FastQCFastQC Report
Sat 31 Dec 2022
SRR5848533.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848533.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184107
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC13250.7196901801669682No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT12650.6871004361594073TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC4260.2313871824536818No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA3750.20368590004725515No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2670.14502436083364564No Hit
ATTGATGGGGTGCAGGAGGAGGGAGAGAAAAGAAGAGGTTTGAGAATAGGAA2050.1113482920258328No Hit
GAAAGGGGTCTCTACACAGTATCGTTTCCTGTTTAAATTACAGATAACTTCA1930.10483034322432065No Hit
CTAAAGAGGGAAACATAAATGTAAACAACATTCAGTTTCTTCTTTCCACCTT1880.10211453122369057No Hit
ATCTTAGGGAGTTCCACGATTTGGCTGCTTTTGAGTCTAAATCTGAAGATAT1870.10157136882356457No Hit
TCAATTGGGAATGTCTAAAAACAAGCAACATAATATTGTTAAAAATCATTAA1860.10102820642343856No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)