FastQCFastQC Report
Sat 31 Dec 2022
SRR5848418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174424
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGCCGTCT7210.41336054671375505TruSeq Adapter, Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGC6800.3898546071641517No Hit
TTAACAGGGGAAAGACGCAAGTCCCATGAAGCTGAGGTCTTGAAGCAGCTGG2180.12498280053203689No Hit
ATCTGAGGGCTGATAATTAACCCAGATTAGTTTATTTTGAGCCTCTGAATTT2150.12326285373572443No Hit
GCCTCAGGGGCATTCATTTAGACAAGACACCTGAAGTGTGTCTTGTCTATCG2090.11982296014309958No Hit
TAGCCTGGGAGGGTGACTATAATTAAAAACAATACCAGAAAATAATAAGTGT2070.11867632894555795No Hit
CAGCTCGGGGGTGGGCCCGGCAGTCCTGACCTGGCAAGGCATTACAAATCTA2060.11810301334678713No Hit
TACTTAGGGCAAAATAGCTGTTTTGAAACTGTTTTAATTGCTTGGGTAGCAA2010.1152364353529331No Hit
GGCTATGGGAAGAATTGGCATAAATCTAAGAAGAAGGCCTTTACCAAGTACT1980.11351648855662064No Hit
TCAACTGGAGAAATTAGAAAAAAGAACAAAATAAAGCTAAGGAAAGGAGAAA1970.11294317295784982No Hit
GTTTCTGGGAAGGGAAGAAGTTTTCTAAATTGTGAAAGTCTGGTTCTTAATT1970.11294317295784982No Hit
TATATTGGGAGGATGCAAAGCTGGTTTGACATATGCAAATCAATTAGTGTGA1960.11236985735907903No Hit
TGAGATGGGTGGAAAACATGCTTCTCAATTCAAATATATCGTAGTTTTATTT1910.10950327936522497No Hit
CACGGAGGGACTGAAAATGAAGAATGATAATGAGAAGAGAGCCTAGTTTTAC1910.10950327936522497No Hit
GGATCAGGGAGTGCTTGAGCTGCAGAAACTGAGACCAGACCTCTGGCCTGGC1900.10892996376645415No Hit
GTGCGGGGATGCCTGAAAACATGAGTGTATTGAGTAGATGAAGACTAAATCA1890.10835664816768334No Hit
GCATATGGGACATGCTAATATTGCTAGTTCTTGATTTTTCTTCCATTTTAGG1870.10721001697014174No Hit
TAGTACGGGAACTGGACTTGGGCTTTTTCTTCTATTTGCTGGGTAGAAAAGT1860.1066367013713709No Hit
AGACTTGGGATCTAGATAGATCGATCTAAGGTACTGATCCTACAAATTCCCC1850.10606338577260009No Hit
TCTGTTGGGATCTGAGGAACCAGTGAGATGGCATCAAACTTACCAAAATTTG1850.10606338577260009No Hit
TGCTTTGGGGAGATTTCATATTCAGTGAGGTGGGTACCAAATGCATATTAGT1850.10606338577260009No Hit
GAGAAAGGGATACGATATGATGGGTAAATGTACATATCCTGTCCTCAAAGAA1840.10549007017382928No Hit
AGGTCTGGGGATCTAGTTATGGGCATATGACTTACTAGACTTAATTTATTCC1830.10491675457505849No Hit
AGCTGTGGGCTAGGAAGGGATGCACTGTAAAACAAAAGTATTCTTGTAACTC1830.10491675457505849No Hit
GGTGACGGGGTTCCTTAATGGGTTTTTCAAAAGGAAAGTAACAACCCTAGCT1830.10491675457505849No Hit
TCACCTGGGCAGCAGCTAGCTTAACTGAGATCATTGGTAGCCAGGGGTTGCT1820.10434343897628767No Hit
GCACAAGGGGGGTCAGTTTGTTGCACATTGATGAATAACAAGTGACACCCAA1820.10434343897628767No Hit
TTCAAAGGGGGGACATTTTTAATATATTTTAGAGACCTCTGAAGATTCCTCG1820.10434343897628767No Hit
CTTTCAGGGGTCTCACTGTCGCCCAGGCTGCAGTGCAGTGGTGCCATCACGC1820.10434343897628767No Hit
GTGACTGGGGCGAGACAAGCTCACCGAAATGGCCGCGTCCAGTCAAGGAAAC1820.10434343897628767No Hit
GCCAATGGGTAATGGCTAAATCTTTAAAAGCCAATGCAACCCACCCAATTGA1820.10434343897628767No Hit
GCTCAAGGGCTCCCCCAGCCACGCAGCACGGGTGCCAGTGTGCATGCGTGTG1810.10377012337751686No Hit
TTTCACGGGATTGACTATGGGAGATAACAACTGATAGACCTGGAGAAATTAC1810.10377012337751686No Hit
GCACTGGGCCTCTCAAACTAGTAATCATAGTCAGTTGGTGATTCATAGGTAT1810.10377012337751686No Hit
CGCAGGGGATGTCTTCATTATTTAATACCTTTGCACATAGCATATTGTGAAA1810.10377012337751686No Hit
GAGCCGGGGCAGGGAGAAACAAAATACATCCCCAACAACCAGAAAACAAATT1810.10377012337751686No Hit
CTTGGTGGGTATAAAAGAAATTATCCAACACAGTAATATTGGTACCTGTCAT1810.10377012337751686No Hit
ATCTCCGGGGAGTCGTGGTTTGTGCGCGGCCAGGCGCTGGAGCCTCCGCTGC1800.10319680777874604No Hit
GCCAAAGGGATTGCACAACAAACACAGATACCTACAGACTCCGTTTTCATTT1800.10319680777874604No Hit
TATAGCCGGGATGAGAAGCTTCTGTCGGGCAACCTGGCCCTGCGCAGTCTCT1800.10319680777874604No Hit
AAATTGGGGAAGAAATGTATGCACTGTTTGAAATGTAAATTATTCTTAGAAC1800.10319680777874604No Hit
TTTACAGGGGAGTTAAGGAAATCATTCAGAAGGCTGAATCATCTTCATGCAA1790.10262349217997524No Hit
GACCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCA1780.10205017658120442No Hit
CGCACAGGGGAGATAATTATTTTACTGCCATGAGTAAGTATAGAAATTTCAA1780.10205017658120442No Hit
ATAGTCTGGGTCCACTTGGCTGTACATAATAATGATCCATCCTGCTTTGCTG1770.10147686098243361No Hit
ATGTGAGGGATATTGTGCTTTTTGGAGGTTCTAATCATTTCAGCAGTAGCGT1770.10147686098243361No Hit
TGTATAGGGACTATAAATATCATGCAGACTGATTTCATAGTATGTTCATGAC1750.10033022978489199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)