FastQCFastQC Report
Sat 31 Dec 2022
SRR5848328.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848328.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302096
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGCCGTCT15810.5233435729039776RNA PCR Primer, Index 32 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGC15180.5024892749324719No Hit
TGCTCCGGGGGGCCAGCTGCAGACACTAGAACAATATTGAATCAGTAACTTT4450.14730416821142947No Hit
CGCAATGGGGATTGAAACTGTGTCAATTTTGTAGAAAAAGATATTCTGGGGT4440.14697314760870717No Hit
GTAAAAGGGTAAATTGCAAACTCTGAACTTTTTACAAAGAAAAATGGAAAAA4400.1456490651978179No Hit
GTACTAGGGATCACTCCAAATTACCTCTTAAAGATTTTCTCATTTTTTTTTT4380.1449870239923733No Hit
ACAAATGGGCCAGCCATCCCAGTTTACTAGATAACGTATTTTTCTACCTGAT4380.1449870239923733No Hit
ATTAGCGGGATACAAAGGATGTACTATTATTAACCCCATTTTATAGATAAAG4200.13902865314337165No Hit
GCATGCGGGGTGGAAAGTTGCCATTTTTATGGGTTTATAGGATGAAAAAATT4190.13869763254064932No Hit
TTTAGTGGGGAGGAAGGATTAGAAGATATTGACGAAGAAGGGGATGAGGATG4010.13273926169164768No Hit
TGCTCTGGGATTGAAAAACTGAATCATTTAAAATCACAAAAGAGAAAATCAA4010.13273926169164768No Hit
GTCTCTGGGGACATTGTGGACATCGGATACCCAAGGAGACGAAGCTGAAGCA4000.13240824108892538No Hit
GCTTGTGGGAATGATTTCCTAGATCACTTTAACTTTTTTGTGTCATGGACTC3960.1310841586780361No Hit
GTATGTGGGCTTGGTCGTCTTTCAAGAAACAATGGCTCCATCATTCCTACAT3940.1304221174725915No Hit
ACGCCTGGGTGAAACTATAAGTCTTTGAGGAAAATTATAAGGTAAAATTTTC3910.12942905566442456No Hit
GTGCCAGGGAATGAAGCCCAGTTGAGTTGCAGAAAATTGACCACTTTTTCAT3830.12678089084264604No Hit
TGGAGTGGGGAGTCATCCAGTCCTGGGCACTGAGGTCTGTTAGATGAAAGGA3810.1261188496372014No Hit
CAGAGTGGGGAAGGTCCTTTCTGGAAACAGGATTACAAACATATGGACCTAT3790.12545680843175677No Hit
GACAATGGGAACTGTAATTGCCTGGGAAAGTATTGGAGATGTGGATGGGGGT3780.12512578782903447No Hit
TTATCCGGGACCTAATGATTATTTCTATAACATTAAGCATGGTAATAAGTAG3780.12512578782903447No Hit
AACATCGGGAACAGAAGCCTAGCATAACAATGAAATTAAGAAATAATTGTTA3770.12479476722631216No Hit
ACGTCCGGGATACATACCTGGCTCCAAAACAAGGCTGTATCTTCTGCCACTG3760.12446374662358985No Hit
TCGAGAGGGAACTTTCAACTCATAACAAATGAATACTACTATCCAGAATATG3760.12446374662358985No Hit
TCTGTAGGGGAAAGAGCAAAATAGTTTATCACTACTAGAAGCAAGAGAAGCA3750.12413272602086753No Hit
CCTAGTGGGAAGGCCTGTATGTAAATGTTTATAGTTGATTCATCCATAAGAG3750.12413272602086753No Hit
GTGGCGGGTGCTCAGTGGATCCACAGTGCCTTCAGGAACTGTGAAGTTAACT3730.12347068481542291No Hit
TCTGGCGGGATTTCTATCTTGCTCAAATATATTGGTTGGCATTCTTTTGTAA3690.12214660240453365No Hit
AACTTTGGGGATATATAATTATATATGTGGCATTCCTGCCAAAATGTTTAAC3690.12214660240453365No Hit
TAGTCGGGTACTTTCCAGCAGTCAGTCCCTGGTTCCTAGATGTGTTCTAAGC3680.12181558180181133No Hit
GGTTCCGGGAGTAATGTGAAGCATCTTTTTGTGTGATTGTTGGCTTTTATAT3670.12148456119908903No Hit
GTTGCGGGATATATGAAAGTTCAAACAGAAATTGCATTGTTATTACAGAGAA3670.12148456119908903No Hit
GCTCACGGGAATCTGGGAGAAGTACCATTTGATTTATTGCTATACATTTCAC3660.12115354059636671No Hit
CTGGCGGGCTGGAAAGATAATATTACTGGTTGCAAATGCAGTTAAACCTATT3660.12115354059636671No Hit
GCACTAGGGAATGATATTTATTGAATGAACAGGAAACAGGGAAAGACATTGA3650.12082251999364439No Hit
TGTCTCGGGACTAGAGTAGCAGGTGGTGTTGGAATTACCTGTTGGATTTTAG3640.12049149939092209No Hit
ATTGTTGGGAACAGGCAAAATATAAATTTCTTTGTTTCATGTATGACTCATC3630.12016047878819977No Hit
CAAATCGGGATAACGAGAGCAGCTTAATAGAGCAAGCTCCAATACCTCAGGA3620.11982945818547745No Hit
TCAATAGGGTGACTTAGGAGTTGTATTTCTCAATGTACCTGAAAATCTCAAA3600.11916741698003283No Hit
TATTATGGGATTGATAAATATTACAAAACTGAATGCAAATGTCCTTTGTGGT3590.11883639637731051No Hit
TTGCGTGGGACGCCAGGAATAGAATTCATCAAAATTTAATTAATTTAAATGA3580.11850537577458821No Hit
GGTTACGGGTGCCACCTGTGTCACAGTATGTAAACTTCAAGGATAGGAAATA3570.11817435517186589No Hit
AAGCCGTGGGTACCAAGCACACTGCCTACCTCAGGCCGTTTGAACTTGCTAT3560.11784333456914357No Hit
CACTAAGGGATTTATGGATGAGGAAGTTCAAAGCATACTCACGAAAATGACA3560.11784333456914357No Hit
TTACATGGGTCACTTTCAGCAGTCCGGGTAAAATCTGTGGATCAGGGTTAAA3550.11751231396642126No Hit
AACGTCGGGGGAACAAAGAACATATAGTACCAACAACGAGATGGTAGACAAA3550.11751231396642126No Hit
AGCTTTGGGTCAGCCCAAAATCTCCTTAAGCTGATAAGCAGCTTCAACAAAG3540.11718129336369895No Hit
TCGCAAGGGTATGACGGTTAAGAATGTCAGTATACAAGAAGGAATAGAAAAC3530.11685027276097663No Hit
TTAAAGGGAGATGTGGAAGATGTGAAATTTGTCATCAATTATGACTACCCTA3520.11651925215825432No Hit
ATAGGTGGGGAGGAGCTACGGCCGGAAGATGGCGGCGGCCGCAGAGTTGTCG3520.11651925215825432No Hit
CATATAGGGACTTAGTCACGGGGATGGTGGAAGAGGGAGAAGAGGAAGGGTG3510.11618823155553201No Hit
TGTTTTGGGAATGATAGATAATGGAGATACAGAATGACTCATTGGGTTCATA3510.11618823155553201No Hit
GTACCTGGGTCTCATTTATTTTTACTTTATGGCATTAAAATACAATAAATCT3500.1158572109528097No Hit
CACAGCGGGAATCCAAAAACACTCCATGTAATACATAAGTTTCCACTGGATC3500.1158572109528097No Hit
GCACTTGGGGAAGAGAAGCAATTTTGGGGAAGGGTTTGAATTGTTTTCTTTA3490.11552619035008738No Hit
ATAAGTGGGATAACAGCTTATAGAACTTGAACTACTAAATATGAAAATAAGT3480.11519516974736507No Hit
ATGCTTGGGACATTTTAAAATATGCTTAAAATTCAGACCTTTTACATAAAAC3480.11519516974736507No Hit
CGTAGAGGGTACCTTGTCTCTTTTTCATGCTGAATAATATTCTGTGGTATGG3480.11519516974736507No Hit
CAATACGGGTGGTGAATACTCTTTATAAAGGAAGTAAAGAATGATTGTTAAA3480.11519516974736507No Hit
TAAGAAGGGGTGAAATTATAATTTATGAGAACTGCATGGAGCTCCTGACAAT3440.11387108733647582No Hit
GTCTAGGGGAAGGGGGTTGTCCTTATTATGAATAGATTGTTACTTTTCCAAT3430.1135400667337535No Hit
TTTGGTGGGCTTCTTATACTTTTTAATGATTTTGTAGATAACCAGGCAAATC3420.1132090461310312No Hit
TTGGTTGGGGAGTGTGCGTGCGCTTAATATCAGAGTTTCAAAGGACATGCAG3420.1132090461310312No Hit
CTGTAAGGGTAACCTAGCCAAGCCAATACATAAAATTAACCCTCTCAGCATG3420.1132090461310312No Hit
TTCTGCTCGGGGATACATAAATAAGAATATATTTGGTTCCAAGCTTTTGTCA3410.11287802552830888No Hit
CACGCTGGGGCTATGTTTAGTCTCTCTGGTTTTTAAGCAGGCTTACCCCTCA3410.11287802552830888No Hit
AGACGGGGGGGCCGTGGCCCGGCTGGGTCGGCTGAAGAACTGCGGATGGAAG3410.11287802552830888No Hit
TAAGTCGGGGATTTCCACCAGCAACAGAAAACTTCCTTTATCATCTAGCAGC3390.11221598432286425No Hit
CCGTTTGGGATGAATATTGCATCTCTAATCTAATCTAAAAATGTTGTTTTGA3380.11188496372014194No Hit
GTCAAGGGGCATTGTTGAAAGAAATTAAAGAAGACCTAAATAAATGGAAAGA3380.11188496372014194No Hit
ATTTGCGGGGTATGGATTTCTTCTGGCAGATCTGCATGAGCTCTCAGTGATG3380.11188496372014194No Hit
TCAAATGGGGATGGAATATTGTGATTGACAGCCCCCACTTGCTTGCAATGAC3380.11188496372014194No Hit
GTGCTTGGGACACCTTTTACGATGACTACTTTTGGTGGAGTTGAAATGCTGT3370.11155394311741962No Hit
GATGCTGGGTCACCCAGTGTTCTTTGGATGTCCCTTCCCTAGGGGGAAAGGC3360.1112229225146973No Hit
GTTACAGGGCTCCCAAGTAGCTGGGACTACAGGCACGTGCCACCACGCCCAG3350.110891901911975No Hit
GCGTAAGGGGGAAGCAGCATTGTACACCATAGTAAGTTCTTTGGAATTCATT3350.110891901911975No Hit
TCAACTGGGTTGGAGTACAAATTAGACTTATAGTCCTCCTGGAACTTGAGTT3340.11056088130925268No Hit
AACCATGGGGAAGTAGAAAGAAATATAAATCATTCAAGCCAATAGCTGGATT3330.11022986070653036No Hit
AGCGGCGGGAAAGTGGTACAAGATTTTATTGATATATTTTCTGATTATGCTA3330.11022986070653036No Hit
GGTTTCGGGACAATCGAGTATTTTAGAAATTACATGAAACATGAAACAGTTT3320.10989884010380806No Hit
ATGGCTGGGACTATTCGCACATTTAAGCATCTTTGTTTTACATAAAATTAGA3310.10956781950108574No Hit
TCGCGCGGGAGATTGGGCCTTAATTAGCATTGTACTCTAATCAAAGGACTCT3310.10956781950108574No Hit
CGCGCTGGGACATGCACAAGTTTTGCAGATTGCCAAGGAAGTTGCCCTCTGG3300.10923679889836342No Hit
TGATTTGGGATCCTTGATAATAAATAAAATAATCTATGTTACCTATTGTGTT3270.10824373709019648No Hit
CTGACAGGAGTAGGGAGGACTGTTGAATAAGCCAATAGAGTCTTAGAAACTA3270.10824373709019648No Hit
ATTTGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA3260.10791271648747418No Hit
TCTCTAGGGAAGAAGGAAGTTTTTGAGTGGTTATCACATTAGAGAAATTTTT3240.10725067528202954No Hit
GCTGCCGGGGTATTCCGTCAAGTAAGATAATAACAGCTGACCTGCCAACAGC3230.10691965467930722No Hit
TTGATAGGGATTAAAGCAAAACTTAAAAAAATGTTGACGTCTGTTAAATCTA3220.10658863407658492No Hit
TTATATGGGGGAGGCGCTAAAGCAAAAACGATTTCCACCAGCAACAGAAAAC3210.1062576134738626No Hit
TAACGCGGGACTGGAATGGAGGAGAAGGGGCAGTCACTGGTGTTAATAAAAT3210.1062576134738626No Hit
AGCTAAGGGGAAGAATAATAATTCTACAAAAGTTCTGTACTTGAAAGAGATT3200.10592659287114028No Hit
CACGCTGGGAGGAGCAAATCCAGGCAGAGAGGGAAGTTACTGAGACCCAGAA3200.10592659287114028No Hit
CGCGGGGGAATGTAAAATCTGTGGTAAGAAAACAACTATTCATTATTCAGTA3190.10559557226841798No Hit
TGAATCGGGGGTGGGAACACGAAACAGGAGTCTCTTCTGTTCTGCAAGTTTG3180.10526455166569566No Hit
TATCACGGGCATTATATTTGAAGTGAGTTCCTTGTAGACAGCTTGTAGTTAG3180.10526455166569566No Hit
CAGATAGGGAAGTATGCAGACAACCAAATGAAGGCCTTTCAGCAGGCGAATC3170.10493353106297335No Hit
GTGATGGGGGGTGTATGGACTGGAAAAATGCATGCGAGATGTTCCTTTGCTT3170.10493353106297335No Hit
GATGTGGGGAGCATTAATAGAAACAGAGGTGCAACTAACATCTCTTGGTGAA3160.10460251046025104No Hit
TACCAGGGTCCTGGCAGGGGCCCCCCGGTTTGCCTCCCTAGAGCCTGACCAG3160.10460251046025104No Hit
GTGGATGGGATAAGCAAATAGAAATCTCATGCTATTTCTCATTATTATTTTC3150.10427148985752872No Hit
CAATGGGGATGGCTGTGGTTTCAATGTTTACTCTACCTGTAGTGTATGTTAA3140.1039404692548064No Hit
GCAGGGCTATACAAAATTATTTTTATCTTCTGTTATAATTAAATACAATCAT3130.1036094486520841No Hit
TTCTATGGGCTAATTATCTTGTAAAATTTTCTTTTAAAAAAAGTATTAGGAG3130.1036094486520841No Hit
TTTGCAGGGATACTTAATTTTATTTATGTATAGAATATTTGTATTTATTTTT3130.1036094486520841No Hit
GACAATGGGATAGGAAATTGTCCTTTCAGTGGTTTCTAGGTATAACAAACAA3130.1036094486520841No Hit
CAGCCTGGGATGTTATGAAAGTTATATGTATTCTTCAGAAATTAAATCTTAT3120.10327842804936178No Hit
CTTACAGGGGGATTAACCCTTTAAGTGCTGAATCTTTAAAATTTTAATATAT3120.10327842804936178No Hit
CTCAATGGGACATGCTGGTCCTTCTGTTTGAAAGACAATTCCTCTTTGTCAT3110.10294740744663947No Hit
TCTCTTGGGAGCAAGATTAATAGATTAAATGCTGTAAAGTAGATAAATTGAC3110.10294740744663947No Hit
TTCCCTGGAGGAAATTCAGAAGAAATTAGAAGCTGCAGAAGAAAGACGCAAG3080.10195434563847253No Hit
GGTACTGGGATGTCGTCACAAGGATATCTGGCAAAGGAAACCAAGCTGCTTC3080.10195434563847253No Hit
CTATCCGGGACCTGGATGCCTACGTGGGAAGGGACCTCGAATGTGGGACCCC3080.10195434563847253No Hit
AAATTAGGGGCATTCATTTGATGAACTTCACTTTGTCATGATATATTATTTG3070.10162332503575022No Hit
TTTTCCGGGGGATGTATTAGATTATTGATTTTTAACTTAAAAATGCATCATC3070.10162332503575022No Hit
AGTCCCGGGGATCTTGGAACGGGAAGATGTGAATGTAATCATCGATTTGCAT3070.10162332503575022No Hit
TTCGGTGGGGCTTGATAATAATAGCTCTTTGCCAGTAAGCTTTACTTTGTTG3040.10063026322758327No Hit
CAGCAGGGGAATTAATAAACGCACAGCCCTATGTGAACAGACAGGAATTTCT3040.10063026322758327No Hit
ATCTTAGGGGGGAAACCGTTTTCTTGTAAGCCCCTAGAGGCAGATCAGGTAA3040.10063026322758327No Hit
AAGTAGGGGAGGGAGAATTGAAGGGGTTAAAGTGTTAAATATGTTGCCTAGA3040.10063026322758327No Hit
CAACTTGGGGATCTAGCCAAACAAAAGAACACTATACAACAACTAAAATGAA3040.10063026322758327No Hit
GTGCACGGGTATGAATAATCTCAATTATATTCATCACACTTTTTTCATACCA3030.10029924262486097No Hit
GCAAGTGGGCCCAGTGGATGGGGTTCTTCTGTAAAACTGTTTGCACATGGCC3030.10029924262486097No Hit
TGTCCCCCCCCAGTTGATTCTGATGTACAGCCAGGTTTGGGAACTGGCCTTC3030.10029924262486097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)