FastQCFastQC Report
Sat 31 Dec 2022
SRR5848367.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848367.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190845
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGCCGTCT13510.7079043202598968TruSeq Adapter, Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCTGTGATCTCGTATGC10060.5271293458041867No Hit
GTCAACGGGATTTTTAATAGATGAAGGAGGATTTGGGACTTCAAAAATGTAA2500.1309963583012392No Hit
TCTGGAGGGACAGATAGAATTTTTATTAAGTGGTTTGTTGAAAGTTTGTCAG2440.1278524457020095No Hit
CTTGATGGGAACCTGACAGTGAACTATAATGCAGAAAAAGTGATCTCCTATG2370.12418454766957479No Hit
AGTCAGGGGGCAGATACCACATTAATCAAGTGAAAAACATTAATAACATCGT2370.12418454766957479No Hit
GTCAATGGGACACAAACATGTCTGCTAATGAACTGATGATATGTTAAATAAT2370.12418454766957479No Hit
TAGAGCGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT2360.12366056223636983No Hit
CGTTTGGGGCAATATGCAACTGATCCCGCTTTTATTAATTTTTTGAAGAAGT2350.12313657680316488No Hit
TCAGTCGGGATGTCCCCATCGTGCTTTGTGCACACTCCTTTTTTGTATGTTT2350.12313657680316488No Hit
CATATGGGCAGTAGGTTAAAAATATTTTAATCAACTGACTGCTAAACACATC2310.12104063507034504No Hit
TACCACGGGGGTCTTCTGTTTAGTGTTGTATTTCAATTGTGGAGCCTCATTT2270.1189446933375252No Hit
GCTATGGGGACATATCTTGGTTTTTCTCCAAGCTTTCTGGCAACTTTTCTCA2260.11842070790432026No Hit
CATTGCGGGATGGCTATTGAAATTATGAGTGTTGATCTGTTCTATAAATTGG2260.11842070790432026No Hit
TACTATGGGAATGTCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGG2250.11789672247111531No Hit
CCGTCGGGATGTCACCCCAAACCACTTTGAGGAACAAAGTTTCAATAACTTC2230.11684875160470538No Hit
ACGTCAGGGGAGTTTCTACCAAGATCTCCACAATACTATGGTCAAATTAACA2200.11527679530509052No Hit
GCTAGTGGGAGAACCAGGTTTTTGTTTTGATGATTTATCCAGTTGTTTTCTG2190.11475280987188556No Hit
AAAGAAGGGCCTTTACTGCATTCTGTGCGATTGTTTTTCTAATTTAGCTCTT2190.11475280987188556No Hit
TTCTACGGGTATTACTGTGGTATTAGAATCTTTGGCATGGGAGATTGATGGC2170.11370483900547564No Hit
GCGATGGGGTCGTAGGTATTAGTGCTAGTCTCAAATAGGTGCTTCCCCTGAG2170.11370483900547564No Hit
GCTGACGGGCTAATTAACTTGATAAAGCTTTACTCTCATTATAAGCCCAGTT2160.11318085357227069No Hit
AAATCAGGGATAATTCTGGACCCCTATTTATCTCAAAACTCTTAATATATGC2160.11318085357227069No Hit
GCATCCGGGGATGTGTGGATTAGTGAGGAAAAAGAAACAAAGGAAACTCAGT2150.11265686813906574No Hit
CTAGACGGGCGATCTGCATTGGGAGGATATGGTGGTACCAGGAGACGATAAA2130.11160889727265583No Hit
CGACCCGGGACATCAATTTTCTTCCAAGATTTAATTTGGCACACAGGGTTTT2130.11160889727265583No Hit
GCAGAGGGGTCACGAGAAAGATATAAATTTTGTTATTTTAAGCCATTAAGGT2120.11108491183945088No Hit
CAACTTGGGAACTCAAAATGGATTAAATATTTAAATGTAAGACCTCAAACTA2120.11108491183945088No Hit
CACAGTGGGACAGACCAGCAGTCACAGAAATCATTCTTCCTGCTGTACTGGG2110.1105609264062459No Hit
GCCATAGGGAGCCCAGGCAATTCAGAAATTTATCAGATAAATTTAACAAAGA2100.11003694097304094No Hit
TTGTATGGGACCACTTTTTGTATTTGTCATGATATTTTTGACCCATTGGCAA2090.10951295553983599No Hit
TATCACGGGACGCAGATGTAATGCACATTATTGGCGTATCTTTAAGTTGGAT2090.10951295553983599No Hit
GACTAGCGGGCTGGCAAAATGTGTTTACGTATCTGACAAGTTGTACATTTGT2090.10951295553983599No Hit
AGTAGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGG2080.10898897010663104No Hit
CACCGTGGGGCAATGATTTTATCCCCCAGAATCCTTCACCTTTCAAGGAGTT2070.10846498467342608No Hit
TGAGGGGGCATTGTTGATATTTTAGTAGCTTTCATTATCTGCTACAGCCAGA2060.10794099924022112No Hit
TAGTTAGGGTATATCATTAAATGTTAAGCTTGCTAAAGCAGTATTTTTTAGG2060.10794099924022112No Hit
CGAAACGGGACCTTTTAAAGAACAGAAAAATGAGCAACTGAAATTTTGAGAA2060.10794099924022112No Hit
ACCGCGGGGATGTCACCCCAAACCACTTTGAGGAACAAAGTTTCAATAACTT2050.10741701380701615No Hit
AATAGCGGGCATATAAATTACACAAATCAAATCTGTCGACATCCCAAGAATA2040.1068930283738112No Hit
GGCGCTGGGAGTCCTTCTGGTCAGTGAACGAAAATTCTAGACCTACAGTTAC2040.1068930283738112No Hit
GTAAGGGGGCTCACTGCAAGCTCCACCTCTTGGGTTCACACCATTCTCCTGC2040.1068930283738112No Hit
AGTTGGGGGGTTCTTTGAATGGTTGCTGATGTTATTTAGCAATAATTCAAAT2020.1058450575074013No Hit
TGATATGGGAGAAAGGGATGGATGAGAAGACAGACCTGAGACCAATCTGGGT2010.10532107207419635No Hit
TAAAGTGGGAGGATGACAGACACTGTGTAACAACACCTGGGTCAACTCTTTT2000.10479708664099137No Hit
AGCTTGGGGATAGTAGTCAAAAGGATAATTTAATAAGCATTTTACGTTACTA2000.10479708664099137No Hit
TGCTGAGGGCGATCTGGTTATGGGCACTGAGATTCAGGTTTAGGAAGCTGAC2000.10479708664099137No Hit
AAAACCGGGATAATAATTTTAATTCAATCAACTGAATAATCAGGTTGGCTCT2000.10479708664099137No Hit
TATGCAGGTGACTACCATCTCTTCTGCGCGCTGCTCACTGGCTTCCTCTTCG1990.10427310120778642No Hit
CTTCAAGGGAACATAAAAAAGTCTCTTACACCTCACATTTCTTGTGTGACAA1990.10427310120778642No Hit
GCCTTCGGGATATACGGTATCAGAGACTGGTGGTGGAGCTGCTTATCAAAAA1980.10374911577458147No Hit
TCATTAGGGAGAACATTGTGATCTATGGTATTGAAAAGCAGGAATAAAGAGA1980.10374911577458147No Hit
CCATCCGGGTCTCTTGACCTCGTGATCCGCCCGCCTTGGCCTCCCAAAGTGC1980.10374911577458147No Hit
CGCTGGGGGTATGCAGTGTGATCTTTATTTATAGTAAATTATGTTTCATGTA1970.10322513034137651No Hit
CATTATGGGATTTCTGCTTTGGGATCTGGAAGTGTTCCTCTGCTCGCCTCAT1970.10322513034137651No Hit
GTGATGGGGAATTAAACATATCAATTTCAGAATGATTAATGGAGCCACAGAT1970.10322513034137651No Hit
TATCGCGGGACTTGGATAACTGGCTAATCATATTAAAGGACTATGTGGTTCC1960.10270114490817156No Hit
CAGTCTGGGACTTCATTTTATAATGAACAGTTACATGAAGAGTGCCTGCTCC1960.10270114490817156No Hit
GCACTGGGGATAGCTAGGTGGGGCCAGATGATTTGAAAATTAACATATTGTT1960.10270114490817156No Hit
ATTGTTGGGTTAGAAACAAAAACCATGTTGGAAGACCAACTAGAGGGAACTC1960.10270114490817156No Hit
ATCTAGGGGTCCAGGAACGTGTCCCTACCTGGAATCCCCAGGGGGGCTTCCA1950.10217715947496661No Hit
TACGGGGAGGCAAGATAATTTAATCAAAAGAAATTAAGAAAATTTTCCAAAA1940.10165317404176163No Hit
TTATATGGGGATCTTGATTAGGGATAAAGGGCCAGATAGTAAATATTTTAGA1940.10165317404176163No Hit
CTTACCGGGCTTGCAGGATGGATAAATGCGTTTTCTCTTCCTTGCCGCAAGG1940.10165317404176163No Hit
GTGCCGGGATATGTACCACATTTCATTTTCTTTTTCCAATCCGTCATTGATG1930.10112918860855667No Hit
GTAGAGGGGAACAGGGACTGTTTGTCAGTATTGCTGTCATCCCCAAAAGAAA1930.10112918860855667No Hit
TGCAAGGGGGAAATAAGCATAATTGCTAATACTAAGATTTAGCAATATTGTA1930.10112918860855667No Hit
TTATAAGGGAAGAATTTAATATTTATTTCACTTTGAATGGACAGCCTTAAGA1920.10060520317535172No Hit
CAATACGGGATAATTACAAATATCCGAAAGTCATTTCTTGGAACACAAGTGG1920.10060520317535172No Hit
TTTCTGGGATTCAATAAATGGTGTTGGGATAACTGGCTAGCCCTATGCAGAA1920.10060520317535172No Hit
GACTCCGGGCAGCACACATCTCTGTGATGTAGGTACTACCTTATGCTCACTT1920.10060520317535172No Hit
TGCAGGGATGTAGTATTCTTGCCAAAAATATTTAACTGAATCTAATCATGAG1920.10060520317535172No Hit
AGGCTAGGGAGGCGCCCGCCACCAAGTCCGGTTAATTTTTAGTATTTTTTTT1910.10008121774214677No Hit
TTGCCAGGGCAGGGTTCTCTCTCCTGAGCCTAGATTTGCCAGGGTCTTGGTG1910.10008121774214677No Hit
ACTCTAGGGCTAGACAAGGGTTCTTTAAAGAAAGACAGCGCAACTTTGAATG1910.10008121774214677No Hit
CGACCAGGGGCTTCTCACTCACTCAGGACACTTCTTGAGGCAATGTAGACAC1910.10008121774214677No Hit
TACTTGGGGTACACAGGTACTGACACACAATATGTAGGCAAGGTAAGAATTT1910.10008121774214677No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)