FastQCFastQC Report
Sat 31 Dec 2022
SRR5848326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291262
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGCCGTCT14150.4858168933812169RNA PCR Primer, Index 32 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGC13570.46590355075499035No Hit
ATAGCGGGGAGTGGGGAGAGTTTGTACATAATTGCTTAACTGGGTTTTTGGT4230.14523006777403163No Hit
AAACTGGGCGATCTTGTGGCGGGGATGGTGAACTTGGGTCTGTCCCGGTTGG4070.13973673187714153No Hit
GTAAAGGGGATGGATTGAAACAATAAATATTTTTGAAGTGCCATATTATTCA4050.1390500648900303No Hit
GGTATCGGGGCTTCCGTCCTCCGCGCCTTCCGTCGGTCGGTCCTTGCTTCCT3600.12360005768002691No Hit
TAGCTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA3590.1232567241864713No Hit
GTGGGTGGGGTGATTGAGAGAAGGACCTATAAGTTCGGGGTCGTTTTCTATA3500.12016672274447061No Hit
TAATGGGAGACTTTTGCAAGCTCAAAAAATGAAAATAATATCTGCTTAACCA3450.11845005527669247No Hit
GTCGCGGGGAACGGCGGGGAGTCCTTTGCTGTCGCACTCTGCCACCTCGCAG3430.1177633882895812No Hit
GCTCTGGATTTAACCATTGTTCATTGTCAGGCTATATTTTTGTACAATCATT3430.1177633882895812No Hit
GATAGACGGGAATTTTCTTTTAAAAAGAACATAATGAAGTAACATTTTAATT3390.11639005431535868No Hit
AAGTGGGGGAGGCGTGATCTGGCCTTCGACTCGCTATGTCCACTAACAATAT3380.11604672082180306No Hit
GATCTGGGGAGGTGGCCAAGACTAAGCAGCAGATTGAGGAGCAGCGGGTGCA3370.11570338732824742No Hit
CTAGGCGGGGAAGGGCAACATTATTCTATAATTAAGAATTAAGAATGAATTC3360.11536005383469179No Hit
CGCAATGGGAAAGATGGCAAATGTTTTCAGTATATCCTGAAGGGATGTATCT3360.11536005383469179No Hit
TATCTAGGGACGCTCGGGAGCGGCTGGGGGCGGTGTACGGGTTCGTCCGGGA3340.11467338684758052No Hit
CGTAAAGGGATAGGGAAAAGTGAGCACAGTGCTATTGTCCACTTAGTCTTGG3310.11364338636691365No Hit
CTATGCGGGGCAGACAGTAGGTGTTAACGGCTGAGATCACTAAATGAATAGG3290.11295671937980238No Hit
TTGAGGGGTTAGTAATATTTCTCCAGAGAACCACATTTCTTTAACAAATGTA3280.11261338588624675No Hit
GTCAAGTCTGTCCCCAGGGGAGGTCCCCCTTTCGGGAGGAAGTTTTTAAGGG3260.11192671889913548No Hit
GGGAATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC3240.11124005191202421No Hit
TTAGCAGGGTAAGTAAAACAAATGTCATGCAGCTTGTTTGAACGAGTTCTAA3230.1108967184184686No Hit
TGTTTAGGGAGCACATAATAGGGATTTGATAAGTGTTCATTACAGTTTAAAA3220.11055338492491297No Hit
AGCAGCGGGGTTTGGGAATTAGAAAAATTTACATTCTCTCCCAGGTAACATA3210.11021005143135733No Hit
AAGAGGGGAACTTCCTCGAGATCGATGTGAGCAACCCGCAAACGGTGGGGGT3210.11021005143135733No Hit
AACCATGGGATAGTCTTAATGCAGGATCTCTGACAATGAGTGAAAAGCGGGA3190.10952338444424607No Hit
AAACATGGGATAAAAGAAATAGCCAAATAAAGAGATAATCTGTCTCTCCCAT3190.10952338444424607No Hit
GCTCGGGGGTCATTCTAAGAATTAAATATTGCCAAAACACATGAAAAGATTC3180.10918005095069046No Hit
AAATCAGGGGCCTAAAATTCCTGTGATGAAAGATGATATAGAGGATTCAGAC3170.1088367174571348No Hit
CTACATGGGGAAATAAAAAGTATCCACATTGAAAGGAAAAAGTAAACCTGTC3160.10849338396357919No Hit
ATCAAATGGGGTTCTTTAGTTTTGCCAAAAGGTGTCTCAGATGATAAAGTTG3160.10849338396357919No Hit
TCACAGGGGGCAGAAGAGAGAATAAGTGAGCTACCCCACGGTGGACATTCTG3150.10815005047002356No Hit
ACTACCGGGTACCTTCTTAAACAGGAGTTTAGGAGCAGTGGGTCCCCATCTT3140.10780671697646792No Hit
AGCTTGGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT3140.10780671697646792No Hit
TGTAGAGGGATCTAAAAAGCACATTGCTTTCTTTGATAACTGGCTTAGAGAT3120.10712004998935666No Hit
TAGGACGGGGGGCCAAATCTGGCCCACTAGCTGTTGTGTAAATAAAGTTTTA3120.10712004998935666No Hit
GTATTTGGGACCACTGCTGCGGCCACTATGCCTGTGGTGCCCTCTGTGGCCA3110.10677671649580102No Hit
TAATGTGGGAAGGATCTGAGTATGCAGATAAGTTGGGGAAGGAGAAATGGCA3080.10574671601513415No Hit
ACCTATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC3080.10574671601513415No Hit
TTACCAGGGGGGGCTAACCTTTATTAAACAGATCGCATATTATGATCTTGCT3080.10574671601513415No Hit
CATTGAGGGGACAGTTGTGGCATTTACTCACTTTAGCAGATAGCTAAAAGGG3060.10506004902802288No Hit
TCAGTCGGGAGGCTGAGAAATAAGTTTGACAAGGTAATGAAAACTATCTAGA3060.10506004902802288No Hit
CTGAGTCGGGGCAGAAGGAGGAGGACTTCAGGGCATTACTATGAAAGACAGT3060.10506004902802288No Hit
GTCTACGGGCAGACTAAAAGTAAAAAAAATAAGCTTTATATAATTAGGGAGA3050.10471671553446725No Hit
GATAACGGGGTAGTTCTAGTCAGGATGGGGAGTGAGCACTATTGAGAACAGG3050.10471671553446725No Hit
GTCACGGGCAGGAAATTCAGTCGCAGAGAAGAGGGACATTTCTCTCCCGCAG3040.10437338204091161No Hit
ACCGTGGGGTACTTCATTGAAGATGCCACGGTTGTTATTGAAAAAGTGCGAA3040.10437338204091161No Hit
GACAAGGGGAAGAAAGCAACAAATACATTGTTTGCAATATCATTTTGAGAGT3030.10403004854735598No Hit
CTTGGTGGGCACTCAAAATCACACAACTACATGAAAACCGAACAACCTGGTC3020.10368671505380037No Hit
CAGCCAGGGACCATGGACATTTTTTGTTAGACTTCTCCGGTACCTCCTTCTC3000.1030000480666891No Hit
TTGTCCGGGAAAGCATTTGGATTTGAAATTCAGGGAGTTTTTGATAGGGTAG3000.1030000480666891No Hit
GCTTTTGGGGCAGTATCTGGAAAATCTGGAGAAATTAATGTATAATGCATAC2990.10265671457313347No Hit
GCGATAGGGGAGAACAAATTTGCTGTGGGAAGTGGAGCACGACTCATTTCTG2990.10265671457313347No Hit
TGTGACGGGGGATCAACTAATCATGTCAGCAATGTAAATAACTTTATTTTTC2970.1019700475860222No Hit
AGCGTAGGGGGGCTATAAATTACAAACAAAACACATCCCAGACATTAGGAGT2970.1019700475860222No Hit
CTATTTGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC2970.1019700475860222No Hit
CGTGGCGGGGAAGCTAGGGTCGCCGCCACTGCCGCAGGAGGCGTGAGGGGAT2960.10162671409246658No Hit
GAATGGGGGACGCTCTTCCGATCTAAGCGGGGCAAGGCAGCGGTGCTTAATC2960.10162671409246658No Hit
TTATCTGGGAAAATCCAAATGCAGGATAGTAATTTTTGTTTACTTTTTTAAC2960.10162671409246658No Hit
ACGCAAGGGATTAGACAAAAGAAGATATAAAATTACAAAAGAAATTATTTAG2950.10128338059891095No Hit
AAAAAAGGGGAAACTTTCTTTCTAAAGAAAGATACTCACATGAGTTCTTGAA2950.10128338059891095No Hit
GTCATAGGGCTCACATGGTTTTTGGTAGGATTCAGTTTCTTGCAGACTATTT2940.10094004710535531No Hit
TGCTTGGGGAATGGCAAAGCTCTGCTGAACCAACCAACATAAGGAATTGCAC2930.10059671361179968No Hit
ACAAATGGGAAGGAGAAAAGTATGAAAACATTGGAGAAACCTGACAAACACT2930.10059671361179968No Hit
CCTCTTGGGAGGCCAAGGTGGAGTCTGAAGAGCCACAGGACAATGACTTCCT2920.10025338011824406No Hit
GGCAAGCGGGATGCCTTCTAACTTCATAAGCAAACCTTTAACTAATTATGTA2920.10025338011824406No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)