FastQCFastQC Report
Sat 31 Dec 2022
SRR5848495.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848495.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84174
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC11131.3222610307220757No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT10301.2236557606861975TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC2980.3540285598878513No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA2720.32314016204528717No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2220.2637393969634329No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACA1430.16988618813410317No Hit
GGTACCGGGAATCAGTTAGTGGAGTGAAAATGCCAAATCCTATGGATTTCTC1150.1366217596882648No Hit
GGCTACGGGAGGTTTTTCTATGAAAGTAAAAGTGGGGGAGTATATTTAAAGA1120.13305771378335352No Hit
GCTGTCTCTTTCCTTTCTTAAAACCTTAAGGTCTTTCTAGAGACCTATTCAA1100.13068168318007936No Hit
TGTGTTGGGGAAGCAGGATAAATAAAATCTCTAATCTAGATACATCAAAGAC1090.12949366787844227No Hit
GGTAGCTTGGAGTCGAAGGGTGGTAGTACCCGAGCTATCAGCTGGTGTAGCC1050.12474160667189393No Hit
GTCACTGGGGCTCAATAAAATGCTGGCAAACTGAATTCAGCGGTATATCAAA1030.12236557606861975No Hit
ATGGCAGGGTGGACCGGGTTCCAGACCAAGGCTGAAGTGGAGCAGGTGGAGC1000.11880153016370851No Hit
TTTAATGGGAGGAGATCAGAAATGGTTCTATTTCAAGGTCTCTCACAGGGCT990.11761351486207143No Hit
GATATAGGCAGAAGAATTTTAAGGTAATGACAGTTTTACACCATCTGTCTTA990.11761351486207143No Hit
AGGTTAGGGGAAAAAATGAATGGAATGCTCCTAAATGATCGCAAAGTATTTG960.11404946895716017No Hit
AAACTCGGGCTGCTTGAGGGAACTGCTAGCCATGACTCAAGACTCAGCTCAA960.11404946895716017No Hit
TCTAATGGGATCAATGGAAAGAGGAAGACCGCCAAAGAACATCTGAGGAAAC950.11286145365552308No Hit
CTTTGTGGGCAAAACAAAAATGAATTCAGTTGTGTAATTCTTGTTAGCAAAT940.111673438353886No Hit
ACACAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA940.111673438353886No Hit
TATCTTGGGATGCAGAAAATAGTGAGATTAAGATCGTCCTTATTGTTTCTAA940.111673438353886No Hit
GCTATAGGGGGAGTTTACAATCCAGTGTCAAGAAATGAGAAGCAAAGAAGTA940.111673438353886No Hit
CGCTTTGGGCAACTTAGTACATTAAAGCTACGAAAACTCATCCTGGCTGTAG940.111673438353886No Hit
TACAGGGAGTTACATGTAATTTTTTCGTTATACTTCAGTAAATTTCTGCTTT930.11048542305224891No Hit
GTTTTAGGGTCTGTCGGTGGGTATACATGTAATTAATCTAAAATTAAGTGTT920.10929740775061184No Hit
CACTCAGGGTGATTTCTACCAAATATTTAAAGAAAAATTCATGCAAACAAAC920.10929740775061184No Hit
TTTCAGGGGAATGATGAATGTTGAATGATGCACTATGTTTTTGTTTAAATGA920.10929740775061184No Hit
GCTTGGGGGAGTATTTTGTGGACCAAATTACAATAGGTGGTATTGTACATAA910.10810939244897473No Hit
AGCCGAGGGAAGCAGCAAAATGCCTTTACGTTGTTCTAAACCCCCGACGCAT910.10810939244897473No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGT900.10692137714733765No Hit
CAATTTGGGGTAAAGTTCAAAGCAATCTTTAAAAGAATGAAGACTTTCAGCC900.10692137714733765No Hit
AGCTACGGGTCTGAACTTAATTCAATGATTTTAATTTAGGTACTCAGAAACA890.10573336184570058No Hit
TGCTAAGGGTGAAATGACACAGGAGCCAACTTGAAGGGACTCCCATTAACCA890.10573336184570058No Hit
CGTTGCGGGCTGAAGACAAAGTTATAGAGTTTTTCTTCTTGCTGTAATTGAT880.10454534654406349No Hit
TTGTATGGGTGACAAAGCAGTTGGATTTGGCATGTGTGATGAAAACAATTAG880.10454534654406349No Hit
ATAGGCGGGGCCTCCTGACTTTTAAGGAAAACGTGTGCAGCCATTACACACA880.10454534654406349No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACAACTCGTATGC880.10454534654406349No Hit
GGCACTGGGGGTACGTACAGCTTATTCCTCCTTTAATTTGCTTAGGGGAGGG870.10335733124242641No Hit
ACATTGGGGTACAAGAAAACAAAAATCAGTCAAAAGATTTGGCTGTTCATCT870.10335733124242641No Hit
AAGACGGGGCTTGATTTCAGATGCTATATTAGTTTTCTTTGGTTAAGTAACA870.10335733124242641No Hit
ACCATGAGGAACCTTGAAACTCCATGTTTTGTGTCTAATGACTTCTAGGACA870.10335733124242641No Hit
ACGTTGGGGGAGCATTTGAAGGTCTTTGTCAAGGTTGTTTCTGTTTCTGATC860.1021693159407893No Hit
TGTGCTGGGTAGTTCCTTGTTCCTTGACTGGCCTCTGGCTCTCACCACGTAG860.1021693159407893No Hit
TTGCTGGGGGCTTAACTGTTGTGTATCTTTTTTGTTCTTTACAAGAAGTGCA850.10098130063915223No Hit
AAAGCTGGGCAAGGGACTAATATCCAAATATACAAGGAACTCAAACATCTCA850.10098130063915223No Hit
GTCTATGGGCAATCTTTGTATCTATGCATGCATGTGATAGTGTACATGTGTA850.10098130063915223No Hit
CATAAAGGGAAATAATAAAAGACAAATTATCTTGTCAATTCTCTATTTCTAG850.10098130063915223No Hit
ATGCATGGGAGAACTGTAGACACCAAAATGGAAAGCAATCGATGTATTCCAA850.10098130063915223No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)