FastQCFastQC Report
Sat 31 Dec 2022
SRR5848510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94839
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC13301.4023766593911788No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT12781.3475468952646064TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC3390.35744788536361627No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA2740.2889106802054007No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2120.2235367306698721No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACA1520.1602716182161347No Hit
AGCAGAAGGATGTTTGTATTAGATGAAACAGACTTCAGAGCAAAGAAAATTA1420.14972743280717848No Hit
TACGGTGGGGACCACAAGTGTAAGACTGAATGAAAGAATAAATGCGTGCATT1420.14972743280717848No Hit
AAATAGGGGAGATTCATATTGTATTCCCTGCAGCAACCACTAAAAAATGCAA1360.14340092156180476No Hit
CCTTTAGGGCATTTTGAGAATTGCTGTTGATAAGATCCACTGTTTCTTTTCA1330.14023766593911788No Hit
ACTGCTGGGGCACTAGTTTAAATTCCTACCAACAATATGTGAGCATTCCTGT1310.13812882885732664No Hit
TTGATAGGGAGGTTAAATTAATGCCTTCCTTTTTCCTTTATAATCATTTGTT1300.137074410316431No Hit
TGAGTAGGGAATTGCCTGATGAATAAATGCATGAGAAATACTAGTTTTAAAG1290.13601999177553536No Hit
GCGCGGGGGAGCTGAACAGAGACTATCATCAGCAAATAGAGCTCAGCTTTGT1250.13180231761195288No Hit
CTTTCGGGGGAGATATTAGTATTAAAGTTTTTGGTGGCCTAGAGTGAATTAG1230.12969348053016164No Hit
GAAAATGGGGTGATACACAATAAAACAAGACATGCCTGTAGATTGTTTTTAA1210.1275846434483704No Hit
TTTGGCGGGCAATTCTGAATTTGCTTTTCTTGCCTCTCTTTAGTCACCTGTC1190.12547580636657915No Hit
TACAAGGGGAGGACGGGAGCTTGGAGAACGTTTTCTAGAAAAGGGTTAGCTT1190.12547580636657915No Hit
GAACCAGGGATTTCCTAGCTTGTTGAAATGTTCAATTCTTTGTTGGCTTTCT1180.12442138782568352No Hit
GAACCGGGACGGTCAAATCACCTATTGTACCATATTTTGGTGACCATCCCCA1170.12336696928478791No Hit
TTCTATGGGCTGAAAGAATTTGTGAAGACTGGTACTATGATTTTTTTCCTTA1160.12231255074389227No Hit
TCCGCAGGGAAGAGATTAAAAATTAAAGAAGCTATTTCATTGAATATTATAC1160.12231255074389227No Hit
CAGTCCGGGAAATCACTAAGTTGAAGACATATCTCTGCTTCCATATTTATTG1150.12125813220299667No Hit
CAAACAGGGATGAATGAGAAATTAGTTAACATTCTGCTCCACAAGGTAAGAA1150.12125813220299667No Hit
TAAAGAGGGATTTGTGTCAAATGATTTTTGTATCAAAGCTTTCAGAAAAAGG1130.11914929512120542No Hit
ATAAGCGGGATCTGAACTGGGGATCTATGAGTTTTGAAAAAGTGAATAATCA1130.11914929512120542No Hit
AACCTTGGGTGGAGCTGGAAAGAAAAATTCTTTTTCTGTACCTCATGCCTAT1130.11914929512120542No Hit
CTCACTGGGAATATTTTCTCATTCTTTGTGTATAGAAAATTTTAAAATGGTG1120.11809487658030979No Hit
GTTTACGGGCAATATTTCATTTAAAGAACTGTATTAGTCCATTCTCACACTG1110.11704045803941417No Hit
TTTACAGGGGGACAGCTTCAACACAGGGTCCACAGCGTGGGGCATTTCCCAG1110.11704045803941417No Hit
TCCTAGGGGTGTGTCCTGCAAGCTCTATGACGTTATGCACAGCGTTTACTCT1110.11704045803941417No Hit
GTCAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1100.11598603949851853No Hit
GAGGGGAAAGAACAGCAGGCTATTTCATCACCCCTGGGGGCACCAATTTTCA1100.11598603949851853No Hit
TGGTTGGGAGGAATAAGGAAATTTTAAGTATTGTTTAGGGGCCAAATTTGAA1100.11598603949851853No Hit
AGCCCGGGATGAGCCACCATGCCCAGCCAAAGATGAAAACTTTTAAAAAAGG1090.11493162095762292No Hit
CAGATGGGGACACCTGCCTGCATTTAACATAACTCGATTTTTTTCCCTCCCT1090.11493162095762292No Hit
ACTTAAGGGATTTCATACATACCATCAGAAATATGTATGAAAATGATGTGTC1080.11387720241672729No Hit
ATTAAAGGGCCTTAGCCAGGCAAGCACAGTAATGTGTGTTTTGTTCAGCATT1080.11387720241672729No Hit
AAAACAGGGATCCTGTCTCGCCGTATTGCTGTTGAATATAGTGATTCGGAAG1070.11282278387583168No Hit
TCAGACGGGAAGTGCTTCTATTCAGGTATGTCTTTTTTCAAATAAGAAAAGT1070.11282278387583168No Hit
CATGCTGGGAACATTTGGTGAGGAATTAGCAATTTCTTGCCAAAGAAAATTG1060.11176836533493605No Hit
GAATTCGGGATGATATTCTTCTTTAGCTTCAATTTATATATCTCATTAAATG1060.11176836533493605No Hit
TCGACACGGGATGGCACCTGCATAAAAACCACAGACAGGTAACTTTAGGGAC1060.11176836533493605No Hit
ATTGCTGGGCATACTCCCATAAGAATTTTGACGTATTTATCTGCTCTCAAGT1060.11176836533493605No Hit
ACTTGTGGGCGATCTCAGGCCGGGAGGCGCCCACCACCACGCCTCGCTAATT1060.11176836533493605No Hit
CATGCTGGGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC1050.11071394679404044No Hit
GCTAATGGGAAGTGGAAATGAATTTTTAGCTATTATACAACTTTGGAAAATT1050.11071394679404044No Hit
ACTTATGGGAAATGTAATAATGTATTACAATTAAATGTCTGAATAAAAAAGT1040.1096595282531448No Hit
CGTAGCGGGAGGAGAAAAAGGATGTGCCACACCAACTATATTGATGAAGTCA1040.1096595282531448No Hit
ACGCGGGGAAGTTTCGGTTGAACACCTTTGGAAGCCAAGTCCTCATATTTAA1040.1096595282531448No Hit
CAAGAAGGGATAAGAGTTTATATTAAGGACCAAATTCAGTTCCTTGGTCCTT1040.1096595282531448No Hit
GTGATAGGGAAGTACTCTTTAAGGACACACAGCCCAGGCTGTTCTGAGTCAG1040.1096595282531448No Hit
TCCGCCGGGAAACAACTTCATGTTACTAATGAGAACATGGAAGTGACTAACC1020.10755069117135356No Hit
GTAAAGGGGATTAGAATCTATTATGCATAATATATTTTGTGGGAGAAAATCT1020.10755069117135356No Hit
TTGTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1020.10755069117135356No Hit
CGATTTGGGCTTGGAAAGACGAAAAGCATCCTGGGTTCAGGCTGACATCTGC1020.10755069117135356No Hit
ATGCTAGGGAGAATCTACAATGAACTCCAACAAATGTACAAGAAAAAAACAA1010.10649627263045794No Hit
TATAAAGGGACTGGTGGTGATAAGAGGAGCCGTCTGGTGTGTCAGGGTCACG1010.10649627263045794No Hit
CTCGCCGGGAACTGAATAAATTTGAATTTCTGTAGGAGGTAAAGAATCAAAA1010.10649627263045794No Hit
ATATCAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1010.10649627263045794No Hit
GCTCTTGGGTAAACATTCACCTCATTAACAAATAGATCTTTGAATTTTTATT1000.1054418540895623No Hit
TACGCCGGGGAGAGGATGCCTTTTATCCCCACATTAAAGCACAGATCATTAA1000.1054418540895623No Hit
AGTCAGGGACAGTTAAAACTGTGGATACAGTTAAAACTGATTAGAGGAAGTT1000.1054418540895623No Hit
AAACATGGGATCTTTACAAAAAACAAACAAAAAACCCCCAATTTAATGGGTG990.10438743554866668No Hit
TGACTAGGGCATGTGATTGCTCTCTATTTTACACAGCATGACTTCTTATAAT980.10333301700777106No Hit
CTTAGAGGGGAAGGTATGAAGATAAGACAGGGAAGAGAAGAGATCAGAGGAA980.10333301700777106No Hit
ATCTTTGGGAGTCAGCGAGTGCTTGGGATCCGCATCCAGCCGTGCTGAGCAC970.10227859846687543No Hit
GATATAGGGAATGAAAATGTTCTTGAATCTTCACTTTGACTTTCAGTTTATG970.10227859846687543No Hit
GTGACAGGGTACATAACTAATTTACATTATAAACAACTGTTATTAAACAAAG970.10227859846687543No Hit
GTCTAAGGGATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATAT970.10227859846687543No Hit
GCGGTTGGGTAGAAATTGATTTTCCAGCTCGTGCTATCCTGGAAAAATCTGA970.10227859846687543No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACAACTCGTATGC970.10227859846687543No Hit
TGTAGCGGGAGACTTCAAGGGGTGGGGACACTTAATCCTAATTAACTGGAGG960.10122417992597982No Hit
ACAAAGGGGAGTTCTCAGCTAAAGACAAATTTGTTGCTACAGATCAACATGT960.10122417992597982No Hit
AAAGTAGGGAAGCTTGTGGATGCTCAATAAGGACAGCTGCCGTTTGGACAGA960.10122417992597982No Hit
ATAACAGGGGAATTGTAAATATTTTCTCTCATCCTGTAGGAGTTTTTTCATT960.10122417992597982No Hit
ATTGTGGGGAAGTCATCTTTACCCTGAAGTCATGCCTGAGCTTTTGTAAGGA960.10122417992597982No Hit
CTTAATGGGATATTCACAATTGTGTGTTTTCTCATAAAGCCATTATAATTCA950.10016976138508418No Hit
TTACAAGGGGCACCTGGTGGAGCGAGTGCAGAGGTTTTCACACAACAGAAAC950.10016976138508418No Hit
GCTTCAGGGATTTGGAAGTATAAGTTAAGATGTATGTGTTAAATGTCTTCAG950.10016976138508418No Hit
TTCTGTTGGGGAAGGTATTTTAATTTCCATGTATTTGTATAGTTTCCAAAGT950.10016976138508418No Hit
CTGCAGCTGGGAGAAACCAAAAGATCTAACAATTCTGCTTAGCTTTTTGGTT950.10016976138508418No Hit
TTCTTTGGGAATAAATAACTTTTTTGTTGACTTTCAAGTAAAGTGATTTTTT950.10016976138508418No Hit
TGACCGGGATGGAAACCCAAGGTTATCGAAGATTGGAAGGTTATCATTGTGA950.10016976138508418No Hit
GAGACTGGGAAATGAGTAAATTGGATAAATCTAGATGGGCTTGAGGAAGAAT950.10016976138508418No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)