FastQCFastQC Report
Sat 31 Dec 2022
SRR5848565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371326
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC14510.3907617565158378No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT12330.33205323623985394Illumina PCR Primer Index 12 (95% over 21bp)
AATACTGGGACCAAATAAATGTTTATTGGGTCATAATTAAATTGTGAACAGT4170.11230024291323526No Hit
GCTCCTGGGATACACAGGAGGATGATAATCCATCAAAAATATTCAGGCCATC4030.10852997096890601No Hit
GATTGAGGGCATGGCAGAAGGCAAAGGGGCGTCAGCATGTCTCATGCAGTGA3940.1061062247189801No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)