FastQCFastQC Report
Sat 31 Dec 2022
SRR5848277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329152
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGCCGTCT18310.556277950612483RNA PCR Primer, Index 32 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTACGCAATCTCGTATGC17450.5301502041609956No Hit
TTCTAGGGGAAATTATCTGCAGTTAGTTTCTTTGAAGAACATAGTTGGAAGT4800.14582928251993002No Hit
TAAGCAGGGTGTGAAACTTACCCTAGATTTGTAAAGATAATTGTTTCAAACT4550.13823400738868363No Hit
GGTCTTGGGAAGACATCCAGTTAAAATAGGAAAAAATTAACATCTAGAATCT4410.1339806533151857No Hit
TTCTTAGGGCGATAGAATAAGGAAATTGAGCCAAAATCTGCAACTACCTGGC4370.13276540929418626No Hit
AGTACCGGGATTCTTTCTATGATGTTACTTCTTTCTTTATTTAGATATGGGT4320.13124635426793702No Hit
CCTCTAGGGATTTCCAAGAATTCTCCCAATAATATGAGCCTGAGTAATCAAC4310.13094254326268714No Hit
CAAGCCGGGTGCTTGCAAAAGTAAACAGTGAAATAATTAATGTTGGCAAATA4310.13094254326268714No Hit
TTATGCGGGAAGATAGACAATTGATGCATAAGGAAATGTAAGTATATTTACT4300.1306387322574373No Hit
TTTAAGGGGGTCATCATTTAAGGATGAGAGAGGATGGGGAAAGGCAATGTAG4180.12699300019443904No Hit
ATGTAAGGGCAAAAAATTGAAGTATCTAAGAATGCATCTAAACAAGGAGGTG4180.12699300019443904No Hit
TGTGTGGGGGGGCTAACCTTTATTAAACAGATCGCATATTATGATCTTGCTG4170.1266891891891892No Hit
AAAAATGGGACTGTGGTGGGGCTTGAGAATTTGCACGTAATTAATTTCCAGA4140.12577775617343961No Hit
GTAGTCGGGATTTTTCAACATGTGACATCAGAATAAAGTTCTGTATTTTGGT4140.12577775617343961No Hit
TAGGCTGGGAGGATACAAAGTGTGATTTGTTGGAAACATGTCCCAAATTTCT4120.12517013416293993No Hit
ACGCCAGGGAGGTTTACTCACGTCATCCAGCAGAGAATGGAAAGTCAAATTT4080.12395489014194051No Hit
TGAAAGGGGCAGGCTAGTCTCGAACTCCTGACCTCAGGTGATCTGCCTGCCT4060.12334726813144078No Hit
AGAGAAGGGTATCATGATATTCTTCACTAAATTTAGCTGTCCCTAATCACAG4000.12152440209994167No Hit
GACAATGGGAGGATATGAAACTGGGAGCAGGAGTTAGAATCTGTGATAGGAC3990.12122059109469183No Hit
ATAATAGCGGTACTAGGCAGAATTCACTAATGGAGTAGATATGTGGCATTCA3990.12122059109469183No Hit
TATTCGCGGGACTGCAGAAGAATAACCTGACCTTTTCTTTGCATTTGTTTTG3950.1200053470736924No Hit
GCTTATGGGAAACAGAAGATTAGACAAATTCCTTACTCTTAAGTATTTTTTT3920.11909391405794283No Hit
ACAATCGGGACCAAAACAGACACAAGAAAAAATCATGTAGTCTGAATATTCA3910.11879010305269298No Hit
TTTCTAGGGAGATGTTGTAACTTTTCACTGTACAAGCATTGCAATGATTGTG3900.11848629204744314No Hit
CGTTATGGGATAAAGTGCTAGAAAATCATGTTCCTTGTCCTGAGTAAGAGTT3890.11818248104219327No Hit
CGTGACGGGGAAAAACAAATGCAAACAAATTACAAATTTCAAATGATAGAAG3880.11787867003694341No Hit
ACGTTCGGGACTCAGTTTCACATGAGTTTAGGGTTAAATATTCAATGTTTAC3870.11757485903169357No Hit
AGATCTGGGTAAAAGTGAATATTGAAGCTTCTTAAAGTTGGTCTTAATTTTT3840.116663426015944No Hit
AACAAAGGGTCTTGCCAACCAACCATGAGCTCCCAGATTCGTCAGAATTATT3820.1160558040054443No Hit
ACATTTGGGATGACAATCTGTTTTTAAATTGGATTCTATGAAAATGCATAAT3810.11575199300019445No Hit
GGTTCAGGGGACGCTCTTCCGATCTAGCTTCGGGACATATATGAAAGAAGGC3790.11514437098969472No Hit
TCATCGGGGGGAAGAAGATGCCTAATATGTTAGTTTTTCACCCTTGGATCCT3790.11514437098969472No Hit
GTGCCTGGGATGGAAAATTTTATTAAATACTAGTAAATAACTTGTGATCATA3780.11484055998444488No Hit
TTTCGTCGGGGGAGAAAAAGCAGAAGAGGCAGAAGAACTTCGATTAGATCTC3780.11484055998444488No Hit
ACATATGGGAGAGTGACTAAAATAATAAATAGGAATAACATTAAATGCTGGT3760.11423293797394518No Hit
TAAGGCAGGGCAGGACTCTTGCAGGAGAAGCAATGGAGTCAGTGTGGTGTGG3750.11392912696869531No Hit
ATACCAGGGCATTTGGTATGAACCAAAGTGCATTCCTTTTATATGTGCCTGG3730.11332150495819561No Hit
GTAAGAGGGATGGTTTCTATTTGTTTTGAAAAGCAGCTACAACCTTCAAATG3720.11301769395294575No Hit
TTCGATGGGGTGCAGTGGTGTGATCTCAGCTCACTGCAATTTCTGCCTCTGG3710.11271388294769588No Hit
ATTCCCGGGTGGGAAAAGCGATTTTTTTCATGTATTCATTCTGAATAGTTGA3710.11271388294769588No Hit
TGTTGCTGGGATGGGCAACATGTTCAGCTTCGCGGCGGTCATGGGTGCCCTG3710.11271388294769588No Hit
CAAAGCGGGAAGCTTTTTTTCTTTTATAAGTTTGTGCTTTCTATTTCTTATC3700.11241007194244604No Hit
AGCATTGGGAGTGCTATAAATTTCCCTCTTAACAGTGCTTTAGCTGTGTCCC3690.1121062609371962No Hit
AACACCGGGAGATGATAGAAATTGACAGGGTTGCATTTGACATAAAATATCT3680.11180244993194634No Hit
TTAATGGGGATACTCATCCACATGAATGTCAGAGTTGCATGGGCCACTTCTT3670.1114986389266965No Hit
CAGCCTCGGGATTGGCTTTCTTTTTAGTCTTTGCCACTTTGTAAAAGGAAAA3660.11119482792144662No Hit
TAACGAGGGTGCCTCATGCATTGCATACTCTTATTTAAGTAGGAGTCAGTTA3650.11089101691619677No Hit
CTGTTTGGGGCACAATGGTAAGATGATCCATACAGGTTTTATACATATTTCT3650.11089101691619677No Hit
TACAGAGGGATTTTTGTATGTGTTAAAAAGACTACTATATCACAATTAAAAG3620.1099795839004472No Hit
ATCCATGGACCAGCACCGCTGGGACTCTTCTAGCAAGCCATGCTTAACTTCC3600.1093719618899475No Hit
GTACATGGGTGGTTGAAAAACTGCAGTTGAGAAATCAGATAGCAATATAGAC3600.1093719618899475No Hit
GTACTGCCGGGAAGATTTTGCAGATTTATCAATTTGGGTTTTTGTACTGAAG3600.1093719618899475No Hit
CAGGCGGGGTTGGCATAGACATAAAGGATAAAAGGAAAATAACCGTCTGCCG3600.1093719618899475No Hit
TATCCAGGGCGATCTGCAGGTGGGAGACATAAAGTGGAATGATTGACACATG3590.10906815088469765No Hit
TATACAGGGAAGAAAAGTTCAAAAACTAATTAATTTGACCTTGAGTTTTAGT3590.10906815088469765No Hit
AGCAATGGGAACACACACACACACAAAACAACAGCAACAACAACAGAACAAC3590.10906815088469765No Hit
CAAATGGGGCAACAAAACTTGATCTGAAATCCCTATAATAGGTAGCTGACAA3580.10876433987944781No Hit
TAAATAGGGGTAGTTAGCTCATTCGTACAGACATTTTCAGTATATGCAGAAA3580.10876433987944781No Hit
CCCCAGACTTTAGCCAAAATTTCCCCACGAACAGAGATTCTTAAATATCTCC3570.10846052887419794No Hit
TGTAAGGGAGGGCCCAATATTAGTACATAAACAAAATGTAAATTTACTCTGG3570.10846052887419794No Hit
ACTTGCGGGTGGGTGTGAAAGTGGTGGATCCAGCAGGAAGCCCAGCCTCACA3560.10815671786894808No Hit
TTGAAAGGGGCCAGGTATATGGTCAATTTTGAAAACTGTTACACAGATGACA3560.10815671786894808No Hit
TAACAAGGGGAGCTTTTCTGGATTTTTATGATCTGAATGGCATATTTTCAAA3550.10785290686369824No Hit
CAACAAGGGACCTGACACTAGGGGCTCTAGAGAGGCAGAGAGAGCACTCACA3540.10754909585844838No Hit
GACCTGCAGTTTGAAAAACACTGTAGTTAGTTTTATCTCCACCTCAGGCCAG3540.10754909585844838No Hit
CAAGCTGGGATTGAGCTCATCCATAAAACAAAGAAGCTATTGCAGGCTGCAG3540.10754909585844838No Hit
TCGCATGGGGAATGAACAAAATACTATAGAAAAAGAAGAGTTCATGTTGAAA3530.10724528485319851No Hit
TTGTGCTGGGATGTAATAAAATGTTAGGTAGGACCTAACATTATATTATGCT3520.10694147384794866No Hit
TTGCTAGGGGAAATGATACCTATGGAGTGTGATTCATTTTGCAGTGACCAAA3510.10663766284269881No Hit
TCATTTGGGAGTTTCCAAAAAACAAATCTTTATATATACCTTTCCAGTGCCA3500.10633385183744896No Hit
CTGCTGGGATATAGAAATAGTGTGGGCATTTCTTCCTGTTAGGTGGAGTGTA3500.10633385183744896No Hit
ACGCAAGGGAGCTGGTAGGTAGACAGCAAGAATCCAGGTCTTCTGACTCCTC3500.10633385183744896No Hit
GGCGGCGGGTATATCAAAGTCAGTTGAATGAATATGACAATAGACAACAATT3490.10603004083219912No Hit
TGCCAACGGGCACGACGCTCTTCCTTTTCTAGAAGCGGGGGTAATAAACTGA3490.10603004083219912No Hit
GTGAAGGGGAGGCCGAACTTCCGGCTCTCACTGCTAGGGGCTTAAGCGGAGG3490.10603004083219912No Hit
ATTTCCGGGACACATAACATTTTTTAGTTCATTCTGCCTTTCCTATTTGTTC3490.10603004083219912No Hit
GTCGCCGGGCATACTGATTTGAAGCCATATGAATGTAAGCAATGTGGGAAAG3490.10603004083219912No Hit
TGGCATGGGGAATGCTGTTGATGGGAATGCTGGAAGCAGAAACTTTGTCATC3480.10572622982694925No Hit
AAGACTGGGCCAACCAACCACCACAACTTCTTATACATTTGGGACATGAGCC3480.10572622982694925No Hit
TTTACAGGGACTCATGACAAATCAACTGTGACCCTCGCTCCTTCCATTTCTG3480.10572622982694925No Hit
GTTACTGGGAGCTGTTGTTGTTGGACTTGTGTTCCAATGAGTGACTAGGAAA3480.10572622982694925No Hit
GCAGCGGGGGCACAAAAGTCTCAATGTTCCATTCAGTCTGCAGCTATTCACA3470.10542241882169939No Hit
GAAGTTGGGTATGATGTGGATATGATAGAGATAACTACAACTTTTTGTCTGA3460.10511860781644955No Hit
ACGTTTGGGACTTGACCTCATAAATACTGGTCCGCACTAGAGTGTGGTAGCA3460.10511860781644955No Hit
GGCGCTGGGACATCAATCCAGACTATTAAAAATTTTATGTGAGGAAATGGGT3450.10481479681119969No Hit
GATGCTGGGAGGAAAAGAAGAGTTTCAAAATGCTTGAAAAGTAGTATGTGGT3450.10481479681119969No Hit
ATGAAAGGGACCTCAAAGAGACTTTTGGGGTTGGTTATTGATAGATAACTGT3430.10420717480069998No Hit
ATGCGTGGGCATAGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAA3430.10420717480069998No Hit
GCTTCTGGGAAAGTATAAAATGCTTAAAGATCCTTACTTTTTATTACCAAGT3430.10420717480069998No Hit
AACACCGGGACTGGGAAGGATACTCAGAGGTTATCTTGCCTATCTTTCTGCT3430.10420717480069998No Hit
AGATCAGGGTCTTTCCAAGTATTTGGTAGTTTTGTCAAGACTCGATACACAG3410.10359955279020028No Hit
CCATGAGGGATAACATACATGTGCAGAAATGTATTCAAATGAAAGGAAGCAT3400.10329574178495043No Hit
GGTTTGGGGGAGATTAAGACTCAGACATCTTTGGGAGGCCATTTTGTATCTG3400.10329574178495043No Hit
TGCTATGGGAACAGTTCTAATGGTAACCATATATTTTGGCCCATGGGCCATT3400.10329574178495043No Hit
AGGCAGGGAAGATGGGCTTTGGAAGAGGCTTGCGATTTGGGTAATATTGAAA3390.10299193077970056No Hit
CTTACAGGGATCTCTTCCAAAAGTCAACTTTTTGTTTCATTAATTCTTTGCA3380.1026881197744507No Hit
CGCAGCGGAGAGTAGATACTACATTAGATTATCACTAGAAAGGACAAATGAT3380.1026881197744507No Hit
GAAGCAGGGATTTCAAGAGAGTGGCAGGTCTGTTCCTGGTAAAATTTTAACC3370.10238430876920086No Hit
GCTAACGGGAAATGTATGTGCATATATCTGTATCTATATATATATAGCATGT3360.102080497763951No Hit
GTAATTGGGCTGTACTTATTGTAGATTCAATGACGCAGTTAAGTCATCACCC3360.102080497763951No Hit
TTCCGTGGGGCCTAAATAAATGAGCTGTTGGAAGAAAAGAATCACAGTACTT3340.10147287575345129No Hit
CTTTCTGAGATCTGTGAGATACCGTTAGGCATACTACCATGTGCATAATGAG3340.10147287575345129No Hit
TCACTAGGGGCAAGAGCAAAGTTTCCGTTGATGAAACAGACATCCCACAACA3340.10147287575345129No Hit
AAACTGGGGTATCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAG3330.10116906474820143No Hit
TGCCATGGGGCAAGACAAAGGAGGAGGAGGAGAAAGAAAAGAAGAAACAAAA3330.10116906474820143No Hit
AAAAAGGGGCAGTTCTGTGTGGCTGGGGAGGCCTCAGGAAACTTATAATCAT3320.10086525374295159No Hit
GCTGACGGGCAACTTGGATTGTTTAAACTTTCATTCACTAGATATTCATGAA3320.10086525374295159No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)