FastQCFastQC Report
Sat 31 Dec 2022
SRR5848574.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848574.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180704
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC14830.8206791216575172No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT13060.722728882592527Illumina PCR Primer Index 12 (95% over 21bp)
TGCCATCGGGTTACATCTCTGGATTCATATCTAGCATCTGACATCTTCATTT2370.13115370993447847No Hit
ACTATGGGGCTGGTAAACTTTTAAAAAGTATCCTTGCCTGGGCTTTACCCCA2220.12285284221710643No Hit
TGACAGGGGAGCTGTTATTACAGAAGAGCCAAAAAATTTTTAAAAAGTTTAT2080.11510536568089251No Hit
AAAACTGGGTCTGGCGAGCGTTTATATTATTTTATAGAACTCAGTGGTCACT2040.11289180095625996No Hit
GTGCGAGGGGGGCTCCGAAGCGGGGAAGTGGGACAAGATGGTTTACATCTCG2020.11178501859394369No Hit
ACACGTGGGGGCGTCTTGTTCTTGCCTGGTGTCGGTGGTTAGTTTCTGCGAC2020.11178501859394369No Hit
TTCAACGGGACCAATTAAAAGACACAGACCAGCAAATTGGATATAAAGAGTC1990.11012484505046928No Hit
ATAACGGGTTGTGAACAAAGTTGAGGACAGTTGCACTTTCTATCTACTTCTT1980.10957145386931114No Hit
GCTCCTGGGATGTCTTAGATTTTGCTTCAGTCTATATGGATATACTTCATTC1980.10957145386931114No Hit
TGTGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1890.1045909332388879No Hit
GCCACCGGGGTTGTTTTATACATTTAGAATACAGTTAGATTGTTTTGTCAAA1880.10403754205772976No Hit
CACAGAGGGGGTGGTATCAAATTGTTGGACTCTGAAAAACAAGTGGATGGAT1870.10348415087657163No Hit
GATGTTGGGCAATAAACCTGTTGTGAATTGAAAATATCATTAGTCAAAAATG1830.10127058615193908No Hit
GTAACAGGGACTGTGGACAAGAGGTTTGGCATGGATTTTAAAGAAATAGAAT1830.10127058615193908No Hit
CGCATTGGGTACTCAAATGTATGTACACATAGTATTTTGCAGATGATTTCAG1820.10071719497078094No Hit
TGATATGGGAGGCAAGTTGCTTTGGCAGATATCATTCTCATTAATAAAACAG1820.10071719497078094No Hit
GTTAACGGGTGGGAAGAGAGATGGAGTCACATGGATTTTTGGTGGGCATTAT1810.10016380378962281No Hit
TAACGAGGGACCCCAGAGCATTTTAGATGATGTTGCCATGGTACTTGATGAA1810.10016380378962281No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)