FastQCFastQC Report
Sat 31 Dec 2022
SRR5848279.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848279.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332655
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGC19060.5729659857810644No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGCCGTCT18330.5510213284033008RNA PCR Primer, Index 32 (95% over 21bp)
TAGGCTGGGAGGATACAAAGTGTGATTTGTTGGAAACATGTCCCAAATTTCT4740.14248996708301392No Hit
TAAGGCAGGGCAGGACTCTTGCAGGAGAAGCAATGGAGTCAGTGTGGTGTGG4550.13677834392989732No Hit
TTCTTAGGGCGATAGAATAAGGAAATTGAGCCAAAATCTGCAACTACCTGGC4460.13407283822578947No Hit
TAAGCAGGGTGTGAAACTTACCCTAGATTTGTAAAGATAATTGTTTCAAACT4440.13347161473598773No Hit
AAAAATGGGACTGTGGTGGGGCTTGAGAATTTGCACGTAATTAATTTCCAGA4360.13106672077678075No Hit
TTCTAGGGGAAATTATCTGCAGTTAGTTTCTTTGAAGAACATAGTTGGAAGT4300.1292630503073755No Hit
GGTCTTGGGAAGACATCCAGTTAAAATAGGAAAAAATTAACATCTAGAATCT4250.12775999158287113No Hit
CAAGCCGGGTGCTTGCAAAAGTAAACAGTGAAATAATTAATGTTGGCAAATA4240.12745937983797026No Hit
ATGTAAGGGCAAAAAATTGAAGTATCTAAGAATGCATCTAAACAAGGAGGTG4190.1259563211134659No Hit
AACAAAGGGTCTTGCCAACCAACCATGAGCTCCCAGATTCGTCAGAATTATT4160.1250544858787633No Hit
ATAATAGCGGTACTAGGCAGAATTCACTAATGGAGTAGATATGTGGCATTCA4160.1250544858787633No Hit
TGTGTGGGGGGGCTAACCTTTATTAAACAGATCGCATATTATGATCTTGCTG4120.1238520388991598No Hit
CTGTTTGGGGCACAATGGTAAGATGATCCATACAGGTTTTATACATATTTCT4120.1238520388991598No Hit
ACATTTGGGATGACAATCTGTTTTTAAATTGGATTCTATGAAAATGCATAAT4110.12355142715425893No Hit
TTTAAGGGGGTCATCATTTAAGGATGAGAGAGGATGGGGAAAGGCAATGTAG4110.12355142715425893No Hit
CAGCCTCGGGATTGGCTTTCTTTTTAGTCTTTGCCACTTTGTAAAAGGAAAA4090.12295020366445716No Hit
AGATCAGGGTCTTTCCAAGTATTTGGTAGTTTTGTCAAGACTCGATACACAG4090.12295020366445716No Hit
CCCATTGTGAATGTTGTCCTGTGTCATCTGTCAGCTTCCCAACAATACTTTG4080.1226495919195563No Hit
GGCGCTGGGACATCAATCCAGACTATTAAAAATTTTATGTGAGGAAATGGGT4060.12204836842975456No Hit
GTAAGAGGGATGGTTTCTATTTGTTTTGAAAAGCAGCTACAACCTTCAAATG3950.11874163923584495No Hit
TCATCGGGGGGAAGAAGATGCCTAATATGTTAGTTTTTCACCCTTGGATCCT3950.11874163923584495No Hit
TATTCGCGGGACTGCAGAAGAATAACCTGACCTTTTCTTTGCATTTGTTTTG3930.11814041574604318No Hit
TTTCGTCGGGGGAGAAAAAGCAGAAGAGGCAGAAGAACTTCGATTAGATCTC3920.11783980400114231No Hit
AGATCTGGGTAAAAGTGAATATTGAAGCTTCTTAAAGTTGGTCTTAATTTTT3920.11783980400114231No Hit
GACAATGGGAGGATATGAAACTGGGAGCAGGAGTTAGAATCTGTGATAGGAC3890.1169379687664397No Hit
TTCCGTGGGGCCTAAATAAATGAGCTGTTGGAAGAAAAGAATCACAGTACTT3840.11543491004193535No Hit
CGTGACGGGGAAAAACAAATGCAAACAAATTACAAATTTCAAATGATAGAAG3830.11513429829703448No Hit
CGTTATGGGATAAAGTGCTAGAAAATCATGTTCCTTGTCCTGAGTAAGAGTT3810.11453307480723271No Hit
TTTCTAGGGAGATGTTGTAACTTTTCACTGTACAAGCATTGCAATGATTGTG3810.11453307480723271No Hit
CAAGCTGGGATTGAGCTCATCCATAAAACAAAGAAGCTATTGCAGGCTGCAG3810.11453307480723271No Hit
TCGCATGGGGAATGAACAAAATACTATAGAAAAAGAAGAGTTCATGTTGAAA3800.11423246306233184No Hit
ACAATCGGGACCAAAACAGACACAAGAAAAAATCATGTAGTCTGAATATTCA3800.11423246306233184No Hit
ACATATGGGAGAGTGACTAAAATAATAAATAGGAATAACATTAAATGCTGGT3780.1136312395725301No Hit
CCTCTAGGGATTTCCAAGAATTCTCCCAATAATATGAGCCTGAGTAATCAAC3770.11333062782762923No Hit
TGCCAACGGGCACGACGCTCTTCCTTTTCTAGAAGCGGGGGTAATAAACTGA3760.11303001608272835No Hit
GCTTATGGGAAACAGAAGATTAGACAAATTCCTTACTCTTAAGTATTTTTTT3730.11212818084802574No Hit
TTATGCGGGAAGATAGACAATTGATGCATAAGGAAATGTAAGTATATTTACT3690.11092573386842224No Hit
ACGCAAGGGAGCTGGTAGGTAGACAGCAAGAATCCAGGTCTTCTGACTCCTC3690.11092573386842224No Hit
TAAATAGGGGTAGTTAGCTCATTCGTACAGACATTTTCAGTATATGCAGAAA3680.11062512212352137No Hit
CAAAGCGGGAAGCTTTTTTTCTTTTATAAGTTTGTGCTTTCTATTTCTTATC3670.1103245103786205No Hit
ACGCCAGGGAGGTTTACTCACGTCATCCAGCAGAGAATGGAAAGTCAAATTT3650.10972328688881874No Hit
CCCCAGACTTTAGCCAAAATTTCCCCACGAACAGAGATTCTTAAATATCTCC3630.109122063399017No Hit
AAACTGGGGTATCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAG3630.109122063399017No Hit
TACAGAGGGATTTTTGTATGTGTTAAAAAGACTACTATATCACAATTAAAAG3620.10882145165411614No Hit
GACCTGCAGTTTGAAAAACACTGTAGTTAGTTTTATCTCCACCTCAGGCCAG3580.10761900467451263No Hit
GTCGCCGGGCATACTGATTTGAAGCCATATGAATGTAAGCAATGTGGGAAAG3570.10731839292961176No Hit
AGGCAGGGAAGATGGGCTTTGGAAGAGGCTTGCGATTTGGGTAATATTGAAA3560.10701778118471089No Hit
ATTCCCGGGTGGGAAAAGCGATTTTTTTCATGTATTCATTCTGAATAGTTGA3560.10701778118471089No Hit
GCAGCGGGGGCACAAAAGTCTCAATGTTCCATTCAGTCTGCAGCTATTCACA3550.10671716943981002No Hit
TTGAAAGGGGCCAGGTATATGGTCAATTTTGAAAACTGTTACACAGATGACA3550.10671716943981002No Hit
CAGGCGGGGTTGGCATAGACATAAAGGATAAAAGGAAAATAACCGTCTGCCG3550.10671716943981002No Hit
TTAATGGGGATACTCATCCACATGAATGTCAGAGTTGCATGGGCCACTTCTT3540.10641655769490913No Hit
AATCCAGGGTACTAATTTCTTAGAAATGCATCACTCTTTTTTCCTTATTTGC3540.10641655769490913No Hit
GACTAAGGGATAATTCCTATAGTTACAATAGTATATTCCCATAATTGTCAAT3540.10641655769490913No Hit
ATTTCCGGGACACATAACATTTTTTAGTTCATTCTGCCTTTCCTATTTGTTC3520.10581533420510739No Hit
TAACAAGGGGAGCTTTTCTGGATTTTTATGATCTGAATGGCATATTTTCAAA3510.10551472246020652No Hit
GTAGTCGGGATTTTTCAACATGTGACATCAGAATAAAGTTCTGTATTTTGGT3500.10521411071530565No Hit
TGCTATGGGAACAGTTCTAATGGTAACCATATATTTTGGCCCATGGGCCATT3500.10521411071530565No Hit
TCATTTGGGAGTTTCCAAAAAACAAATCTTTATATATACCTTTCCAGTGCCA3490.10491349897040478No Hit
GTACTGCCGGGAAGATTTTGCAGATTTATCAATTTGGGTTTTTGTACTGAAG3490.10491349897040478No Hit
AGTACCGGGATTCTTTCTATGATGTTACTTCTTTCTTTATTTAGATATGGGT3490.10491349897040478No Hit
TGCCATGGGGCAAGACAAAGGAGGAGGAGGAGAAAGAAAAGAAGAAACAAAA3490.10491349897040478No Hit
GTTACTGGGAGCTGTTGTTGTTGGACTTGTGTTCCAATGAGTGACTAGGAAA3490.10491349897040478No Hit
AGCATTGGGAGTGCTATAAATTTCCCTCTTAACAGTGCTTTAGCTGTGTCCC3470.10431227548060303No Hit
AATTCGGGGGGTTACTGTTTTCATGAAGAAGAAATAGTTACTGCAAATGCTT3470.10431227548060303No Hit
ATACCAGGGCATTTGGTATGAACCAAAGTGCATTCCTTTTATATGTGCCTGG3460.10401166373570216No Hit
ACTTGCGGGTGGGTGTGAAAGTGGTGGATCCAGCAGGAAGCCCAGCCTCACA3450.10371105199080129No Hit
GGTGCAGGGGTAGGATATTATTAAATTTTTTATTGCTAAATATGTTTCTTTC3450.10371105199080129No Hit
TGCTGCGGTCCAGCAGAATTGAAAACTCTGGCAATAAAACATGGACTCAACC3450.10371105199080129No Hit
TTACTGGGGCTATGAAGCTTATTGGGACATAGTACTCATACTATAAATTTCT3450.10371105199080129No Hit
AGCAATGGGAACACACACACACACAAAACAACAGCAACAACAACAGAACAAC3440.10341044024590042No Hit
GCTGACGGGCAACTTGGATTGTTTAAACTTTCATTCACTAGATATTCATGAA3440.10341044024590042No Hit
GGCGGCGGGTATATCAAAGTCAGTTGAATGAATATGACAATAGACAACAATT3420.10280921675609865No Hit
AACACCGGGAGATGATAGAAATTGACAGGGTTGCATTTGACATAAAATATCT3420.10280921675609865No Hit
GGTTCAGGGGACGCTCTTCCGATCTAGCTTCGGGACATATATGAAAGAAGGC3420.10280921675609865No Hit
TAGCCTGGGCCCTTTGGGAAACCAGAGCATTGGCTAAACTGGAACCTGAAAA3410.10250860501119778No Hit
ATGCGTGGGCATAGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAA3410.10250860501119778No Hit
CTTACAGGGATCTCTTCCAAAAGTCAACTTTTTGTTTCATTAATTCTTTGCA3400.10220799326629691No Hit
GTTCAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCC3390.10190738152139604No Hit
CAACAAGGGACCTGACACTAGGGGCTCTAGAGAGGCAGAGAGAGCACTCACA3380.10160676977649517No Hit
TCGCCAGGTGACAAGATGTTGTGAGGGGTTTTGAGTCATCCAGTCCTGGGCA3370.10130615803159429No Hit
TTCGATGGGGTGCAGTGGTGTGATCTCAGCTCACTGCAATTTCTGCCTCTGG3370.10130615803159429No Hit
AGCAGCGGGATGATATGATAGTCTCTTGAATAACTAAGTTAAAGTTGGTTAT3360.10100554628669342No Hit
GCATCGGGACTGTTTGTAACTAAGGTGTTCCTCACAATCCAAGCCAGAACTG3360.10100554628669342No Hit
GTAATTGGGCTGTACTTATTGTAGATTCAATGACGCAGTTAAGTCATCACCC3360.10100554628669342No Hit
GCGCTAGGGAATCCCCTCAAATTTTGCTAAAAGGATATGGTAAATATAATCT3360.10100554628669342No Hit
TTGCTAGGGGAAATGATACCTATGGAGTGTGATTCATTTTGCAGTGACCAAA3350.10070493454179255No Hit
TTTACAGGGACTCATGACAAATCAACTGTGACCCTCGCTCCTTCCATTTCTG3340.10040432279689168No Hit
ATACCTGGATAATCACATATTTGGAATGTACACGATGAGCCAAGCATGTTGC3330.10010371105199081No Hit
TTTCAAGGGGTTTTTGTGACTGTTTTTGTAAATTATTCAAAGTTGTTGGAAT3330.10010371105199081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)