FastQCFastQC Report
Sat 31 Dec 2022
SRR5848518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147332
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT13030.8843971438655553TruSeq Adapter, Index 11 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC12130.8233106181956398No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC3270.22194770993402657No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA3070.2083729264518231No Hit
ATTTTGGGGATCTTCGTCCGGGGAGACCCATTAAGTACTATTTCAATTACTT1950.1323541389514837No Hit
GCAACAGGGAGAGTGAATCCAGGGGCTCTATTTTGACATGAATTAACTCGCA1840.12488800803627181No Hit
GCAAATGGGAGAAAGAATGAATGGACACATGAACGACTGAATTAGAAATGGA1800.12217305133983114No Hit
AGACACGGGAAGTGTTGTGAAGTTCAGAGTGGCAGATACATATTTCCAAAAT1770.12013683381750062No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC1760.11945809464339044No Hit
TTCACGGGAAAAGAATTAAAGAAAAATGAAATGTAAATATTTAAAATAAACA1760.11945809464339044No Hit
CAGTCAGGGAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCT1750.11877935546928027No Hit
TTTTATGGGGGCTTAAGAGCCAAAGTGTCTAAACAGGATCGTTAATACAGTT1720.11674313794694975No Hit
GCCGGGACATGCAAGACAGAAAGATACAGTATGTGGTTTATAAATGCATATT1670.11334944207639888No Hit
TTCGTGGGAAGATGGCGGGTGAAAAAGTTGAGAAGCCAGATACTAAAGAGAA1650.11199196372817853No Hit
TGATCCGGGTTTAAAGAAATTTAACAAATCAGTGTTTTTCAGGTTTTAAAAA1650.11199196372817853No Hit
AGTGCAGCGGTATAGGTGATTTTGTACTCTCTGCTAAGATAAAATTTTCTCC1640.11131322455406836No Hit
TGTCCGGGCTGGTCTCGAACTCCTGACCTCGGGTGATCTGCCAGCTTCAGCC1630.11063448537995818No Hit
CAAACGGGGAGCAAAGCATATTCTTGAACTTTTAAGAGGAAAAAATACCAAC1620.10995574620584801No Hit
GCAACAGGGAGTGAAAGTAAGAGATTTAAAATAGGTTCTCAACTCCTGATAA1620.10995574620584801No Hit
GTGCCTGGGGACGAACCGAAAAATAATTAATGCAGAGGAGATTTATGTCTTA1610.10927700703173784No Hit
TTGCGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1610.10927700703173784No Hit
AGACCAGGGATTTTATTCACGCTAAAGGAATGGGTATTAAAATTAAGTGCAT1610.10927700703173784No Hit
ACAACTGGACATTGATTCCTTTTACACAAGAGCTGCCTCCCAAAGATAGATA1590.1079195286835175No Hit
CTCACAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1580.10724078950940734No Hit
GCTTTTGGAAGGCAATTTGGTCTGAAGAATGATTTGATCTCAGAAAATGTTG1580.10724078950940734No Hit
TGACAGGGACCCCAGAGAGGCAAAGCCAATGTGTGTGTCCTGGTAACATTGT1570.10656205033529714No Hit
AAGCACGGGATCCTGGAACCTTTAATTAAGAATAGCTACACTCACAAAAATA1570.10656205033529714No Hit
TGCTAAGGGGACGCTCTTCCGATCTTAACTCGGGGCTGCTGGGACGTTAGAA1570.10656205033529714No Hit
TTCTTTTGCAGTATATTATTTTCTAAAAATTGTTACATGCAAATTTAAACCA1560.10588331116118699No Hit
TGCGCAGGGGCGGGTGATTCATGTAGCTGGAAAAGAAACCACAAACATCTTC1560.10588331116118699No Hit
AATGCAGGGTGGAGGTTCTATTTCATTTAAAAATGTATTCATTTCCCTGGCC1560.10588331116118699No Hit
TGCACTGGGCCTCTGAGATATGGTTCAAACCCTATCTGTATTTTATGTTTCT1550.10520457198707679No Hit
ATTCCTGGGGTCATGGAGGATTCAGGAAAAGTCCTAATTGTATGTAACTCTG1550.10520457198707679No Hit
TAGCGGGGAGGTACTCAAGACAATTGACGAGGGAGATGCCGATGAGGTGACG1550.10520457198707679No Hit
GTATATGGGATGTAGGTCAACTTGTGTCATAGGGGTTTTTTGTACAGATGAT1540.10452583281296664No Hit
GTATTTGGGTATAGGTTATCTGGTAAGATTTGTCAGTACTGGAGCTGAGCCA1540.10452583281296664No Hit
AGATATGGGAGCCATTGAATTGAATAAGGCGACAGTCACTATGAAAAGGGTT1530.10384709363885647No Hit
TTTAATGGGGTCATTTACATTAGGTATATCTCCTAATGCTATTCCTCCCCCT1530.10384709363885647No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACA1520.10316835446474629No Hit
ATGCAGGAGAGAGTTTTTGTTAAGGATATATTTGAAGATTGGCTTTTCCATA1520.10316835446474629No Hit
GCAACTGGGATATTAGAATAATCAGAAATGACTTTAAAACCACAATTGTAAA1520.10316835446474629No Hit
ATTAGCGGGAACGTACCTGGATCCTTTGGTGGCATTCGATTATACCTGCAGG1510.10248961529063612No Hit
TGTATAGGGATTCCTGAGGAGGTGTGATGTAACAATGCAAATAGTTGAGTTA1510.10248961529063612No Hit
TTCACAGGGGAATTTGACAAATTTGCCAGTGACGGGGAGTCTTGTCCAGAAA1500.10181087611652595No Hit
TCGGTCTGGGCATTTATGTCGCATGTCAATCCGAAGAGTGATGCCCACCCAA1500.10181087611652595No Hit
TAAAACGGGCATGGGTGTAAATTATTAATATCGTAATTTCCTAAAAGCAATT1500.10181087611652595No Hit
AGTACAGGGAAATATGTCTTATAAAAACTGCTTTGCCAGCTACTGGCAAGAT1500.10181087611652595No Hit
GCCTATGGGAGCCAGTGAAGAAGTAGATTCCTAAATTTTATTGGGGACCATG1490.10113213694241577No Hit
TAAACTGGGACTTGATCGAATTGGCTACTGAAGCTTGTGCATGCATCACGTA1490.10113213694241577No Hit
TAGCAAGGGTAGAAAAGTAGTATTGAAATTTCTAACCCATACCCCTGTGAAA1480.1004533977683056No Hit
TGAACTGGGGAACGAGAGGGGGAGAGATGTAAGAAAAGAAAGGAAAAGTGAA1480.1004533977683056No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)