FastQCFastQC Report
Sat 31 Dec 2022
SRR5848501.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848501.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171234
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC15840.9250499316724482No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT14210.8298585561278718TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC4270.24936636415665114No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA3910.2283425020731864No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2380.1389910882184613No Hit
TTGACCGGCTACGTGCGGGGGGAGAGAAGGGCACAGACTGGCTCCCCTCGCC2030.11855122230398167No Hit
CCATCCGGGGCACTTTCTCATGAGTGTTTAGAAGGAGAGAATCTGTCGTGGC2020.11796722613499656No Hit
AACTGCGGGGTAAGAAGGCCTTCCGTTGGGGCAATCAGCTTACTCAACATCA1980.11563124145905603No Hit
GCTATAGGGACAATTGGTGTGGATTTCAAAATAAGAACTATAGAGTTAGACG1920.11212726444514524No Hit
GACCAAGGGAACTGCCAGATGGCCAGAAAGAAGGCAACAGTGGACTCAGACT1900.11095927210717497No Hit
TTTTTAGGGAAGGATCCAAGATATGCCAGATATCTCAAAATGGTTCAAGTGG1890.11037527593818984No Hit
AAGTTTGGGCTTACTTTAGAGGAAAAGAAACTGAGGCAAAGAACATTTAAAT1860.10862328743123445No Hit
TGAGTTGGGGATTCAAAGGTCATCTAAGTCAGTTCAGTCTTTGCAAAAAGAA1860.10862328743123445No Hit
CGTGAGGGGAGACTGAAATTATGGCCCATGATGCATTCCATTTTAACTCTTT1860.10862328743123445No Hit
CCTAGGGGATGAGGAAAACTGTAAAATTCTAATGAAAGAAATTCAAGAACAC1850.10803929126224932No Hit
TATGCAGGGAGAGGAGGATGCAAACAAAGTTAGCAGTTAGTTTTATCATTTG1840.1074552950932642No Hit
TTAGGAGGGTGCTTAGAAATGACTTCTGCCGGCTGAGTGTGGTGGCTCACGC1830.10687129892427906No Hit
TAACAGGGAGAGTTTGTGGTTGCTACCATCTTGTGTCCCCTTCTCCCATTTA1820.10628730275529392No Hit
TGCTTGGGGGGCCGTCGCTCGCTCCCTTCTCGGCAGTGGTACCTGTTCCCGG1810.10570330658630879No Hit
GTTGCCGGGAGTGCGAATCATGGGAGGCGGCTGGTGGTGGGCTCGGGCCGCT1800.10511931041732367No Hit
TTTTTTGGGCTCTTCCGATCTTAGACTGGGATGACCCCAATACGCAAAACTA1790.10453531424833853No Hit
TAGCCTGGGGGGGTTATTAGGTTGGGAGAAATGTTGATACTTTTGTTTTGTG1780.1039513180793534No Hit
TGTTAGGGGACTTGTAGCTTGTTCATTCCCTCATGTGACCAACCACCTTGGT1780.1039513180793534No Hit
TTATCTGGGCAAAAGGTAACATTCTATATGCTAGTCTCTTGTCTTTAGTACT1770.10336732191036827No Hit
GTCAATGGGACTCTTTGAGCCCAGCAATGGCACCCTGCTGGCGGTCATGGAT1770.10336732191036827No Hit
TTCGATGGGTCTTGGACTGGGGCTGATACTTGAGGTTATGCTTATTAAAAAC1770.10336732191036827No Hit
TCATGCAGGGATCTCACAGATGAGGAGAGAGAAGAAACGGAAGAGGAAGTTT1760.10278332574138313No Hit
CAACAAGGGGGAATGTTTTCATTTTTTTCTGCCTGGTCCTAAAGTATTAGCA1760.10278332574138313No Hit
TCTATAGGGCTAACACCCATTCCTGGTTTTTTAAAAAAACTCTCAGTGAACT1760.10278332574138313No Hit
TCGCAGAGGACCCCTGGTCAATCAATGGCCCTCACTGGGAACGCTTAATGGG1760.10278332574138313No Hit
CCCAAGGGGAATGATCTTACTCTTTCAAGGAATGACTTCATCTTGTCCGTTT1750.10219932957239801No Hit
AAAACAGGGGAACAATGTGGAAAGCAAACTTATTCCAAGTTTCCTAAAATGA1750.10219932957239801No Hit
ATCGCAGGGGAATTTTTGAAATCCAAAATAAGTATAAAATCATAGAGAATAT1740.10161533340341287No Hit
ATTCTTGGGGATTGATCAGAAGAAACACTCCAAAAATTGAGATGAAATGTTG1740.10161533340341287No Hit
AACTCAGGGCCATTTTTAAGTTATATTTCTTTGATTTTTGTTAATTTAGAGG1730.10103133723442775No Hit
ATGCACGGGGCTGGTTTTGATTTTCATGGAAACTTTTTCTTCTTTTCAAGAC1730.10103133723442775No Hit
CGTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1720.10044734106544262No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)