FastQCFastQC Report
Sat 31 Dec 2022
SRR5848400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146362
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGC5710.39012858528853117No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGCCGTCT5210.3559667126713218TruSeq Adapter, Index 5 (95% over 21bp)
TGATTTGGGCTCTTTGAAGTGGAAAGAACTGATGCATTAAACATTCCTTAAA2030.1386972028258701No Hit
AAGCATGGGGATGGAGGTTCCACAACAAACAAAAAATAGAATTATCGTATGA2000.13664749046883753No Hit
AGTTCCGGGTCTCATGGATTTGTTAGGAGTTCTCTTGTATTTTCTGTATTTT1980.13528101556414918No Hit
AGCGTAGGGATAGCATGCAAAAAAGAAACTATATTTTCTGTCCTGTTCTTTG1950.1332313032071166No Hit
TGTCCAGGGGTCATTCTCCCTGTGGATAAAAGAGTTGTGGAGCCCAAATTCA1940.1325480657547724No Hit
TGATGAGGCACACTACTGCATGACTACAGATTTAGACATTCTTAAGGAGCTC1920.13118159085008405No Hit
TGGTACGGGATCTGGGAGTAAACTTAACATTCACTGTGTCTCTGCTCTGCAT1890.12913187849305147No Hit
ATGATTGGGGAATCCAATAAAGAGAGGCAGCTTGGAAGTAAGTAAATTTTTG1870.1277654035883631No Hit
GCCAGGGCGATCTTTGCAAGGGTCAGCCGAGCTGGACGAGCAGGACATCTTG1840.12571569123133053No Hit
GGAGCTCCTCATGTACTCCCATGACAACATCATCTGTGGCATCACCTCTGTT1800.12298274142195377No Hit
CTCGTTGGGAGATGATGATAATGGAGGAATACTCGTGGACAAGATGAGTTAA1790.12229950396960959No Hit
GACTTAGGGCAAACAGAAATAGTTTTATTACTTGCTTTGTAATTTCCTTTTA1780.12161626651726541No Hit
CTAAATGGGGTGGTGAGAAAGTTCTTGAGGATGGTAAGGAGCTCCACATTTT1770.12093302906492122No Hit
TCGATAGGGGATTTATGCATTTATGCATGAGAACTAAATAGATTTTTAGATT1760.12024979161257704No Hit
TTTCATGGGGTGGTGGTTGCACAACATTTTGAATGTTCTACATACAACTAGA1750.11956655416023286No Hit
CTTGTTGGGGCACCCACTGCTGCACACTAAACTGTCTGCCACCGGCTCCCTC1730.11820007925554447No Hit
GAACTAGGGATTTTCAATATTTTTCCTCTCATTTGGTCCTCAGAACTATTCC1730.11820007925554447No Hit
TGCTCCGGAGTTGGGAAGGTGGAGAGAAGTGGTGGGGGGCAGGAAAAGTTTT1730.11820007925554447No Hit
TTAACTGGGGAGGCAGGAGAATTGCTTGAATCCGAGACATGGAGGTTGCAGT1730.11820007925554447No Hit
TGTCAGGGGTGCTCCTGAAATCAACCAAACAACCAAACAGACTCTCTAGTCT1730.11820007925554447No Hit
TGTCAAGGGATATTGACAAATTTATTGAAGACACAGAAATTTTACTAATGCT1720.11751684180320028No Hit
CACATAGGGATTCAAGTGCGCAGGTAATTACTTGGTTGAAACAATAGTGTTT1700.11615036689851192No Hit
AGAATAGGGGGGTGCTGTGCGCGCTGCTCCTGCTCTTGGTGCAGCTGCTGCG1700.11615036689851192No Hit
GTAGATGGGACAGAAATAGAAAAATCTATTCTAAAAGTGACGCGAATCTCCA1700.11615036689851192No Hit
CGACTGGGAATCCCTAAATATGAATAGGTTTATATTGATTCATGGGTAACAG1690.11546712944616772No Hit
CCGGCTGGGACGATCAGAAATTACACATTGAAGAATACTTCTTGATTTGGGA1680.11478389199382354No Hit
GACTACGGGATGAAAATAATGTAATGGGATTCCAGAATGTTTATAATCAAAA1680.11478389199382354No Hit
ACCACTGGGCTCTAAAAGGTTAATTTCTGTCAAATGCAGTAGATGATGAAAG1670.11410065454147934No Hit
CACGTGGGGATGCAGCAAAGACCTAGTATTAAGGAAGTTTCCCACAAAACAA1670.11410065454147934No Hit
AGCAATGGGGGAGTGCTTTAGCTCTGACTCAATACCCTCCTCTGCTAGAAAC1670.11410065454147934No Hit
GGGATGGGGGAAATTAATATACTTATAAAATTAAGATTTATTAGCCAAACTT1660.11341741708913516No Hit
ATCATCGGGGAACTTCAAGGGCTTGAAATTGCATTTAAAATTTAAAGAATAA1660.11341741708913516No Hit
AAAACCGGGTTGCTCAAAAAATGTCTTTGAAATCTGTCTAAAGTATGTAGTT1650.11273417963679098No Hit
CCGGCTGGGAATTCCACCTGAGAACAAGTCCCGCAAACCGGGGACGGAAGGA1650.11273417963679098No Hit
GTGCCAGGGACTGTGATGCCGGCAGGCAGAGCAGCTACTGACTTTGAACATG1640.11205094218444678No Hit
TGTGTTGGGAATGCCAGCCTCTGTGCCCGACCTCGGAAAACTGGCCATGCCC1640.11205094218444678No Hit
TGTTAAGGGATGTAAATGTGAATGGAAACTTGGAAATACTCGTTTTTATAAA1640.11205094218444678No Hit
CTGATGGGGATTTCCGAATGTGTATGGCTGTATGGATTACTGGGCCTGGACA1640.11205094218444678No Hit
AACCAAGGGGTTAACAAATGTGGCAATTATTTTGGATCTATCACCTGTCATC1640.11205094218444678No Hit
TAAACAGGGTGTCTTGGCGTATTGCCAATTCACCACTTTCTACTTTGTGCTG1640.11205094218444678No Hit
TATCTCGGGGACATTGGGGCGGGATGTGGGAGCGGCTGAACTGCGCAGCAGA1640.11205094218444678No Hit
AAGCCAGGGATCTGAAACAGTTGATAACAATTGAGTGTTGGCTGCCATGATG1630.1113677047321026No Hit
TACGAAGGGGGCGGTGCTGGGCAGCAGACCGTCCAAACCGACACGCGTGGTA1620.11068446727975839No Hit
CACCATGGGGGGGTAAGGATTCGGCGGGCAAGCGGGTGTAATCAGCAGCCAT1620.11068446727975839No Hit
AAGTTAGGGGACCAGCACCATGGCGGTTGGCAAGAACAAGCGCCTTACGAAA1620.11068446727975839No Hit
GTCATGGGGGGATACTTGAGTTTTTGCTTTTAAGGTGTTTGTTTAGGGATAC1610.11000122982741421No Hit
TCAAAAGGGGATCTGTCTAATGTTGACAGTGGGGTGTTAAAGTCTTCCATTA1610.11000122982741421No Hit
CTTGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1610.11000122982741421No Hit
CTGTACGGGTCAATTATGAAGAAATAGAAAATCTTAACAAACCATTAATGAC1610.11000122982741421No Hit
CAAGGGGCAGAAGTTTGAGAAGGTAAAAATTAAGTTGTAGTATCTGAGTTGT1610.11000122982741421No Hit
ATGATCGGGCATTGCCTGCACAGTGCTTGATGACCTTCCCTTGCTTGTCTCT1600.10931799237507003No Hit
ACTACTGGGGCTCTGTAAGACATGTGAGTGGAAAACTTTAAAATGTTATAAG1600.10931799237507003No Hit
TTATAAGGGGATTCCCTCAACCAGAAGGTAAGGTAGAGATTCCTGGCCCTGG1600.10931799237507003No Hit
TTTGGTGGGAGTCTTCAGTGATCATGATTCACTAACTTTCTGTGCTAGATAT1590.10863475492272584No Hit
TAACTGGGAGCCTCCACCTCCTGGGTTCAAGTGATTCTTGTGCCTCGGCCTC1590.10863475492272584No Hit
TCTACAGGGGTAGAGAGGATCAATAAAGCCGACATTTTTTTTAAAGACTGAT1590.10863475492272584No Hit
GCACCTGGGGTTGCCTCAGTGGTACTTTAAAAAATATCCTTTTATCACTAGA1590.10863475492272584No Hit
CAGCTGGGGAGAGGAGGCTGGCTCAGAACACCTAGAGGAGGGGGCCGGGGAT1580.10795151747038166No Hit
TGGCATGGGAAGAAACAACTGTCAGGGCAACCAGTATATTTGAGAGGACATG1580.10795151747038166No Hit
CGGTGTGGGATATCTATACCTATGGAAGATAGAAGGTACACAATTTTTTTTT1580.10795151747038166No Hit
AAAGTTGGGTCCAGCCCAGTGTCAAGTCCCTGCTGCAGGTGCTGGGCTCTGT1580.10795151747038166No Hit
GCATAAGGGGAAACAAGATGTAGACATTTTGACTACAGCATTTCAATTTCCA1580.10795151747038166No Hit
TTTAATTGGGGAGGTTGCAAATGTTTCAAGCATGTGCAGTTTTGTGTGTGTG1580.10795151747038166No Hit
ATTGGTGGGAAGGTCCTGCCCTGAAGGTGACAGCTCTACCTGTACTGACCAA1580.10795151747038166No Hit
TTACCTGGGGTGGTCGCTGTAGTCTTCTGCCAGTTGTAAATAATTCGGGTGC1580.10795151747038166No Hit
CGTTCCGGGGGGCCAGTTGTGGGGTTGGTAAGCACAGTTATGAAAATTTCTA1570.10726828001803745No Hit
GCAATTGGGATGATGCTGATGCTGCTGATCCAGGGACCACACTTTGAGAATC1570.10726828001803745No Hit
AGGTATGGGAAGCTAGCGGAAGACAAGAAATAACTAAGATCAGAGCAGAACT1570.10726828001803745No Hit
TGTAACGGGCTCTCATGTTGGACACGGGCCTGCCCTGTTTTCGCGGCCAGAC1570.10726828001803745No Hit
GTCAAGGGGAGCCTCTTCCCTGTCATATTTTTCTCCATAGCCATCCTCCAGT1570.10726828001803745No Hit
TACTTTGGGGATCGCGGAGTCGGTGCTTTAGTACGCCGCTGGCACCTTTACT1560.10658504256569327No Hit
AAAAGAGGGGATTCCCGGAGGGCAGTTGGGGAGCGCAGATCCCGAAGCAGCG1560.10658504256569327No Hit
TTTAAAGGGATGTACAAAGCATTATTGAAACAGCGATGATATAAAAAGAAAT1560.10658504256569327No Hit
TATAGTGGGCAACCAGAATAACTCCTCTTAGAAGAATCATCATCCAGCACTA1550.1059018051133491No Hit
TGAACTGGGCATGTTTATCCTGTTGCTCAGTGAATCCTCAAATCGTTAATTT1550.1059018051133491No Hit
CCATTGGGGGAGCAGCTCCTGCCGTTCTTCAATGTGCTCAGTCAAGTCTTCA1550.1059018051133491No Hit
TTTTGAGGGAAGTTTTCATTGGTAATGGATTTATTCCAGACAGGACAGGTTA1550.1059018051133491No Hit
AGGTTCGGGATATCTGATCCCTGCAAAATACTTTGACTTGTCATTTTGTTGA1550.1059018051133491No Hit
TCGCCTGGGACTCAGAGAATGTTTAAAGAAATGAGGAATGAAGAAAGGGAGT1550.1059018051133491No Hit
CTACCTCAGGGGATTCTGACCAAATTAAGCCTCTCAACTACTGTGGGAAAAA1540.1052185676610049No Hit
TTTTCAGGGTGAAATAGGGACCCCAGAAATTGACAGATGAAAGGAGACAATG1540.1052185676610049No Hit
GAGTCTGGGAGGATGGTCTCGATCTCCTAACGTCGTGATCCTCCCGCCTCGG1540.1052185676610049No Hit
GGTCAGGGGAGGGTGACAGACTTCACCGGAGATCAGAGATCAGTTGATGCAG1540.1052185676610049No Hit
TATATTGGGGCAGTGGCAGAAGTGGATTTTTGGGGGTACGTTTTCAGATGTG1530.10453533020866072No Hit
GTTAATGGGTTGGTATCTTCATTTTACAGTTCATTAACCCAAGTACGTTTTC1530.10453533020866072No Hit
GCGTAGGGGGACCACCCTGGGCACAGCAGGCATCGGAGGTGAGGCGGGGTTG1530.10453533020866072No Hit
TAACAAGGGGAGGAAAATTTTTTCATTTTATTGACAAGCATATGAGACTCAG1520.10385209275631654No Hit
TTCTTGGGGATAAGGAGAAAGTCACCAACAGCTTGATCTATTATTTTTCCCT1520.10385209275631654No Hit
GATTTGGGGAATGCAAATGTCTAATTGTCCCCCAGTGGGCATAGTCAGTGTC1520.10385209275631654No Hit
ACGGTGGTAGGGCAAACTTCAAAGCAACAACAGTTAAAAGAGACAAAGAGGG1520.10385209275631654No Hit
TGCTTTGGGATATTAGACGTAACCCCAGGGACCTATGGAAAAATATCAAAAG1510.10316885530397234No Hit
AGCATAGGAATAGTGGGAGATTTCAATACTCCACTGACAGCACTAGACCAGC1510.10316885530397234No Hit
AGAAAAGGGATCGCGGCTAGAGAGATGTCGCTGCTGCGGTCGCTGCGCGTGT1510.10316885530397234No Hit
GTTCAAGGGGTGGAACAAAATCCTGTTTTTAGAGTGTTTAACTTTTACTTAT1500.10248561785162816No Hit
GTACTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1500.10248561785162816No Hit
TGATATGGGATCTGTCATTCTAAGAAGACTGGACTCCATGCTAAAAGCAGTA1500.10248561785162816No Hit
TCTAACGGGGCTCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAA1490.10180238039928396No Hit
GTTTCAGGGTTTGTGTATAAATCAGTGTCAGTGTCTCTCTTTTCTTAGATTT1490.10180238039928396No Hit
GCAGACGGGGGTTATCTCATTGGCTATAAAAGAAGAATTTACAACAGTTGAG1490.10180238039928396No Hit
ACACATGTTTATTATTGTCTAGCGTCTCCTCCATTATTGAAAGTGGGTATTA1490.10180238039928396No Hit
GGGAGTGTTCTGATTGGTTCTAAGGTAAACTTTTGTTTTTGAGAAACAACAG1490.10180238039928396No Hit
GTGTGGGGGCCGATCTGATAATGGGGAGGTAGGCATTGAGGCAGCCAGCGCA1480.10111914294693979No Hit
GCTTTGGGGATATTTTAAATAGACTAACTTTTAAGTGAATGCTTTGTACTTT1480.10111914294693979No Hit
GCGCAAGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT1480.10111914294693979No Hit
TCTAGTGGGTCCGATCTACTATGGGAATGATAGTTCTGATGTAACATTCGAT1470.1004359054945956No Hit
ATTTTTGGGTAAGACATTGTGTAAAAAGCAATCTGTAAAAACATCTCCAGGC1470.1004359054945956No Hit
CAAATGGGGGATCTTTCTGCGGCGCGCGGACACCCGCCGGTGGAAGAAGAAA1470.1004359054945956No Hit
TGCCCCGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGA1470.1004359054945956No Hit
ATATATGGGGATACGATTATTTAGAAAACTTTTTCATTTTTTCCCATAAGGT1470.1004359054945956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)