FastQCFastQC Report
Sat 31 Dec 2022
SRR5848508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94233
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC12701.3477231967569747No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT11971.2702556429276368TruSeq Adapter, Index 11 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA3100.3289718039328049No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC3030.3215434083601286No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2430.25787144630861797No Hit
AGCAGAAGGATGTTTGTATTAGATGAAACAGACTTCAGAGCAAAGAAAATTA1570.1666083007014528No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACA1430.1517515095561003No Hit
TTGATAGGGAGGTTAAATTAATGCCTTCCTTTTTCCTTTATAATCATTTGTT1420.15069031018857512No Hit
GCTCTTGGGACTGCCATATGGCCTTAGTTTTCTAATTCAAAATTTATTTTCT1340.14220071524837372No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACAACTCGTATGC1310.13901711714579817No Hit
TGAGTAGGGAATTGCCTGATGAATAAATGCATGAGAAATACTAGTTTTAAAG1300.13795591777827299No Hit
GCGCGGGGGAGCTGAACAGAGACTATCATCAGCAAATAGAGCTCAGCTTTGT1290.13689471841074782No Hit
GAAAATGGGGTGATACACAATAAAACAAGACATGCCTGTAGATTGTTTTTAA1280.13583351904322263No Hit
GTCAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1260.13371112030817228No Hit
CTTTCGGGGGAGATATTAGTATTAAAGTTTTTGGTGGCCTAGAGTGAATTAG1230.13052752220559677No Hit
TAAAGAGGGATTTGTGTCAAATGATTTTTGTATCAAAGCTTTCAGAAAAAGG1210.12840512347054642No Hit
TTAAAGGGGGAATCTCACCTTATTGAGTGGGTTCCACTTTATTTTTCATTTG1210.12840512347054642No Hit
TTCTGTGGGACTTACAGTCGGTAACCTCTATTGAAGAACGAACAAGGCCAAA1200.12734392410302123No Hit
TTTGGCGGGCAATTCTGAATTTGCTTTTCTTGCCTCTCTTTAGTCACCTGTC1190.12628272473549607No Hit
TTCGCAGGGAATTTCGTGTATGCTCATTTTTAATTTAAATATGGTTAACATC1180.12522152536797088No Hit
ATGCTAGGGAGAATCTACAATGAACTCCAACAAATGTACAAGAAAAAAACAA1150.12203792726539534No Hit
CTCACTGGGAATATTTTCTCATTCTTTGTGTATAGAAAATTTTAAAATGGTG1150.12203792726539534No Hit
ACTGTTGGGACAATAATAGTGGGGGACTTTAGTACTCCACTGACAGCACTAG1140.12097672789787017No Hit
ATCCTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1140.12097672789787017No Hit
GAATTCGGGATGATATTCTTCTTTAGCTTCAATTTATATATCTCATTAAATG1130.11991552853034501No Hit
TCGACACGGGATGGCACCTGCATAAAAACCACAGACAGGTAACTTTAGGGAC1130.11991552853034501No Hit
AAATAGGGGAGATTCATATTGTATTCCCTGCAGCAACCACTAAAAAATGCAA1120.11885432916281982No Hit
AAACATGGGATCTTTACAAAAAACAAACAAAAAACCCCCAATTTAATGGGTG1120.11885432916281982No Hit
CAAACAGGGATGAATGAGAAATTAGTTAACATTCTGCTCCACAAGGTAAGAA1120.11885432916281982No Hit
AAAACAGGGATCCTGTCTCGCCGTATTGCTGTTGAATATAGTGATTCGGAAG1110.11779312979529465No Hit
CAAGTTCTGCTTCCCCTAAGTGAGTTCTTGAAGATAAATTTAACAGTTGGCA1110.11779312979529465No Hit
CCTTTAGGGCATTTTGAGAATTGCTGTTGATAAGATCCACTGTTTCTTTTCA1110.11779312979529465No Hit
TGACTAGGGCATGTGATTGCTCTCTATTTTACACAGCATGACTTCTTATAAT1110.11779312979529465No Hit
CTACTTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1110.11779312979529465No Hit
AAATGGGTCAATAAGCACATTCAAGAAACTGAATGGCAGAGTCAAGAGGGTA1110.11779312979529465No Hit
TCCTAGGGGTGTGTCCTGCAAGCTCTATGACGTTATGCACAGCGTTTACTCT1110.11779312979529465No Hit
GCTCTTGGGTAAACATTCACCTCATTAACAAATAGATCTTTGAATTTTTATT1100.11673193042776946No Hit
AGTCAAGGGACATAAGATAATGGTGAAACATTGTTTTTATTTTTATTTTCTT1100.11673193042776946No Hit
TACGGTGGGGACCACAAGTGTAAGACTGAATGAAAGAATAAATGCGTGCATT1080.1146095316927191No Hit
TATAAAGGGACTGGTGGTGATAAGAGGAGCCGTCTGGTGTGTCAGGGTCACG1080.1146095316927191No Hit
TTCTATGGGCTGAAAGAATTTGTGAAGACTGGTACTATGATTTTTTTCCTTA1070.11354833232519394No Hit
GCCATTGGGGTTAGATTTTGCATGAAATGGTTCTGAAAGGTAAGAGGAAAAC1070.11354833232519394No Hit
AGTCAGGGACAGTTAAAACTGTGGATACAGTTAAAACTGATTAGAGGAAGTT1070.11354833232519394No Hit
ATATCAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1050.11142593359014358No Hit
ACGCGGGGAAGTTTCGGTTGAACACCTTTGGAAGCCAAGTCCTCATATTTAA1040.11036473422261839No Hit
CAGATGGGGACACCTGCCTGCATTTAACATAACTCGATTTTTTTCCCTCCCT1040.11036473422261839No Hit
ACATCCGGGAATTATAATGGATGTGGTAAGGCTTTTTAAAAAATCAATGTTC1040.11036473422261839No Hit
TTGTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1040.11036473422261839No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1040.11036473422261839No Hit
ACTAAAGGGCACTCACTCACTTCCAATCCCTCTCAGATGAGCTTCTTTTAAT1040.11036473422261839No Hit
ATAACAGGGGAATTGTAAATATTTTCTCTCATCCTGTAGGAGTTTTTTCATT1040.11036473422261839No Hit
TGGTTGGGAGGAATAAGGAAATTTTAAGTATTGTTTAGGGGCCAAATTTGAA1040.11036473422261839No Hit
GTGAAGGGAGAGACAGATCACAGAAAAGAGACAGTGGATCTTTCTACCTTGT1040.11036473422261839No Hit
TTAACTGGGTGGAAAGAAACCCCGAGCTAGAATTTCAAGACAAAGTAGATCT1040.11036473422261839No Hit
ACTGCTGGGGCACTAGTTTAAATTCCTACCAACAATATGTGAGCATTCCTGT1030.10930353485509323No Hit
CATGCTGGGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC1030.10930353485509323No Hit
GTTTACGGGCAATATTTCATTTAAAGAACTGTATTAGTCCATTCTCACACTG1030.10930353485509323No Hit
GCCTTTGGGATCGACTGGGTTTTAATGGCCTAGTTATTTGAGGATTTTGCTG1030.10930353485509323No Hit
GATATAGGGAATGAAAATGTTCTTGAATCTTCACTTTGACTTTCAGTTTATG1020.10824233548756806No Hit
TATAGGGGGCTGGCTGAAGCATCCCCTTGGAGTGCCATGTATAAGTTGGGCT1020.10824233548756806No Hit
ATAGCTGGGTTTCAAATCTTATTGTTATCATGTTATAAAATTTTAGCCCACA1010.10718113612004287No Hit
GTGACAGGGTACATAACTAATTTACATTATAAACAACTGTTATTAAACAAAG1010.10718113612004287No Hit
ACTTAAGGGATTTCATACATACCATCAGAAATATGTATGAAAATGATGTGTC1010.10718113612004287No Hit
AATACTGGGAGGAAGACTGAAAGGTTGACCCTGATAGTCCTTTGCACAGTGA1010.10718113612004287No Hit
ATATATGGGCCGATCTGACCGAGGGAGTGATTAGGATGGATTGAAGCATGTA1010.10718113612004287No Hit
TCGCTAGGGGAACACAACACAATAACAAAACTGGTAACCATCGAGCAAAAGT1000.1061199367525177No Hit
ATGTTCGGGGAAGAACGTTTGCCAAAAACACTAGTAAAATTCTGACTTGTAA1000.1061199367525177No Hit
TCCTTGGGGTAACAGATTTTTCTGATGAGCATGTTTTATGAATCCTCCATTG990.10505873738499251No Hit
AAAGTAGGGAAGCTTGTGGATGCTCAATAAGGACAGCTGCCGTTTGGACAGA990.10505873738499251No Hit
GTCTGGGGGAAACATTGTTTTCTGTTAAGAAGGGAGGAATACCATATATAAT990.10505873738499251No Hit
TGGTTTGGGATTTGATCTTGGCTGCCACAAACAGAAAATGTGAGAACATTTT990.10505873738499251No Hit
ACTCATGGGGGCACATATTAATACAGTTTTTATATGGTATCTATGAAAGAGT990.10505873738499251No Hit
GGAACTGGGAGTCACTAACAGCCATAAATGATAACTAAGAAATATGTGACCA990.10505873738499251No Hit
GCCTAAGGGCAGGGTCTCCCTTGGGTATTCATCAGCTTGTTGATCAGTGTAT980.10399753801746735No Hit
AGTTGCGGGGCCATAACACATGATGTCATCAATAACCTACTGAAATTTATTC980.10399753801746735No Hit
TCAGTTGGGGATTTTCACACTGGCTGCTCTCCCTCAGTATGAAGCCTTTAAG980.10399753801746735No Hit
TCGATTGGGAGTTCTGAATATTAGTTGTTTGTTTATAGTTGGTAGGTGCTTT970.10293633864994216No Hit
CAAGAAGGGATAAGAGTTTATATTAAGGACCAAATTCAGTTCCTTGGTCCTT970.10293633864994216No Hit
GTACAAGGGGTATGAGAACTTCTGAAACAGACCTGGTTTTTAGAGATATTTA970.10293633864994216No Hit
TATTGGGGGATATCATATAGAACTTTTAGCATCCTGGGAAGAGCTGCCCCCA970.10293633864994216No Hit
TCACTTGGGAACTTTCAGGCTGACAACTCTGCTGATGTCTGGCTGTTCATCA970.10293633864994216No Hit
GGCTTCGGGATGTCTTATGAAGGAAGAGACAATAATGAGATATTTCATTAAT970.10293633864994216No Hit
CAAGGAGGGATGAATAATGTCATGAATGCTCAGGCTAAAAAGAGTCTTGATC960.101875139282417No Hit
GAACCGGGACGGTCAAATCACCTATTGTACCATATTTTGGTGACCATCCCCA960.101875139282417No Hit
CTCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG960.101875139282417No Hit
ACTATAGGGATCAGCTGTAAAGAATATGAATCTTCCTGAGATCCCAAGAAAT960.101875139282417No Hit
TACAAGGGGAGGACGGGAGCTTGGAGAACGTTTTCTAGAAAAGGGTTAGCTT960.101875139282417No Hit
GTAAAGGGGATTAGAATCTATTATGCATAATATATTTTGTGGGAGAAAATCT950.1008139399148918No Hit
TACGCCGGGGAGAGGATGCCTTTTATCCCCACATTAAAGCACAGATCATTAA950.1008139399148918No Hit
ACTTGTGGGCGATCTCAGGCCGGGAGGCGCCCACCACCACGCCTCGCTAATT950.1008139399148918No Hit
CGATTTGGGCTTGGAAAGACGAAAAGCATCCTGGGTTCAGGCTGACATCTGC950.1008139399148918No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)