FastQCFastQC Report
Sat 31 Dec 2022
SRR5848278.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848278.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331677
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGC19210.5791779351598091No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGCCGTCT18520.5583745632045635RNA PCR Primer, Index 32 (95% over 21bp)
TAAGCAGGGTGTGAAACTTACCCTAGATTTGTAAAGATAATTGTTTCAAACT5250.15828652574643404No Hit
TAGGCTGGGAGGATACAAAGTGTGATTTGTTGGAAACATGTCCCAAATTTCT4830.1456236036867193No Hit
TTCTTAGGGCGATAGAATAAGGAAATTGAGCCAAAATCTGCAACTACCTGGC4750.14321161853248793No Hit
ATGTAAGGGCAAAAAATTGAAGTATCTAAGAATGCATCTAAACAAGGAGGTG4550.13718165564690948No Hit
GGTCTTGGGAAGACATCCAGTTAAAATAGGAAAAAATTAACATCTAGAATCT4480.13507116863695703No Hit
TTCTAGGGGAAATTATCTGCAGTTAGTTTCTTTGAAGAACATAGTTGGAAGT4450.1341666742041203No Hit
AAAAATGGGACTGTGGTGGGGCTTGAGAATTTGCACGTAATTAATTTCCAGA4330.13054869647277323No Hit
TAAGGCAGGGCAGGACTCTTGCAGGAGAAGCAATGGAGTCAGTGTGGTGTGG4310.12994570018421536No Hit
AGATCTGGGTAAAAGTGAATATTGAAGCTTCTTAAAGTTGGTCTTAATTTTT4300.12964420203993643No Hit
TGTGTGGGGGGGCTAACCTTTATTAAACAGATCGCATATTATGATCTTGCTG4280.1290412057513786No Hit
TCATCGGGGGGAAGAAGATGCCTAATATGTTAGTTTTTCACCCTTGGATCCT4270.12873970760709968No Hit
AGCATTGGGAGTGCTATAAATTTCCCTCTTAACAGTGCTTTAGCTGTGTCCC4210.12693071874142614No Hit
GACAATGGGAGGATATGAAACTGGGAGCAGGAGTTAGAATCTGTGATAGGAC4210.12693071874142614No Hit
CAAGCCGGGTGCTTGCAAAAGTAAACAGTGAAATAATTAATGTTGGCAAATA4120.12421723544291585No Hit
ACATTTGGGATGACAATCTGTTTTTAAATTGGATTCTATGAAAATGCATAAT4100.12361423915435801No Hit
ATAATAGCGGTACTAGGCAGAATTCACTAATGGAGTAGATATGTGGCATTCA4100.12361423915435801No Hit
GGCGCTGGGACATCAATCCAGACTATTAAAAATTTTATGTGAGGAAATGGGT4070.12270974472152124No Hit
TTTCGTCGGGGGAGAAAAAGCAGAAGAGGCAGAAGAACTTCGATTAGATCTC4060.12240824657724232No Hit
AGTACCGGGATTCTTTCTATGATGTTACTTCTTTCTTTATTTAGATATGGGT4030.12150375214440555No Hit
GTAAGAGGGATGGTTTCTATTTGTTTTGAAAAGCAGCTACAACCTTCAAATG4020.12120225400012663No Hit
CCCCAGACTTTAGCCAAAATTTCCCCACGAACAGAGATTCTTAAATATCTCC4020.12120225400012663No Hit
CGTGACGGGGAAAAACAAATGCAAACAAATTACAAATTTCAAATGATAGAAG4010.1209007558558477No Hit
TTGTGCTGGGATGTAATAAAATGTTAGGTAGGACCTAACATTATATTATGCT4000.12059925771156878No Hit
TTTAAGGGGGTCATCATTTAAGGATGAGAGAGGATGGGGAAAGGCAATGTAG3980.11999626142301094No Hit
AACAAAGGGTCTTGCCAACCAACCATGAGCTCCCAGATTCGTCAGAATTATT3960.1193932651344531No Hit
GGTTCAGGGGACGCTCTTCCGATCTAGCTTCGGGACATATATGAAAGAAGGC3950.11909176699017418No Hit
CTGTTTGGGGCACAATGGTAAGATGATCCATACAGGTTTTATACATATTTCT3950.11909176699017418No Hit
TATTCGCGGGACTGCAGAAGAATAACCTGACCTTTTCTTTGCATTTGTTTTG3940.11879026884589526No Hit
TTCGATGGGGTGCAGTGGTGTGATCTCAGCTCACTGCAATTTCTGCCTCTGG3930.11848877070161634No Hit
ACAATCGGGACCAAAACAGACACAAGAAAAAATCATGTAGTCTGAATATTCA3930.11848877070161634No Hit
TGTTGCTGGGATGGGCAACATGTTCAGCTTCGCGGCGGTCATGGGTGCCCTG3920.1181872725573374No Hit
GACCTGCAGTTTGAAAAACACTGTAGTTAGTTTTATCTCCACCTCAGGCCAG3910.11788577441305848No Hit
CAGCCTCGGGATTGGCTTTCTTTTTAGTCTTTGCCACTTTGTAAAAGGAAAA3900.11758427626877956No Hit
GAAGCAGGGATTTCAAGAGAGTGGCAGGTCTGTTCCTGGTAAAATTTTAACC3900.11758427626877956No Hit
TTATGCGGGAAGATAGACAATTGATGCATAAGGAAATGTAAGTATATTTACT3900.11758427626877956No Hit
GCTTATGGGAAACAGAAGATTAGACAAATTCCTTACTCTTAAGTATTTTTTT3890.11728277812450064No Hit
CAAGCTGGGATTGAGCTCATCCATAAAACAAAGAAGCTATTGCAGGCTGCAG3890.11728277812450064No Hit
CCTCTAGGGATTTCCAAGAATTCTCCCAATAATATGAGCCTGAGTAATCAAC3870.1166797818359428No Hit
AGATCAGGGTCTTTCCAAGTATTTGGTAGTTTTGTCAAGACTCGATACACAG3850.11607678554738496No Hit
TTCCGTGGGGCCTAAATAAATGAGCTGTTGGAAGAAAAGAATCACAGTACTT3810.11487079297026928No Hit
CAGGCGGGGTTGGCATAGACATAAAGGATAAAAGGAAAATAACCGTCTGCCG3790.11426779668171141No Hit
TCGCATGGGGAATGAACAAAATACTATAGAAAAAGAAGAGTTCATGTTGAAA3780.1139662985374325No Hit
AATGCGGGAATAAATAAGATTGTTTCTTCCCTACTGAATAGATAAGTGTTTT3780.1139662985374325No Hit
ATTTCCGGGACACATAACATTTTTTAGTTCATTCTGCCTTTCCTATTTGTTC3780.1139662985374325No Hit
GGTGCAGGGGTAGGATATTATTAAATTTTTTATTGCTAAATATGTTTCTTTC3770.11366480039315358No Hit
TTCGGTGGGATGGCTTAGAAGTGATCTATATGATTGAGCCCATTGATGAGTA3750.11306180410459574No Hit
ATGCGTGGGCATAGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAA3720.11215730967175898No Hit
GCTGACGGGCAACTTGGATTGTTTAAACTTTCATTCACTAGATATTCATGAA3710.11185581152748006No Hit
ACGCAAGGGAGCTGGTAGGTAGACAGCAAGAATCCAGGTCTTCTGACTCCTC3700.11155431338320113No Hit
GCAGCGGGGGCACAAAAGTCTCAATGTTCCATTCAGTCTGCAGCTATTCACA3690.1112528152389222No Hit
ATTCCCGGGTGGGAAAAGCGATTTTTTTCATGTATTCATTCTGAATAGTTGA3690.1112528152389222No Hit
TGCCAACGGGCACGACGCTCTTCCTTTTCTAGAAGCGGGGGTAATAAACTGA3690.1112528152389222No Hit
ATACCAGGGCATTTGGTATGAACCAAAGTGCATTCCTTTTATATGTGCCTGG3670.11064981895036437No Hit
TACAGAGGGATTTTTGTATGTGTTAAAAAGACTACTATATCACAATTAAAAG3640.10974532451752758No Hit
TGTAAGGGAGGGCCCAATATTAGTACATAAACAAAATGTAAATTTACTCTGG3630.10944382637324866No Hit
TTTCTAGGGAGATGTTGTAACTTTTCACTGTACAAGCATTGCAATGATTGTG3630.10944382637324866No Hit
TTAATGGGGATACTCATCCACATGAATGTCAGAGTTGCATGGGCCACTTCTT3610.10884083008469082No Hit
ACATATGGGAGAGTGACTAAAATAATAAATAGGAATAACATTAAATGCTGGT3610.10884083008469082No Hit
CAAAGCGGGAAGCTTTTTTTCTTTTATAAGTTTGTGCTTTCTATTTCTTATC3600.1085393319404119No Hit
GTAGACGGGAGGCGGGGCGGGAGCCGGGGGGCGCAGCTAGAGAGCCCCGGAG3600.1085393319404119No Hit
TAAATAGGGGTAGTTAGCTCATTCGTACAGACATTTTCAGTATATGCAGAAA3600.1085393319404119No Hit
CCCATTGTGAATGTTGTCCTGTGTCATCTGTCAGCTTCCCAACAATACTTTG3590.10823783379613298No Hit
TCTACTTCGGGGTGTGAGAACAATGGGAAACTGTAGCTTGCTCCTTCCCACC3590.10823783379613298No Hit
TTGAAAGGGGCCAGGTATATGGTCAATTTTGAAAACTGTTACACAGATGACA3570.10763483750757515No Hit
GTTCAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCC3560.10733333936329623No Hit
GTGAAGGGGAGGCCGAACTTCCGGCTCTCACTGCTAGGGGCTTAAGCGGAGG3550.1070318412190173No Hit
TAGCCTGGGCCCTTTGGGAAACCAGAGCATTGGCTAAACTGGAACCTGAAAA3540.10673034307473839No Hit
ACGCCAGGGAGGTTTACTCACGTCATCCAGCAGAGAATGGAAAGTCAAATTT3540.10673034307473839No Hit
GTAGTCGGGATTTTTCAACATGTGACATCAGAATAAAGTTCTGTATTTTGGT3540.10673034307473839No Hit
GTCGCCGGGCATACTGATTTGAAGCCATATGAATGTAAGCAATGTGGGAAAG3530.10642884493045944No Hit
AACACCGGGACTGGGAAGGATACTCAGAGGTTATCTTGCCTATCTTTCTGCT3520.10612734678618052No Hit
AGGCAGGGAAGATGGGCTTTGGAAGAGGCTTGCGATTTGGGTAATATTGAAA3500.10552435049762268No Hit
TGCCATGGGGCAAGACAAAGGAGGAGGAGGAGAAAGAAAAGAAGAAACAAAA3480.10492135420906484No Hit
GAATATGGGGTTCAAACTGCCTGGTAAGTCACTTTTTTAGATTTCTTTCCTA3480.10492135420906484No Hit
ACTGCCAGGGCACCACTAAGAAAAAAATGTAGAACCATTTGGAAAAATGAAA3470.10461985606478592No Hit
TGTAAAGGGTGATTTAGGAAGATCTGAGCATTTCTTTCATTGTTGGTAAGAT3470.10461985606478592No Hit
GGCGGCGGGTATATCAAAGTCAGTTGAATGAATATGACAATAGACAACAATT3460.104318357920507No Hit
CGTTATGGGATAAAGTGCTAGAAAATCATGTTCCTTGTCCTGAGTAAGAGTT3460.104318357920507No Hit
GTACATGGGTGGTTGAAAAACTGCAGTTGAGAAATCAGATAGCAATATAGAC3450.10401685977622808No Hit
ACTGTTGGGGAAGAGTCTTTAGCAGCGTTCTGTGAAAGCTGTGTTTAAAGGA3430.10341386348767025No Hit
CTTTCTGAGATCTGTGAGATACCGTTAGGCATACTACCATGTGCATAATGAG3430.10341386348767025No Hit
AATTCGGGGGGTTACTGTTTTCATGAAGAAGAAATAGTTACTGCAAATGCTT3430.10341386348767025No Hit
CTCTCAGGGATGTAATCATGTCTTTTAAACTTTTTCTTTGTTTTCTGTTTGA3420.10311236534339133No Hit
AACACCGGGAGATGATAGAAATTGACAGGGTTGCATTTGACATAAAATATCT3410.10281086719911238No Hit
GTAATTGGGCTGTACTTATTGTAGATTCAATGACGCAGTTAAGTCATCACCC3410.10281086719911238No Hit
GCTAAGGGAATAGTAAAAAGAATACCTAAATTTAAAAATGGACAAAGGACTA3400.10250936905483346No Hit
CTGCTGGGATATAGAAATAGTGTGGGCATTTCTTCCTGTTAGGTGGAGTGTA3400.10250936905483346No Hit
AATCCAGGGTACTAATTTCTTAGAAATGCATCACTCTTTTTTCCTTATTTGC3400.10250936905483346No Hit
AGTACCGGGAGTTCAAGTGAATCTGGATAATTGTTCATCTTTGCTTTAGCTT3390.10220787091055454No Hit
TAACAAGGGGAGCTTTTCTGGATTTTTATGATCTGAATGGCATATTTTCAAA3380.10190637276627562No Hit
CAACACGGGACAGCAGAAAAAGTTGTTTATTGTTGAATAAACCTTCTTACAC3380.10190637276627562No Hit
GCCAACGGGGACTAAACTTTAATTGATCCTTCTGTTTCTTAATCTGTTCTTC3370.1016048746219967No Hit
AAACTGGGGTATCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAG3370.1016048746219967No Hit
TCACTAGGGGCAAGAGCAAAGTTTCCGTTGATGAAACAGACATCCCACAACA3370.1016048746219967No Hit
TGGATTGGGAAGAACTTTATATATAAATGTATTGTTGCTCTTTGAAATGGTA3370.1016048746219967No Hit
TGGCATGGGGAATGCTGTTGATGGGAATGCTGGAAGCAGAAACTTTGTCATC3360.10130337647771778No Hit
TCTGTAGGGATATAAATCTGTAATAGATTTTAGAATTGAAAAATTCTTTGTG3350.10100187833343886No Hit
AATCTGGGGAAGGAAGTGTAACTTTGAAATACATCAGTAGTAAACCACGCAT3350.10100187833343886No Hit
GACGAGGGGCAGCTGAGACAGTCCTCCCTGGTGGCATGGCCCTGTTTCCTGT3340.10070038018915994No Hit
TATCCAGGGCGATCTGCAGGTGGGAGACATAAAGTGGAATGATTGACACATG3340.10070038018915994No Hit
GTCACCGGGCTGCCTAAGGGATCAAAGATACACTAGGAAATCAGGGTAAAAA3330.10039888204488101No Hit
CGCAGCGGAGAGTAGATACTACATTAGATTATCACTAGAAAGGACAAATGAT3330.10039888204488101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)