FastQCFastQC Report
Sat 31 Dec 2022
SRR5848421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251825
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAAACAGTATCTCGTATGC17240.6846024024620272No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACAGTATCTCGTATGCCGTCT16780.6663357490320659No Hit
TGTTAGGGGAAATTATTTTAGGAAATGGAAGATAAAGTGACTAGTCCAGAGA2760.1095999205797677No Hit
GATACAGGGAGGGAAATTTTGTCAATACACTCAAAGCAGGAAAATGTATTTC2690.1068202124491214No Hit
TTAAACGGGTATAGCATGTGGTTATGATAGAAGAATATCCTTATGTTAGGAA2610.10364340315695422No Hit
TAATATGGGCAAGTAAAATGATTTAATTTCAAACGAAAACATTTCCCACAAC2550.10126079618782884No Hit
TATCATGGGGGACTGACTTTTTAGAAAAGCCATTTAATTTACCCACTAATAT2540.10086369502630795No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)