FastQCFastQC Report
Sat 31 Dec 2022
SRR5848483.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848483.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202092
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT17480.8649525958474359TruSeq Adapter, Index 11 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC16140.7986461611543257No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC4820.2385052352393959No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA4460.22069156621736635No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2760.1365714625022267No Hit
TAGCCAGGGGAGACAATATTTGTATGTCATATGTCTGATAAGGGACTTATAT2510.12420085901470618No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACA2370.11727332106169466No Hit
TCAGACGGGTAGTAGAAATAATGATGTCTAAAGCTTACCAACAAAAGAACCC2300.11380955208518892No Hit
GTAGAGGGGATTTGGATAGGTTTTCTTTACTTCTTTGAACATATTTAAAATA2300.11380955208518892No Hit
ACTGTTGGGCTAAAATTTTTCATAGAATTTTAAAATCTAATTCTGACTTGTA2270.11232507966668645No Hit
TTACTAGGGGACCGGCGGCCCAGGCACGGAGAGGCGGCGAGACCGCAGAATT2260.11183025552718563No Hit
TCAGCCGGGGCAAGATGTTCTTTGCAAGCCTCATGATAACCTCAAATCAAAA2220.10985095896918234No Hit
AACGCTGGGAGATGGAGTGTTCTTCTTCAACTTTAATTCTGGATATTTTAAA2220.10985095896918234No Hit
TACTACGGGCAGGTGGGCTTACTCACCATACCTGGGGCCACATTACTGTCCC2210.10935613482968153No Hit
TGAAATGGGATTTTCAGCTTTTCAGGCCATATGGTTTCCATCACAACTATTC2210.10935613482968153No Hit
TAGGACGGGGAGCACACAGCGTGGTACACCACAAATGCTCAACCAACAGCAG2200.10886131069018071No Hit
CACTTCGGGGGGTTCAACGTGCCTGGCACAAAAAGTGAGTGCTTGTTAAGTT2150.1063871899926766No Hit
CGTTTTGGGCTTTGCTCTTGTGGTAATAATTAAAAAATGGTCCATCCCTTGT2140.10589236585317578No Hit
CTTGTAGGGCAGAAGACGATCACCTTCTCCTTATTATAGTCGATATAGATCA2130.10539754171367496No Hit
GCTCTTGGGCTTGCATGACAGAGGGAATTCGAACAGTTTCCTTCAAGATAAA2120.10490271757417413No Hit
GCCTTTGGGGTGATTCTATGCAAACAAATTTGGGAGAGGATTTTAAAAATTC2120.10490271757417413No Hit
GGCTGCGGGTAATTCTGTGAGTTCTCCACTTCAGTCTCTTCAGCAGAGAACA2110.10440789343467331No Hit
GTCAGAGGGCGTCTCTGTCCCGGTTCACCTTGCCGAGAGAGGCGCGTCTGCC2110.10440789343467331No Hit
AAGCTGGGGTGGCTTCACTGCTGACTTTTACCAAATATTTAAAAAAGAACTA2100.1039130692951725No Hit
TTTGGCGGGTATATACTAGCAATACATTGAAACTAAAAATAAAATTCCAATA2080.10292342101617086No Hit
AAGCTAGGGGGAAAAATCAGCAATACTGATCTTGTTTTATTTAAAATTTTGA2070.10242859687667002No Hit
GGTAAAGGGCAGGTCTCATACGGTAAACAAGTGTCCTTTCAGCGGTGTATTT2050.10143894859766839No Hit
CGCGCTGGGTGGCTTCATCTATTGGGTTTTGAAAGAATAAACTGAATGAAGA2040.10094412445816757No Hit
TTAGAGTGGGGACTTAGTTGCGTTACACCCTTTCTTGACAAAACCTAACTTG2040.10094412445816757No Hit
TCGTTGGGGATAAAACCCCAGCATCAACCACATTGCAGAGAAAGTGTCATAT2040.10094412445816757No Hit
GGTGCAGGGAGACCCACACATGGAAAACCAGAAAACATTGCTAAGAAAAGTT2030.10044930031866675No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)