FastQCFastQC Report
Sat 31 Dec 2022
SRR5848329.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848329.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306211
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGCCGTCT16160.5277406755472533RNA PCR Primer, Index 32 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGC15970.5215358037431705No Hit
CGCAATGGGGATTGAAACTGTGTCAATTTTGTAGAAAAAGATATTCTGGGGT4720.1541420785014255No Hit
GTAAAAGGGTAAATTGCAAACTCTGAACTTTTTACAAAGAAAAATGGAAAAA4640.15152950089970643No Hit
ACGCCTGGGTGAAACTATAAGTCTTTGAGGAAAATTATAAGGTAAAATTTTC4500.14695749009669803No Hit
GCTTGTGGGAATGATTTCCTAGATCACTTTAACTTTTTTGTGTCATGGACTC4380.1430386236941194No Hit
TGCTCCGGGGGGCCAGCTGCAGACACTAGAACAATATTGAATCAGTAACTTT4330.14140576269304497No Hit
GTATGTGGGCTTGGTCGTCTTTCAAGAAACAATGGCTCCATCATTCCTACAT4310.1407526182926152No Hit
TGCTCTGGGATTGAAAAACTGAATCATTTAAAATCACAAAAGAGAAAATCAA4260.1391197572915408No Hit
ATTAGCGGGATACAAAGGATGTACTATTATTAACCCCATTTTATAGATAAAG4250.1387931850913259No Hit
CAGAGTGGGGAAGGTCCTTTCTGGAAACAGGATTACAAACATATGGACCTAT4200.13716032409025147No Hit
GTCTCTGGGGACATTGTGGACATCGGATACCCAAGGAGACGAAGCTGAAGCA4190.1368337518900366No Hit
TTACATGGGTCACTTTCAGCAGTCCGGGTAAAATCTGTGGATCAGGGTTAAA4150.13552746308917707No Hit
ATTTGCGGGGTATGGATTTCTTCTGGCAGATCTGCATGAGCTCTCAGTGATG4140.13520089088896217No Hit
ACAAATGGGCCAGCCATCCCAGTTTACTAGATAACGTATTTTTCTACCTGAT4120.1345477464885324No Hit
TCGAGAGGGAACTTTCAACTCATAACAAATGAATACTACTATCCAGAATATG4060.1325883132872431No Hit
CAAATCGGGATAACGAGAGCAGCTTAATAGAGCAAGCTCCAATACCTCAGGA4060.1325883132872431No Hit
CTGGCGGGCTGGAAAGATAATATTACTGGTTGCAAATGCAGTTAAACCTATT4030.13160859668659847No Hit
AGCTAAGGGGAAGAATAATAATTCTACAAAAGTTCTGTACTTGAAAGAGATT4000.1306288800859538No Hit
GTGCCAGGGAATGAAGCCCAGTTGAGTTGCAGAAAATTGACCACTTTTTCAT4000.1306288800859538No Hit
GTACTAGGGATCACTCCAAATTACCTCTTAAAGATTTTCTCATTTTTTTTTT4000.1306288800859538No Hit
AGCTTTGGGTCAGCCCAAAATCTCCTTAAGCTGATAAGCAGCTTCAACAAAG4000.1306288800859538No Hit
CACTAAGGGATTTATGGATGAGGAAGTTCAAAGCATACTCACGAAAATGACA3970.12964916348530914No Hit
ACGTCCGGGATACATACCTGGCTCCAAAACAAGGCTGTATCTTCTGCCACTG3940.1286694468846645No Hit
TCAAATGGGGATGGAATATTGTGATTGACAGCCCCCACTTGCTTGCAATGAC3870.1263834414831603No Hit
TGTCTCGGGACTAGAGTAGCAGGTGGTGTTGGAATTACCTGTTGGATTTTAG3860.12605686928294543No Hit
TTAAAGGGAGATGTGGAAGATGTGAAATTTGTCATCAATTATGACTACCCTA3860.12605686928294543No Hit
GCTCACGGGAATCTGGGAGAAGTACCATTTGATTTATTGCTATACATTTCAC3850.12573029708273054No Hit
ATTGTTGGGAACAGGCAAAATATAAATTTCTTTGTTTCATGTATGACTCATC3800.1240974360816561No Hit
ATAAGTGGGATAACAGCTTATAGAACTTGAACTACTAAATATGAAAATAAGT3800.1240974360816561No Hit
GCATGCGGGGTGGAAAGTTGCCATTTTTATGGGTTTATAGGATGAAAAAATT3770.12311771948101147No Hit
TCTGGCGGGATTTCTATCTTGCTCAAATATATTGGTTGGCATTCTTTTGTAA3760.12279114728079657No Hit
TGGAGTGGGGAGTCATCCAGTCCTGGGCACTGAGGTCTGTTAGATGAAAGGA3750.1224645750805817No Hit
CAGATAGGGAAGTATGCAGACAACCAAATGAAGGCCTTTCAGCAGGCGAATC3740.1221380028803668No Hit
AACTTTGGGGATATATAATTATATATGTGGCATTCCTGCCAAAATGTTTAAC3730.12181143068015193No Hit
ATAGGTGGGGAGGAGCTACGGCCGGAAGATGGCGGCGGCCGCAGAGTTGTCG3720.12148485847993704No Hit
TCTGTAGGGGAAAGAGCAAAATAGTTTATCACTACTAGAAGCAAGAGAAGCA3700.12083171407950727No Hit
TATTATGGGATTGATAAATATTACAAAACTGAATGCAAATGTCCTTTGTGGT3700.12083171407950727No Hit
AGACGGGGGGGCCGTGGCCCGGCTGGGTCGGCTGAAGAACTGCGGATGGAAG3700.12083171407950727No Hit
CTATCCGGGACCTGGATGCCTACGTGGGAAGGGACCTCGAATGTGGGACCCC3700.12083171407950727No Hit
TCGCTAGGGACCAAGTCCTTGGTAGATATGGCTCCTCCCTGCTTCCCTCTTT3690.12050514187929239No Hit
CACGCTGGGAGGAGCAAATCCAGGCAGAGAGGGAAGTTACTGAGACCCAGAA3670.11985199747886262No Hit
TTGGTTGGGGAGTGTGCGTGCGCTTAATATCAGAGTTTCAAAGGACATGCAG3670.11985199747886262No Hit
GACAATGGGAACTGTAATTGCCTGGGAAAGTATTGGAGATGTGGATGGGGGT3650.11919885307843285No Hit
CACGCTGGGGCTATGTTTAGTCTCTCTGGTTTTTAAGCAGGCTTACCCCTCA3640.11887228087821795No Hit
TCGCAAGGGTATGACGGTTAAGAATGTCAGTATACAAGAAGGAATAGAAAAC3640.11887228087821795No Hit
TAGTCGGGTACTTTCCAGCAGTCAGTCCCTGGTTCCTAGATGTGTTCTAAGC3640.11887228087821795No Hit
ATGCTTGGGACATTTTAAAATATGCTTAAAATTCAGACCTTTTACATAAAAC3640.11887228087821795No Hit
CGTAGAGGGTACCTTGTCTCTTTTTCATGCTGAATAATATTCTGTGGTATGG3640.11887228087821795No Hit
AACATCGGGAACAGAAGCCTAGCATAACAATGAAATTAAGAAATAATTGTTA3620.11821913647778819No Hit
TTTAGTGGGGAGGAAGGATTAGAAGATATTGACGAAGAAGGGGATGAGGATG3620.11821913647778819No Hit
GTGGCGGGTGCTCAGTGGATCCACAGTGCCTTCAGGAACTGTGAAGTTAACT3610.1178925642775733No Hit
CATGCTGGGAAGTCATAATTTTTACCTAAAATTGTGGAATATGCATGTGAAT3600.11756599207735842No Hit
TGATTTGGGATCCTTGATAATAAATAAAATAATCTATGTTACCTATTGTGTT3580.11691284767692865No Hit
GCACTTGGGGAAGAGAAGCAATTTTGGGGAAGGGTTTGAATTGTTTTCTTTA3580.11691284767692865No Hit
AACGTCGGGGGAACAAAGAACATATAGTACCAACAACGAGATGGTAGACAAA3550.11593313107628399No Hit
TTACAAGGGTGAGACCAGCCTGACCAACATGGTGAAACCCCATCTCTACGAA3540.11560655887606912No Hit
AGCGGCGGGAAAGTGGTACAAGATTTTATTGATATATTTTCTGATTATGCTA3520.11495341447563935No Hit
TCGCGCGGGAGATTGGGCCTTAATTAGCATTGTACTCTAATCAAAGGACTCT3520.11495341447563935No Hit
GCCACTGGGCAGAAAGCACTTTGGTCTTAATTGGTGACTTGGGGGTTATTTG3510.11462684227542445No Hit
TAACGCGGGACTGGAATGGAGGAGAAGGGGCAGTCACTGGTGTTAATAAAAT3510.11462684227542445No Hit
CAATGGGGATGGCTGTGGTTTCAATGTTTACTCTACCTGTAGTGTATGTTAA3500.11430027007520958No Hit
GGTTCCGGGAGTAATGTGAAGCATCTTTTTGTGTGATTGTTGGCTTTTATAT3490.11397369787499469No Hit
TAAAGGGGCCAATCATATTGGGAAAGTGCTAGCTGTGCTCACCTTCATGGGG3470.11332055347456492No Hit
CACCCAGGGCAGCTTTTTCTCCGTCTCCCACAACATCGAGGAGGAAGAATTC3460.11299398127435005No Hit
TCTCTTGGGAGCAAGATTAATAGATTAAATGCTGTAAAGTAGATAAATTGAC3460.11299398127435005No Hit
CGCGGGGGAATGTAAAATCTGTGGTAAGAAAACAACTATTCATTATTCAGTA3430.11201426467370539No Hit
TCAATAGGGTGACTTAGGAGTTGTATTTCTCAATGTACCTGAAAATCTCAAA3430.11201426467370539No Hit
CATATAGGGACTTAGTCACGGGGATGGTGGAAGAGGGAGAAGAGGAAGGGTG3420.11168769247349052No Hit
GCACTAGGGAATGATATTTATTGAATGAACAGGAAACAGGGAAAGACATTGA3420.11168769247349052No Hit
CAACTTGGGGATCTAGCCAAACAAAAGAACACTATACAACAACTAAAATGAA3400.11103454807306072No Hit
CATTGCGGGGGAGGGGAGAGGAATAAAGTTTTATGGGACACCTCCTTACTTC3400.11103454807306072No Hit
AAGCCGTGGGTACCAAGCACACTGCCTACCTCAGGCCGTTTGAACTTGCTAT3390.11070797587284585No Hit
GCTGCCGGGGTATTCCGTCAAGTAAGATAATAACAGCTGACCTGCCAACAGC3390.11070797587284585No Hit
TTATCCGGGACCTAATGATTATTTCTATAACATTAAGCATGGTAATAAGTAG3390.11070797587284585No Hit
TTCTGCTCGGGGATACATAAATAAGAATATATTTGGTTCCAAGCTTTTGTCA3380.11038140367263095No Hit
GCGTAAGGGGGAAGCAGCATTGTACACCATAGTAAGTTCTTTGGAATTCATT3370.11005483147241608No Hit
CTCTCAGGGCACTTGCCACAGATGGAGCCCGTGACGGTGTGGAGGGTTTCCT3370.11005483147241608No Hit
CGCGCTGGGACATGCACAAGTTTTGCAGATTGCCAAGGAAGTTGCCCTCTGG3360.10972825927220119No Hit
GTTACAGGGCTCCCAAGTAGCTGGGACTACAGGCACGTGCCACCACGCCCAG3360.10972825927220119No Hit
ACTTCAGGGATGGCTTGATTTTCTAGTCTAAACCTCTGGTCTCACACTTCTC3350.10940168707198632No Hit
ACGCAGGGGAACTCTCAGGTGCACTTATATAAAATTCCTCGGTCTGGACCTA3350.10940168707198632No Hit
ATGGCTGGGACTATTCGCACATTTAAGCATCTTTGTTTTACATAAAATTAGA3350.10940168707198632No Hit
TTGCGTGGGACGCCAGGAATAGAATTCATCAAAATTTAATTAATTTAAATGA3350.10940168707198632No Hit
GTTGCGGGATATATGAAAGTTCAAACAGAAATTGCATTGTTATTACAGAGAA3350.10940168707198632No Hit
GACAATGGGATAGGAAATTGTCCTTTCAGTGGTTTCTAGGTATAACAAACAA3350.10940168707198632No Hit
CTGACAGGAGTAGGGAGGACTGTTGAATAAGCCAATAGAGTCTTAGAAACTA3340.10907511487177142No Hit
GTGATGGGGGGTGTATGGACTGGAAAAATGCATGCGAGATGTTCCTTTGCTT3330.10874854267155655No Hit
ACGACCAGGGTGCTTTTTTTATGCAAATAACCGATTAGGTCAGGGGTCGATC3330.10874854267155655No Hit
TCACACGGGACAGCAAGACTTGGGAAATGCAGTGAGCACCCGAGGAAAGGAA3320.10842197047134165No Hit
AGTAGTTGGGGAAGCCGGAATTCTTTTAGGCAATTTAAACTTTCTGAAATAG3300.10776882607091189No Hit
GAACAGGGGAGGCAAGAAGTAGAATGTCATCTTTCACTCTAATGCTTTTTAT3290.10744225387069702No Hit
TGTTTTGGGAATGATAGATAATGGAGATACAGAATGACTCATTGGGTTCATA3290.10744225387069702No Hit
TTCTATGGGCTAATTATCTTGTAAAATTTTCTTTTAAAAAAAGTATTAGGAG3280.10711568167048212No Hit
ACAGCAGGGAATGGCACAATTGACTTCCCTGAATTTCTGACAATGATGGCAA3270.10678910947026723No Hit
CCTAGTGGGAAGGCCTGTATGTAAATGTTTATAGTTGATTCATCCATAAGAG3260.10646253727005235No Hit
GTCTAGGGGAAGGGGGTTGTCCTTATTATGAATAGATTGTTACTTTTCCAAT3250.10613596506983747No Hit
GTGCACGGGTATGAATAATCTCAATTATATTCATCACACTTTTTTCATACCA3250.10613596506983747No Hit
GCAGGGCTATACAAAATTATTTTTATCTTCTGTTATAATTAAATACAATCAT3250.10613596506983747No Hit
GTACCTGGGTCTCATTTATTTTTACTTTATGGCATTAAAATACAATAAATCT3250.10613596506983747No Hit
TCATATGGGGATCTTAGGCCGGGGAGAGTGACCGCGGCTGCTCCAGCGCTGA3250.10613596506983747No Hit
CTCAATGGGACATGCTGGTCCTTCTGTTTGAAAGACAATTCCTCTTTGTCAT3240.10580939286962258No Hit
GATGTGGGGAGCATTAATAGAAACAGAGGTGCAACTAACATCTCTTGGTGAA3240.10580939286962258No Hit
AGTCCCGGGGATCTTGGAACGGGAAGATGTGAATGTAATCATCGATTTGCAT3240.10580939286962258No Hit
GATGCTGGGTCACCCAGTGTTCTTTGGATGTCCCTTCCCTAGGGGGAAAGGC3230.1054828206694077No Hit
CTGTAAGGGTAACCTAGCCAAGCCAATACATAAAATTAACCCTCTCAGCATG3220.10515624846919282No Hit
TGATCAGGGACAACTTTATATTAAAACACTAAATATTTTTAATGACAAGTAG3210.10482967626897792No Hit
GTGGATGGGATAAGCAAATAGAAATCTCATGCTATTTCTCATTATTATTTTC3190.10417653186854815No Hit
AAATTAGGGGCATTCATTTGATGAACTTCACTTTGTCATGATATATTATTTG3190.10417653186854815No Hit
CAGCAGGGGAATTAATAAACGCACAGCCCTATGTGAACAGACAGGAATTTCT3180.10384995966833327No Hit
GTCAAGGGGCATTGTTGAAAGAAATTAAAGAAGACCTAAATAAATGGAAAGA3180.10384995966833327No Hit
CTTTCTGGGTTGATAGAATAAATGAATGAATATATGAATACATCAATGAATA3170.10352338746811839No Hit
TACCAGGGTCCTGGCAGGGGCCCCCCGGTTTGCCTCCCTAGAGCCTGACCAG3170.10352338746811839No Hit
TTTTCCGGGGGATGTATTAGATTATTGATTTTTAACTTAAAAATGCATCATC3160.1031968152679035No Hit
CTTCACGGGGAAGGGTCGATTTTTTTGAGGTTTCTGTAAAATGAGATAAAAG3160.1031968152679035No Hit
CAACTTGGGGTATGTGTGTGTGTTCAGATAAATAAGACTTATGCATGGTTGG3150.10287024306768862No Hit
CGCCAGGGTACCAGACTTGTGTAGTGAGATAGGATATTACATGCCCTGGTCC3140.10254367086747374No Hit
TTGATAGGGATTAAAGCAAAACTTAAAAAAATGTTGACGTCTGTTAAATCTA3130.10221709866725884No Hit
TCAACTGGGTTGGAGTACAAATTAGACTTATAGTCCTCCTGGAACTTGAGTT3120.10189052646704397No Hit
TGTTTTGGGTCTGTGAAGAAACTTTGCTGAGTTAACCATAAATGTTCATTCA3110.10156395426682907No Hit
CCTGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC3110.10156395426682907No Hit
GTCCACGGGATAAATACTGTCTTCAACTTAAAGAAAGAAGATTTGAGGTGCA3110.10156395426682907No Hit
CTATTCGGGAAGGACATTCTAGCAATTTTAGAACATGCAAGGATTTCAGAAA3100.1012373820666142No Hit
ACAAATGGGGGGAGAAGCTTTACAACCATGACAGTGCAGCCAGATCTGAAAG3100.1012373820666142No Hit
TAGCAAGGGACCTTCTCTTTGTGTCCTCACTTGAAAGGGAGAAAGAGAGCTC3100.1012373820666142No Hit
CAAGCTGGGCTATTTTCACAGTTTATTAAACTGCCTAACACACATGATTTCT3090.1009108098663993No Hit
CAGCCTGGGATGTTATGAAAGTTATATGTATTCTTCAGAAATTAAATCTTAT3090.1009108098663993No Hit
AGATATGGGCACCAGGGGTTCGCTCTAGATCTTTCCGCTTCCTGCACAGAAA3090.1009108098663993No Hit
ATAACAGGGGAAATCAAAGATTGAGATCTTGAGGTCTACCAGAGAAAAATGA3090.1009108098663993No Hit
CAGGCTGGTCTTGAATTCCTGACCTCAAGTGATCCACTCTTCTCGACCTCCC3080.10058423766618443No Hit
TAGGAGGGGTCATACAACCATTTTCAAATGAACCTTAGAAAAGTCTTAACAT3080.10058423766618443No Hit
ATAGCTGGGCACTAACATTTTATCTAATTTCTGTTGCTTATTACATTTTATT3080.10058423766618443No Hit
TTATATGGGGGAGGCGCTAAAGCAAAAACGATTTCCACCAGCAACAGAAAAC3070.10025766546596954No Hit
ACTCAAGGGATCTGTGTCTGGGCAAAATGGAGATGTTGTTGGGCAGCATTCA3070.10025766546596954No Hit
AATGCAGGGGACCTACTCTGAGAGAATGCAGCATTCTCGGTCCTCTGACACC3070.10025766546596954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)