FastQCFastQC Report
Sat 31 Dec 2022
SRR5848534.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848534.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183669
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC13380.7284843931202326No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT11470.6244929737734729TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC4140.2254054848667984No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA3820.20798283869351933No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2550.13883671169331788No Hit
ATTGATGGGGTGCAGGAGGAGGGAGAGAAAAGAAGAGGTTTGAGAATAGGAA2100.11433611551214413No Hit
GAAAGGGGTCTCTACACAGTATCGTTTCCTGTTTAAATTACAGATAACTTCA1970.10725816550424948No Hit
TGCACGGAGAGATGGCAAGGCCACAGGTGATCTGAGAACTATTTTTGTCTGA1930.1050803347325896No Hit
TCAATTGGGAATGTCTAAAAACAAGCAACATAATATTGTTAAAAATCATTAA1870.10181358857509978No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)