FastQCFastQC Report
Sat 31 Dec 2022
SRR5848370.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848370.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265990
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGC10000.3759539832324524No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGCCGTCT9630.36204368585285157TruSeq Adapter, Index 5 (95% over 21bp)
GCGGCTGGGAGAAACACTGTAGATAAATCAAAATTGAATTTAAATAATGTTA3530.13271175608105568No Hit
GCTCACGGATGGTGTGAATGTTGTGACAGGAAGTCTAAGAAAAGAAAGGGAG3130.11767359675175758No Hit
ATTCTCGGGGTAAAATTTAGGTAAGAAATAACTGGACAGTGAGATTTATACC3070.11541787285236287No Hit
AGCCCTGGGGTTGTGACGCTGTTGATTCTGAGGCCCGAAATCCCCCTTAATG3010.11316214895296815No Hit
CATTACGGGGTGTTTTTATTACTTTCATCTGAAACATTCCATGTTTTAATCT2980.11203428700327081No Hit
GCAGCGGAATTTTAGATAATTTTTAAATTCAAAGTTATTTATAAAATGCTAG2930.11015451708710852No Hit
AAGCTTGGGTATGAAAGAAAAGGACAAACCTATAAATTAACTCAACCTATAT2900.10902665513741118No Hit
GGAGTAGGGAAGCAAATCAAAGTTGAAGCAGGTTCTATTTCATTCAACAAGT2880.10827474717094628No Hit
CGACGGGATACAGAATCCGACGTGAACAAGGAGGGCTTGGAATGCCTTCGGC2880.10827474717094628No Hit
TGCACTGGGAAATAGGAAAAATGTATAATGCAATACAAATCTGGGCAAAAGA2860.10752283920448136No Hit
TTCGGATGGGCAGGGATGCTCTCGGTGAGTTATCACAGAAATGATGTTGCAG2810.10564306928831911No Hit
GTAGGCGGGCCAATGCTATTTAACAAATTGAAGAATACCATCAGCAAGTTTT2790.10489116132185419No Hit
GGTTGTGGGAGGATCAGTTAGCTGCTGACAAGAAAAGTCATCTTGTGTCTTT2770.1041392533553893No Hit
CATCGGGGGAGATCATTTTAGTTTTACACTTTTGTGTGGCTGAAAGAAAAAA2760.10376329937215685No Hit
TTAAAGGGGATAAATAGTTGGGAAAGCTATCCTGGTATATTTTCATAAGTAA2720.10225948343922706No Hit
ACAGCCGGACTGGGAAGTGAGGAGCCCCTCTGCCCGGCCACCACCCCGTCTG2710.10188352945599459No Hit
CCAGCCTGGGTGACAGAGCAAGACTCCGTCTCATAAAACAAAAACAAGAAAA2690.10113162148952969No Hit
GTCTCAGGGAGAAGGTGGTTTATAATGTTTGTCTCAAAAGAAACATAAACAT2680.10075566750629722No Hit
AAAACCGGGGTCTGCAACCTGACACTGCTGACAAGCAGACAGACACACAAAC2670.10037971352306477No Hit
CTTGCTGGGATTCTTCAAAAGTTTCCCAGGTGATCCTGGAAAATAAATTTTG2670.10037971352306477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)