FastQCFastQC Report
Sat 31 Dec 2022
SRR5848369.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848369.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190102
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGCCGTCT13720.7217178146468738TruSeq Adapter, Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGC10930.5749544981115401No Hit
GCATCCGGGGATGTGTGGATTAGTGAGGAAAAAGAAACAAAGGAAACTCAGT2660.13992488243153675No Hit
GTCAATGGGACACAAACATGTCTGCTAATGAACTGATGATATGTTAAATAAT2510.13203438154254032No Hit
TAGAGCGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT2450.12887818118694175No Hit
AGTCAGGGGGCAGATACCACATTAATCAAGTGAAAAACATTAATAACATCGT2450.12887818118694175No Hit
CATATGGGCAGTAGGTTAAAAATATTTTAATCAACTGACTGCTAAACACATC2310.12151371369054507No Hit
CATTGCGGGATGGCTATTGAAATTATGAGTGTTGATCTGTTCTATAAATTGG2290.12046164690534555No Hit
AAATCAGGGATAATTCTGGACCCCTATTTATCTCAAAACTCTTAATATATGC2280.1199356135127458No Hit
TGAGGGGGCATTGTTGATATTTTAGTAGCTTTCATTATCTGCTACAGCCAGA2280.1199356135127458No Hit
GTAAGGGGGCTCACTGCAAGCTCCACCTCTTGGGTTCACACCATTCTCCTGC2270.11940958012014602No Hit
TCAGTCGGGATGTCCCCATCGTGCTTTGTGCACACTCCTTTTTTGTATGTTT2260.11888354672754628No Hit
AGTAGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGG2240.11783147994234675No Hit
AATAGCGGGCATATAAATTACACAAATCAAATCTGTCGACATCCCAAGAATA2230.11730544654974698No Hit
GCCATAGGGAGCCCAGGCAATTCAGAAATTTATCAGATAAATTTAACAAAGA2230.11730544654974698No Hit
CAACTTGGGAACTCAAAATGGATTAAATATTTAAATGTAAGACCTCAAACTA2210.11625337976454746No Hit
TGATATGGGAGAAAGGGATGGATGAGAAGACAGACCTGAGACCAATCTGGGT2180.11467527958674817No Hit
ATTGTTGGGTTAGAAACAAAAACCATGTTGGAAGACCAACTAGAGGGAACTC2160.11362321280154865No Hit
TCATGTGGGATTGGGGCCAAGGCGGACTCAGCTCCGGGGAGAAGGGCCTCTC2140.11257114601634913No Hit
AGCTTGGGGATAGTAGTCAAAAGGATAATTTAATAAGCATTTTACGTTACTA2140.11257114601634913No Hit
CGAAACGGGACCTTTTAAAGAACAGAAAAATGAGCAACTGAAATTTTGAGAA2140.11257114601634913No Hit
TGCAAGGGGGAAATAAGCATAATTGCTAATACTAAGATTTAGCAATATTGTA2140.11257114601634913No Hit
TACCACGGGGGTCTTCTGTTTAGTGTTGTATTTCAATTGTGGAGCCTCATTT2130.11204511262374935No Hit
TTTCTGGGATTCAATAAATGGTGTTGGGATAACTGGCTAGCCCTATGCAGAA2130.11204511262374935No Hit
GACTCCGGGCAGCACACATCTCTGTGATGTAGGTACTACCTTATGCTCACTT2130.11204511262374935No Hit
GCTATGGGGACATATCTTGGTTTTTCTCCAAGCTTTCTGGCAACTTTTCTCA2120.11151907923114959No Hit
GCTATTGGGGTTGTACAGGGTGCCAGATAGATTGGAAAAAAGGAAAGAATGT2120.11151907923114959No Hit
ATAGTTGGGTCTGGTTCCGGGAGATGTTGAGCATCTTTTCATGTGCTTATTG2120.11151907923114959No Hit
AGTTGGGGGGTTCTTTGAATGGTTGCTGATGTTATTTAGCAATAATTCAAAT2110.11099304583854983No Hit
ACTTCTGGGATACATTTATGCACATTCACGCTGTTTGTTTCATATATACAGG2110.11099304583854983No Hit
GCTAGTGGGAGAACCAGGTTTTTGTTTTGATGATTTATCCAGTTGTTTTCTG2100.11046701244595007No Hit
CAATACGGGATAATTACAAATATCCGAAAGTCATTTCTTGGAACACAAGTGG2100.11046701244595007No Hit
TACTATGGGAATGTCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGG2080.10941494566075055No Hit
TCGTGAGGGCACATAACTTTATTGAGCCAAGCGAAAAGAAAAGAACAAGATC2070.10888891226815077No Hit
TGCTGAGGGCGATCTGGTTATGGGCACTGAGATTCAGGTTTAGGAAGCTGAC2070.10888891226815077No Hit
CTTTCAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2070.10888891226815077No Hit
TCTGGAGGGACAGATAGAATTTTTATTAAGTGGTTTGTTGAAAGTTTGTCAG2070.10888891226815077No Hit
ACAGCTGGGAGTTTGCCTCAGCTGTCAACATGCTGTCCTCAATAAGCTTAAT2040.1073108120903515No Hit
GTCAACGGGATTTTTAATAGATGAAGGAGGATTTGGGACTTCAAAAATGTAA2030.10678477869775173No Hit
GCCTGCGGGCATCCTTCTCTGCTTGACATTCAGTTTTACAGAAAATCAGGAA2030.10678477869775173No Hit
TCATTAGGGAGAACATTGTGATCTATGGTATTGAAAAGCAGGAATAAAGAGA2030.10678477869775173No Hit
TAACCAGGACCAGGAACCATTCAAGTAAATGGCCATGGAGGACAGCCATCAA2030.10678477869775173No Hit
AAAGCTGGGCTGCCAAAATTATGGATCCGGATGAGCAATTGGAAACCCTTCA2030.10678477869775173No Hit
CGACCCGGGACATCAATTTTCTTCCAAGATTTAATTTGGCACACAGGGTTTT2030.10678477869775173No Hit
GCGATGGGGTCGTAGGTATTAGTGCTAGTCTCAAATAGGTGCTTCCCCTGAG2020.10625874530515197No Hit
CACAGTGGGACAGACCAGCAGTCACAGAAATCATTCTTCCTGCTGTACTGGG2010.1057327119125522No Hit
AGGGTTGGGAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATG1990.10468064512735267No Hit
ACGTCAGGGGAGTTTCTACCAAGATCTCCACAATACTATGGTCAAATTAACA1990.10468064512735267No Hit
ATAACTGGGAAATTCATGCTGTTTCATCATTGCTGGTGTATTCATATGTGAA1990.10468064512735267No Hit
ACACTGGGGATATGGCATACCCTAGGGTATTCACTTTGGAGAAAACTCAAAA1980.10415461173475292No Hit
GCTGACGGGCTAATTAACTTGATAAAGCTTTACTCTCATTATAAGCCCAGTT1970.10362857834215315No Hit
CGCTGGGGGTATGCAGTGTGATCTTTATTTATAGTAAATTATGTTTCATGTA1970.10362857834215315No Hit
TATCACGGGACGCAGATGTAATGCACATTATTGGCGTATCTTTAAGTTGGAT1970.10362857834215315No Hit
TTCTACGGGTATTACTGTGGTATTAGAATCTTTGGCATGGGAGATTGATGGC1970.10362857834215315No Hit
TTGCCAGGGCAGGGTTCTCTCTCCTGAGCCTAGATTTGCCAGGGTCTTGGTG1960.1031025449495534No Hit
CACCGTGGGGCAATGATTTTATCCCCCAGAATCCTTCACCTTTCAAGGAGTT1950.10257651155695365No Hit
TATCGCGGGACTTGGATAACTGGCTAATCATATTAAAGGACTATGTGGTTCC1950.10257651155695365No Hit
CGTTTGGGGCAATATGCAACTGATCCCGCTTTTATTAATTTTTTGAAGAAGT1950.10257651155695365No Hit
GCCTAAGGGGTTTTTAGTAAAGTGCCTGTGTTCATTGTGGACAAAGTTATTA1940.10205047816435388No Hit
CATCTTGGGCTGTCAAGTTTTATTACAAAATTACTTGTTAAAACAATTTTTA1940.10205047816435388No Hit
CCATCCGGGTCTCTTGACCTCGTGATCCGCCCGCCTTGGCCTCCCAAAGTGC1940.10205047816435388No Hit
CATTATGGGATTTCTGCTTTGGGATCTGGAAGTGTTCCTCTGCTCGCCTCAT1940.10205047816435388No Hit
CGACCAGGGGCTTCTCACTCACTCAGGACACTTCTTGAGGCAATGTAGACAC1940.10205047816435388No Hit
GACTAGCGGGCTGGCAAAATGTGTTTACGTATCTGACAAGTTGTACATTTGT1930.10152444477175412No Hit
TATGCAGGTGACTACCATCTCTTCTGCGCGCTGCTCACTGGCTTCCTCTTCG1930.10152444477175412No Hit
CTTGATGGGAACCTGACAGTGAACTATAATGCAGAAAAAGTGATCTCCTATG1920.10099841137915434No Hit
GTGATGGGGAATTAAACATATCAATTTCAGAATGATTAATGGAGCCACAGAT1920.10099841137915434No Hit
TCCATAGGGATGCATGGGATGGCAAAAGTGTGGTTCCAGAAAAGCCAAAGAA1920.10099841137915434No Hit
AACTAAGGGTACATCATTAACCTCACTCAGCCCTACTGCCCATCTATAAAGT1920.10099841137915434No Hit
TTGTATGGGACCACTTTTTGTATTTGTCATGATATTTTTGACCCATTGGCAA1910.10047237798655459No Hit
AATCATGGGATACAAAGAAGTGTTTCATATAGCTAAGTCTCAATTGTTAATC1910.10047237798655459No Hit
GCTAAGGGCGATCTGAATCGGGGGATCCTTGTTCAAGTCTATGTTATGGATT1910.10047237798655459No Hit
CTCTCTGGGAACACTACCAAGGAAACATTAAATTGTCTGCTTGAGGTTTTTT1910.10047237798655459No Hit
TAACCTGGGGAATTTATTTATTGGTTTACAGTTTTATCTTGCTGGGCAGGAC1910.10047237798655459No Hit
CGTAGTGGGATCCACAGAGGAAATTATACACTTCCATCTTTGTTTCATAAAT1910.10047237798655459No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)