FastQCFastQC Report
Sat 31 Dec 2022
SRR5848493.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848493.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84143
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC10251.2181643155105No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT9611.1421033241030152TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC2560.3042439656299395No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA2410.28641717076881024No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2190.2602712049724873No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACA1260.14974507683348584No Hit
GCTATAGGGGGAGTTTACAATCCAGTGTCAAGAAATGAGAAGCAAAGAAGTA1120.13310673496309855No Hit
GTCACTGGGGCTCAATAAAATGCTGGCAAACTGAATTCAGCGGTATATCAAA1100.13072982898161462No Hit
CCATCGAGGGGAGAGCTATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGA1090.1295413759908727No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACAACTCGTATGC1090.1295413759908727No Hit
CTTCACGGGGATGTACATTGGTTTGGTCCAGAAAGGACGCACAACTTGAAAA1000.11884529907419511No Hit
TGTGTTGGGGAAGCAGGATAAATAAAATCTCTAATCTAGATACATCAAAGAC1000.11884529907419511No Hit
GGTAGCTTGGAGTCGAAGGGTGGTAGTACCCGAGCTATCAGCTGGTGTAGCC980.11646839309271122No Hit
CATAAAGGGAAATAATAAAAGACAAATTATCTTGTCAATTCTCTATTTCTAG980.11646839309271122No Hit
AAACTCGGGCTGCTTGAGGGAACTGCTAGCCATGACTCAAGACTCAGCTCAA970.11527994010196926No Hit
GTAGAAGGGATAGTACTTTTGGTACATTGTGGCTTCAAAAGGGCCAGTGTAA930.11052612813900145No Hit
GATATAGGCAGAAGAATTTTAAGGTAATGACAGTTTTACACCATCTGTCTTA930.11052612813900145No Hit
TATGGTGGGCTGGCAAATGGGGGCTGAAGCAAATTTGATTTGATTTTGGAAC920.10933767514825952No Hit
TTCCGGGAGCCAATTTAAGTGAGGAAACATATTTCCATTTAAGGGCAAGGAA920.10933767514825952No Hit
CGCTTTGGGCAACTTAGTACATTAAAGCTACGAAAACTCATCCTGGCTGTAG920.10933767514825952No Hit
TAGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC920.10933767514825952No Hit
AGGTTAGGGGAAAAAATGAATGGAATGCTCCTAAATGATCGCAAAGTATTTG910.10814922215751756No Hit
TTATAGGGAGAAGCATTTCTAGACAACATGTTTTATTTTCTTATAAAACTCC910.10814922215751756No Hit
ATCTATGGGACCTCACTGCTGCCTCAGCTTCCTGGGCTCAAGTGATCCTCCC900.1069607691667756No Hit
GTCCTGGGGTTCATGGTTTATTTTATTCAGTGAGTTATAATTCATTAATATT900.1069607691667756No Hit
GTGCTAGGGCCGATCTGAAGCAGGACAGTAATCTCGAAGAAGTACAGTGATT900.1069607691667756No Hit
GTACCAGGGGTACTTTCCAGCAGTCAGTCCCTGGTTCCTAGATGTGTTCTAA900.1069607691667756No Hit
ATTTGAGGGAAGAGAAACTGACCCACAAAATGGAAGCTAATAAAGAGAACCG900.1069607691667756No Hit
GTATTGGGGGGCCCATCCTGTTTTTTAAAGTTTCAGCTGTGAGGTAGGGCCA890.10577231617603366No Hit
GGCTTACGGGTAATCTAGAGATCTATCAATGGTCACATCTCAGTTTTTTTCT890.10577231617603366No Hit
TAGCCTGGGCAACAGAGTGAAACTCGTCTCAAAAACCAAAACCAAAACAAAA890.10577231617603366No Hit
TTGTATGGGTGACAAAGCAGTTGGATTTGGCATGTGTGATGAAAACAATTAG890.10577231617603366No Hit
ACCGCCGGGACAAGGTACCAGGAGGAAATTTTTTAAGGGATCAACTGTATCA890.10577231617603366No Hit
AACTTGGGGCCTCGAAGGTAATGAGAACTACTGGACCGATTACGCTAACCTC890.10577231617603366No Hit
TATTGCGGGATGCTTTATGCTGTTCTGACAAGACTAGATTAATTTGGGGTAT880.10458386318529171No Hit
ATGGCAGGGTGGACCGGGTTCCAGACCAAGGCTGAAGTGGAGCAGGTGGAGC880.10458386318529171No Hit
TCAGCTGAGCAGGCCAGGCAGATGAGCTCCCAGCCACTGTGATACTTAAGCA880.10458386318529171No Hit
TATCTTGGGATGCAGAAAATAGTGAGATTAAGATCGTCCTTATTGTTTCTAA870.10339541019454976No Hit
ATGAGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC870.10339541019454976No Hit
TTTCAAAGGGCCTGTAAATTATTTTTAATATATAGCATGAGAGTGAACTGAG870.10339541019454976No Hit
ACATATGGGGGATCATCTGGCTGAGCAACTGAATTACAAAAGAGAATTACTT870.10339541019454976No Hit
TAGCGGGGGTGCAGCCCCAGAAGGTCGTGGACGCGGCGAGAAAGCACGGGGT870.10339541019454976No Hit
TCCGGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTTCC860.10220695720380779No Hit
AAACTCGGGGTGTGTCCCTGTAACAAGCTTAAATCAAGTGATGCTTACAAAA860.10220695720380779No Hit
TCATTAGGGTTGGCCTTATTTTTTGCCTCTCCCAAACACCCAGTCTTCTCTT860.10220695720380779No Hit
TTGCTGGGGGCTTAACTGTTGTGTATCTTTTTTGTTCTTTACAAGAAGTGCA850.10101850421306585No Hit
AACAATGGGACTTTTATAAGTCTGCAAAAGTACGTAATATTAACAAAGTGTA850.10101850421306585No Hit
GGTACCGGGAATCAGTTAGTGGAGTGAAAATGCCAAATCCTATGGATTTCTC850.10101850421306585No Hit
TGCAATGGGCTACCATGAATAGATCTGTTTCTTTCTGTCCACAATGATTTGT850.10101850421306585No Hit
GCTGTCTCTTTCCTTTCTTAAAACCTTAAGGTCTTTCTAGAGACCTATTCAA850.10101850421306585No Hit
GGGCAAGGGAAGGGCTGAAGATATAGCAGTGCCCAGAACAGAAAAAGATACC850.10101850421306585No Hit
CTATATGGGTCTTAATGTGCTACCTATTACATCATCATATCTGCTCACAGAT850.10101850421306585No Hit
TTCAATGGGGAAAAAAAAGCTGGAAAAAGAAGAAGAGGAAATAAGGTAACTC850.10101850421306585No Hit
TAGTCCGGGTATAGGGTCAAGTGACTAAGTGCATGTGGTGGATGCCTTGGCG850.10101850421306585No Hit
TAAAAAGGGAAGAAGGAATGATAGAATTAGTATATTTGCATTTTATAATCTT850.10101850421306585No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)