FastQCFastQC Report
Sat 31 Dec 2022
SRR5848481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203476
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGCCGTCT17260.8482572883288446TruSeq Adapter, Index 11 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACATCTCGTATGC14180.6968880850812873No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTATACAC5020.24671214295543453No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAACACTGTCTCTTA4550.22361359570661896No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGGCAAC3200.15726670467278697No Hit
TTTGGCGGGTATATACTAGCAATACATTGAAACTAAAAATAAAATTCCAATA2490.12237315457351236No Hit
CGCGCTGGGTGGCTTCATCTATTGGGTTTTGAAAGAATAAACTGAATGAAGA2450.12040732076510252No Hit
GGTAAAGGGCAGGTCTCATACGGTAAACAAGTGTCCTTTCAGCGGTGTATTT2410.11844148695669268No Hit
TAGCCAGGGGAGACAATATTTGTATGTCATATGTCTGATAAGGGACTTATAT2410.11844148695669268No Hit
GGACAGGGGAAGTAGCCCAGTATTGCCTTAAATAAAATCACATTTCATTTCT2270.11156106862725826No Hit
ATTTCTGGGTCAGATTCTCCTTTTTAAGTGTCAATTTCTGTTCACAGACAGT2250.11057815172305333No Hit
TAGGACGGGGAGCACACAGCGTGGTACACCACAAATGCTCAACCAACAGCAG2240.11008669327095089No Hit
TCACAGCGGGCAATAGCAAGTTGTATGGACAATGTAACAATATTAATTTTTC2170.10664648410623366No Hit
TTAGAGTGGGGACTTAGTTGCGTTACACCCTTTCTTGACAAAACCTAACTTG2170.10664648410623366No Hit
TACTACGGGCAGGTGGGCTTACTCACCATACCTGGGGCCACATTACTGTCCC2150.10566356720202875No Hit
TGACCTGGCTTGTGCTAGTAAAGTGATTTGTATTTTAATCTCTCACCTCTTT2120.10418919184572135No Hit
GTAGAGGGGATTTGGATAGGTTTTCTTTACTTCTTTGAACATATTTAAAATA2120.10418919184572135No Hit
TCTACTGGGATTTAGCAACTGTCAGTGACAATACTCATCTAATTTCTCTTTA2110.10369773339361889No Hit
TCAGACGGGTAGTAGAAATAATGATGTCTAAAGCTTACCAACAAAAGAACCC2100.10320627494151645No Hit
GCACCTCTCAGCCTTCATAGAATTGAAGAGAGTTCGGGCCTTGCTCATGATT2090.10271481648941398No Hit
CTTGAGGGGAATAGAGGATAACCATTCTCTGAATAACCTCCTTAGTCTTTTC2080.10222335803731153No Hit
ATGACCGGGAAGCTTACAAATCTTGAAACAAGCAACTTTTTAATAAGTTGAC2070.10173189958520906No Hit
CACTTCGGGGGGTTCAACGTGCCTGGCACAAAAAGTGAGTGCTTGTTAAGTT2070.10173189958520906No Hit
TCAGCCGGGGCAAGATGTTCTTTGCAAGCCTCATGATAACCTCAAATCAAAA2060.10124044113310662No Hit
CGTTTGGGCGATCTTGTAGCGGGACAGTTACTGGCTACTTGCATTTGTCAGT2050.10074898268100416No Hit
GCGTATGGGGTTCTCCTACTGACCTTTGATCTAGTTTGACCTTTGAAATTTG2050.10074898268100416No Hit
AAGCTGGGGTGGCTTCACTGCTGACTTTTACCAAATATTTAAAAAAGAACTA2040.10025752422890169No Hit
ACTGTTGGGCTAAAATTTTTCATAGAATTTTAAAATCTAATTCTGACTTGTA2040.10025752422890169No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)