FastQCFastQC Report
Sat 31 Dec 2022
SRR5848420.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848420.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176448
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGCCGTCT7190.4074854914762423TruSeq Adapter, Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGC6740.3819822270583968No Hit
TGAGATGGGTGGAAAACATGCTTCTCAATTCAAATATATCGTAGTTTTATTT2320.13148349655422562No Hit
CGGGTTGGGAGACAGATGTTTATTACGCAGTAAATTCACACAATTCCATAAG2200.12468262604280013No Hit
CAGCTCGGGGGTGGGCCCGGCAGTCCTGACCTGGCAAGGCATTACAAATCTA2120.1201487123685165No Hit
GGCTATGGGAAGAATTGGCATAAATCTAAGAAGAAGGCCTTTACCAAGTACT2110.11958197315923105No Hit
GACCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCA2100.11901523394994559No Hit
GATGCCGGGCAAGTCATCCCAAATATGGTTCCATCCCTAAACTTATACTTGC2100.11901523394994559No Hit
TAACTTGGGATTCTGAGCTGACCCAGTGGGGACATGCAAACATTTTCCATAA2080.11788175553137467No Hit
TATAGTGGGAGGGGTGGGGTGGGGGGCGGAGTCCCGCCCAGCATAGCTGCAG2070.11731501632208922No Hit
TTCAAAGGGGGGACATTTTTAATATATTTTAGAGACCTCTGAAGATTCCTCG2050.1161815379035183No Hit
GCATATGGGACATGCTAATATTGCTAGTTCTTGATTTTTCTTCCATTTTAGG2040.11561479869423286No Hit
GTTTAGGGGGCCATGGATGTGGTGGTCCAGTTTGCCATCCACCGCCTGGGCT2020.11448132027566196No Hit
TTAACAGGGGAAAGACGCAAGTCCCATGAAGCTGAGGTCTTGAAGCAGCTGG2020.11448132027566196No Hit
GTGAATGGGAAGGTCAGCATGAGGACAGACCACAGAGCTGTCACTTTTGCTC2010.11391458106637649No Hit
CCTTCTGGGAAGTGCGAAATACTTTGTCTACCATGTTAGGAAAGTCTGCCAC2000.11334784185709104No Hit
AGACTTGGGATCTAGATAGATCGATCTAAGGTACTGATCCTACAAATTCCCC2000.11334784185709104No Hit
CACGGAGGGACTGAAAATGAAGAATGATAATGAGAAGAGAGCCTAGTTTTAC1990.1127811026478056No Hit
GAGCCGGGGCAGGGAGAAACAAAATACATCCCCAACAACCAGAAAACAAATT1980.11221436343852015No Hit
TACAATGGGGGCTCTCGCTCGGTCTTTCTGCCGCCATCTTGGTTCCGCGTTC1980.11221436343852015No Hit
AGATCCGGGATGTTGAAATAATTCCATATTTTGCTTTACATTTGGATGAAAC1960.11108088501994923No Hit
GCAATGGGGGCTTCATGATTATCTCTGGTTGCTGTCTTAGACTGAAGCATGT1960.11108088501994923No Hit
CATCGCGGGGGACAGACATTTGTTTTTCATGTGGTATGTCGGGAGTATGAAC1960.11108088501994923No Hit
TCTGTTGGGATCTGAGGAACCAGTGAGATGGCATCAAACTTACCAAAATTTG1960.11108088501994923No Hit
GTTGCAGGGGATGCCTCTAGCTTTGTTCTTTTTGCTTGGGACTGCTTTAGCT1950.11051414581066377No Hit
AACTTAGGGATTTCCTCATCTGTAAAATGGGGATGATAATAGTAGCTACTTC1940.1099474066013783No Hit
CGCACAGGGGAGATAATTATTTTACTGCCATGAGTAAGTATAGAAATTTCAA1930.10938066739209285No Hit
GTTTACGGGGTGATGAACAGGGAAGGGACACAAGGAAGCTTTGTGAGAACTC1900.10768044976423648No Hit
CAAGCTGGGCAGCTCAAGTCCAAAAAGATGCACGGATTGGAGAAGCAGAGGC1890.10711371055495103No Hit
AAGCACGGGTGGAACTGGAGACATCGCGTACTTCCACCCTTGCAGGCTCAGA1890.10711371055495103No Hit
GCCAAAGGGATTGCACAACAAACACAGATACCTACAGACTCCGTTTTCATTT1890.10711371055495103No Hit
TCATAGGGGAAGCCACGGGGAGATCAACTTACATGAATTTTCAAAAGAAACA1880.10654697134566558No Hit
CGCAGGGGATGTCTTCATTATTTAATACCTTTGCACATAGCATATTGTGAAA1880.10654697134566558No Hit
TAGCCTGGGAGGGTGACTATAATTAAAAACAATACCAGAAAATAATAAGTGT1870.10598023213638014No Hit
GTGACTGGGGCGAGACAAGCTCACCGAAATGGCCGCGTCCAGTCAAGGAAAC1870.10598023213638014No Hit
GCAAAAGGGTGAAGGCAGCCAGGAGGGAGGCTGTACCCTGCAGAGCCACAGA1860.10541349292709466No Hit
TACTTAGGGCAAAATAGCTGTTTTGAAACTGTTTTAATTGCTTGGGTAGCAA1850.10484675371780922No Hit
GATACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1850.10484675371780922No Hit
TGTATAGGGACTATAAATATCATGCAGACTGATTTCATAGTATGTTCATGAC1850.10484675371780922No Hit
GCACAAGGGGGGTCAGTTTGTTGCACATTGATGAATAACAAGTGACACCCAA1830.1037132752992383No Hit
ATAAATGGGGTGCAGAAAGACCTTTGAATGAACAGATTGCTGAAGCAGAAGA1820.10314653608995285No Hit
AGCTGTGGGCTAGGAAGGGATGCACTGTAAAACAAAAGTATTCTTGTAACTC1820.10314653608995285No Hit
GCTCTGGGGGGAGAAGTCCCGGGGGCGTTCCATTGAGGTTTAACTGCGTGTG1820.10314653608995285No Hit
TCACCTGGGCAGCAGCTAGCTTAACTGAGATCATTGGTAGCCAGGGGTTGCT1810.1025797968806674No Hit
ATACGAGGGCTCTTTTCCGGTTAGCGCGGCGTGAGAAGCCATGAGCAGCAAA1810.1025797968806674No Hit
GCACTCGGGGGGTGGAGGAGGTGCTGGGAGCAGCCGGGCAGCCGCTTCCCGC1810.1025797968806674No Hit
GCGCAGGGGGGAACTTAATTAAAGGAGGTTTGGCTTGTTTATTTGTTTTTGT1800.10201305767138194No Hit
GCCTCAGGGGCATTCATTTAGACAAGACACCTGAAGTGTGTCTTGTCTATCG1800.10201305767138194No Hit
GAAATGGGGCGCCCTTTCCAAGAATTTCAGTCTTAGTCCTGCTACGTTAACT1800.10201305767138194No Hit
GGATCAGGGAGTGCTTGAGCTGCAGAAACTGAGACCAGACCTCTGGCCTGGC1800.10201305767138194No Hit
GGTGACGGGGTTCCTTAATGGGTTTTTCAAAAGGAAAGTAACAACCCTAGCT1800.10201305767138194No Hit
CTTTAAGGGAGAAACATTAGAGGTAGAAAGAGCAAGTGATGAAAATTACTAG1790.10144631846209648No Hit
TTTCACGGGATTGACTATGGGAGATAACAACTGATAGACCTGGAGAAATTAC1780.10087957925281102No Hit
GTGCGGGGATGCCTGAAAACATGAGTGTATTGAGTAGATGAAGACTAAATCA1780.10087957925281102No Hit
CTGCAAGGGATCTGGGACTTGTGAGGACTCACATAAATGCTGTTGTGGCAAA1770.10031284004352557No Hit
CGTGAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1770.10031284004352557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)