FastQCFastQC Report
Sat 31 Dec 2022
SRR5848509.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848509.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93985
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC13321.4172474331010267No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT13311.4161834335266266TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC2810.29898388040644786No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA2770.29472788210884715No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2230.23727190509123794No Hit
TAAAGAGGGATTTGTGTCAAATGATTTTTGTATCAAAGCTTTCAGAAAAAGG1450.1542799382880247No Hit
TGAGTAGGGAATTGCCTGATGAATAAATGCATGAGAAATACTAGTTTTAAAG1450.1542799382880247No Hit
AGCAGAAGGATGTTTGTATTAGATGAAACAGACTTCAGAGCAAAGAAAATTA1390.14789594084162366No Hit
GCGCGGGGGAGCTGAACAGAGACTATCATCAGCAAATAGAGCTCAGCTTTGT1360.14470394211842316No Hit
GCTCTTGGGACTGCCATATGGCCTTAGTTTTCTAATTCAAAATTTATTTTCT1350.14363994254402299No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACA1340.1425759429696228No Hit
CAAACAGGGATGAATGAGAAATTAGTTAACATTCTGCTCCACAAGGTAAGAA1320.14044794382082246No Hit
TGGTTGGGAGGAATAAGGAAATTTTAAGTATTGTTTAGGGGCCAAATTTGAA1280.13619194552322178No Hit
AGTCAGGGACAGTTAAAACTGTGGATACAGTTAAAACTGATTAGAGGAAGTT1270.13512794594882163No Hit
TTAAAGGGGGAATCTCACCTTATTGAGTGGGTTCCACTTTATTTTTCATTTG1260.13406394637442146No Hit
GAATTCGGGATGATATTCTTCTTTAGCTTCAATTTATATATCTCATTAAATG1250.13299994680002128No Hit
GAAAATGGGGTGATACACAATAAAACAAGACATGCCTGTAGATTGTTTTTAA1250.13299994680002128No Hit
CTCACTGGGAATATTTTCTCATTCTTTGTGTATAGAAAATTTTAAAATGGTG1240.1319359472256211No Hit
GTCTAAGGGATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATAT1220.12980794807682078No Hit
ATGTTCGGGGAAGAACGTTTGCCAAAAACACTAGTAAAATTCTGACTTGTAA1210.1287439485024206No Hit
CTTTCGGGGGAGATATTAGTATTAAAGTTTTTGGTGGCCTAGAGTGAATTAG1200.12767994892802043No Hit
AGTCAAGGGACATAAGATAATGGTGAAACATTGTTTTTATTTTTATTTTCTT1190.12661594935362025No Hit
ATGCTAGGGAGAATCTACAATGAACTCCAACAAATGTACAAGAAAAAAACAA1180.1255519497792201No Hit
ACTTAAGGGATTTCATACATACCATCAGAAATATGTATGAAAATGATGTGTC1160.12342395063041973No Hit
TGACTAGGGCATGTGATTGCTCTCTATTTTACACAGCATGACTTCTTATAAT1160.12342395063041973No Hit
GTACAAGGGGTATGAGAACTTCTGAAACAGACCTGGTTTTTAGAGATATTTA1160.12342395063041973No Hit
CTACTTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1160.12342395063041973No Hit
TTGATAGGGAGGTTAAATTAATGCCTTCCTTTTTCCTTTATAATCATTTGTT1150.12235995105601959No Hit
GAACCGGGACGGTCAAATCACCTATTGTACCATATTTTGGTGACCATCCCCA1150.12235995105601959No Hit
CAAGAAGGGATAAGAGTTTATATTAAGGACCAAATTCAGTTCCTTGGTCCTT1150.12235995105601959No Hit
ATTAAAGGGCCTTAGCCAGGCAAGCACAGTAATGTGTGTTTTGTTCAGCATT1150.12235995105601959No Hit
AAAACAGGGATCCTGTCTCGCCGTATTGCTGTTGAATATAGTGATTCGGAAG1140.1212959514816194No Hit
AAATGGGTCAATAAGCACATTCAAGAAACTGAATGGCAGAGTCAAGAGGGTA1140.1212959514816194No Hit
CCTTTAGGGCATTTTGAGAATTGCTGTTGATAAGATCCACTGTTTCTTTTCA1130.12023195190721925No Hit
TGGTTTGGGATTTGATCTTGGCTGCCACAAACAGAAAATGTGAGAACATTTT1130.12023195190721925No Hit
TACAAGGGGAGGACGGGAGCTTGGAGAACGTTTTCTAGAAAAGGGTTAGCTT1130.12023195190721925No Hit
GTTCCTGGAGGAGCAGTTTCTCAGTGTTCATCTTCCATGAAGTCGACATTTT1120.11916795233281906No Hit
TACCTGGGTGACAGAGCGAGACTCCATCTCTAAAACAATTAAAAAAGAAAAA1100.11703995318401872No Hit
GAAATTGGGGTTTTATTTAAAACATCTTTCAGTATATAAGCAATTATCCGTG1100.11703995318401872No Hit
TTTACAGGGGGACAGCTTCAACACAGGGTCCACAGCGTGGGGCATTTCCCAG1100.11703995318401872No Hit
GGGCACGGGACTTCACAAGTACACTCGATACCTTGCCTGCTGACAGCTACCC1100.11703995318401872No Hit
GCTAATGGGAAGTGGAAATGAATTTTTAGCTATTATACAACTTTGGAAAATT1090.11597595360961854No Hit
TTTGGCGGGCAATTCTGAATTTGCTTTTCTTGCCTCTCTTTAGTCACCTGTC1090.11597595360961854No Hit
CGTAAAGGGGGGCTGGAGGCGCGGGGCGGGGTTCCGGTGGGCGCGCGTTGAG1080.1149119540352184No Hit
TTGTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1080.1149119540352184No Hit
GTCAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1070.1138479544608182No Hit
ACTGTTGGGACAATAATAGTGGGGGACTTTAGTACTCCACTGACAGCACTAG1060.11278395488641806No Hit
ACTGCTGGGGCACTAGTTTAAATTCCTACCAACAATATGTGAGCATTCCTGT1060.11278395488641806No Hit
GCCTCAGGGGGAAGTTACCCATTTGTAACTTTAAAAACAGGAAAAATATCAG1060.11278395488641806No Hit
AGCCCGGGATGAGCCACCATGCCCAGCCAAAGATGAAAACTTTTAAAAAAGG1050.11171995531201787No Hit
ATAAGCGGGATCTGAACTGGGGATCTATGAGTTTTGAAAAAGTGAATAATCA1050.11171995531201787No Hit
GTAAAGGGGATTAGAATCTATTATGCATAATATATTTTGTGGGAGAAAATCT1050.11171995531201787No Hit
AACACTGGGGGCCAGGGGAGGCGTCTCACCAGACAGAGGTGTAGACGGGCCA1040.11065595573761772No Hit
TTGCACCGGGATAGTTTTAATCTCTTTGTCCCTTTGTGCTGAATTCTGTATG1040.11065595573761772No Hit
GTGACAGGGTACATAACTAATTTACATTATAAACAACTGTTATTAAACAAAG1040.11065595573761772No Hit
CTTAGAGGGGAAGGTATGAAGATAAGACAGGGAAGAGAAGAGATCAGAGGAA1040.11065595573761772No Hit
GAGCCAGGGAAGGCTTTCCTTTGTAACTGCCCTTTCCGTACACAGCAGGCAT1040.11065595573761772No Hit
TATCTAGGGCCGCGCTGGGGCCTGAAACCCCCGGCCTCGGCCCACCAGTTCT1030.10959195616321753No Hit
ATATCAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1030.10959195616321753No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACAACTCGTATGC1030.10959195616321753No Hit
GCCTAAGGGCAGGGTCTCCCTTGGGTATTCATCAGCTTGTTGATCAGTGTAT1020.10852795658881738No Hit
TCGACACGGGATGGCACCTGCATAAAAACCACAGACAGGTAACTTTAGGGAC1020.10852795658881738No Hit
TTCTATGGGCTGAAAGAATTTGTGAAGACTGGTACTATGATTTTTTTCCTTA1020.10852795658881738No Hit
AAATAGGGGAGATTCATATTGTATTCCCTGCAGCAACCACTAAAAAATGCAA1010.10746395701441719No Hit
TACGCCGGGGAGAGGATGCCTTTTATCCCCACATTAAAGCACAGATCATTAA1010.10746395701441719No Hit
TATATGGGGCTTAGATCTCTGAATGTGGAGAATTTAGTTAACATTTTTTGTT1010.10746395701441719No Hit
CGTAGCGGGAGGAGAAAAAGGATGTGCCACACCAACTATATTGATGAAGTCA1000.10639995744001701No Hit
TACGGTGGGGACCACAAGTGTAAGACTGAATGAAAGAATAAATGCGTGCATT1000.10639995744001701No Hit
ATTGCTGGGCATACTCCCATAAGAATTTTGACGTATTTATCTGCTCTCAAGT1000.10639995744001701No Hit
TATAAAGGGACTGGTGGTGATAAGAGGAGCCGTCTGGTGTGTCAGGGTCACG1000.10639995744001701No Hit
TCACTTGGGAACTTTCAGGCTGACAACTCTGCTGATGTCTGGCTGTTCATCA1000.10639995744001701No Hit
TCCGCAGGGAAGAGATTAAAAATTAAAGAAGCTATTTCATTGAATATTATAC1000.10639995744001701No Hit
AACCTTGGGTGGAGCTGGAAAGAAAAATTCTTTTTCTGTACCTCATGCCTAT1000.10639995744001701No Hit
TTGTGGGGGCAAGCAGGGCTATATTTCAGTGACAGCTGATATTTGTTTTGAA990.10533595786561686No Hit
ACCTGTGGGAAGCACTGAAACTACTGCCACCTGCTCACTGCGAAACCCTCCG990.10533595786561686No Hit
TATTGAGGGGTCTACTAGACTTTCTTACAGATTTAATTTCTTTTGTATTTTA990.10533595786561686No Hit
TTCGCAGGGAATTTCGTGTATGCTCATTTTTAATTTAAATATGGTTAACATC990.10533595786561686No Hit
TCCTAGGGGTGTGTCCTGCAAGCTCTATGACGTTATGCACAGCGTTTACTCT990.10533595786561686No Hit
TCCGAACGGGGAGCCACCGCGCCTGGCCAAAATAAAATGTTTTTATCTTTCT980.10427195829121667No Hit
AATACAGGGATTTCTTCAAGGAGAAGAGAAGATCAGTCTTTAAGGCTTAATG980.10427195829121667No Hit
ACTTGTGGGCGATCTCAGGCCGGGAGGCGCCCACCACCACGCCTCGCTAATT980.10427195829121667No Hit
AGTCAAGGGCTAAAACATTATGTAAAATATGTAAAATTCTGCATTATTAAAT980.10427195829121667No Hit
TTCTGTGGGACTTACAGTCGGTAACCTCTATTGAAGAACGAACAAGGCCAAA980.10427195829121667No Hit
AGTTGCGGGGCCATAACACATGATGTCATCAATAACCTACTGAAATTTATTC970.10320795871681653No Hit
TACGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC970.10320795871681653No Hit
ACTATTGGGAAGGGGACAAAAGATGGGATGTGAAAAGAAGAATTCTAGTTTG960.10214395914241634No Hit
ACGCGGGGAAGTTTCGGTTGAACACCTTTGGAAGCCAAGTCCTCATATTTAA960.10214395914241634No Hit
TATAGGGGGCTGGCTGAAGCATCCCCTTGGAGTGCCATGTATAAGTTGGGCT960.10214395914241634No Hit
TCACGAGGGTCTGCAGGCGGGACCGCTTGATTCACCTGACTCCTGCGGACTA960.10214395914241634No Hit
GCCTTTGGGATCGACTGGGTTTTAATGGCCTAGTTATTTGAGGATTTTGCTG960.10214395914241634No Hit
AAATACGGGAGTGTGTCATCTTAGTTTATCACTGCCATGGCTACACCCAGAA960.10214395914241634No Hit
ATGTATGGGGCAAAATGGCGGCGGCGATGACCTTCTGCCGGCTGCTGAACCG960.10214395914241634No Hit
ACTCAAGGGAATGTGTGTGTAACACTGAAGCTATGCATTTGAAGAGCTCTGA950.10107995956801619No Hit
ACTTATGGGAAATGTAATAATGTATTACAATTAAATGTCTGAATAAAAAAGT950.10107995956801619No Hit
CAAGGAGGGATGAATAATGTCATGAATGCTCAGGCTAAAAAGAGTCTTGATC950.10107995956801619No Hit
TGTAGCGGGAGACTTCAAGGGGTGGGGACACTTAATCCTAATTAACTGGAGG950.10107995956801619No Hit
GATATAGGGAATGAAAATGTTCTTGAATCTTCACTTTGACTTTCAGTTTATG950.10107995956801619No Hit
GTTTACGGGCAATATTTCATTTAAAGAACTGTATTAGTCCATTCTCACACTG950.10107995956801619No Hit
AATGCAGGGTGGCTTGCTTTTGGAAAAAAAATTTTAAGATTTATTCTGCTAG950.10107995956801619No Hit
ACGTCTGGGAAATGAAAATGATTACTTGGGGAATGGGGAGAAAGAATAGGTA950.10107995956801619No Hit
GTGAAGGGAGAGACAGATCACAGAAAAGAGACAGTGGATCTTTCTACCTTGT950.10107995956801619No Hit
TTAACTGGGTGGAAAGAAACCCCGAGCTAGAATTTCAAGACAAAGTAGATCT950.10107995956801619No Hit
ACAAAGGGGAGTTCTCAGCTAAAGACAAATTTGTTGCTACAGATCAACATGT940.100015959993616No Hit
ATATTCGGGACTGTCAAGATTAAGTTATACCAGCAAAAGTGCAGTAGTGTCA940.100015959993616No Hit
ATCCTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA940.100015959993616No Hit
AACGCGGGGGAAATAAATATTTAACTGGTTATCTTTCCAGTAAGTACAAGGT940.100015959993616No Hit
ATAACAGGGGAATTGTAAATATTTTCTCTCATCCTGTAGGAGTTTTTTCATT940.100015959993616No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)