FastQCFastQC Report
Sat 31 Dec 2022
SRR5848576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185868
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC15280.822088794198033No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT14010.7537607334237201Illumina PCR Primer Index 12 (95% over 21bp)
ACACGTGGGGGCGTCTTGTTCTTGCCTGGTGTCGGTGGTTAGTTTCTGCGAC2530.1361181053220565No Hit
TTCAACGGGACCAATTAAAAGACACAGACCAGCAAATTGGATATAAAGAGTC2320.12481976456409924No Hit
ACTATGGGGCTGGTAAACTTTTAAAAAGTATCCTTGCCTGGGCTTTACCCCA2150.11567348871241957No Hit
TGACAGGGGAGCTGTTATTACAGAAGAGCCAAAAAATTTTTAAAAAGTTTAT2140.11513547248585018No Hit
AAAACTGGGTCTGGCGAGCGTTTATATTATTTTATAGAACTCAGTGGTCACT2070.11136935889986442No Hit
AGATAGGGGAATCTTTAAAGCCAAAGGTTTTTTTAGACTTGAAAGAAAGAGC2000.10760324531387867No Hit
TGCCATCGGGTTACATCTCTGGATTCATATCTAGCATCTGACATCTTCATTT1980.10652721286073988No Hit
ACAAGTGGGCGTGAACATGGGCAGTAAGGTAGGCAAGACCATTGGAAGTCAC1980.10652721286073988No Hit
GTTAACGGGTGGGAAGAGAGATGGAGTCACATGGATTTTTGGTGGGCATTAT1970.10598919663417049No Hit
GCTCCTGGGATGTCTTAGATTTTGCTTCAGTCTATATGGATATACTTCATTC1940.1043751479544623No Hit
GCGCACAGGAAAGACAAAAAGCAGATTGGTGGTCACTTAGGGCTAGGGTGAG1930.10383713172789293No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)