FastQCFastQC Report
Sat 31 Dec 2022
SRR5848371.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848371.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267216
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGC10330.38657864798515057No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGCCGTCT10050.37610023351895094TruSeq Adapter, Index 5 (95% over 21bp)
GCGGCTGGGAGAAACACTGTAGATAAATCAAAATTGAATTTAAATAATGTTA3410.12761211903478833No Hit
TGCCATGGGGGGGAGAAAGATGGCGGCAGCCGTGCGACAGGATTTGGCCCAG2870.1074037482785462No Hit
GGCTGTGGGAATGAAATGCCGGGTGTGGTGGTGCACTCCAGACTGGGCGATA2850.10665529010238908No Hit
ATTCTCGGGGTAAAATTTAGGTAAGAAATAACTGGACAGTGAGATTTATACC2830.10590683192623196No Hit
AATCATGGGTAAGCACATTATTTTATTAATAGGGTTCAATGTCTTTTCTCTA2810.10515837375007485No Hit
CAGCTCTGGGAAGGGCAGCGGCTGAGACGACTTATTAGAGGAGTCATGAAGG2810.10515837375007485No Hit
GTAGGCGGGCCAATGCTATTTAACAAATTGAAGAATACCATCAGCAAGTTTT2800.10478414466199629No Hit
CACGCTGGGGGTGCTTTCACTCTTAGTGAGAAAAAGACCTAACTAATTGTTA2780.10403568648583918No Hit
CGACGGGATACAGAATCCGACGTGAACAAGGAGGGCTTGGAATGCCTTCGGC2780.10403568648583918No Hit
GTGGCAGGAGCCGCGGAGCCTGGCCTAGAAAGGAAAATTTAAGTAAGATACC2760.10328722830968205No Hit
GCTCACGGATGGTGTGAATGTTGTGACAGGAAGTCTAAGAAAAGAAAGGGAG2740.10253877013352494No Hit
GGCTAAGGGGCCGACCACGGATTTGCATTGCCGAGGACGGGACCCCAGGGCA2740.10253877013352494No Hit
CATTACGGGGTGTTTTTATTACTTTCATCTGAAACATTCCATGTTTTAATCT2720.10179031195736782No Hit
TACCATTGTTCCCACTTCATAGGTATGGAAACTGAAGGTTAGAGAAGTGATA2720.10179031195736782No Hit
AAAATAGGGGCCCGCGGCCTCGTCGCTCCTGCTGAGGCGGTAGCCGCTTTTC2720.10179031195736782No Hit
AATATGGGCGGAGGAAAAGAAATATTTTTGACTAAAGTTTTTAAACTATAGT2720.10179031195736782No Hit
CACGCTGGGAGAACAAAGCCCGTTTAACAACTGCAAAGCACACAGTCTGCAG2710.10141608286928926No Hit
AATTTGGGGGTGGTGAGAATGGTTAAAATCTACTCTTTTCACAATTTTGAAA2690.10066762469313215No Hit
GTTTGTGGGACGTAGGTTAAGTACATGTGTGCATTTTCTTGGCCACTTACTT2680.1002933956050536No Hit
CCAATTGGGATGACCTGATGGACAACAAGGACAAGGGCAGCTCCTAGGAGCT2680.1002933956050536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)