FastQCFastQC Report
Sat 31 Dec 2022
SRR5848330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306733
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGC17140.5587921743014282No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGCCGTCT16890.5506417633577085RNA PCR Primer, Index 32 (95% over 21bp)
TGCTCCGGGGGGCCAGCTGCAGACACTAGAACAATATTGAATCAGTAACTTT4690.15290170930418312No Hit
GTAAAAGGGTAAATTGCAAACTCTGAACTTTTTACAAAGAAAAATGGAAAAA4650.15159764355318794No Hit
TCTGTAGGGGAAAGAGCAAAATAGTTTATCACTACTAGAAGCAAGAGAAGCA4370.1424691832962218No Hit
ACAAATGGGCCAGCCATCCCAGTTTACTAGATAACGTATTTTTCTACCTGAT4350.1418171504207242No Hit
ATTAGCGGGATACAAAGGATGTACTATTATTAACCCCATTTTATAGATAAAG4310.14051308466972906No Hit
GTATGTGGGCTTGGTCGTCTTTCAAGAAACAATGGCTCCATCATTCCTACAT4310.14051308466972906No Hit
ACGCCTGGGTGAAACTATAAGTCTTTGAGGAAAATTATAAGGTAAAATTTTC4270.13920901891873388No Hit
GGTTCCGGGAGTAATGTGAAGCATCTTTTTGTGTGATTGTTGGCTTTTATAT4220.13757893672998994No Hit
TTACATGGGTCACTTTCAGCAGTCCGGGTAAAATCTGTGGATCAGGGTTAAA4070.13268869016375806No Hit
CAAATCGGGATAACGAGAGCAGCTTAATAGAGCAAGCTCCAATACCTCAGGA4070.13268869016375806No Hit
ACGTCCGGGATACATACCTGGCTCCAAAACAAGGCTGTATCTTCTGCCACTG4040.13171064085051168No Hit
GCTTGTGGGAATGATTTCCTAGATCACTTTAACTTTTTTGTGTCATGGACTC4040.13171064085051168No Hit
CTATCCGGGACCTGGATGCCTACGTGGGAAGGGACCTCGAATGTGGGACCCC4000.13040657509951653No Hit
CGCAATGGGGATTGAAACTGTGTCAATTTTGTAGAAAAAGATATTCTGGGGT3990.13008055866176774No Hit
TCAAATGGGGATGGAATATTGTGATTGACAGCCCCCACTTGCTTGCAATGAC3960.12910250934852135No Hit
AGCTTTGGGTCAGCCCAAAATCTCCTTAAGCTGATAAGCAGCTTCAACAAAG3910.12747242715977739No Hit
TGTCTCGGGACTAGAGTAGCAGGTGGTGTTGGAATTACCTGTTGGATTTTAG3900.1271464107220286No Hit
TGGAGTGGGGAGTCATCCAGTCCTGGGCACTGAGGTCTGTTAGATGAAAGGA3870.12616836140878224No Hit
TGCTCTGGGATTGAAAAACTGAATCATTTAAAATCACAAAAGAGAAAATCAA3840.12519031209553586No Hit
AACTTTGGGGATATATAATTATATATGTGGCATTCCTGCCAAAATGTTTAAC3830.12486429565778706No Hit
GTGCCAGGGAATGAAGCCCAGTTGAGTTGCAGAAAATTGACCACTTTTTCAT3830.12486429565778706No Hit
TCGAGAGGGAACTTTCAACTCATAACAAATGAATACTACTATCCAGAATATG3810.12421226278228949No Hit
TAACGCGGGACTGGAATGGAGGAGAAGGGGCAGTCACTGGTGTTAATAAAAT3810.12421226278228949No Hit
GCTCACGGGAATCTGGGAGAAGTACCATTTGATTTATTGCTATACATTTCAC3800.1238862463445407No Hit
GTCTCTGGGGACATTGTGGACATCGGATACCCAAGGAGACGAAGCTGAAGCA3790.1235602299067919No Hit
GCATGCGGGGTGGAAAGTTGCCATTTTTATGGGTTTATAGGATGAAAAAATT3790.1235602299067919No Hit
ATTTGCGGGGTATGGATTTCTTCTGGCAGATCTGCATGAGCTCTCAGTGATG3770.12290819703129433No Hit
TCTGGCGGGATTTCTATCTTGCTCAAATATATTGGTTGGCATTCTTTTGTAA3750.12225616415579674No Hit
ATAGGTGGGGAGGAGCTACGGCCGGAAGATGGCGGCGGCCGCAGAGTTGTCG3740.12193014771804794No Hit
TTGCGTGGGACGCCAGGAATAGAATTCATCAAAATTTAATTAATTTAAATGA3730.12160413128029916No Hit
AGCTAAGGGGAAGAATAATAATTCTACAAAAGTTCTGTACTTGAAAGAGATT3720.12127811484255037No Hit
CAGAGTGGGGAAGGTCCTTTCTGGAAACAGGATTACAAACATATGGACCTAT3710.12095209840480158No Hit
GATGCTGGGTCACCCAGTGTTCTTTGGATGTCCCTTCCCTAGGGGGAAAGGC3700.12062608196705278No Hit
TCGCTAGGGACCAAGTCCTTGGTAGATATGGCTCCTCCCTGCTTCCCTCTTT3640.11866998334056002No Hit
GACAATGGGAACTGTAATTGCCTGGGAAAGTATTGGAGATGTGGATGGGGGT3620.11801795046506244No Hit
TGATTTGGGATCCTTGATAATAAATAAAATAATCTATGTTACCTATTGTGTT3610.11769193402731365No Hit
TAGTCGGGTACTTTCCAGCAGTCAGTCCCTGGTTCCTAGATGTGTTCTAAGC3610.11769193402731365No Hit
GCACTAGGGAATGATATTTATTGAATGAACAGGAAACAGGGAAAGACATTGA3580.11671388471406728No Hit
TTTAGTGGGGAGGAAGGATTAGAAGATATTGACGAAGAAGGGGATGAGGATG3570.11638786827631849No Hit
CTGGCGGGCTGGAAAGATAATATTACTGGTTGCAAATGCAGTTAAACCTATT3560.1160618518385697No Hit
TTTGGTGGGCTTCTTATACTTTTTAATGATTTTGTAGATAACCAGGCAAATC3550.11573583540082091No Hit
AACATCGGGAACAGAAGCCTAGCATAACAATGAAATTAAGAAATAATTGTTA3550.11573583540082091No Hit
AACCATGGGGAAGTAGAAAGAAATATAAATCATTCAAGCCAATAGCTGGATT3550.11573583540082091No Hit
AGACGGGGGGGCCGTGGCCCGGCTGGGTCGGCTGAAGAACTGCGGATGGAAG3530.11508380252532333No Hit
ATAAGTGGGATAACAGCTTATAGAACTTGAACTACTAAATATGAAAATAAGT3520.11475778608757453No Hit
GTTGCGGGATATATGAAAGTTCAAACAGAAATTGCATTGTTATTACAGAGAA3500.11410575321207696No Hit
ATGCTTGGGACATTTTAAAATATGCTTAAAATTCAGACCTTTTACATAAAAC3490.11377973677432816No Hit
GTACTAGGGATCACTCCAAATTACCTCTTAAAGATTTTCTCATTTTTTTTTT3490.11377973677432816No Hit
TCAATAGGGTGACTTAGGAGTTGTATTTCTCAATGTACCTGAAAATCTCAAA3480.11345372033657937No Hit
TATTATGGGATTGATAAATATTACAAAACTGAATGCAAATGTCCTTTGTGGT3470.11312770389883059No Hit
TTATCCGGGACCTAATGATTATTTCTATAACATTAAGCATGGTAATAAGTAG3460.1128016874610818No Hit
AGTAGTTGGGGAAGCCGGAATTCTTTTAGGCAATTTAAACTTTCTGAAATAG3450.112475671023333No Hit
TTCTGCTCGGGGATACATAAATAAGAATATATTTGGTTCCAAGCTTTTGTCA3440.11214965458558421No Hit
CGTAGAGGGTACCTTGTCTCTTTTTCATGCTGAATAATATTCTGTGGTATGG3430.11182363814783543No Hit
TTAAAGGGAGATGTGGAAGATGTGAAATTTGTCATCAATTATGACTACCCTA3410.11117160527233784No Hit
CTTCACGGGGAAGGGTCGATTTTTTTGAGGTTTCTGTAAAATGAGATAAAAG3410.11117160527233784No Hit
ATTGTTGGGAACAGGCAAAATATAAATTTCTTTGTTTCATGTATGACTCATC3400.11084558883458905No Hit
GTGGCGGGTGCTCAGTGGATCCACAGTGCCTTCAGGAACTGTGAAGTTAACT3400.11084558883458905No Hit
GTACCTGGGTCTCATTTATTTTTACTTTATGGCATTAAAATACAATAAATCT3390.11051957239684027No Hit
TGTCCCCCCCCAGTTGATTCTGATGTACAGCCAGGTTTGGGAACTGGCCTTC3390.11051957239684027No Hit
TTGATAGGGATTAAAGCAAAACTTAAAAAAATGTTGACGTCTGTTAAATCTA3380.11019355595909144No Hit
GCCACTGGGCAGAAAGCACTTTGGTCTTAATTGGTGACTTGGGGGTTATTTG3360.10954152308359387No Hit
GTCAAGGGGCATTGTTGAAAGAAATTAAAGAAGACCTAAATAAATGGAAAGA3360.10954152308359387No Hit
TTATATGGGGGAGGCGCTAAAGCAAAAACGATTTCCACCAGCAACAGAAAAC3350.10921550664584508No Hit
CATATAGGGACTTAGTCACGGGGATGGTGGAAGAGGGAGAAGAGGAAGGGTG3350.10921550664584508No Hit
ACGACCAGGGTGCTTTTTTTATGCAAATAACCGATTAGGTCAGGGGTCGATC3350.10921550664584508No Hit
GTCTAGGGGAAGGGGGTTGTCCTTATTATGAATAGATTGTTACTTTTCCAAT3330.1085634737703475No Hit
AGCGGCGGGAAAGTGGTACAAGATTTTATTGATATATTTTCTGATTATGCTA3330.1085634737703475No Hit
GTGGATGGGATAAGCAAATAGAAATCTCATGCTATTTCTCATTATTATTTTC3320.10823745733259871No Hit
TAAGTCGGGGATTTCCACCAGCAACAGAAAACTTCCTTTATCATCTAGCAGC3320.10823745733259871No Hit
GCTGCCGGGGTATTCCGTCAAGTAAGATAATAACAGCTGACCTGCCAACAGC3320.10823745733259871No Hit
CAATGGGGATGGCTGTGGTTTCAATGTTTACTCTACCTGTAGTGTATGTTAA3320.10823745733259871No Hit
AGTCCCGGGGATCTTGGAACGGGAAGATGTGAATGTAATCATCGATTTGCAT3320.10823745733259871No Hit
GCACTTGGGGAAGAGAAGCAATTTTGGGGAAGGGTTTGAATTGTTTTCTTTA3320.10823745733259871No Hit
TGTTTTGGGTCTGTGAAGAAACTTTGCTGAGTTAACCATAAATGTTCATTCA3300.10758542445710112No Hit
CTGACAGGAGTAGGGAGGACTGTTGAATAAGCCAATAGAGTCTTAGAAACTA3300.10758542445710112No Hit
TAAAGGGGCCAATCATATTGGGAAAGTGCTAGCTGTGCTCACCTTCATGGGG3300.10758542445710112No Hit
TTTGCAGGGATACTTAATTTTATTTATGTATAGAATATTTGTATTTATTTTT3300.10758542445710112No Hit
TGTTTTGGGAATGATAGATAATGGAGATACAGAATGACTCATTGGGTTCATA3300.10758542445710112No Hit
CATTGCGGGGGAGGGGAGAGGAATAAAGTTTTATGGGACACCTCCTTACTTC3300.10758542445710112No Hit
TTCGGTGGGGCTTGATAATAATAGCTCTTTGCCAGTAAGCTTTACTTTGTTG3290.10725940801935233No Hit
GCAGGGCTATACAAAATTATTTTTATCTTCTGTTATAATTAAATACAATCAT3290.10725940801935233No Hit
CAGATAGGGAAGTATGCAGACAACCAAATGAAGGCCTTTCAGCAGGCGAATC3280.10693339158160355No Hit
CGCGCTGGGACATGCACAAGTTTTGCAGATTGCCAAGGAAGTTGCCCTCTGG3280.10693339158160355No Hit
GTGATGGGGGGTGTATGGACTGGAAAAATGCATGCGAGATGTTCCTTTGCTT3270.10660737514385475No Hit
AAGCCGTGGGTACCAAGCACACTGCCTACCTCAGGCCGTTTGAACTTGCTAT3270.10660737514385475No Hit
CAGCAGGGGAATTAATAAACGCACAGCCCTATGTGAACAGACAGGAATTTCT3250.10595534226835718No Hit
CCGTTTGGGATGAATATTGCATCTCTAATCTAATCTAAAAATGTTGTTTTGA3240.10562932583060838No Hit
AACGTCGGGGGAACAAAGAACATATAGTACCAACAACGAGATGGTAGACAAA3240.10562932583060838No Hit
TACCTTGGGAGTAAATCCTTTTAGGGGAATCTGAAATGCCTTAATATTAACT3230.10530330939285959No Hit
CACGCTGGGAGGAGCAAATCCAGGCAGAGAGGGAAGTTACTGAGACCCAGAA3230.10530330939285959No Hit
ATCTACGGGAGTGTTCTCTATCTGAACCAGCTTTTCTTCTCAGTGAAGTTTT3230.10530330939285959No Hit
TACCAGGGTCCTGGCAGGGGCCCCCCGGTTTGCCTCCCTAGAGCCTGACCAG3230.10530330939285959No Hit
CAAGCTGGGCTATTTTCACAGTTTATTAAACTGCCTAACACACATGATTTCT3210.104651276517362No Hit
GTTACAGGGCTCCCAAGTAGCTGGGACTACAGGCACGTGCCACCACGCCCAG3210.104651276517362No Hit
ACTTCAGGGATGGCTTGATTTTCTAGTCTAAACCTCTGGTCTCACACTTCTC3200.10432526007961322No Hit
CACAGCGGGAATCCAAAAACACTCCATGTAATACATAAGTTTCCACTGGATC3190.10399924364186443No Hit
TCAACTGGGTTGGAGTACAAATTAGACTTATAGTCCTCCTGGAACTTGAGTT3190.10399924364186443No Hit
GGGGCACTTTTAAAAAAATAATTGGTTGACTTAATATATTTGCTTTTTTTCT3180.10367322720411563No Hit
GGTTACGGGTGCCACCTGTGTCACAGTATGTAAACTTCAAGGATAGGAAATA3180.10367322720411563No Hit
CCTAGTGGGAAGGCCTGTATGTAAATGTTTATAGTTGATTCATCCATAAGAG3180.10367322720411563No Hit
CTCAATGGGACATGCTGGTCCTTCTGTTTGAAAGACAATTCCTCTTTGTCAT3170.10334721076636685No Hit
ATGCTTGGGGAGGAACATTTTATTGACATAGAAGATACACCAGGATATCAGA3170.10334721076636685No Hit
GATGTGGGGAGCATTAATAGAAACAGAGGTGCAACTAACATCTCTTGGTGAA3170.10334721076636685No Hit
GAAGCTGGGACCAACACGTCACCGGAGAGATGATGGGTGGCCATGCCATCCG3160.10302119432861806No Hit
GGTTTCGGGACAATCGAGTATTTTAGAAATTACATGAAACATGAAACAGTTT3160.10302119432861806No Hit
GAACAGGGGAGGCAAGAAGTAGAATGTCATCTTTCACTCTAATGCTTTTTAT3150.10269517789086927No Hit
GTGCTTGGGACACCTTTTACGATGACTACTTTTGGTGGAGTTGAAATGCTGT3140.10236916145312047No Hit
TCATATGGGGATCTTAGGCCGGGGAGAGTGACCGCGGCTGCTCCAGCGCTGA3140.10236916145312047No Hit
TTGGTTGGGGAGTGTGCGTGCGCTTAATATCAGAGTTTCAAAGGACATGCAG3130.10204314501537168No Hit
CGCGGGGGAATGTAAAATCTGTGGTAAGAAAACAACTATTCATTATTCAGTA3130.10204314501537168No Hit
GTGCACGGGTATGAATAATCTCAATTATATTCATCACACTTTTTTCATACCA3120.1017171285776229No Hit
TTCTATGGGCTAATTATCTTGTAAAATTTTCTTTTAAAAAAAGTATTAGGAG3090.1007390792643765No Hit
CTCTCAGGGCACTTGCCACAGATGGAGCCCGTGACGGTGTGGAGGGTTTCCT3090.1007390792643765No Hit
AATTTTGGGGAAAAGGAAAAGGCCAGCCTGCCAGGAGTGAAGAAAGCTTTGG3080.10041306282662771No Hit
TGAATCGGGGGTGGGAACACGAAACAGGAGTCTCTTCTGTTCTGCAAGTTTG3070.10008704638887891No Hit
CACGCTGGGGCTATGTTTAGTCTCTCTGGTTTTTAAGCAGGCTTACCCCTCA3070.10008704638887891No Hit
CACTAAGGGATTTATGGATGAGGAAGTTCAAAGCATACTCACGAAAATGACA3070.10008704638887891No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)