FastQCFastQC Report
Sat 31 Dec 2022
SRR5848557.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848557.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319886
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC13540.4232757920009003No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT13120.41014611455330957Illumina PCR Primer Index 12 (95% over 21bp)
ACTGCAGGGGCCCGCCCCCGAGCAGGAGCCGGTGCGAGCGGAGCAGAGCCGA3370.10535003094852541No Hit
GAAATAGGGCAAGATAGAAATCCATACACATATAAAATGTAATTTCCCATGA3300.10316175137392695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)