FastQCFastQC Report
Sat 31 Dec 2022
SRR5848372.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848372.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268253
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGC10560.39365822563028185No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGCCGTCT9940.3705457161709282TruSeq Adapter, Index 5 (95% over 21bp)
GCGGCTGGGAGAAACACTGTAGATAAATCAAAATTGAATTTAAATAATGTTA3370.12562767238390624No Hit
GTAGGCGGGCCAATGCTATTTAACAAATTGAAGAATACCATCAGCAAGTTTT2940.10959802872661256No Hit
CCTTATGGGAACAGCTTATAGAACTTGAACTACTAAATATGAAAATAAGTCA2860.10661576944153467No Hit
CGACGGGATACAGAATCCGACGTGAACAAGGAGGGCTTGGAATGCCTTCGGC2840.10587020462026521No Hit
CTTGCTGGGATTCTTCAAAAGTTTCCCAGGTGATCCTGGAAAATAAATTTTG2830.10549742220963046No Hit
TTTGCCGGGGTTTATAAAAGGTTTTAAGTCTGTTGCCTGATTTTTAAATTTA2820.10512463979899572No Hit
GCTCACGGATGGTGTGAATGTTGTGACAGGAAGTCTAAGAAAAGAAAGGGAG2810.10475185738836099No Hit
TTAAAGGGGATAAATAGTTGGGAAAGCTATCCTGGTATATTTTCATAAGTAA2780.10363351015645678No Hit
GTTTGCAGGAGGAACTGTGAAGTTAACTGTCTCATGGGAGATTTCTGTTAAA2770.10326072774582205No Hit
ACAACAGGGGTGGCCAGCCTCGACACAGATTCCACCAAAAGCTCAGGACAAA2760.1028879453351873No Hit
AAAATAGGGGCCCGCGGCCTCGTCGCTCCTGCTGAGGCGGTAGCCGCTTTTC2750.10251516292455257No Hit
AGCCCTGGGGTTGTGACGCTGTTGATTCTGAGGCCCGAAATCCCCCTTAATG2740.10214238051391783No Hit
AGATACGGGCAAGCTTCAACCCTTGCCTGATTGTTGCCAACATTTACTTGCA2730.10176959810328311No Hit
CCGACTGGGATAACCAACAACAGGGGGAAAAAAAGATACCCAGATTCCAAGG2700.10065125087137887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)