAAAGGC1580.17317725459248542No Hit
CGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1580.17317725459248542No Hit
ATACCGGGGAGCTGATTAGTAGGTGAGATCATGAAAGAGTTGACTGCAGAGG1570.17208119601911526No Hit
TAACTTGGGATGGTGGTGCAGTTCTTACGAAACTTTGGTAAAGTTTTGGGCT1550.16988907887237495No Hit
CAACCAGGGACCTATTAGAAATGCACTTTACCTGTAAGGACACAAGTAAACT1510.1655048445788943No Hit
CAATGCGGGTGTGTGGCTCAGAGGTGAAGCGTTGGAAGGGTTTGTGGGAGAT1510.1655048445788943No Hit
AAATATGGGAGGATCAAATCTTAATATAACTATAATCAGTTTTCTCTTTATA1510.1655048445788943No Hit
GGTATGGGGGGGCGGGGGCCGGCAGGTGCTCCGCAGCCGTCTGTGCCACCCA1490.16331272743215397No Hit
TGTGGCGGCATTAAGTATAAAATAATTAAACATTCAAATCAAAAGGGAGAAA1470.16112061028541363No Hit
GCGTTCGGGTGTTTGCAGACAACATGATTCTATATTTAGAAAACACCATCAT1470.16112061028541363No Hit
GCTTTACGGGGTAGTGAAACATCAAGTCCAATTGCAGGCAACTCTCCCTTTT1410.15454425884519268No Hit
GGAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1410.15454425884519268No Hit
TACGACAGGGTCTTCCGATCTTGCTTGGGGAGCAGAAAGAAGAAAATAACAA1390.15235214169845238No Hit
GAAGCGAGGATATTTAAGACTTATTGTAGGAAGTAATTTTAAATCTATTTCA1350.1479679074049717No Hit
GTTTAGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTT1350.1479679074049717No Hit
AGTGCTGGGTATTTGATGTGTTGCCTGGCAGGATACTGCAAAGAACATGGTT1350.1479679074049717No Hit
AACTATGGGGGCTAATGAGTCATTGTTCCTTAATATCGTTTTCAATATGCCA1320.14467973168486123No Hit
AAATGCGGGATTCCTAAGTTCTGCTTTCTGGAGAGGTCGTGAGTGGGCAGAG1320.14467973168486123No Hit
CGTCACGGGTCTACTCAACAACTGCAGGATATACATTACTTTCTTCAACACA1310.1435836731114911No Hit
GTAATAGGGCACAACGTGCATTGTGCTTCTTTCTTTGGGAATATTTGAATCT1280.1402954973913806No Hit
ACGGGTGGGCATGACAACTGAAATATCTTTCAAGGAAGTGATAGAAGCAATT1270.13919943881801042No Hit
CGCAATGGGGATCCAAAGAGCCCTTCGAGCACCACGCAAGAAGATCCATCGC1270.13919943881801042No Hit
TGTTGCGGGATGTGAAAAATGTAATTTACTAAAACTGACACATGAAGAAATA1270.13919943881801042No Hit
GCGCATGGGACTGTTGGTGGGAAAGTGAAATGGTATAGCCACTGTGGACAAT1270.13919943881801042No Hit
CAGTATGGGGAAAGGAAAGAGGTCGGGAGCGCTCGCGAGATCTCGGACCACC1240.13591126309789994No Hit
TGAATAGGGAAATTATCCAGTCTTAGGTAGTTCTTTATAGCAGTGTGAGAAT1230.1348152045245298No Hit
CTCTGAGGGATTCCCGGGCTCGATGCCGGAAAAACACCTTGGCCCGCCGGAA1220.13371914595115963No Hit
AACATGGAGGACAAAGTAGGTGTGTCACGGAAGAAAGTGCTTAGGGAAGATT1210.13262308737778947No Hit
TATGTAGGGAGCTATGACTATCTCCAAAATGCACCTCCTGGATTTTTTCCGA1210.13262308737778947No Hit
TTGACTCGGGCGGTCGAGTCACCCCCAACCTCATGGGCCACCTCTGTGGAAA1210.13262308737778947No Hit
ATCAACGGGATGGTCACCAGCCCAAGGCTGCCTCATGCTCTTCGTGACTTAC1190.13043097023104916No Hit
TAACTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1190.13043097023104916No Hit
TATAGGGGACACATCAACAGGAAGGTAAGACAGCATTGCTGGGCCAAAAGTC1190.13043097023104916No Hit
CTCAAAGGGAGGATGAGTTCAACAATGACTTAATAGCATCAGAATTTTGACA1180.129334911657679No Hit
ACCATGGGGGATCTCATAGAGGGAAGGATGTTGTGGAACAAATGGAAAAATG1180.129334911657679No Hit
GCAAAAGGGCTCCCAGGTGTTGTCCAAGACCATCAAGGTAGTACGTCTACGA1170.12823885308430882No Hit
TCTTTAGGGACTGGCCTCCCTTGCAGGCCACCTCTCTTTCTTTCTTGGAAGA1170.12823885308430882No Hit
TGGTTCGGGGGAAAAAGCCTTTTAGATGAAAGTCAATCTTCGGTTTTGTTGT1150.1260467359375685No Hit
GCTCCGGGGAAAAAGCATCTTCCATGAAACCAGTCCCTGGTGGCAAAAGGGC1140.12495067736419835No Hit
TGTAATGGGATTAGAAAGGCTCAATAGTAGATTTGAACTGGGAGAAGAAACA1140.12495067736419835No Hit
AAGCCAGGGAGTGGGAACAGGCCCCAGCTCAGCCTCCGGCAGGGAGGTCACC1130.1238546187908282No Hit
AATCTGGGACTATTTATTGAGTGCCTATTATGTGCCTGGCTCTGTGCTAGGC1110.12166250164408786No Hit
AAACTTGGGGCTGTCCAATAGAACTTTCTGTGATGATGAAAACGTGTAAAGT1110.12166250164408786No Hit
AATGTGGGCAGCCACGACTCCTCCTTCCTCTGCGCTTCCAGTGATAAGGGTA1100.12056644307071769No Hit
GGTGCCGGGGGATACTTCTAATAAATGGAATTTCATTTTATAGACTGTATAT1090.11947038449734754No Hit
GGTCACGGGACCATATGTTTCTTTTTTGTGTAGATATTAATAACATTACTCT1090.11947038449734754No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTC1080.11837432592397738No Hit
TTACTAGGGATCTGTGATTGATAAATATTTGTTTCATGAAGGAACCTGTGAG1080.11837432592397738No Hit
AAGCACGGGCATAGAGTGGTCTTTTGAACTGGTATCTAAACATACCTGGTTA1070.11727826735060722No Hit
TATCAGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTT1040.11399009163049674No Hit
GCTTTGTGGGAAGACCATGTTGTAGAAGAATCTTTAATGTTGTTGGAAAAAT1040.11399009163049674No Hit
TGCCGGGGCAGGTTTATACAGAGATGTCTAGACTGCTTCCTCCTGATTAGAA1040.11399009163049674No Hit
TCTATAGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT1030.11289403305712659No Hit
CCTTTAGGGTGGTGAGGTCAACGTTGTCACATACCTTCACATAAGGGATAGT1020.1117979744837564No Hit
CTACTCATGGGGTGTATATATAGATGTATGTAAATATGTACATATATTTATG1010.11070191591038625No Hit
ATCTGAGGGATTGGCTGTCATGAATATTACTGGTCTTCCGGACTTCCAAAGG1010.11070191591038625No Hit
AACTTAGGGAGAGGTGAAATACAGAAAGCCTTTTTTTCTTGATCTTTTCCCG1010.11070191591038625No Hit
CGAGTGGGCAACTGTAAGGAAACATAGGGGAGCCACATGACCAAGCAAGAGT1010.11070191591038625No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCACTGTCTCTTATACAC980.10741374019027577No Hit
GGCGTTGGGACTGTTCTGCATCTTTCTTTTTCACTTAACCTTGGACGTCTTT980.10741374019027577No Hit
ATAGAAGGGAAGGAAGGAGTAGGTCGGGAAGTTCTAGTAGTGGTAGTTCTAG970.10631768161690561No Hit
GGGCTAGGGGGACTGCAATAAATGCGGTGGAGGCATCAGAACTATTTTGGCA970.10631768161690561No Hit
GACTTAGGGACTTAAATGGAGAGATGGCATTGGCCACTGTATTAGTCTGTGT960.10522162304353545No Hit
AGAAATGGGCTACCACACCTGACTTGTTCCTTATTTTAGATGTTGAAAATAA960.10522162304353545No Hit
AACGCAGGGATCCCACCTACAAGTATGACACCTGTGTAACACCAGCTAGGTA960.10522162304353545No Hit
CTCTCAGGGACATACTCATAAGACAGAATTAAATCAGACAATACAAGAACTG960.10522162304353545No Hit
GTCAGAGGGCGATCTCAGTAAGGGCGATCTAAGCTTGGGCTTGGCCAGGAAC960.10522162304353545No Hit
ATGGATGGGACACTCAGAGGAAACCCATTCAGTGGAAATTATACTATGAAAT960.10522162304353545No Hit
TTCAGTGGGATCAGAGTATATTTGGGCTGATCGTCTCACTAAATACCCTTTG950.10412556447016527No Hit
TCAACAGGGAGAGGTGGCAATCCTAATTTCAGACAAAACACACTTTGAACCA950.10412556447016527No Hit
TTGAAAGGGAAGGACAAAGGCGGCAAGGCCAAGAAGACGGCGGCCGCCGGGG950.10412556447016527No Hit
TCATTAGGGTCTGCAAGAGGGCGGCCCAGAAGGTTATATTTAAAAAGAAAGT950.10412556447016527No Hit
TAATGAGGGATGACTTACAGTGACTGTTCTTAAAATGCTTGTGCTTTTTGTT940.10302950589679513No Hit
CATACGGGGAGAGATCACAGTGGAGCACTTTGAAAGACCGTCGGTTGTGTGC940.10302950589679513No Hit
CGTCCCGGGATTGCTGATAGGATTATATTTAATGTGAATAATTTTTAAAATT930.10193344732342498No Hit
AGGCCGGGTGCCCTAACCCTCAAAGGAAAGGAGTCATCTTCTAGAAAGCAAC930.10193344732342498No Hit
CGCCATGGGGGTTCTGGGATACATGTGCAGAACGTGCAGGTTTGTTGCATAG930.10193344732342498No Hit
GGTGTGGGACCAGGAGCCTCCTGATCTGCCAGTCCCTGAGCTCCCAGATTTC930.10193344732342498No Hit
GCTGCCGGGCTCTTCCTCGGCGCTGCCTACGGAGGTGGCAGCCATCTCCTTC920.1008373887500548No Hit
TATACTGGGGCTTTTTTCCGGCGTTCAAGATGTCGAAGCGAGGACGTGGTGG920.1008373887500548No Hit
TCTGCTGGGAGTGTGCTCACTCCTGTGTGATACAGCAGAAGCTGGTCATAGT920.1008373887500548No Hit
ACTAAGACAGGGAGGCCACACCTGGTTACATGAAATGATTTAATCTAGCTTC920.1008373887500548No Hit
CTCGTGGGATGGTACATTTCTGTAACTTCTTTTTAATTTTGCTGTGAACCTA920.1008373887500548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)