FastQCFastQC Report
Sat 31 Dec 2022
SRR5848401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147037
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGC6170.4196222719451566No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGCCGTCT5320.36181369315206374TruSeq Adapter, Index 5 (95% over 21bp)
TCTAACGGGGCTCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAA2010.13670028632249026No Hit
AAGCATGGGGATGGAGGTTCCACAACAAACAAAAAATAGAATTATCGTATGA1990.13534008446853513No Hit
TCGATAGGGGATTTATGCATTTATGCATGAGAACTAAATAGATTTTTAGATT1980.13465998354155756No Hit
CAACCCGGGCACCCTCGGCCCTCCCTGCAGCCTTAACATTCTCTTCCCCTGC1920.13057937797969218No Hit
GCCAGGGCGATCTTTGCAAGGGTCAGCCGAGCTGGACGAGCAGGACATCTTG1920.13057937797969218No Hit
CTCGTTGGGAGATGATGATAATGGAGGAATACTCGTGGACAAGATGAGTTAA1900.12921917612573705No Hit
GACTTAGGGCAAACAGAAATAGTTTTATTACTTGCTTTGTAATTTCCTTTTA1890.1285390751987595No Hit
GGAGCTCCTCATGTACTCCCATGACAACATCATCTGTGGCATCACCTCTGTT1890.1285390751987595No Hit
AGGTATGGGAAGCTAGCGGAAGACAAGAAATAACTAAGATCAGAGCAGAACT1870.12717887334480438No Hit
ATGATTGGGGAATCCAATAAAGAGAGGCAGCTTGGAAGTAAGTAAATTTTTG1870.12717887334480438No Hit
AATACAGGGGAAGTAGATTATTCAAACAAACAAGGCGAAGAGCAACCTTGGG1870.12717887334480438No Hit
CTAAATGGGGTGGTGAGAAAGTTCTTGAGGATGGTAAGGAGCTCCACATTTT1870.12717887334480438No Hit
TGTGTTGGGAATGCCAGCCTCTGTGCCCGACCTCGGAAAACTGGCCATGCCC1860.1264987724178268No Hit
TGCTCCGGAGTTGGGAAGGTGGAGAGAAGTGGTGGGGGGCAGGAAAAGTTTT1860.1264987724178268No Hit
AGAAAAGGGATCGCGGCTAGAGAGATGTCGCTGCTGCGGTCGCTGCGCGTGT1860.1264987724178268No Hit
CTTGTTGGGGCACCCACTGCTGCACACTAAACTGTCTGCCACCGGCTCCCTC1850.12581867149084924No Hit
AGCGTAGGGATAGCATGCAAAAAAGAAACTATATTTTCTGTCCTGTTCTTTG1850.12581867149084924No Hit
TGTCAGGGGTGCTCCTGAAATCAACCAAACAACCAAACAGACTCTCTAGTCT1850.12581867149084924No Hit
TGATTTGGGCTCTTTGAAGTGGAAAGAACTGATGCATTAAACATTCCTTAAA1850.12581867149084924No Hit
CACCATGGGGGGGTAAGGATTCGGCGGGCAAGCGGGTGTAATCAGCAGCCAT1830.12445846963689412No Hit
AGTTCCGGGTCTCATGGATTTGTTAGGAGTTCTCTTGTATTTTCTGTATTTT1820.12377836870991654No Hit
CACATAGGGATTCAAGTGCGCAGGTAATTACTTGGTTGAAACAATAGTGTTT1820.12377836870991654No Hit
CACGTGGGGATGCAGCAAAGACCTAGTATTAAGGAAGTTTCCCACAAAACAA1820.12377836870991654No Hit
CCGGCTGGGAATTCCACCTGAGAACAAGTCCCGCAAACCGGGGACGGAAGGA1790.12173806592898385No Hit
AAAGTTGGGTCCAGCCCAGTGTCAAGTCCCTGCTGCAGGTGCTGGGCTCTGT1790.12173806592898385No Hit
TTAACTGGGGAGGCAGGAGAATTGCTTGAATCCGAGACATGGAGGTTGCAGT1780.1210579650020063No Hit
CTGTACGGGTCAATTATGAAGAAATAGAAAATCTTAACAAACCATTAATGAC1780.1210579650020063No Hit
CATCTAGGGTAAGAACTTTGGGTATATAGTCAAATTCCTCACATTTTTAGAA1770.12037786407502875No Hit
TTTTGAGGGAAGTTTTCATTGGTAATGGATTTATTCCAGACAGGACAGGTTA1760.11969776314805117No Hit
GCTTTGGGGATATTTTAAATAGACTAACTTTTAAGTGAATGCTTTGTACTTT1760.11969776314805117No Hit
CATAATGGGATTTTTGTGCTGCTGTACAATGTTTATGTTTTAAGATATCTTA1750.11901766222107361No Hit
TATAGTGGGCAACCAGAATAACTCCTCTTAGAAGAATCATCATCCAGCACTA1740.11833756129409606No Hit
TGGCATGGGAAGAAACAACTGTCAGGGCAACCAGTATATTTGAGAGGACATG1740.11833756129409606No Hit
TGGTCAGGGCATTTTAAGGATGGGCATAAGCAAACTATATTTTAATAATTTA1740.11833756129409606No Hit
TGTAACGGGCTCTCATGTTGGACACGGGCCTGCCCTGTTTTCGCGGCCAGAC1740.11833756129409606No Hit
CAAATGGGGGATCTTTCTGCGGCGCGCGGACACCCGCCGGTGGAAGAAGAAA1730.11765746036711848No Hit
TCAAAAGGGGATCTGTCTAATGTTGACAGTGGGGTGTTAAAGTCTTCCATTA1720.11697735944014093No Hit
AAAAGAGGGGATTCCCGGAGGGCAGTTGGGGAGCGCAGATCCCGAAGCAGCG1710.11629725851316335No Hit
TGTCTGGGGGCTCCAATGCATTTGCATCAGAATTCAGAGTTCAGTCTGCTGA1700.11561715758618579No Hit
TTTGGTGGGAGTCTTCAGTGATCATGATTCACTAACTTTCTGTGCTAGATAT1690.11493705665920823No Hit
ATTTTTGGGTAAGACATTGTGTAAAAAGCAATCTGTAAAAACATCTCCAGGC1690.11493705665920823No Hit
TGTCAAGGGATATTGACAAATTTATTGAAGACACAGAAATTTTACTAATGCT1690.11493705665920823No Hit
GGGAGTGTTCTGATTGGTTCTAAGGTAAACTTTTGTTTTTGAGAAACAACAG1690.11493705665920823No Hit
TATATTGGGGCAGTGGCAGAAGTGGATTTTTGGGGGTACGTTTTCAGATGTG1670.11357685480525309No Hit
ACCACTGGGCTCTAAAAGGTTAATTTCTGTCAAATGCAGTAGATGATGAAAG1670.11357685480525309No Hit
TTATAAGGGGATTCCCTCAACCAGAAGGTAAGGTAGAGATTCCTGGCCCTGG1670.11357685480525309No Hit
TAAACAGGGTGTCTTGGCGTATTGCCAATTCACCACTTTCTACTTTGTGCTG1670.11357685480525309No Hit
TTTAAAGGGATGTACAAAGCATTATTGAAACAGCGATGATATAAAAAGAAAT1670.11357685480525309No Hit
CAGCTGGGGAGAGGAGGCTGGCTCAGAACACCTAGAGGAGGGGGCCGGGGAT1660.11289675387827554No Hit
CGTTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1660.11289675387827554No Hit
GAGTCTGGGAGGATGGTCTCGATCTCCTAACGTCGTGATCCTCCCGCCTCGG1660.11289675387827554No Hit
GGTCAGGGGAGGGTGACAGACTTCACCGGAGATCAGAGATCAGTTGATGCAG1660.11289675387827554No Hit
AGCAAAGGGAGCAACAAGAACGGGATAAAGATTTGATAGAGTCACTTTCTGA1660.11289675387827554No Hit
TAAACTGGGCGCCTCCTACGTTTACCTGTCCATGTCTTACTACTTTGACCGC1660.11289675387827554No Hit
ATGATCGGGCATTGCCTGCACAGTGCTTGATGACCTTCCCTTGCTTGTCTCT1650.11221665295129797No Hit
ATGTATGGGCAAAAAGTAATGTGTATTCAACTTATCAATAATAAATGTTAAT1650.11221665295129797No Hit
GCTAAGGGATACAATAAATTGTTGTTAACTGTGTATTACCCTGTTGGGCTAT1650.11221665295129797No Hit
GTTCCTGGGAGACGTCGGAAGAAACTCCCGTATTTGCAGCTGGAACTGCAGC1640.1115365520243204No Hit
ACAAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1640.1115365520243204No Hit
TTTTCAGGGTGAAATAGGGACCCCAGAAATTGACAGATGAAAGGAGACAATG1640.1115365520243204No Hit
ACCATAGGGAGATGCTGGGTGGAAAGGTCTCCAGCAGCAACCTCTGTCCTTC1640.1115365520243204No Hit
TGTCCAGGGGTCATTCTCCCTGTGGATAAAAGAGTTGTGGAGCCCAAATTCA1640.1115365520243204No Hit
AAGCCAGGGATCTGAAACAGTTGATAACAATTGAGTGTTGGCTGCCATGATG1630.11085645109734285No Hit
GCAATTGGGATGATGCTGATGCTGCTGATCCAGGGACCACACTTTGAGAATC1620.11017635017036527No Hit
TGGTACGGGATCTGGGAGTAAACTTAACATTCACTGTGTCTCTGCTCTGCAT1620.11017635017036527No Hit
ACTACAGGGTAACATGCCCGGCTTATTTTGTATTTTTAGTAGAGACAGGGTT1620.11017635017036527No Hit
GTCAAGGGGAGCCTCTTCCCTGTCATATTTTTCTCCATAGCCATCCTCCAGT1620.11017635017036527No Hit
AATCCAGGGATCCTGAGTGCCTGTCATGTCATAGTGTGGTGGACTTTTGCAG1610.10949624924338772No Hit
AAAACCGGGTTGCTCAAAAAATGTCTTTGAAATCTGTCTAAAGTATGTAGTT1610.10949624924338772No Hit
GCACCTGGGGTTGCCTCAGTGGTACTTTAAAAAATATCCTTTTATCACTAGA1610.10949624924338772No Hit
GGGATGGGGGAAATTAATATACTTATAAAATTAAGATTTATTAGCCAAACTT1610.10949624924338772No Hit
CTGATGGGGATTTCCGAATGTGTATGGCTGTATGGATTACTGGGCCTGGACA1610.10949624924338772No Hit
TTTCATGGGGTGGTGGTTGCACAACATTTTGAATGTTCTACATACAACTAGA1610.10949624924338772No Hit
CCGGCTGGGACGATCAGAAATTACACATTGAAGAATACTTCTTGATTTGGGA1600.10881614831641016No Hit
CGGTGTGGGATATCTATACCTATGGAAGATAGAAGGTACACAATTTTTTTTT1600.10881614831641016No Hit
CTTGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1600.10881614831641016No Hit
GCTGTAGGGTGGGACATAGAATGGGACTTCTTTTCCAAATTGGTAAATATGA1590.10813604738943258No Hit
CATTTCGGGATTGCCTGACAAGCTTGGATGCAAACAACCAGCAATGAGGCAG1580.10745594646245503No Hit
TCAATTGGGGAGGCTTGGTTGAACCGTGCAGATACCTTCTCGAAACAAAAGA1580.10745594646245503No Hit
ATCATCGGGGAACTTCAAGGGCTTGAAATTGCATTTAAAATTTAAAGAATAA1580.10745594646245503No Hit
TTTATTGGGCACCTGTGTTTTTCCAGATTTAAAGTAGAACCAAAATTGTAAA1570.10677584553547748No Hit
TATCTCGGGGACATTGGGGCGGGATGTGGGAGCGGCTGAACTGCGCAGCAGA1570.10677584553547748No Hit
ACTAATGGGATTGCTGAAGAGCACAATTTTTTTAAACTATTTACAGGCAGTT1560.1060957446084999No Hit
GTGTGGGGGCCGATCTGATAATGGGGAGGTAGGCATTGAGGCAGCCAGCGCA1560.1060957446084999No Hit
GTACTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1560.1060957446084999No Hit
TCTACAGGGGTAGAGAGGATCAATAAAGCCGACATTTTTTTTAAAGACTGAT1550.10541564368152234No Hit
AAGTCGGGGCTGCCCTTGAGAGGATCGACACATGTTTTTGTGGAGAATTCAG1550.10541564368152234No Hit
TGATATGGGATCTGTCATTCTAAGAAGACTGGACTCCATGCTAAAAGCAGTA1550.10541564368152234No Hit
CTTACTGGGGCCATAGGAAAACATGTGAGCAACAACCCCCATGAACATTTGT1550.10541564368152234No Hit
ATATATGGGGATACGATTATTTAGAAAACTTTTTCATTTTTTCCCATAAGGT1550.10541564368152234No Hit
TTGTAGGGAGAAAATAAAGTAAATTATTTTAAAAAATTACCTATATTCCCAA1540.10473554275454476No Hit
AGGTTCGGGATATCTGATCCCTGCAAAATACTTTGACTTGTCATTTTGTTGA1540.10473554275454476No Hit
GCTATGAGGTGCTGAAGTTCGATGACGTGGTCACCAACCTCGGCAATCACTA1540.10473554275454476No Hit
TAAGCAGGGACCTTTCCGAGTTGGCTGCAGATTTGTGGTGCGTTCTGAGCCG1530.10405544182756721No Hit
TCGCCGGGGGCCGAGAGAAGCAGTAGTCAATAAAGAGAGTGCCGTATTTCGC1530.10405544182756721No Hit
TTCTTGGGGATAAGGAGAAAGTCACCAACAGCTTGATCTATTATTTTTCCCT1530.10405544182756721No Hit
TCTAAAGGGACAGATTTATGATTATCATTTTGTCTTTTAATCATACGGGAAA1530.10405544182756721No Hit
TACGAAGGGGGCGGTGCTGGGCAGCAGACCGTCCAAACCGACACGCGTGGTA1530.10405544182756721No Hit
CTACCTCAGGGGATTCTGACCAAATTAAGCCTCTCAACTACTGTGGGAAAAA1530.10405544182756721No Hit
CATTATGGGCATCTGGTGCAAGACCAACCGGGAGACCATGGAATTGTCAAAA1530.10405544182756721No Hit
TGTCAAGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1520.10337534090058965No Hit
TACTTTGGGGATCGCGGAGTCGGTGCTTTAGTACGCCGCTGGCACCTTTACT1520.10337534090058965No Hit
ACGGTGGTAGGGCAAACTTCAAAGCAACAACAGTTAAAAGAGACAAAGAGGG1520.10337534090058965No Hit
AATCATGGGAGTGTATTGGAATTTTATCCTTTCTTATGGTGAATGGACAGTG1520.10337534090058965No Hit
AGCAATGGGGGAGTGCTTTAGCTCTGACTCAATACCCTCCTCTGCTAGAAAC1520.10337534090058965No Hit
GTAGATGGGACAGAAATAGAAAAATCTATTCTAAAAGTGACGCGAATCTCCA1520.10337534090058965No Hit
CGACTGGGAATCCCTAAATATGAATAGGTTTATATTGATTCATGGGTAACAG1510.10269523997361207No Hit
GTCATGGGGGGATACTTGAGTTTTTGCTTTTAAGGTGTTTGTTTAGGGATAC1510.10269523997361207No Hit
TCTAGTGGGTCCGATCTACTATGGGAATGATAGTTCTGATGTAACATTCGAT1510.10269523997361207No Hit
TTATCTGGGCAGTGAGATTTCTGGGAGAGAGATTGAACAATGAGCCATCAGC1510.10269523997361207No Hit
TGTTCTGGGGTATCTGGGACTGTTAAACTTATGTTGAAATATTGACATATTT1510.10269523997361207No Hit
CGAGCTGGGCCACTTCTGAACCCTGTTACCATTCCCCCTCACAAATCCCCAC1510.10269523997361207No Hit
AGAATAGGGGGGTGCTGTGCGCGCTGCTCCTGCTCTTGGTGCAGCTGCTGCG1510.10269523997361207No Hit
TTTTGAGGGATACTTATTACCATGACATCTGATGCATGTGCAGCAGTGGGGA1510.10269523997361207No Hit
CGTTCCGGGGGGCCAGTTGTGGGGTTGGTAAGCACAGTTATGAAAATTTCTA1500.10201513904663452No Hit
GATTTGGGGAATGCAAATGTCTAATTGTCCCCCAGTGGGCATAGTCAGTGTC1500.10201513904663452No Hit
TTCAAGGGGATTAGTTATTAGTTCCAAAGGACTGAGAACCAATTTTAATATT1500.10201513904663452No Hit
TTAGCTGGGAATCTAAACTAAGAGCCCCAAGGAGAGACATTTGAAGATTTGT1500.10201513904663452No Hit
GCATAAGGGGAAACAAGATGTAGACATTTTGACTACAGCATTTCAATTTCCA1500.10201513904663452No Hit
TTGCCACGGATAGTAATTTTTGGGCCTTACCCCAAAGTTCCTAAATTAGGAT1500.10201513904663452No Hit
CTCGCCGGGTAAGATTATATTTCCAGTACACTTCCTTAGGGCAGAAACACCA1500.10201513904663452No Hit
TAAGCTAGGGGACCATTCTAAATTTGTCTATTTCTCTAGTCCCTGATGAATG1490.10133503811965697No Hit
GCTGCTGGGACCGTGTACTTCAAAATACTTCTCACATGTCTGTTTTTATAAG1490.10133503811965697No Hit
CAAGTCGGGTGTCACTGGCTCAGGGGATTTTGATGTGCATGTTTTGCCTCAG1490.10133503811965697No Hit
GTTAATGGGTTGGTATCTTCATTTTACAGTTCATTAACCCAAGTACGTTTTC1490.10133503811965697No Hit
TGGCTGGGTTCTTCATGTTTGTCTTAGCTTGTTGTTGGTTTCCCACATCTAA1490.10133503811965697No Hit
GACTACGGGATGAAAATAATGTAATGGGATTCCAGAATGTTTATAATCAAAA1490.10133503811965697No Hit
TAGACAGGGGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCC1490.10133503811965697No Hit
CCCTGGTTCCTAGATGTGTTCTAAGCAATGCAAATGTCTAATTGTCCCCCAG1480.10065493719267939No Hit
GACTACGGGAGCTTTCCATAATTCAGTATCCCACATTATCTTCTGTTGTACC1480.10065493719267939No Hit
AATACGGGTCTGCGCATGGGGGAAACACAAGTTAACTAGTGTTTAGTTTAAC1480.10065493719267939No Hit
CACTAAGGGATCTTCTTGGTCTAGAAAGTAAAATTATAATGCTTGGCATAAT1480.10065493719267939No Hit
CATGGGCAAATAAGTGAAAGATCTCCATAAGGAGAGCTACAAAAACTGCTGA1480.10065493719267939No Hit
ATATAGGGGTGCTATTGTGGGTGTTTATGCTCCAGTGGTCCACCTCTGGCAA1480.10065493719267939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)