FastQCFastQC Report
Sat 31 Dec 2022
SRR5848517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147766
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC12570.8506693014631241No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT12520.847285573135904TruSeq Adapter, Index 11 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA3330.22535630659285627No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC2960.2003167169714278No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC1860.12587469377258637No Hit
GCAAATTCTCCCAAGCCAGCTTGTGAAGATGTGAAGGCCAAACCAGTATCCG1850.12519794810714235No Hit
TTCACGGGAAAAGAATTAAAGAAAAATGAAATGTAAATATTTAAAATAAACA1830.12384445677625436No Hit
TAAACTGGGACTTGATCGAATTGGCTACTGAAGCTTGTGCATGCATCACGTA1710.11572350879092619No Hit
GCAACAGGGAGAGTGAATCCAGGGGCTCTATTTTGACATGAATTAACTCGCA1700.11504676312548218No Hit
CTGTTGTTGAAGAAAACAGAGTAAAAACCTTTGTGACCTTCAGTTAGTCAAA1690.11437001746003818No Hit
CTTTACGGGGTAAGACAAAGAGATAAAAGGCAGAAAGATTGGAAAGAAAGAA1690.11437001746003818No Hit
CAGGCTGGGATAATTGTGTATGTAGAACTTTTAGCGTTTTTTATTTTTAATT1660.11233978046370613No Hit
TGTTATGGGCAAACAAGGGCATTACATAATAGTAAAGGGATCAATGTAGCAA1640.1109862891328181No Hit
TCATTGGGGAACTGGCATAGGTGAGGTCAGAGCATATAGAAAGAGCAGGTTG1610.10895605213648606No Hit
TCGACGAGGAATGAAATATTTTAAGGACAGCATTGAAACATTGGAAAATTTG1610.10895605213648606No Hit
AAGCACGGGATCCTGGAACCTTTAATTAAGAATAGCTACACTCACAAAAATA1610.10895605213648606No Hit
GGCGCTGGGGTGCCTTTTCAAACTAATGACTTCATTATCCTGGCAGTATGGG1600.10827930647104204No Hit
AAATGCGGGAAAAAGAGATATGGATGCTTCCTCGCTCAGGAGGCCTGAGCTT1600.10827930647104204No Hit
CAGTCAGGGAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCT1600.10827930647104204No Hit
TGCGCAGGGGCGGGTGATTCATGTAGCTGGAAAAGAAACCACAAACATCTTC1600.10827930647104204No Hit
GCTTTTGGAAGGCAATTTGGTCTGAAGAATGATTTGATCTCAGAAAATGTTG1600.10827930647104204No Hit
CAAACGGGGAGCAAAGCATATTCTTGAACTTTTAAGAGGAAAAAATACCAAC1590.10760256080559803No Hit
GCAAATGGGAGAAAGAATGAATGGACACATGAACGACTGAATTAGAAATGGA1570.10624906947471001No Hit
TCAAGAGGGGTATCGACCAGCCTATTTTTATAGTTTTTGTAGAATTTGTAGC1570.10624906947471001No Hit
CTCAGGGACTATTTTTAGGTTTTATTTGCACAAGACTGAATTAGTTTGACAT1560.10557232380926601No Hit
CTCATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1560.10557232380926601No Hit
TTCACAGGGGAATTTGACAAATTTGCCAGTGACGGGGAGTCTTGTCCAGAAA1560.10557232380926601No Hit
AATGCAGGGTGGAGGTTCTATTTCATTTAAAAATGTATTCATTTCCCTGGCC1560.10557232380926601No Hit
ATTCCTGGGGTCATGGAGGATTCAGGAAAAGTCCTAATTGTATGTAACTCTG1540.10421883247837799No Hit
TGATCCGGGTTTAAAGAAATTTAACAAATCAGTGTTTTTCAGGTTTTAAAAA1540.10421883247837799No Hit
TTGGCTGGGATATCACATACATTCATATATCTCAAGAAATGAAAGCAATGAA1540.10421883247837799No Hit
AGTGCAGCGGTATAGGTGATTTTGTACTCTCTGCTAAGATAAAATTTTCTCC1530.10354208681293398No Hit
TGACAGGGACCCCAGAGAGGCAAAGCCAATGTGTGTGTCCTGGTAACATTGT1520.10286534114748995No Hit
ACAAGCGGGTACCATGGCATACACGTTAAGCATTTTGAAGTAAAAATTACAT1510.10218859548204594No Hit
TTCTCTGGGAGGCTTGGATTGTGCCCATGGTTCTGTTCTGTACTAAAGACAC1510.10218859548204594No Hit
ATTTTGGGGATCTTCGTCCGGGGAGACCCATTAAGTACTATTTCAATTACTT1500.10151184981660193No Hit
GCTTGAGGGACTATTTAGAATATTATGGAATTGTGCCATAGCTTCTTTATTT1490.10083510415115791No Hit
TTTAATGGGGTCATTTACATTAGGTATATCTCCTAATGCTATTCCTCCCCCT1490.10083510415115791No Hit
GTAGCCGGGAGGAGAATAACACAGAAAACAAAAGAAAGAAATCAGATGGTCA1490.10083510415115791No Hit
TAGCAAGGGTAGAAAAGTAGTATTGAAATTTCTAACCCATACCCCTGTGAAA1480.1001583584857139No Hit
TTGCGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1480.1001583584857139No Hit
ACTTGAGGGGGGCCCATGGTGTCCCCATGCCAAAGTTGCCCAAGAGAAGAAT1480.1001583584857139No Hit
TCTTTGGGGCCCTGGGACCTGGAACCACATCACTGATCAAATTGGCATGTTC1480.1001583584857139No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)