FastQCFastQC Report
Sat 31 Dec 2022
SRR5848325.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848325.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288078
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGC13830.4800783121237998No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGCCGTCT13510.4689702094571609RNA PCR Primer, Index 32 (95% over 21bp)
CGTAAAGGGATAGGGAAAAGTGAGCACAGTGCTATTGTCCACTTAGTCTTGG3670.12739605245801483No Hit
AAACTGGGCGATCTTGTGGCGGGGATGGTGAACTTGGGTCTGTCCCGGTTGG3660.12704892424968237No Hit
GCTCGGGGGTCATTCTAAGAATTAAATATTGCCAAAACACATGAAAAGATTC3600.12496615499968759No Hit
ATAGCGGGGAGTGGGGAGAGTTTGTACATAATTGCTTAACTGGGTTTTTGGT3590.12461902679135513No Hit
TAATGGGAGACTTTTGCAAGCTCAAAAAATGAAAATAATATCTGCTTAACCA3510.12184200112469538No Hit
TTATCTGGGAAAATCCAAATGCAGGATAGTAATTTTTGTTTACTTTTTTAAC3450.1197592318747006No Hit
TAATGTGGGAAGGATCTGAGTATGCAGATAAGTTGGGGAAGGAGAAATGGCA3430.11906497545803568No Hit
ATCAAATGGGGTTCTTTAGTTTTGCCAAAAGGTGTCTCAGATGATAAAGTTG3400.11802359083303826No Hit
GTGGGTGGGGTGATTGAGAGAAGGACCTATAAGTTCGGGGTCGTTTTCTATA3340.11594082158304349No Hit
GATCTGGGGAGGTGGCCAAGACTAAGCAGCAGATTGAGGAGCAGCGGGTGCA3300.11455230874971362No Hit
GATAGACGGGAATTTTCTTTTAAAAAGAACATAATGAAGTAACATTTTAATT3290.11420518054138114No Hit
CTATGCGGGGCAGACAGTAGGTGTTAACGGCTGAGATCACTAAATGAATAGG3260.11316379591638376No Hit
GTAAAGGGGATGGATTGAAACAATAAATATTTTTGAAGTGCCATATTATTCA3250.11281666770805128No Hit
AAACATGGGATAAAAGAAATAGCCAAATAAAGAGATAATCTGTCTCTCCCAT3210.11142815487472144No Hit
CTAGGCGGGGAAGGGCAACATTATTCTATAATTAAGAATTAAGAATGAATTC3190.11073389845805649No Hit
TGTAGAGGGATCTAAAAAGCACATTGCTTTCTTTGATAACTGGCTTAGAGAT3180.11038677024972404No Hit
CTTCAAGGGGGGAAATCTCAGGCTAAGAACAACAAGAAGCCTCCCCCTGCGA3160.1096925138330591No Hit
TCACAGGGGGCAGAAGAGAGAATAAGTGAGCTACCCCACGGTGGACATTCTG3120.10830400099972924No Hit
AAGTGGGGGAGGCGTGATCTGGCCTTCGACTCGCTATGTCCACTAACAATAT3080.10691548816639938No Hit
GCCTTGGGGCAATTTGATTACCTCACCTACTTATCTTTCTGTGGTGAGAATG3060.10622123174973445No Hit
CGCAATGGGAAAGATGGCAAATGTTTTCAGTATATCCTGAAGGGATGTATCT3040.10552697533306951No Hit
TAGGACGGGGGGCCAAATCTGGCCCACTAGCTGTTGTGTAAATAAAGTTTTA3040.10552697533306951No Hit
GGTATCGGGGCTTCCGTCCTCCGCGCCTTCCGTCGGTCGGTCCTTGCTTCCT3030.10517984712473706No Hit
GTCTACGGGCAGACTAAAAGTAAAAAAAATAAGCTTTATATAATTAGGGAGA3000.10413846249973965No Hit
ACATCTGGGATTGAGAAAATGCTCACAGACCGGCGGTCTACAGACCTTAATG2990.10379133429140719No Hit
GCTTTTGGGAAGGAAGGTTCGGGTGGTATTTGCAGATGTGTTGACGTTTTGC2980.10344420608307471No Hit
TTACCAGGGGGGGCTAACCTTTATTAAACAGATCGCATATTATGATCTTGCT2980.10344420608307471No Hit
TAGTGCGGGACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATA2980.10344420608307471No Hit
GCTCTGGATTTAACCATTGTTCATTGTCAGGCTATATTTTTGTACAATCATT2970.10309707787474226No Hit
GATAACGGGGTAGTTCTAGTCAGGATGGGGAGTGAGCACTATTGAGAACAGG2970.10309707787474226No Hit
GTCACGGGCAGGAAATTCAGTCGCAGAGAAGAGGGACATTTCTCTCCCGCAG2960.1027499496664098No Hit
AAAAAAGGGGAAACTTTCTTTCTAAAGAAAGATACTCACATGAGTTCTTGAA2960.1027499496664098No Hit
AAATCAGGGGCCTAAAATTCCTGTGATGAAAGATGATATAGAGGATTCAGAC2950.10240282145807733No Hit
TCACCGGGAGAGAAAAAGACAAAAAACGGGACCGAGAAGAAGATGAAGAAGA2940.10205569324974485No Hit
ACCGTGGGGTACTTCATTGAAGATGCCACGGTTGTTATTGAAAAAGTGCGAA2940.10205569324974485No Hit
TCAGTCGGGAGGCTGAGAAATAAGTTTGACAAGGTAATGAAAACTATCTAGA2920.10136143683307994No Hit
CAAAATGGGGATTAACAATACCACTGTCTTCTGCCTCCAAATTCTGTCCCAA2910.10101430862474747No Hit
GCTACAGGGTAATTATTTGAGTAGATTAATAATGAAAATACTTTGGAAATAA2910.10101430862474747No Hit
GTCTCAGGGGCTCCACCACAACTGGCTAACTTTTTTATTTTTGTGTGTGGAG2910.10101430862474747No Hit
ACCTATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2900.100667180416415No Hit
GCTACCGGGAGCTGTTCTGTAGGCTGCATAACCACTCTGATATTTAGGTATC2900.100667180416415No Hit
GCACACAGGGCCTTACGGATTTTTTCCGGAACCATAATTTGGAGTTGTCGAA2900.100667180416415No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)