FastQCFastQC Report
Sat 31 Dec 2022
SRR5848240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122549
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGC6690.545904087344654No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGCCGTCT3680.30028804804608766No Hit
CATGTCGGGATGTGAGTTTTGACAAGCAGAGGTTTAAGTTTGGACAAAGTTC2130.17380802780928445No Hit
GTAGGCGGGGGTGTATGGATTTTAAGAAAGGAATCTAGCCAATGAGGTCCAA2040.16646402663424426No Hit
AACTGAGGGTCTTCCGATCTGCCTATGGGGGAAGGAAAGAGAGAGAGAAAGA1950.15912002545920406No Hit
ACTACCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1920.156672025067524No Hit
CCTCATGGGTCTTCAAGCAATGGGCCTTGTACCAAAGGACCTCATGGCTAAT1870.15259202441472391No Hit
TTTTCGGGAAGGAGTCGTGGCTGCTTGGACAGGCTCAAACCAAGAGAGCCTT1850.15096002415360385No Hit
AGACTAGGGCAGTTATCAATGCGATAGACAGTAGACGAATGCAGGATACAAA1850.15096002415360385No Hit
ACCACAGGGATTAGTTAAAATGGAAGGTGTTAGTTTAACAAACTGAGAGTCA1830.14932802389248384No Hit
TTCATTGGGAGAGAGCGAGTGAGCAAGCGAGCAGAAAAGAGGTGGAGAGGGG1820.1485120237619238No Hit
TGGTCTGGGGGCATGTCTTCCTTCTGGGAGGGTGTGCAGGCCTTTAAACTGA1820.1485120237619238No Hit
ATGCACGGGACAAGCTGTGCAAACAAAGCAAGCAACTGCATTTCTCTGTCTC1820.1485120237619238No Hit
TCTAGCGGGTCCAAGAAAACAAAAAACAAGAAAGATACATCTATTATAAATA1820.1485120237619238No Hit
GAGCACGGGACTGTCCCAGCTCACTGAAAGGTCATAATCCAGGATGAAACCA1810.14769602363136378No Hit
CACAGGGGGGTTTTATAAGGGGCATTCCTCTTTGCTTGGCTCTCATTCTTCT1770.14443202310912368No Hit
GCACAGGGGATGATCGTGAAGTCTTTTACGTATTGAAAATTCGTTTGTTTCG1760.14361602297856368No Hit
AGATAGGGGCCACCAAATCTGACTTTTACATTTGGTCTCAGAATTTAGGTTC1750.14280002284800367No Hit
ATTGTCGGGACCGCAACATCTGGAAAAATCTGCTTATCCTGGGCTTCACCAA1730.14116802258688363No Hit
GAACCTGGGATTATAACCATAGAATAATCACTAAAAAGTACAGAGAAATATA1710.13953602232576356No Hit
TAGCTTGGGATTATCCATACATGTCAGCATTCCCTGGCGGAGTATGATGTAA1710.13953602232576356No Hit
TAATGAGGGATTTTTATAGGGACAGATGATCAATCTTTCTTTTAGGAAATTT1700.13872002219520355No Hit
GATAGGGCTTGGTCCTTGGAAGACCTATATTCTCTGTAGTTACTGAAAACCG1700.13872002219520355No Hit
GACGTTGGGGTCCATTTACATTTATTGATTTCATAAATGTGTGTGGTCCTAT1680.1370880219340835No Hit
ATGCCAGGGTGGTGGTACCAGGAGACGATAAAGTAGAAAGTTGGAGTCAAAC1670.1362720218035235No Hit
TTGGTCCGGGGGTCCGCGGCGGGGAGTTGTTGCTGCCGCGATGCTGGTGGCG1670.1362720218035235No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTTATACA1650.13464002154240345No Hit
AGCAACGGGGTTGGAATATACAGTTTGTATTTGTCTGCTGTGAATCATAATC1650.13464002154240345No Hit
ATGTGCGGGGATTTTTTTGGAGGGTTGTCCAGTGTTTCCTGAAAATGCTTTT1640.13382402141184344No Hit
TTCCATGGGGTATTTTAATTTTGTATCTGATACATAAGCATGTTTCCTGACT1640.13382402141184344No Hit
TGCTGCTGGGATGAAACGTTTCCTGTATTCTAATCTATTTTGAAACATTTTG1630.1330080212812834No Hit
AAACCTGGGCACCTGCTGATTTTAGAGTCCCAGGGCCTTGAGTAAATATAGG1610.13137602102016335No Hit
GCGCAAGGGATTTGTTTTAATTTAAACAAAACTATAATCAACTTGCTTATCT1580.1289280206284833No Hit
ACTTTAGGGAAACAGGGTTGAAGACCAAATGTATATTTCACAGTATCGTACT1570.12811202049792328No Hit
TAATGCGGGCCAACAACTCGTCCATCGAGCTGGAGAAGCTGGCCAAGAAGCA1570.12811202049792328No Hit
TATGACGGGAACTGGAATTAGAGTCTGGGTCTCCCAACCACTGATTCTGCTA1570.12811202049792328No Hit
CTGTAGGGGGACAGCTTAGGCACTCCTAAGAGGAAAGGTCTGTGGAGATGAC1560.12729602036736326No Hit
ACTTTGGGGACTAATTTTCAAACTAAAGCTAATTATAACTGCTAAACTTGAA1560.12729602036736326No Hit
AACAAGGGGATATTCTGTGTTATGTAAATACCTAAATTTGACTTGAAATGAT1550.12648002023680324No Hit
AGCAATGGGGAAAACCAAGTGTGGGGAGCCGTCGCGCCCCGCCGCCTGCCGA1540.12566402010624322No Hit
TTGCGCGGGGGGAAGATGGACATTGTGTATCTCCAATTGTGATATCCTAAGA1540.12566402010624322No Hit
TTAGCAGGGGTGGGAATTCAAAATGAGATTTGTGTGGGGACATGGCCAAACA1540.12566402010624322No Hit
AAGATGGGGATGTATAATAATTTAAGTGGTATTTCCGCTTCCTTACTCAGTT1530.12484801997568319No Hit
CTTGCGGGAACTTTGGTATTTTTTTCTGAGATTTATAAGGTAACAGATTATT1530.12484801997568319No Hit
AGCTCCGGGACAAAAACAAATGAAAGAAGAGGATTTTGTTTTATCACATATA1520.12403201984512319No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTT1520.12403201984512319No Hit
GATTCGGGGATTTTGTCTATTTTTCTGTTTTATGTGAAAACCTTCATTTATA1520.12403201984512319No Hit
TTCAAAGGATTGTCTGGATTTGTTCTGGTTTTCAGGTGCAATGATTGCTGAG1510.12321601971456315No Hit
CAGACGGGGAAGCACACAGTGCGAAAACTCTTGCTGATACGCGATATTGATT1500.12240001958400314No Hit
AACGTTGGGCCACTGCAGTGGCTGGAGAGTGACCTGAGTGCTACTTGAAGAT1500.12240001958400314No Hit
AGTTTAGGGGTGTCTGTCTTGTGATGAAGCACATTATAATACTTTAGACTTG1500.12240001958400314No Hit
GCACTTGGGATCTAGGTATCTGCTATTTTCATCTTTTGGCTTGTTAAACAGT1500.12240001958400314No Hit
AGAAAAGGGATTCAAAATAATGTTCTTAAAGAAGTGCAACAAGATACAAGAA1500.12240001958400314No Hit
CAATAAGGGATGTCCACATCTTGTCCCACTGGAAGGTCTTCAGGGCCAATAA1490.1215840194534431No Hit
GTAGACGGGTAATAGGTATGTGTATTAGTCTTCCAGTGCTGCACAGCAATGT1490.1215840194534431No Hit
GCTGCCGCCATCGTGCCAGCCCCTCGGGTCTCCGTGAGGCCGGGTGACGCTC1480.1207680193228831No Hit
GCTCATGGGTTAGTTGTACAATTGAAAATATTCCTTATTATAGAAGTAAAAA1480.1207680193228831No Hit
GTTTTAGGGAATTAAGGTAAAAAAAATTACATAAAACATAAGCACATCAAAT1480.1207680193228831No Hit
ATTGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1480.1207680193228831No Hit
CTCACAGCGGGATTGACACCGGTGTATAAGTATAAATTTAAATGAACTAATT1470.11995201919232307No Hit
ACCAAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1460.11913601906176305No Hit
TATCGGGAACTGGGTTAAGCTGGTGATGATGATATAATTCCTCTTTTGTAAA1460.11913601906176305No Hit
TCAACTGGGCATGGTTCAGACAACAGAAAGCTGAATATGCCCCTGTGGCAGC1450.11832001893120304No Hit
TGACCGTGGGCTTCCCAGCACCTTGTGCCCTTGCCAGTGAACGCAGGGTTTG1450.11832001893120304No Hit
ATATATGGGATGAGCTGATGTCAATGCTATGGACTGCACAAAAGTCTCAATG1450.11832001893120304No Hit
ACTTAGGGGGTACGGGTAATACTGATAGCGATATGTTCAATGATTAGTAGCT1450.11832001893120304No Hit
AGATGTGGGAGCCTTCTGAAGAAGCAGTTTCCTAGGACAAGTGTCTTGTCTA1440.11750401880064301No Hit
AACATAGGGACTGCAAGGATCCTAACACACACTTAAAATCAAGAGCCAAGGA1430.11668801867008298No Hit
AGATATGGGAAAGATTAAAAGGAAAGGAAATTAATCGGAATTTTGACTATAA1430.11668801867008298No Hit
GTCTGAGGGTATTTACAGTTAATGATCAATTCACATCCAAACTACACAGAGT1430.11668801867008298No Hit
ACTTGAGGGGATTTGCAATTAGATGTTTCCATTCTTGAAAGAAAAAAGCTGC1400.11424001827840292No Hit
GACAACGGGTAACCTGGGAAGTGGAACCGGATATTTAAGTACAATGGTGGGC1400.11424001827840292No Hit
CCCACTAGATGCACCCACTACTTCTGCTGATACTCCATTAGCTAATGCTTGC1400.11424001827840292No Hit
TTTCACGGGAGGAAATCATAGATGCTGTAGACCATGTTGAGTTGGATTGTGT1390.11342401814784289No Hit
ATGCAGGGGGTGGTTTGTCCCGTGTAAGGGAACAGGTGTGGGGAAGAGGTGG1390.11342401814784289No Hit
TTTGTAGGGCAAGGTTAATTTGTATAAATTTAATTTTGTTATAAGAGGCTGT1380.11260801801728289No Hit
TACTACGGCCACTACATAATAGGGCTCAGCCCAGGCAGCCAGCTTTGGGCTG1380.11260801801728289No Hit
ACACAAGGGAAGTAAGTTTATTTTTGAGGAATGTTGTTAAAAATGTCTTTAG1380.11260801801728289No Hit
GTGAGGTGGGAGCTACAAATGCCTATTTATTCCCTAATGTACCTGAACACTA1380.11260801801728289No Hit
TTGTGGGGGGCTTGGACGTGACCCTGGCACCCAGGCAGGTGGATCCCAGCTG1380.11260801801728289No Hit
TCGCGACGGGGCTGCTGTAGGTTTCATTCTCAACATCATTTTGTCCTTTCCT1380.11260801801728289No Hit
CTTGTCGGGATCTGCTGTTAATTACACATGCTGTTATGGGTTTTAACCATGA1380.11260801801728289No Hit
AGACATGGGATCTTTTATCGGGGATGTGGTAATAGAGCAAAAAAAGTTAGTT1370.11179201788672287No Hit
TGAAGCGGGATCCCTCTCTTACTTCTTGTATTTCAGTGTTAAATAGAATTGC1370.11179201788672287No Hit
GGTCTTGGGAACTCAGAAGAGAAAAAATGGAGCCAAGGATGCTAACTTGGAC1370.11179201788672287No Hit
AACGAGGGAGCAGTCAAAAGGTAAGGTACCTTTATTCAAAGGGGCCTTTACT1360.11097601775616284No Hit
TGACTGGGGAGGAGCATTGTCTCAAATACTGAATTGGGTCACTGGGAAGAAT1360.11097601775616284No Hit
ACTCCGGGGGTTTGCCTGTGTGGATGGCATGGGGAGATTGGATTTGTGGAAT1360.11097601775616284No Hit
TCGAAAGGGATGGTCCTCCGATATTTACATTAAGAAGAGTTAAATTTATTTT1360.11097601775616284No Hit
ACGGATGGGATGCCCGGCCCATAAGTACCGTTTTTGAGGTTCAGTCTTAAAC1360.11097601775616284No Hit
TCTTTGGGGTCTTTTTGCTGGTAGATTATACAAGGGACAACTTTAGTGATGA1360.11097601775616284No Hit
GATCAGGGGGATTTAAAAAAATATTTCATCATCTGCTATATGCCATTATAAC1350.1101600176256028No Hit
GCACTAGGGAAGGTGATCTTTTATTTACCTGTACAGGAGTGTAAACTTTTTT1350.1101600176256028No Hit
GCACTCGGGGAGCCCAGGTGTTTCCGGAAGTCCCACGGCCTTGGGGCCACAG1350.1101600176256028No Hit
TTAAAAGGGATAATATATTTTGTGGGAGAAAATCTTTTCAGCTTTTAATTTC1340.1093440174950428No Hit
AAGACCGGGCATCTCTCTTTACTGCCAGTGATTACAAAGTGGCTTCTGCTGA1340.1093440174950428No Hit
TAATCAGGGAGAATGACTTTCAGGTGTTTTCAACTGAAAGTATATATCCAGA1340.1093440174950428No Hit
GGACATGGGTACATTTTTGAGCATTTGTCTATATTTGGTGAGAGAGAATAGA1340.1093440174950428No Hit
AGCTTTGGGAAATAACCTTGGACCTATGGGTCCTGTCCATGATAAAAGGCAG1340.1093440174950428No Hit
CCGTGCAGGGATATTTTGATGTTACTTTTCTCTTGTCACTTTTGAAGGGTGG1330.10852801736448278No Hit
ACTTTTAGGGGAAATCCTGAGCTGCAAGGTTCTGCCTCACCTGTCTCTCCAC1330.10852801736448278No Hit
GATACGGGGCGATCTAAACTCGGGATGACAGAGGTTACTCTAGCCTGCTTAA1330.10852801736448278No Hit
GCAACTGGGATTTACAAATATCTTGCCTTTAATCTTCATAGCATGAATTGTA1330.10852801736448278No Hit
AGTTAAGGGGGGTGTGTGCGCTTGGCGTGCAGGGTGGACGCGTGGGGTTCCG1330.10852801736448278No Hit
CTCAATGGGGAGAAAGTAGAAGGAGCTCCAAAGGCAGTGAGCGTCTTTCCTT1330.10852801736448278No Hit
TTGAGGGAGACTGGAAAAACTCATGTCCTAATCCTTTTTGTACTGTTGAAAC1320.10771201723392275No Hit
TGAGCCAGGGATTCCCCCACACAGAAGCAACCATTGTATCTAAATTGTGGTT1320.10771201723392275No Hit
TACAGAGGGTGGTTTGAGTGACTTGGTAGAAAATATAAGAAGGACAGATTTG1310.10689601710336274No Hit
CCGTTTGGGTAGAGAAATAAAAAACAAAAACAAATCCCCATCTATACAATTA1310.10689601710336274No Hit
AGAAGTGGGATCATTCCAGTTCCTCAATTTTTTGTCAAACCATTTTAGTTAT1310.10689601710336274No Hit
ATTGTAGGGACCACCACCCCTCTGGATGAAGCCTGCTGGGAGCGTGAATGAC1310.10689601710336274No Hit
GCTAACCGGGAAATCAGTGTATCTGACTACAGCCCCAGGGAGATTCTGTAAG1310.10689601710336274No Hit
ATGTGTGGGTCTTTGTAACTTTTGGGCTTAATTTTCATTCTTAAAAAATCTG1310.10689601710336274No Hit
AAACTAGGGATGTACCATGTGTGTGTCTCTATGTATTCTGGGGTCTTGAAAC1310.10689601710336274No Hit
AGTTATGGGAAGAGGCATCTAATATGAATTCTGCATTCACTCTACAATTTCA1310.10689601710336274No Hit
AACGAGGGATGTGCTGTGTTTTATGGATTATGTGTTTTATGTGCTGAATTTA1310.10689601710336274No Hit
AAGAAAGGGATCAGAATCATCAAACCCAGAAGGGTTAAAGAAAAAGAAAAGG1310.10689601710336274No Hit
GACAGTGGGAAAAGCTTATTGATAATTTTTTATCAATAACCATCAAGGATGA1310.10689601710336274No Hit
AATTAAGGGAGGGAGTTAATAATAAAGCTAATACATTCTGCTTATAATGTGC1300.10608001697280271No Hit
GCATGAGGGAAGGATGATGGCACCTTTTACCGGTTTCAAACCCCCTATTTTT1300.10608001697280271No Hit
CATCTGGGGAATCTTGAATGTAACCATTGAAAGGAAAATAGTATACAATTTC1300.10608001697280271No Hit
GGTGCAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1290.1052640168422427No Hit
ATTTATGGGATGTCTGGTTTTCATGTGGATAACATTTGGCAATCAAATATTG1290.1052640168422427No Hit
GTCAAGGGGGAACTTCATTTTTGTATCCTAAGAATTGTTTAATCTACCTTTA1290.1052640168422427No Hit
GTGTGAGGGGTGGCCCGGGCGTCCGCTCCTCAGCGGATGTGGCAGCCCCGAG1280.10444801671168266No Hit
TCGCAAGGGGAAGGGCAGTGTTAGGATTGCAGAATGGAAGGTCAACCCCGTG1280.10444801671168266No Hit
GATCGCGGGAACAGCAGATCTCTCTACAGAAACCCTACAAGCCAGAAGAGAA1280.10444801671168266No Hit
GATTTAGGGGTCAAAACTGGGTCTCTAAACTTTTCTTTTATCCTTTGTCTTA1280.10444801671168266No Hit
AAAGTTGGGGTTGATAAGAAACCTATATTGCTAGGTAGAATTTGTACTTGTT1280.10444801671168266No Hit
GTAGCTGGGATTGAGCTTATATTATATACCACTTTATATGCCTACCTAGTTA1280.10444801671168266No Hit
ATCCTTGGGACTGTTACATTTAATTCTCCAGAATTATTCTCCTGCTGTTGAC1280.10444801671168266No Hit
TCTGCAGGGAATACTGGCAACCAAGATTCTGTTAGGATTTCTGTGCATATAG1280.10444801671168266No Hit
ATGTCGGGGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAA1270.10363201658112266No Hit
ATAAGAGGGATACTTGATTGAACTGTAATTGTTTATTTCAGTTGTAGTTAGA1270.10363201658112266No Hit
TTTCAGGGGTATATACGTAAATCTATATCCAAATCTAAATGTCCATATCCAG1270.10363201658112266No Hit
ATTAAAGGGACTGATAAAAGAATTCAGCAAGGTTTCAAGATACAAAATTAAT1270.10363201658112266No Hit
TTTGCTAAGGGAGGCTTCTCGGTTACTTGCCTTAGTAAAAGAAGTATCTGGA1270.10363201658112266No Hit
GAACAGGGATCTTTGGAAACCTTCTCAAGAGCCTGATTGGGAAGAAGGAGAT1270.10363201658112266No Hit
CATAAAGGGGGGTGGGGGTGGTAGGAGCAGGCACACTCGTTGTAACTACCAA1260.10281601645056263No Hit
TTGCTCGGGACTTTATGAGGAAAATAAATATTTATGCCACCATATTTTTTCC1260.10281601645056263No Hit
ACTGCGGGAGGATATAGTCTCTGCATTCAAGTAGTGTGTGTATATTAAAATA1260.10281601645056263No Hit
TTGCATGGGGTCCTTTCCTACCTGGACATGTCCCATTAAAAAGTGGAAGATT1260.10281601645056263No Hit
TAGTGCGGGCTTCTAGAAAAGATTCACAATTTTAGATGCCATTAAATGCATT1260.10281601645056263No Hit
AGGTTAGGGACTAATTTTACTTCTAAGTCATTCATAAACCTTGTCTATGAAA1250.1020000163200026No Hit
CTGCCAGGGAAAGTGTCCAGCGGAATAGGGGCTGCAGCTGAAGTCCTGGTCA1250.1020000163200026No Hit
TTTTGAGGGGACATTTGTGGTGGGCGTTCTCATTGTGGTCCTGACCATCTGT1250.1020000163200026No Hit
TATCCAGGGCCCAGAAACTACTTTTAAACATACTGCACATTCAATTCTGTTT1250.1020000163200026No Hit
CTCATGGGTCCTGGAGTTATACAACTTGGCTTGTTTACCTCCACATGCTGAT1250.1020000163200026No Hit
CATTGCGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACT1250.1020000163200026No Hit
CTTATAGGGATTTTCTGAAGGCAAAAATGCATGTTTGTTAGAATGGAAACAG1240.1011840161894426No Hit
GACGCAGGGGTGGGGGACACGCAGATGAGTGAATTGGAATGTCCAGCAGAGT1240.1011840161894426No Hit
CTGAATGGGAGAAAAAACCTGTGGCAAAAACGTTTCTTTCTTATTTTTTTTC1240.1011840161894426No Hit
CGATCAGGGATTTCCTGAAATAACAAGTTTAGTGCTTTAACTATGGCTACAT1230.10036801605888257No Hit
AAACATGGGTCTCTGCGCGGGGCTCTCATTATGCCTGCCCATAGTAGTCTCC1230.10036801605888257No Hit
TTGCAAGGGATAGGAAATAGAGTTGTTTTTCAAATTTCCTTTTCTATTTTAC1230.10036801605888257No Hit
CTTACTGGGATGGTGATTTTAATGTTTAATAAACTATAATGTTGATTAATTT1230.10036801605888257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)