FastQCFastQC Report
Sat 31 Dec 2022
SRR5848575.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848575.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184431
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC14550.7889129267856272No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT13760.7460784792144488Illumina PCR Primer Index 12 (95% over 21bp)
TGCCATCGGGTTACATCTCTGGATTCATATCTAGCATCTGACATCTTCATTT2180.11820138696856819No Hit
TGACAGGGGAGCTGTTATTACAGAAGAGCCAAAAAATTTTTAAAAAGTTTAT2090.11332151319463647No Hit
TTCAACGGGACCAATTAAAAGACACAGACCAGCAAATTGGATATAAAGAGTC2090.11332151319463647No Hit
ACTATGGGGCTGGTAAACTTTTAAAAAGTATCCTTGCCTGGGCTTTACCCCA2080.11277930499753297No Hit
AAAACTGGGTCTGGCGAGCGTTTATATTATTTTATAGAACTCAGTGGTCACT2050.11115268040622238No Hit
TGCAACGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT2040.11061047220911886No Hit
ACACGTGGGGGCGTCTTGTTCTTGCCTGGTGTCGGTGGTTAGTTTCTGCGAC2020.10952605581491182No Hit
ATTTGCGGGATATTCACCAAAATTCCTGTCTAGCACTCTTCAAAATTGTCAA1910.10356176564677304No Hit
AACGTGGGGAAGACAGAAACTGTTACCTATGAAGAAAAGGAACAACAGCTTG1900.10301955744966952No Hit
ACAAGTGGGCGTGAACATGGGCAGTAAGGTAGGCAAGACCATTGGAAGTCAC1900.10301955744966952No Hit
TATAGAGGGATATGATGATATGTCTGCCCTAAAATAGCTTAGAGCCTGGGGA1880.10193514105546247No Hit
GTTAACGGGTGGGAAGAGAGATGGAGTCACATGGATTTTTGGTGGGCATTAT1860.10085072466125543No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)