FastQCFastQC Report
Sat 31 Dec 2022
SRR5848243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121243
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGC7470.6161180439283093No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGCCGTCT6860.5658058609569212No Hit
ACGCGTGGGCAAGCGGAACCAGCTTCATGATGAGGTTCATCAGTGGCGACGG2540.20949663073332067No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTTATACA2090.1723810859183623No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTT2000.16495797695537062No Hit
CGATTTGGGATTACCACCTCTCTTTGTAATTTACTTTACTTGTATGAATTTA1960.1616588174162632No Hit
GCTAGGGTCCTCTCTTCAATGTATTTAGATCCACCATAAAGTAAACTGCCTC1700.14021428041206502No Hit
TCGCAAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1580.1303168017947428No Hit
TCGCTTGGGATTTTCTTGTTTAGCTCTGTGACCCTCCATGCTACTTTTCCTA1560.1286672220251891No Hit
GGCATGGGGAGATCAAAGACCAAGCTCACCAATTCGACATCAGGGAAGGAAT1560.1286672220251891No Hit
GTCTATGGGGCTGGTGACCAGCAAGTGTCAGGCTGACGCCCAGGCCCAGCAG1520.12536806248608168No Hit
TAACTAGGGCATCTGAACTAGGTTGATCCTGGAATAATGAAAACACTTCACT1510.12454327260130482No Hit
GGCCTAGGGACTTGAAGGAACCTCAGCTATGATTTGGTCCAACCTCCTAACT1510.12454327260130482No Hit
GCGTTTGGGGGAAAAAATGAAAATAGGAAAAAAGGAAGTGAAAGAGATAATA1480.12206890294697427No Hit
CTTATTGGGAAGGAGAATAAAAAATTAGAAAAGATCTTTTTTATAGTTGCCC1470.1212441130621974No Hit
AACCGAGGGGGAGTAACTGAACACAAAGTTTCAAAGGGATGCATGCAATTCA1450.1195945332926437No Hit
CAGCAAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1430.11794495352309No Hit
TCCAGAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1430.11794495352309No Hit
TATGTTGGGGGCGCTCGCCATGGCGGTTTGGGCGGCGACGTGAGCGGCTCCG1420.11712016363831315No Hit
CATAGCGGGGGAGGCTGAGGCGGGAGGATGGCTTGAACCCGGGAGGCGGATG1410.11629537375353628No Hit
GCATAAGGGAAGATTATATGAAAATGGAGTCAGTGATTTTTAGTAAAAGCTC1410.11629537375353628No Hit
CTAGAGGGAGACCATTAGAAAGCTTTTTCAATAATTGAGGTGAGAAATGATG1400.11547058386875944No Hit
ACTAGCGGGGATGCTGGAAACTAAACCAGCAGGGTCCGGGGAAAGAGAAAAA1400.11547058386875944No Hit
CTTCTTGGGATAGTAAGAGAAATATGAGTGCTATCAATACATGAATATTTCC1400.11547058386875944No Hit
CACAGAGGGACCCCTTCTTCTCCCGTGAGAAGGGGAGAGAGCACCCCTTCTC1390.11464579398398259No Hit
CACTCTGGGAAAATAAAATCCTAACTTTCCTAGTTAGAAATATAATTAACTT1380.11382100409920573No Hit
AGAGCAGGGTAGATATGTGGCATTCAACAAAGAGAGGTTCCAGAATTTTGGC1370.11299621421442888No Hit
TACAATGGGTACCAGGACGGGGAGCTGCAGGGCTCCGGTGAGAACCTGGCTG1370.11299621421442888No Hit
CAATTCGGGATTCTAGCCTAGTTGATAATTTTTATTTAAAAGGTGGTCCCTG1370.11299621421442888No Hit
GTTCCAGGGGACGGACGCCGTCCCTCCTCGGCGCGGCCTGAGCGCCCGGCCC1370.11299621421442888No Hit
AGAAACGGGGTGTTAATTTTTTCTTGTTCAAAGGACTTGGTTTATTTAAATT1360.11217142432965203No Hit
GCTAGAGGGACCTCCAGACCGTGGTGTCTGGCCTCCATTTTTGTCTGTCATT1360.11217142432965203No Hit
CAAGCCGGGAGGAAATAAGAAGTGTAAGATATCTACAGTCAAGAAATTTAGA1340.11052184456009832No Hit
TGTGGCGGGGATCCCAATTTCCTGGTGGTGGAGAAGGATACAACCATCAATG1340.11052184456009832No Hit
CGCATTGGGGAAAAGCGAGTGAGTAGGAGTTTGTCACACTGCCATGTACCAG1330.10969705467532145No Hit
CTACCTGGGACATCTGGCAAGACAACTTCAAGTACAGATGGACCCAAATTCC1330.10969705467532145No Hit
TTTTGCTGGGGCCCTCAGACACAGAGATCATTGTCAACCAGGCCTGTGGGGG1310.10804747490576776No Hit
CAGTCGGGGCTCCCTCCATTCGGACAGCTCCTACCCACCGGATGCGGGCCTG1310.10804747490576776No Hit
CATTTAGGGGTGCATGTGTATAAATTATTCCTTTTGAGTTTTGACTTACTGT1300.10722268502099092No Hit
AGTCAAGGGATTGATTTAAATATTTGGAAATGACTGTTTCTGGTATTAATTG1300.10722268502099092No Hit
TTTCTAGGGGAAAAAGAAATTAAGAAAAAATTCCATTTACGCTAACTAAAAC1280.1055731052514372No Hit
TCTAAGGGGATGGCCGATGAAAATAAAAATGAATATGCTGCACAATTACAAA1280.1055731052514372No Hit
CATAGCGGGAATTTACTACAATAGTAAATATACCAGTAAATTAGTAGGAAAT1270.10474831536666034No Hit
CAGTCTGGGACACTTAATTATGAATCTACTGGCTCCTGGAAAAAAATACTGT1270.10474831536666034No Hit
TGGACTGGGGTGAAGGTTTAGCTGTAAGTAAACTCTAGTTCTTTCCATTAGG1270.10474831536666034No Hit
ATTGCCGGGAACAGTACTGGCCAGTCTCCTCACCACAAAGTGCAGAGAAGTG1260.1039235254818835No Hit
TACCTAGGGGCAGGTTACTGAGACAACCAGTACCAAGGACTTGTTCTGGAGA1260.1039235254818835No Hit
ATGCATAGGGTGGACATTTTGACATTTGGAGAAGAAAGATGGCACACTAACC1240.1022739457123298No Hit
GGCCAAGGGACTGAGAAATTTGACGACTGGGCTAGCAACAAGATACCTACCC1240.1022739457123298No Hit
GTCAACGGGGTATCTTAACTATTCTTCCTTGGCCGTTAGCTGGCACTTAAGG1240.1022739457123298No Hit
ACTCAAGGGGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAA1230.10144915582755293No Hit
CTAGTCTGGGATCCAATCTCGTGTGTAATGCACTCGTTCAGTCACTCTTGGG1220.10062436594277609No Hit
ATTTAAGGGGAGAGAAGCTTTGAGTAAGTGTCTTTTTCTCTCCTAAAAGAAA1220.10062436594277609No Hit
AAAAATGGGACTAATTTGATTTCAATTAATAGAGAACTAAAATACAAATCAG1220.10062436594277609No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)