FastQCFastQC Report
Sat 31 Dec 2022
SRR5848327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288063
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGCCGTCT14640.5082221597358911RNA PCR Primer, Index 32 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGC13440.46656460565917873No Hit
AAACTGGGCGATCTTGTGGCGGGGATGGTGAACTTGGGTCTGTCCCGGTTGG3890.13503990446534264No Hit
TAGCTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA3680.127749832501918No Hit
TAATGTGGGAAGGATCTGAGTATGCAGATAAGTTGGGGAAGGAGAAATGGCA3660.12705553993397278No Hit
GTAAAGGGGATGGATTGAAACAATAAATATTTTTGAAGTGCCATATTATTCA3650.12670839365000017No Hit
CGTAAAGGGATAGGGAAAAGTGAGCACAGTGCTATTGTCCACTTAGTCTTGG3520.12219549195835633No Hit
CGCAATGGGAAAGATGGCAAATGTTTTCAGTATATCCTGAAGGGATGTATCT3490.12115405310643852No Hit
GTCAAGTCTGTCCCCAGGGGAGGTCCCCCTTTCGGGAGGAAGTTTTTAAGGG3440.11941832168657551No Hit
ATAGCGGGGAGTGGGGAGAGTTTGTACATAATTGCTTAACTGGGTTTTTGGT3440.11941832168657551No Hit
GCTCGGGGGTCATTCTAAGAATTAAATATTGCCAAAACACATGAAAAGATTC3410.1183768828346577No Hit
GATCTGGGGAGGTGGCCAAGACTAAGCAGCAGATTGAGGAGCAGCGGGTGCA3320.11525256627890428No Hit
GTGGGTGGGGTGATTGAGAGAAGGACCTATAAGTTCGGGGTCGTTTTCTATA3290.11421112742698646No Hit
ATCAAATGGGGTTCTTTAGTTTTGCCAAAAGGTGTCTCAGATGATAAAGTTG3250.11282254229109605No Hit
GTCGCGGGGAACGGCGGGGAGTCCTTTGCTGTCGCACTCTGCCACCTCGCAG3220.11178110343917824No Hit
TGTAGAGGGATCTAAAAAGCACATTGCTTTCTTTGATAACTGGCTTAGAGAT3200.11108681087123302No Hit
AAATCAGGGGCCTAAAATTCCTGTGATGAAAGATGATATAGAGGATTCAGAC3170.11004537201931522No Hit
AAACATGGGATAAAAGAAATAGCCAAATAAAGAGATAATCTGTCTCTCCCAT3150.10935107945137No Hit
CTAGGCGGGGAAGGGCAACATTATTCTATAATTAAGAATTAAGAATGAATTC3120.10830964059945221No Hit
TAATGGGAGACTTTTGCAAGCTCAAAAAATGAAAATAATATCTGCTTAACCA3100.10761534803150699No Hit
AAGTGGGGGAGGCGTGATCTGGCCTTCGACTCGCTATGTCCACTAACAATAT3090.1072682017475344No Hit
GTCTACGGGCAGACTAAAAGTAAAAAAAATAAGCTTTATATAATTAGGGAGA3070.10657390917958919No Hit
ACTACCGGGTACCTTCTTAAACAGGAGTTTAGGAGCAGTGGGTCCCCATCTT3060.10622676289561658No Hit
TCTTCCGGACTTGCAAGCTCGGAGGCGAGCTGTGGGCTGTGGAAATACCTGT3040.10553247032767138No Hit
GCTACAGGGTAATTATTTGAGTAGATTAATAATGAAAATACTTTGGAAATAA3010.10449103147575356No Hit
CTGAGTCGGGGCAGAAGGAGGAGGACTTCAGGGCATTACTATGAAAGACAGT3010.10449103147575356No Hit
TAGGACGGGGGGCCAAATCTGGCCCACTAGCTGTTGTGTAAATAAAGTTTTA3000.10414388519178097No Hit
ACACGACGGGCAATTATATAATACATGACAAAACACAAATGAAATCCAATGT2990.10379673890780837No Hit
GATAACGGGGTAGTTCTAGTCAGGATGGGGAGTGAGCACTATTGAGAACAGG2970.10310244633986315No Hit
AAAAAAGGGGAAACTTTCTTTCTAAAGAAAGATACTCACATGAGTTCTTGAA2960.10275530005589054No Hit
GCATCTGGGCTGCGCCCAGCAGCAACTTGCTTTTTTTCTTTTGTTAAAGATA2960.10275530005589054No Hit
TGTTTAGGGAGCACATAATAGGGATTTGATAAGTGTTCATTACAGTTTAAAA2950.10240815377191795No Hit
TCAGTCGGGAGGCTGAGAAATAAGTTTGACAAGGTAATGAAAACTATCTAGA2940.10206100748794535No Hit
GCTACCGGGAGCTGTTCTGTAGGCTGCATAACCACTCTGATATTTAGGTATC2940.10206100748794535No Hit
GTATTTGGGACCACTGCTGCGGCCACTATGCCTGTGGTGCCCTCTGTGGCCA2930.10171386120397274No Hit
AGCAGCGGGGTTTGGGAATTAGAAAAATTTACATTCTCTCCCAGGTAACATA2920.10136671492000013No Hit
TATCTAGGGACGCTCGGGAGCGGCTGGGGGCGGTGTACGGGTTCGTCCGGGA2920.10136671492000013No Hit
TTTGGAGGGATTCTGGCAGGTGGGGGGACATTTGGGTGATGCTTGTTGTTAA2920.10136671492000013No Hit
GAGATGGGAATTACTTTTAATGTAGTTTTTCTTCATTATTGTCATTGCGTGT2910.10101956863602754No Hit
ACCTATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2910.10101956863602754No Hit
CGTGGCGGGGAAGCTAGGGTCGCCGCCACTGCCGCAGGAGGCGTGAGGGGAT2900.10067242235205494No Hit
ACCGTGGGGTACTTCATTGAAGATGCCACGGTTGTTATTGAAAAAGTGCGAA2900.10067242235205494No Hit
CATTGAGGGGACAGTTGTGGCATTTACTCACTTTAGCAGATAGCTAAAAGGG2890.10032527606808232No Hit
AGGCCGGGGGGTTTGGCAGCTCAACGAGGCTCATTGCGATATCTTCTTGAAT2890.10032527606808232No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)