FastQCFastQC Report
Sat 31 Dec 2022
SRR5848572.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848572.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232031
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT17290.7451590520232211Illumina PCR Primer Index 12 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC16840.7257650917334322No Hit
GCCAGAAACTGTTCATTTTCTAGTAGGGGTAAGTAAGGTAAGCCATGATTTT3540.15256582094633908No Hit
CGAACAGGGAGCCAAGAGGAGGAGGAAGTCGATGATTGCTAAGCGTCGCCGA3390.14610116751640945No Hit
TAGCTGGGAGGCATAAATGCAAACTAATCACATTAGGGTAAATGGGTATCTA3210.1383435834004939No Hit
AAGTTGGGGGCAGCATTAATATTTAGTCAATATTATTCATTCTTAACCATAT2960.12756916101727786No Hit
CCAGCTGGACACGTTGAAAGCACGCTTGACCCAGAAATACCAGGAACTCCAG2900.12498329964530602No Hit
AATAGCGGGATGAATAGACAATTCTCAAGAAAGGATAAACAACCAACAAACA2670.11507083105274728No Hit
TCGTAAGGGGAGTCCCACAGGTCTGCCATTCCCATTAGGGTCTTTGACCTTG2660.11463985415741862No Hit
TCTGTGGGGTTGCAGCAGTGGGAGCAATTCATCTGGAGGAGAGTGAATCGCT2630.1133469234714327No Hit
ACATCTGGGAATCTGTATGGTATCTGAAAGATACTATAGCAGTGGTCTGTGA2630.1133469234714327No Hit
CCAACCGGGGGATTTTGACCACCTTTGAGACTTTCTGATAGTATTTAAATTT2590.11162301589011812No Hit
TTCTATGGGATACCAGGCACGGATTGTGAGCCATGCCAATACGGGCGTTTTG2540.10946813141347492No Hit
CGTAACGGGTGGTTGATTTTGAGAATATCTAAACTTTTTCATAGAGACCAAA2520.10860617762281766No Hit
CATACAGGGAACACGAGGCAATATTGTAGTCCACAGGGAATGGATGCTTCAC2480.10688227004150308No Hit
ACATTTGGGTTGGAAACAAAGGGGGAAAGCTGGTTTATTCCATGTAGGTTGC2450.10558933935551715No Hit
CGCATCGGGACAGCACGGGGACCCTGGGCTCAGCCCACAAGACCATTTTTCC2400.10343445487887394No Hit
GCGTATGGGTCCGATCTTCCAGGGGGATTAAGTGTAATTTGCCACATATAGC2360.10171054729755938No Hit
AAGAGTGGGAGGAATAAAAGGGAATTTCCCAACTTGATAAAGAGTGCCTACA2350.10127957040223073No Hit
GCCCGTATGAACAGAGTTGGTGGAGGCAGCAACTTCACAGGTGAATCAAAGA2350.10127957040223073No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)