FastQCFastQC Report
Sat 31 Dec 2022
SRR5848419.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848419.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174097
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGCCGTCT7210.4141369466446866TruSeq Adapter, Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGC6590.37852461558786193No Hit
TTAACAGGGGAAAGACGCAAGTCCCATGAAGCTGAGGTCTTGAAGCAGCTGG2430.13957736204529658No Hit
GCCTCAGGGGCATTCATTTAGACAAGACACCTGAAGTGTGTCTTGTCTATCG2260.12981269062648984No Hit
GACCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCA2070.11889923433488227No Hit
TATATTGGGAGGATGCAAAGCTGGTTTGACATATGCAAATCAATTAGTGTGA2060.11832484189848189No Hit
GCACTCGGGGGGTGGAGGAGGTGCTGGGAGCAGCCGGGCAGCCGCTTCCCGC2060.11832484189848189No Hit
TATAGTGGGAGGGGTGGGGTGGGGGGCGGAGTCCCGCCCAGCATAGCTGCAG2050.11775044946208148No Hit
TCGCCCGGGCAGTCTTGTTAAATTGACATAAATGGTAAACTTCAACATTTTC2040.1171760570256811No Hit
AGACTTGGGATCTAGATAGATCGATCTAAGGTACTGATCCTACAAATTCCCC2010.1154528797164799No Hit
GGATCAGGGAGTGCTTGAGCTGCAGAAACTGAGACCAGACCTCTGGCCTGGC2010.1154528797164799No Hit
TCTGTTGGGATCTGAGGAACCAGTGAGATGGCATCAAACTTACCAAAATTTG2000.11487848728007949No Hit
GCACTGGGCCTCTCAAACTAGTAATCATAGTCAGTTGGTGATTCATAGGTAT1990.11430409484367911No Hit
GCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGG1970.1131553099708783No Hit
CTTTCAGGGGTCTCACTGTCGCCCAGGCTGCAGTGCAGTGGTGCCATCACGC1970.1131553099708783No Hit
CCTTCTGGGAAGTGCGAAATACTTTGTCTACCATGTTAGGAAAGTCTGCCAC1960.11258091753447791No Hit
TTCAAAGGGGGGACATTTTTAATATATTTTAGAGACCTCTGAAGATTCCTCG1960.11258091753447791No Hit
CGCTTAGGGATGGCTTCCTCAAGAACCCAGTTTCTACTATGTTTCTGAAAGA1940.1114321326616771No Hit
GCATATGGGACATGCTAATATTGCTAGTTCTTGATTTTTCTTCCATTTTAGG1940.1114321326616771No Hit
TAAAATGGGAGGTATGTTGTGTGTTCTGTGTTCTTGCAGCCTATACGATGGG1940.1114321326616771No Hit
GCGCAGGGGGGAACTTAATTAAAGGAGGTTTGGCTTGTTTATTTGTTTTTGT1930.1108577402252767No Hit
AGGTCTGGGGATCTAGTTATGGGCATATGACTTACTAGACTTAATTTATTCC1930.1108577402252767No Hit
GAGCCGGGGCAGGGAGAAACAAAATACATCCCCAACAACCAGAAAACAAATT1920.11028334778887632No Hit
TTTCACGGGATTGACTATGGGAGATAACAACTGATAGACCTGGAGAAATTAC1910.1097089553524759No Hit
GAAGCCGGGGTGGAGGCTCCTGTGGTAGTCCTCCCAAGCTGTGCTGGGAAGC1910.1097089553524759No Hit
TCATAGGGGAAGCCACGGGGAGATCAACTTACATGAATTTTCAAAAGAAACA1900.10913456291607553No Hit
GTCTCAGGGAGAGTCTTCAGCTTTGTTTTCAAATTTATAAGTACAGAAGTGC1890.10856017047967512No Hit
GTGACTGGGGCGAGACAAGCTCACCGAAATGGCCGCGTCCAGTCAAGGAAAC1890.10856017047967512No Hit
AATCAAGGGGTTGCAAGAGACATTTAGGTTGAGGTCAGTGAGTTTTTTTTTT1890.10856017047967512No Hit
AGTAAAGGGGAGATGGAAGGGCGAGAACGTGTCCACGCACGAGGTGGAGGGC1880.10798577804327474No Hit
CGCAGGGGATGTCTTCATTATTTAATACCTTTGCACATAGCATATTGTGAAA1880.10798577804327474No Hit
TACTTAGGGCAAAATAGCTGTTTTGAAACTGTTTTAATTGCTTGGGTAGCAA1880.10798577804327474No Hit
TAGTACGGGAACTGGACTTGGGCTTTTTCTTCTATTTGCTGGGTAGAAAAGT1860.10683699317047395No Hit
TCAACAGGGACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCG1860.10683699317047395No Hit
TCAACAGGGTGAAGTGGTGAAGTACCTGCTTCGGATGGGAGCGGAGATCGAT1860.10683699317047395No Hit
TAGCCTGGGAGGGTGACTATAATTAAAAACAATACCAGAAAATAATAAGTGT1850.10626260073407354No Hit
GTGAATGGGAAGGTCAGCATGAGGACAGACCACAGAGCTGTCACTTTTGCTC1850.10626260073407354No Hit
TGACTAGGGCAGCTTTTGAAAAAGAAACTTTAATGCTCCCTCTTATGTAAGT1830.10511381586127275No Hit
CAAGCTGGGCAGCTCAAGTCCAAAAAGATGCACGGATTGGAGAAGCAGAGGC1830.10511381586127275No Hit
TTTCTTGGGTGGACAACATAGAGACAAATGCAGAAGCTCAGAAGTCAGGGGC1830.10511381586127275No Hit
GTTTCTGGGAAGGGAAGAAGTTTTCTAAATTGTGAAAGTCTGGTTCTTAATT1830.10511381586127275No Hit
AAGCACGGGTGGAACTGGAGACATCGCGTACTTCCACCCTTGCAGGCTCAGA1820.10453942342487234No Hit
TCAACTGGAGAAATTAGAAAAAAGAACAAAATAAAGCTAAGGAAAGGAGAAA1820.10453942342487234No Hit
CTGCAAGGGATCTGGGACTTGTGAGGACTCACATAAATGCTGTTGTGGCAAA1810.10396503098847194No Hit
TAAAGAGGGAACGTCAGTTTATGCACCATGCGAGGCTGGCTGCCTGAGAATC1800.10339063855207153No Hit
GTTGCAGGGGATGCCTCTAGCTTTGTTCTTTTTGCTTGGGACTGCTTTAGCT1800.10339063855207153No Hit
GAAATGGGGCGCCCTTTCCAAGAATTTCAGTCTTAGTCCTGCTACGTTAACT1800.10339063855207153No Hit
TTTAAAGGGGCACGCCTGCTAGGATCAGCGGTGGTGGTTCCGCGATGGTAGG1800.10339063855207153No Hit
ATGTGAGGGATATTGTGCTTTTTGGAGGTTCTAATCATTTCAGCAGTAGCGT1800.10339063855207153No Hit
GCTCAAGGGCTCCCCCAGCCACGCAGCACGGGTGCCAGTGTGCATGCGTGTG1790.10281624611567115No Hit
CAGCTCGGGGGTGGGCCCGGCAGTCCTGACCTGGCAAGGCATTACAAATCTA1790.10281624611567115No Hit
GCAAAAGGGTGAAGGCAGCCAGGAGGGAGGCTGTACCCTGCAGAGCCACAGA1780.10224185367927074No Hit
TACAATGGGGGCTCTCGCTCGGTCTTTCTGCCGCCATCTTGGTTCCGCGTTC1780.10224185367927074No Hit
CGTGAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1770.10166746124287036No Hit
ACCAAGGGGAGTGGTGTTATTGGTGGCTTGCCAAGATGAGACTAGAGATTTT1760.10109306880646995No Hit
AATGCTGGGTAAGAAATTCACTGAAAGTTAAGTAATGCTGTTGGAAAGGAAA1760.10109306880646995No Hit
GGCTGGGATCCACCTCTCTGTAATTCATCCTCTATGCAACAGAGAAAGGGGT1760.10109306880646995No Hit
AAAAAGGGGAGGTGAGGGAGGAAGGGATAGCCAGCGCGAAGGAAGTGCTGGA1760.10109306880646995No Hit
TTTGACGGGGTGACTTCAGATTATGCAGTGCATCCAATGAGCCCTGTAGGCA1760.10109306880646995No Hit
CCCACAGGGCTGCTCCTCTTTTACGTAATCCCATGCTTATTGATTCTCTAGA1750.10051867637006955No Hit
TGAGATGGGTGGAAAACATGCTTCTCAATTCAAATATATCGTAGTTTTATTT1750.10051867637006955No Hit
TTGTTGGGGTGGGTCAGAAAAACCACTGGCAGAAGATCCGGACCATGGTCAA1750.10051867637006955No Hit
GGCTATGGGAAGAATTGGCATAAATCTAAGAAGAAGGCCTTTACCAAGTACT1750.10051867637006955No Hit
ATCTGAGGGCTGATAATTAACCCAGATTAGTTTATTTTGAGCCTCTGAATTT1750.10051867637006955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)