FastQCFastQC Report
Sat 31 Dec 2022
SRR5848368.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848368.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189919
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGCCGTCT13150.692400444399981TruSeq Adapter, Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGC9630.5070582722107846No Hit
GCCATAGGGAGCCCAGGCAATTCAGAAATTTATCAGATAAATTTAACAAAGA2650.13953316940379845No Hit
TAGAGCGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT2510.1321616057371827No Hit
TCTGGAGGGACAGATAGAATTTTTATTAAGTGGTTTGTTGAAAGTTTGTCAG2500.1316350654752816No Hit
TGCAAGGGGGAAATAAGCATAATTGCTAATACTAAGATTTAGCAATATTGTA2420.12742274338007256No Hit
CATATGGGCAGTAGGTTAAAAATATTTTAATCAACTGACTGCTAAACACATC2400.1263696628562703No Hit
TCAGTCGGGATGTCCCCATCGTGCTTTGTGCACACTCCTTTTTTGTATGTTT2370.12479004207056692No Hit
CGAAACGGGACCTTTTAAAGAACAGAAAAATGAGCAACTGAAATTTTGAGAA2350.12373696154676468No Hit
TGATATGGGAGAAAGGGATGGATGAGAAGACAGACCTGAGACCAATCTGGGT2320.12215734076106129No Hit
CAATACGGGATAATTACAAATATCCGAAAGTCATTTCTTGGAACACAAGTGG2300.12110426023725905No Hit
GTCAATGGGACACAAACATGTCTGCTAATGAACTGATGATATGTTAAATAAT2290.12057771997535792No Hit
GGCGCTGGGAGTCCTTCTGGTCAGTGAACGAAAATTCTAGACCTACAGTTAC2250.1184715589277534No Hit
AGTCAGGGGGCAGATACCACATTAATCAAGTGAAAAACATTAATAACATCGT2250.1184715589277534No Hit
ATAGTTGGGTCTGGTTCCGGGAGATGTTGAGCATCTTTTCATGTGCTTATTG2240.11794501866585229No Hit
CATTGCGGGATGGCTATTGAAATTATGAGTGTTGATCTGTTCTATAAATTGG2240.11794501866585229No Hit
AGCTTGGGGATAGTAGTCAAAAGGATAATTTAATAAGCATTTTACGTTACTA2230.11741847840395114No Hit
GTAAGGGGGCTCACTGCAAGCTCCACCTCTTGGGTTCACACCATTCTCCTGC2230.11741847840395114No Hit
CGTTTGGGGCAATATGCAACTGATCCCGCTTTTATTAATTTTTTGAAGAAGT2230.11741847840395114No Hit
CGACCCGGGACATCAATTTTCTTCCAAGATTTAATTTGGCACACAGGGTTTT2230.11741847840395114No Hit
GCCTTCGGGATATACGGTATCAGAGACTGGTGGTGGAGCTGCTTATCAAAAA2220.11689193814205003No Hit
GCGATGGGGTCGTAGGTATTAGTGCTAGTCTCAAATAGGTGCTTCCCCTGAG2210.11636539788014891No Hit
GCTATTGGGGTTGTACAGGGTGCCAGATAGATTGGAAAAAAGGAAAGAATGT2200.11583885761824778No Hit
GCATCCGGGGATGTGTGGATTAGTGAGGAAAAAGAAACAAAGGAAACTCAGT2190.11531231735634666No Hit
CAACTTGGGAACTCAAAATGGATTAAATATTTAAATGTAAGACCTCAAACTA2180.11478577709444554No Hit
GCTGACGGGCTAATTAACTTGATAAAGCTTTACTCTCATTATAAGCCCAGTT2160.11373269657064328No Hit
GTCAACGGGATTTTTAATAGATGAAGGAGGATTTGGGACTTCAAAAATGTAA2150.11320615630874215No Hit
AGTAGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGG2150.11320615630874215No Hit
AGTTGGGGGGTTCTTTGAATGGTTGCTGATGTTATTTAGCAATAATTCAAAT2150.11320615630874215No Hit
TCCATAGGGATGCATGGGATGGCAAAAGTGTGGTTCCAGAAAAGCCAAAGAA2140.11267961604684103No Hit
TACCACGGGGGTCTTCTGTTTAGTGTTGTATTTCAATTGTGGAGCCTCATTT2130.11215307578493991No Hit
GTCAATGGGCATCTGAGACATCCTCCTCGAAATGATACCAGGCAGCCAAGAA2120.11162653552303876No Hit
AACTAAGGGTACATCATTAACCTCACTCAGCCCTACTGCCCATCTATAAAGT2120.11162653552303876No Hit
GATAAAGGGATAGCACAAACTTATAACAAAATCCACTGTTAAATTTGGCAGT2110.11109999526113763No Hit
ATAGTGGGGATCCCCAGATCATTTAAAAGTTAACTAAAGTGAGAATGAAAAA2100.11057345499923651No Hit
GCTAGTGGGAGAACCAGGTTTTTGTTTTGATGATTTATCCAGTTGTTTTCTG2100.11057345499923651No Hit
GCTATGGGGACATATCTTGGTTTTTCTCCAAGCTTTCTGGCAACTTTTCTCA2090.1100469147373354No Hit
TTCTACGGGTATTACTGTGGTATTAGAATCTTTGGCATGGGAGATTGATGGC2090.1100469147373354No Hit
ACGTCAGGGGAGTTTCTACCAAGATCTCCACAATACTATGGTCAAATTAACA2080.10952037447543428No Hit
AAATCAGGGATAATTCTGGACCCCTATTTATCTCAAAACTCTTAATATATGC2080.10952037447543428No Hit
CACAGTGGGACAGACCAGCAGTCACAGAAATCATTCTTCCTGCTGTACTGGG2080.10952037447543428No Hit
GTCAAAGGGGTGTCTAATGTTCTTCAAAAAAGTAATATCCTCACTTGGAGAG2060.108467293951632No Hit
ATCTAGGGGTCCAGGAACGTGTCCCTACCTGGAATCCCCAGGGGGGCTTCCA2050.10794075368973088No Hit
TACTATGGGAATGTCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGG2050.10794075368973088No Hit
TTTCTGGGATTCAATAAATGGTGTTGGGATAACTGGCTAGCCCTATGCAGAA2050.10794075368973088No Hit
CGACCAGGGGCTTCTCACTCACTCAGGACACTTCTTGAGGCAATGTAGACAC2040.10741421342782977No Hit
TCTCAAGGGCAGAAGATGATGGATTCCCTCTTCTTAAAATTGGATGCCCTCT2040.10741421342782977No Hit
TGAGGGGGCATTGTTGATATTTTAGTAGCTTTCATTATCTGCTACAGCCAGA2040.10741421342782977No Hit
TCATTAGGGAGAACATTGTGATCTATGGTATTGAAAAGCAGGAATAAAGAGA2030.10688767316592863No Hit
TATTCAGGGGCTCGCGGGCGTGTGCGCGCCGCAGGCTGGCGGTAAGGCTGGA2030.10688767316592863No Hit
TACGGGGAGGCAAGATAATTTAATCAAAAGAAATTAAGAAAATTTTCCAAAA2020.10636113290402749No Hit
CTCACAGGGTATGGTTTCATTTTGGTTGTACAGTATATAAACCATATTTGTT2010.10583459264212637No Hit
AGACATGGGACTGGCCAATGCTTCACCAGTTAGTCATGCTCCAAGGATTGAG2010.10583459264212637No Hit
ATTGTTGGGTTAGAAACAAAAACCATGTTGGAAGACCAACTAGAGGGAACTC2000.10530805238022525No Hit
TATCACGGGACGCAGATGTAATGCACATTATTGGCGTATCTTTAAGTTGGAT2000.10530805238022525No Hit
TAGTTAGGGTATATCATTAAATGTTAAGCTTGCTAAAGCAGTATTTTTTAGG2000.10530805238022525No Hit
TATGCAGGTGACTACCATCTCTTCTGCGCGCTGCTCACTGGCTTCCTCTTCG2000.10530805238022525No Hit
CAGTCTGGGACTTCATTTTATAATGAACAGTTACATGAAGAGTGCCTGCTCC1990.10478151211832414No Hit
TTATATGGGGATCTTGATTAGGGATAAAGGGCCAGATAGTAAATATTTTAGA1980.104254971856423No Hit
GACTAGCGGGCTGGCAAAATGTGTTTACGTATCTGACAAGTTGTACATTTGT1970.10372843159452189No Hit
CCACCTGGGATTCCAGAAAGGCCATGTACAAGAGGAAGTACTCAGCCGCTAA1970.10372843159452189No Hit
TATCGCGGGACTTGGATAACTGGCTAATCATATTAAAGGACTATGTGGTTCC1970.10372843159452189No Hit
ACTTCTGGGATACATTTATGCACATTCACGCTGTTTGTTTCATATATACAGG1970.10372843159452189No Hit
CTTGATGGGAACCTGACAGTGAACTATAATGCAGAAAAAGTGATCTCCTATG1960.10320189133262074No Hit
CCATCCGGGTCTCTTGACCTCGTGATCCGCCCGCCTTGGCCTCCCAAAGTGC1950.10267535107071962No Hit
GTACCAGGGAAGTACCTCTGACTTTGCTTTCTCAAATCACTGTTTCTAGCCC1950.10267535107071962No Hit
TCTATGGGGTACTATTTTCCCTCAGTAACATGTAATTGCTACATTTTTTATA1950.10267535107071962No Hit
TAAAGTGGGAGGATGACAGACACTGTGTAACAACACCTGGGTCAACTCTTTT1940.10214881080881849No Hit
TTCACAGGGGAAATGATGAGATGAGATGAGATGATGAGATGAAATTTTGAGA1940.10214881080881849No Hit
CACCGTGGGGCAATGATTTTATCCCCCAGAATCCTTCACCTTTCAAGGAGTT1940.10214881080881849No Hit
AAACGGGGGTGGAAAAACTATCTTGGGATTCTTTCATACATTTTCATCAAAA1930.10162227054691737No Hit
GTGCCGGGATATGTACCACATTTCATTTTCTTTTTCCAATCCGTCATTGATG1930.10162227054691737No Hit
AAAGCTGGGCTGCCAAAATTATGGATCCGGATGAGCAATTGGAAACCCTTCA1930.10162227054691737No Hit
AGGGTTGGGAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATG1920.10109573028501626No Hit
GTAGAGGGGAACAGGGACTGTTTGTCAGTATTGCTGTCATCCCCAAAAGAAA1920.10109573028501626No Hit
TGCTGAGGGCGATCTGGTTATGGGCACTGAGATTCAGGTTTAGGAAGCTGAC1910.10056919002311511No Hit
CTCTAAGGGGAATGGGGAGAAGGTTATGAGAAGAGCATTATTAAGTTTCCAA1910.10056919002311511No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)