FastQCFastQC Report
Sat 31 Dec 2022
SRR5848242.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848242.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120328
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGC7590.6307758792633469No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGCCGTCT6000.49863705870620306No Hit
ACGCGTGGGCAAGCGGAACCAGCTTCATGATGAGGTTCATCAGTGGCGACGG2730.2268798617113224No Hit
GCGTTTGGGGGAAAAAATGAAAATAGGAAAAAAGGAAGTGAAAGAGATAATA1980.164550229373047No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTTATACA1970.16371916760853666No Hit
CGATTTGGGATTACCACCTCTCTTTGTAATTTACTTTACTTGTATGAATTTA1760.14626687055381957No Hit
GGCATGGGGAGATCAAAGACCAAGCTCACCAATTCGACATCAGGGAAGGAAT1680.13961837643773686No Hit
CTAGAGGGAGACCATTAGAAAGCTTTTTCAATAATTGAGGTGAGAAATGATG1670.13878731467322653No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTT1670.13878731467322653No Hit
GGCCTAGGGACTTGAAGGAACCTCAGCTATGATTTGGTCCAACCTCCTAACT1660.1379562529087162No Hit
GCTAGGGTCCTCTCTTCAATGTATTTAGATCCACCATAAAGTAAACTGCCTC1570.13047669702812315No Hit
CACCTGGGGACCCTCCAGAACATCCTCCTCGTCTACCACTTTCCTCCACATC1530.1271524499700818No Hit
CTTATTGGGAAGGAGAATAAAAAATTAGAAAAGATCTTTTTTATAGTTGCCC1520.12632138820557143No Hit
CAGTCTGGGACACTTAATTATGAATCTACTGGCTCCTGGAAAAAAATACTGT1510.1254903264410611No Hit
CAGCAAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1510.1254903264410611No Hit
TGCCATGGGGAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGC1470.12216607938301974No Hit
CATAGCGGGGGAGGCTGAGGCGGGAGGATGGCTTGAACCCGGGAGGCGGATG1460.1213350176185094No Hit
ACTAGCGGGGATGCTGGAAACTAAACCAGCAGGGTCCGGGGAAAGAGAAAAA1460.1213350176185094No Hit
AGAAACGGGGTGTTAATTTTTTCTTGTTCAAAGGACTTGGTTTATTTAAATT1410.11717970879595771No Hit
CCAACTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1400.11634864703144739No Hit
CACAGAGGGACCCCTTCTTCTCCCGTGAGAAGGGGAGAGAGCACCCCTTCTC1400.11634864703144739No Hit
TCCAGAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1390.11551758526693705No Hit
TGCTGTGGGCGATCTTGTGGAGGGATACTCCATAGAAACTAAACTAAAATAG1390.11551758526693705No Hit
GCTTGAGGGGGAGGAGGCCCTGGAGCTGATCAACGGCTACATCCAGAAGATC1370.11385546173791636No Hit
TAGCTAGGGAATGAAAAAGTCAAGAAAATAAAAATCTAGGTGAAGAAAGACT1370.11385546173791636No Hit
AGAGCAGGGTAGATATGTGGCATTCAACAAAGAGAGGTTCCAGAATTTTGGC1360.11302439997340602No Hit
TCATGGAGGATGACTGAATTTTGCTTTTATGGCTTTTGCTCTCAAAAAGTAG1350.1121933382088957No Hit
CAATTCGGGATTCTAGCCTAGTTGATAATTTTTATTTAAAAGGTGGTCCCTG1350.1121933382088957No Hit
GGCCAAGGGACTGAGAAATTTGACGACTGGGCTAGCAACAAGATACCTACCC1340.11136227644438533No Hit
TCGCTTGGGATTTTCTTGTTTAGCTCTGTGACCCTCCATGCTACTTTTCCTA1330.110531214679875No Hit
CGAGTAGGGATCTCACTGTAAAGGAGTATTTGTCACAATAAGCCTCTTATTT1330.110531214679875No Hit
AGCAACGGGGTGGGAGGCCGATTCTAGTGCGCCTGCGTGGCCGCGAATCACC1330.110531214679875No Hit
GCTGTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1330.110531214679875No Hit
TCCTAAGGGGAATTTTAAATGAAACCAGTCACAGTTGTATGATTTTCTGCAG1300.108038029386344No Hit
GGTAAGGGGGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCC1300.108038029386344No Hit
GTAAATGGGATGAAAACGAACTTATATGACATGACTCTAGTTTTATCACATT1300.108038029386344No Hit
CTTTCGGGGGGGCAGTCCTGACAATTCGATCTCACACCAAGCTCACCTGAAA1290.10720696762183364No Hit
TCACCGGGGATTTTGGTCTGAGGTTTGAATTGCAAAGAAGAATAAGAAGTTG1290.10720696762183364No Hit
TGGTGAGGGATCCCTGACAGGGATGGCTCCGACCCATACCAGGAGCCCAAGA1290.10720696762183364No Hit
AACCGAGGGGGAGTAACTGAACACAAAGTTTCAAAGGGATGCATGCAATTCA1290.10720696762183364No Hit
GTCAACGGGGTATCTTAACTATTCTTCCTTGGCCGTTAGCTGGCACTTAAGG1290.10720696762183364No Hit
GTTCTGGGATACCTAGCTCCAAGATCTTGGTTGCTAACACCATTCTCCAATA1270.10554484409281298No Hit
TCACAAGGGCTAGTTATAATGACTTAAAATTCATGGTCCCAAACAGCAATTA1270.10554484409281298No Hit
CGCCTTTGGGGAAACATTTTGGGCCTACGCATTAATGATTTGTGATAGACTA1260.10471378232830264No Hit
GTAATTGGGATGAAATGTAAATATATTTTTTCACAGGAGTAATTTTATAACA1250.10388272056379229No Hit
CATTTAGGGGTGCATGTGTATAAATTATTCCTTTTGAGTTTTGACTTACTGT1250.10388272056379229No Hit
TCGCTTGGGGAGTCAACCATCCCATGTGGTGACTGATAATTGGTACATGTTT1240.10305165879928195No Hit
TTTTGCTGGGGCCCTCAGACACAGAGATCATTGTCAACCAGGCCTGTGGGGG1240.10305165879928195No Hit
ATAACGGGGAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCC1240.10305165879928195No Hit
TGGACTGGGGTGAAGGTTTAGCTGTAAGTAAACTCTAGTTCTTTCCATTAGG1240.10305165879928195No Hit
CGTTCGGGGATAGTCCAAACAAAAAAGAAGGCTTTTTCAAAAAACATTAAAT1240.10305165879928195No Hit
CATAGCGGGAATTTACTACAATAGTAAATATACCAGTAAATTAGTAGGAAAT1230.10222059703477163No Hit
TAACTAGGGCATCTGAACTAGGTTGATCCTGGAATAATGAAAACACTTCACT1230.10222059703477163No Hit
GCGCTTGGGGATGTCCATTGCTGTGTGACAGCTTGGAATTTAATTTATTAAA1230.10222059703477163No Hit
ACTGAAGGGTCAGGCCTTTAAAAAAAGAAATGTTATACTCTTCTGATGGTTA1230.10222059703477163No Hit
CACTCTGGGAAAATAAAATCCTAACTTTCCTAGTTAGAAATATAATTAACTT1220.10138953527026129No Hit
GCAAATGGGGTCCCCCCTCCATCTCCAAAAAAACAAGGTGTCATGAATAGGA1220.10138953527026129No Hit
TTCTTTGGGCGGCCGCGTGTCCCTCCTTACAGGGGATGAAATGACCTGGGGA1220.10138953527026129No Hit
TACAATGGGTACCAGGACGGGGAGCTGCAGGGCTCCGGTGAGAACCTGGCTG1220.10138953527026129No Hit
CTAGTCTGGGATCCAATCTCGTGTGTAATGCACTCGTTCAGTCACTCTTGGG1210.10055847350575095No Hit
GCAGATGGTAACTTTTATTTCCTAATATATAGTTGCTACTCTGGGAATCATA1210.10055847350575095No Hit
GATCATGGGAATTCAGAAGAGCCTACCACAAAATTTGACTTCCATGCCTGCA1210.10055847350575095No Hit
TCAATAGGGCATCTGGGAGTATAGACTTAGGGTTTATCTATTTTTTTTTTTT1210.10055847350575095No Hit
TATGTTGGGGGCGCTCGCCATGGCGGTTTGGGCGGCGACGTGAGCGGCTCCG1210.10055847350575095No Hit
TTTCTAGGGGAAAAAGAAATTAAGAAAAAATTCCATTTACGCTAACTAAAAC1210.10055847350575095No Hit
GTCCATGGGATGTCTCTTGCCAAATTTGACAAGTTTTCAGCCATTATTTCAT1210.10055847350575095No Hit
TCTTGGGGTCACTATGGCCGGCAGACGCTTCCTCTTTGCTGCTTGCTTTAAG1210.10055847350575095No Hit
CAAGCCGGGAGGAAATAAGAAGTGTAAGATATCTACAGTCAAGAAATTTAGA1210.10055847350575095No Hit
ATATGAGGGAATGTATTACTAGCATAGACAATATCAGTATGGAAACCCCAAA1210.10055847350575095No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)