FastQCFastQC Report
Sat 31 Dec 2022
SRR5848571.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848571.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229627
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT16960.7385891031977947Illumina PCR Primer Index 12 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC16350.7120242828587231No Hit
GCCAGAAACTGTTCATTTTCTAGTAGGGGTAAGTAAGGTAAGCCATGATTTT3250.14153387885570948No Hit
TAGCTGGGAGGCATAAATGCAAACTAATCACATTAGGGTAAATGGGTATCTA3150.1371789902755338No Hit
AAGTTGGGGGCAGCATTAATATTTAGTCAATATTATTCATTCTTAACCATAT3090.1345660571274284No Hit
CGAACAGGGAGCCAAGAGGAGGAGGAAGTCGATGATTGCTAAGCGTCGCCGA3080.13413056826941083No Hit
CCAGCTGGACACGTTGAAAGCACGCTTGACCCAGAAATACCAGGAACTCCAG2550.11104965879447973No Hit
CCAACCGGGGGATTTTGACCACCTTTGAGACTTTCTGATAGTATTTAAATTT2550.11104965879447973No Hit
AGCTTTGGGCATTTAGGTTGAAATTAGGGAATCTAGCATTGTTATTGGTGTT2530.11017868107844461No Hit
AATAGCGGGATGAATAGACAATTCTCAAGAAAGGATAAACAACCAACAAACA2470.1075657479303392No Hit
TAACATGGGAGTCTGATTACTTTGTGAGTCATCAAATTGATTTGACTGTCCT2390.10408183706619865No Hit
GCGTATGGGTCCGATCTTCCAGGGGGATTAAGTGTAATTTGCCACATATAGC2370.10321085935016353No Hit
TCGTAAGGGGAGTCCCACAGGTCTGCCATTCCCATTAGGGTCTTTGACCTTG2350.10233988163412838No Hit
CGTAACGGGTGGTTGATTTTGAGAATATCTAAACTTTTTCATAGAGACCAAA2310.10059792620205812No Hit
TTCTATGGGATACCAGGCACGGATTGTGAGCCATGCCAATACGGGCGTTTTG2310.10059792620205812No Hit
TGATGGGGGTCTTTACAGTTTGGCATGTTTTTGCAGTGGCTGGTACCGGTTG2310.10059792620205812No Hit
GCAAAGTGGGTGTTTATGACTGCTGTTTTGTGTATTCCCGATGAACACATGC2310.10059792620205812No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)