FastQCFastQC Report
Sat 31 Dec 2022
SRR5848500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170578
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC15420.9039852735991746No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT12990.7615284503277093TruSeq Adapter, Index 11 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA4020.23566931257254747No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC3760.22042701872457177No Hit
TTGACCGGCTACGTGCGGGGGGAGAGAAGGGCACAGACTGGCTCCCCTCGCC2390.14011185498716128No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2260.13249070806317345No Hit
TTAGGAGGGTGCTTAGAAATGACTTCTGCCGGCTGAGTGTGGTGGCTCACGC2150.12604204528133758No Hit
TGAGTTGGGGATTCAAAGGTCATCTAAGTCAGTTCAGTCTTTGCAAAAAGAA2100.12311083492595762No Hit
GACCAAGGGAACTGCCAGATGGCCAGAAAGAAGGCAACAGTGGACTCAGACT2040.11959338249950169No Hit
TCATGCAGGGATCTCACAGATGAGGAGAGAGAAGAAACGGAAGAGGAAGTTT1930.1131447197176658No Hit
TGCTTGGGGGGCCGTCGCTCGCTCCCTTCTCGGCAGTGGTACCTGTTCCCGG1930.1131447197176658No Hit
GTAGCTGGGCAAACTTCCAAAGGTCAAGGATAATGAAAGGATCCTAAAAGCC1900.11138599350443786No Hit
GGTAGAGGGACCAGTTGGCAGACAGCCGTTTGTTCTACATGGCTTTGATAAT1890.11079975143336185No Hit
GTAGGAGGGGAATTTAACAGGAAGCCAGCTGGCAAAGGGGTCTCAGAAATGT1880.11021350936228588No Hit
ACTCTAGGGATTATAACTTCAGAAAAACATAAAAGTCCTTTTAAGAAGAAGT1870.10962726729120988No Hit
GCTTAGGGGACAGTGAAGAAGAAATTAGAGAAGCATTCCGTGTGTTTGATAA1850.1084547831490579No Hit
TTTTCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1850.1084547831490579No Hit
TGCATAGGGAGAAAGATAATGGTTTCCTAGGAGTTTGCATTCTTAGACCACA1850.1084547831490579No Hit
AAGTTTGGGCTTACTTTAGAGGAAAAGAAACTGAGGCAAAGAACATTTAAAT1840.10786854107798192No Hit
TTATCTGGGCAAAAGGTAACATTCTATATGCTAGTCTCTTGTCTTTAGTACT1840.10786854107798192No Hit
TACTCTGGGGAGTAGGAAGACACTAGGTAAATATGTACGTGAAGTTAGTTTT1830.10728229900690593No Hit
TTCGATGGGTCTTGGACTGGGGCTGATACTTGAGGTTATGCTTATTAAAAAC1820.10669605693582995No Hit
AGCTAAGGGAAAAGCCTAATATTTATTTGTATGTCAATATTTTTCATTTGAT1800.10552357279367797No Hit
GACACAGGGTGTGAATTTAAGTGAACATTTGCCTCAAGATGTTAACTATAAA1800.10552357279367797No Hit
AGAACTGGGTCTAAACATTTGAAATTGTCTTTCAAAGATGTTTAATATTTGA1800.10552357279367797No Hit
AGCACAGGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGG1790.10493733072260197No Hit
TAACGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1780.104351088651526No Hit
GTAACCGGGTGACCAAGAAAAAGTAGTTCTTTTGTGCACGCGTGAATACATC1780.104351088651526No Hit
AGGCAAGGGTATTGCTTTGCCATTTAAAAATCCAGCCATGAAAGGAATTTTG1770.10376484658045No Hit
ACGTGCGGGCTTGAAGGGGGAAAGCTGGTCCTAGAACCTGGGTCTTCAGAGC1770.10376484658045No Hit
CTTCAAGGGATTCCAGAAGTACAACATAATCTTATTAATAGCATCTCCCTTT1770.10376484658045No Hit
TATTTTGGGTAAGTGAAAAACCTTTGTGTAAAACAGGTGAGTGGAGAAGACG1760.10317860450937402No Hit
ATTCTTGGGGATTGATCAGAAGAAACACTCCAAAAATTGAGATGAAATGTTG1760.10317860450937402No Hit
GTTGCCGGGAGTGCGAATCATGGGAGGCGGCTGGTGGTGGGCTCGGGCCGCT1750.10259236243829802No Hit
CCTAGGGGATGAGGAAAACTGTAAAATTCTAATGAAAGAAATTCAAGAACAC1750.10259236243829802No Hit
TTTTTTGGGCTCTTCCGATCTTAGACTGGGATGACCCCAATACGCAAAACTA1740.10200612036722204No Hit
CGTAACGGGTTCAGCAGAGGAGTATTCTCTCAAAAATGCTCAGTCTCTCCCA1740.10200612036722204No Hit
GTAGGGGGGCAGAATTTAGTGTTATCAGTGATCTGGATTTTGGTATATAGTA1740.10200612036722204No Hit
GAACTAGGGATACACACAAAACAGCAAACTTCAGGTAACTATTTTGGATTGC1730.10141987829614604No Hit
TAGCCTGGGGGGGTTATTAGGTTGGGAGAAATGTTGATACTTTTGTTTTGTG1720.10083363622507006No Hit
GATATTGGGACTTGAACAAAGAAAAATGTTACATATATCTAAAGGAATTAGT1720.10083363622507006No Hit
CGTCGCGGGAGAATGCAAGCTACAAGAAACAGTTAAGGGTACCTGTTACGAA1710.10024739415399407No Hit
GATTTTGGGAAGGAGGATAAGAGAGTTATTATTATCCCCATTTTCCAAATGA1710.10024739415399407No Hit
GCTATAGGGACAATTGGTGTGGATTTCAAAATAAGAACTATAGAGTTAGACG1710.10024739415399407No Hit
TTGGTTGGGATCTGGACAGGGGGGATGTGCTGGATTTGGTACCCGTAGGCCT1710.10024739415399407No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)