FastQCFastQC Report
Sat 31 Dec 2022
SRR5848482.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848482.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202780
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT17440.8600453693658151TruSeq Adapter, Index 11 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC15570.7678272018936779No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC5280.26038070815662295No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA4510.22240852155044877No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC2920.1439984219351021No Hit
TAGCCAGGGGAGACAATATTTGTATGTCATATGTCTGATAAGGGACTTATAT2470.12180688430811717No Hit
ACTGTTGGGCTAAAATTTTTCATAGAATTTTAAAATCTAATTCTGACTTGTA2360.11638228622152086No Hit
TTACTAGGGGACCGGCGGCCCAGGCACGGAGAGGCGGCGAGACCGCAGAATT2340.11539599566032152No Hit
TAATCAGGGGACTGACCTTGATTCTAAGCTCAGCTTTGTTACTTATTAGCAA2260.11145083341552421No Hit
CACTTCGGGGGGTTCAACGTGCCTGGCACAAAAAGTGAGTGCTTGTTAAGTT2250.11095768813492454No Hit
TCAGCCGGGGCAAGATGTTCTTTGCAAGCCTCATGATAACCTCAAATCAAAA2240.11046454285432489No Hit
TTTGGCGGGTATATACTAGCAATACATTGAAACTAAAAATAAAATTCCAATA2240.11046454285432489No Hit
TTAGAGTGGGGACTTAGTTGCGTTACACCCTTTCTTGACAAAACCTAACTTG2220.10947825229312555No Hit
GGTAAAGGGCAGGTCTCATACGGTAAACAAGTGTCCTTTCAGCGGTGTATTT2220.10947825229312555No Hit
ATGACCGGGAAGCTTACAAATCTTGAAACAAGCAACTTTTTAATAAGTTGAC2210.10898510701252588No Hit
GTGCTTGGGGAGAGAAAAGGTGCTGAAAGAGAGGACCATTATTTTATTCTCT2190.10799881645132657No Hit
GGTGCAGGGAGACCCACACATGGAAAACCAGAAAACATTGCTAAGAAAAGTT2170.10701252589012723No Hit
ATTTCTGGGTCAGATTCTCCTTTTTAAGTGTCAATTTCTGTTCACAGACAGT2170.10701252589012723No Hit
TCTCTCGGGGCATTACATCTTCTCAAAGAATTAAATGCCACTAGTAGAATTC2150.10602623532892791No Hit
GGCTGCGGGTAATTCTGTGAGTTCTCCACTTCAGTCTCTTCAGCAGAGAACA2150.10602623532892791No Hit
TGAAATGGGATTTTCAGCTTTTCAGGCCATATGGTTTCCATCACAACTATTC2150.10602623532892791No Hit
TAGGACGGGGAGCACACAGCGTGGTACACCACAAATGCTCAACCAACAGCAG2140.10553309004832823No Hit
GTCACTGGGGTTGAGATTAAATGAAATTTTCTGGCACATAGCAGGTATTATT2140.10553309004832823No Hit
GCTATAGGGAACCCAGTGTGCGTGTACACACACACGAAACAAAAGTTTATAA2140.10553309004832823No Hit
CGCGCTGGGTGGCTTCATCTATTGGGTTTTGAAAGAATAAACTGAATGAAGA2090.10306736364532991No Hit
CAGCCTGGGCGACGGAGCGGGACTCTGTCTCCAAAATAAAAAATAAATTAAA2070.10208107308413059No Hit
CTTGAGGGGCACTTACTGAAGGACAAGAAAAGATCAATAAGAGATCCTTGAG2060.1015879278035309No Hit
TTGTCTGGGCTGGTATTCCCTTTTTGGCTTTTGAATTTAATGGACCACTAAA2060.1015879278035309No Hit
AAGCTAGGGGGAAAAATCAGCAATACTGATCTTGTTTTATTTAAAATTTTGA2060.1015879278035309No Hit
TGCAGCGGGGAGTCCTCGAAGGCTCGGAAGCCCGTCACCATGTCGTGCGAGT2040.10060163724233159No Hit
AAGCTGGGGTGGCTTCACTGCTGACTTTTACCAAATATTTAAAAAAGAACTA2030.10010849196173194No Hit
TGACCTGGCTTGTGCTAGTAAAGTGATTTGTATTTTAATCTCTCACCTCTTT2030.10010849196173194No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)