FastQCFastQC Report
Sat 31 Dec 2022
SRR5848494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83872
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC10541.2566768409004196No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT9921.1827546737886303TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC2640.31476535673407097No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA2420.2885349103395651No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2050.24442006867607782No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACA1430.17049790156428846No Hit
TAGTCCGGGTATAGGGTCAAGTGACTAAGTGCATGTGGTGGATGCCTTGGCG1110.13234452499046168No Hit
GAATTAGGGGTCCTTCAAAGTAGACACTTTGGGAAGTTATTTCTTTATTTTA1100.1311522319725296No Hit
GGCTACGGGAGGTTTTTCTATGAAAGTAAAAGTGGGGGAGTATATTTAAAGA1100.1311522319725296No Hit
AAACTCGGGGTGTGTCCCTGTAACAAGCTTAAATCAAGTGATGCTTACAAAA1090.12995993895459748No Hit
GGTACCGGGAATCAGTTAGTGGAGTGAAAATGCCAAATCCTATGGATTTCTC1090.12995993895459748No Hit
TACCCAGGGAATCACTTCCAAGGCTGTTTAGGTTCTGTTCCACACCTACACT1080.1287676459366654No Hit
GGCGAGGGGCTTCCATCTTACCAAGGAGAGGTGCTTTGCTGAAAGTTAACCA1070.1275753529187333No Hit
AGGTTAGGGGAAAAAATGAATGGAATGCTCCTAAATGATCGCAAAGTATTTG1050.12519076688286912No Hit
TGCAATGGGCTACCATGAATAGATCTGTTTCTTTCTGTCCACAATGATTTGT1040.12399847386493704No Hit
CATAAAGGGAAATAATAAAAGACAAATTATCTTGTCAATTCTCTATTTCTAG1040.12399847386493704No Hit
ACATTGGGGTACAAGAAAACAAAAATCAGTCAAAAGATTTGGCTGTTCATCT1020.12161388782907287No Hit
AGTCCTGGGCAGGTGGGTGAAGGATATGAACAGACACTTCTCAAAAGAAGAC1000.11922930179320869No Hit
GTCACTGGGGCTCAATAAAATGCTGGCAAACTGAATTCAGCGGTATATCAAA970.11565242273941242No Hit
CCATCGAGGGGAGAGCTATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGA970.11565242273941242No Hit
GCTTTAGGGATCATTTCCAAGAATTTTCTTTTGGAGGTGGATTATTTGATGA960.11446012972148034No Hit
AGCCACGGGATCCAACTTGAAGTCAATTGAATATTAAGGCGCTTTACTTCCG950.11326783670354826No Hit
ACACAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA940.11207554368561617No Hit
TGCTAAGGGTGAAATGACACAGGAGCCAACTTGAAGGGACTCCCATTAACCA940.11207554368561617No Hit
TCAGCTGAGCAGGCCAGGCAGATGAGCTCCCAGCCACTGTGATACTTAAGCA940.11207554368561617No Hit
AAACAGGGGGTAAACCCTGAAAGGCAAAGTCAAAAAAATGTTATCAGTATCC930.11088325066768408No Hit
ACAAGTGGGGCCGTGACAAGCCCAACCGGACGGCTGGAGAGGGCGAGAAGGG930.11088325066768408No Hit
ACACCAGGGTAATTGCTGGGGCAAGAAAAATTGTTCAAAAACCAATCTGGAT930.11088325066768408No Hit
ATGAGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC910.10849866463181991No Hit
GGTAGCTTGGAGTCGAAGGGTGGTAGTACCCGAGCTATCAGCTGGTGTAGCC910.10849866463181991No Hit
TACAGGGAGTTACATGTAATTTTTTCGTTATACTTCAGTAAATTTCTGCTTT910.10849866463181991No Hit
TTTGCAGGGGGGAAAGGGCTCTGTGTGGTAACACCTCAGTTGTGTTCTCCTG900.10730637161388781No Hit
TGTGTTGGGGAAGCAGGATAAATAAAATCTCTAATCTAGATACATCAAAGAC900.10730637161388781No Hit
ATTTGAGGGAAGAGAAACTGACCCACAAAATGGAAGCTAATAAAGAGAACCG900.10730637161388781No Hit
TAGCCTGGGCAACAGAGTGAAACTCGTCTCAAAAACCAAAACCAAAACAAAA890.10611407859595573No Hit
CTAGGGGGATAGTCCAACTGTGTCTACTGAATAGTAATCATTCAAAACCTGT880.10492178557802365No Hit
TATCTTGGGATGCAGAAAATAGTGAGATTAAGATCGTCCTTATTGTTTCTAA880.10492178557802365No Hit
TGTGGAGGGCTCCCTGGGTGCCATGCTTCACAAAGTATGTTGTAAGATATGT880.10492178557802365No Hit
TTATAGGGAGAAGCATTTCTAGACAACATGTTTTATTTTCTTATAAAACTCC880.10492178557802365No Hit
GTATACGGGTACTTGAAAAATGCTTTTCTGTGATTGCTAAAGTCTCTGAAAT880.10492178557802365No Hit
ATGGCAGGGTGGACCGGGTTCCAGACCAAGGCTGAAGTGGAGCAGGTGGAGC880.10492178557802365No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACAACTCGTATGC880.10492178557802365No Hit
TAATTTGGGAAGATAATTATATTGCCACTAATAATCAAGATGCTAAATGAGT870.10372949256009156No Hit
GGTCTAGGGAGAAAAATAAATGTTTAAAAAACTATCTCAAACTCAGAATTAC870.10372949256009156No Hit
AGCTATGGGTAATGCAAATACTAGGGGTTATGAATTGTTTCTAAGTCTATTA870.10372949256009156No Hit
GATATAGGCAGAAGAATTTTAAGGTAATGACAGTTTTACACCATCTGTCTTA870.10372949256009156No Hit
TTCAATGGGGAAAAAAAAGCTGGAAAAAGAAGAAGAGGAAATAAGGTAACTC860.10253719954215948No Hit
GCTTGGGGGAGTATTTTGTGGACCAAATTACAATAGGTGGTATTGTACATAA850.10134490652422738No Hit
TACAAGGGAATATAGAAGCATAGTAAACTTTTAAGAAAGATAAATTTTCCTT850.10134490652422738No Hit
GTCCTGGGGTTCATGGTTTATTTTATTCAGTGAGTTATAATTCATTAATATT850.10134490652422738No Hit
AGCAAGGGAGTGTTACTGCAAATCAGTAGACAAAAGGTGTTTTTTTAAAACC850.10134490652422738No Hit
TTTAATGGGAGGAGATCAGAAATGGTTCTATTTCAAGGTCTCTCACAGGGCT840.1001526135062953No Hit
CCAGCCTGGGTGACAGACTGAGACTCCATCTCAAAACAAAACAAAACAAAAC840.1001526135062953No Hit
CTTCTGGGGAAAGAGCTCACAGTTAATTTTACACTAACACCTAAGGCTTTAA840.1001526135062953No Hit
GCAAACCGGGATGAAGGGAACATTTTAAAATCTATTTTTGATTATTACTATG840.1001526135062953No Hit
GAGTGCGGGGAGAGATGAGGATCCAGTTTCATTCTTCTACATGTAGCTTGCC840.1001526135062953No Hit
AAGACGGGGCTTGATTTCAGATGCTATATTAGTTTTCTTTGGTTAAGTAACA840.1001526135062953No Hit
TGTGCTGGGTAGTTCCTTGTTCCTTGACTGGCCTCTGGCTCTCACCACGTAG840.1001526135062953No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)