FastQCFastQC Report
Sat 31 Dec 2022
SRR5848499.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848499.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170529
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC15010.8802021943481755No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT13880.8139378052999783TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC4190.24570600894862457No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA3420.2005523987122425No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2250.13194236757384376No Hit
CCTAGGGGATGAGGAAAACTGTAAAATTCTAATGAAAGAAATTCAAGAACAC2060.12080056764538583No Hit
ACTCTAGGGATTATAACTTCAGAAAAACATAAAAGTCCTTTTAAGAAGAAGT2040.119627746600285No Hit
TCTATAGGGCTAACACCCATTCCTGGTTTTTTAAAAAAACTCTCAGTGAACT1990.1166956939875329No Hit
TGAGTTGGGGATTCAAAGGTCATCTAAGTCAGTTCAGTCTTTGCAAAAAGAA1960.11493646241988167No Hit
AATACTGGGAAGTGTTTTGTTGTAGCTTAGTATCCATAAGGGAAACTTAGAC1950.11435005189733125No Hit
TATGCAGGGAGAGGAGGATGCAAACAAAGTTAGCAGTTAGTTTTATCATTTG1930.11317723085223041No Hit
GCTATAGGGACAATTGGTGTGGATTTCAAAATAAGAACTATAGAGTTAGACG1900.11141799928457916No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACA1890.11083158876202875No Hit
AAGTTTGGGCTTACTTTAGAGGAAAAGAAACTGAGGCAAAGAACATTTAAAT1850.1084859466718271No Hit
TTATCTGGGCAAAAGGTAACATTCTATATGCTAGTCTCTTGTCTTTAGTACT1830.10731312562672624No Hit
TTGGTTGGGATCTGGACAGGGGGGATGTGCTGGATTTGGTACCCGTAGGCCT1830.10731312562672624No Hit
GTAAGTGGGGTTTTCCTTTTTTAAGATGAAAGTAAACATAAATAGAGATGGT1830.10731312562672624No Hit
TTGACCGGCTACGTGCGGGGGGAGAGAAGGGCACAGACTGGCTCCCCTCGCC1830.10731312562672624No Hit
GTCTGGGGGCACTAGATTGACGAGGGCTTTTAGAGACTGCTCCTATAATTGA1830.10731312562672624No Hit
GACCAAGGGAACTGCCAGATGGCCAGAAAGAAGGCAACAGTGGACTCAGACT1800.105553894059075No Hit
AACTGCGGGGTAAGAAGGCCTTCCGTTGGGGCAATCAGCTTACTCAACATCA1790.10496748353652458No Hit
TGAAAGGGTAGTGTTGGTGGTATGTAACAAGAGTTACGTATCCAGGGGCTTG1780.10438107301397417No Hit
TATTTTGGGTAAGTGAAAAACCTTTGTGTAAAACAGGTGAGTGGAGAAGACG1770.10379466249142374No Hit
CCATCCGGGGCACTTTCTCATGAGTGTTTAGAAGGAGAGAATCTGTCGTGGC1770.10379466249142374No Hit
CGTGATGGGCATCTCCACTTACCACAACTTGTGCCTCCTGGGTTCAAGCGAT1750.10262184144632291No Hit
TTAGGAGGGTGCTTAGAAATGACTTCTGCCGGCTGAGTGTGGTGGCTCACGC1750.10262184144632291No Hit
TGTTAGGGGACTTGTAGCTTGTTCATTCCCTCATGTGACCAACCACCTTGGT1730.10144902040122208No Hit
TATATGGGGTCATTTTTTCCTCTAATTATTCAAGACTGGCACAAAAGTATAA1730.10144902040122208No Hit
TTCGATGGGTCTTGGACTGGGGCTGATACTTGAGGTTATGCTTATTAAAAAC1710.10027619935612125No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)