FastQCFastQC Report
Sat 31 Dec 2022
SRR5848238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122488
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGC6550.5347462608582065No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGCCGTCT3580.29227352883547775No Hit
TTCATTGGGAGAGAGCGAGTGAGCAAGCGAGCAGAAAAGAGGTGGAGAGGGG2040.1665469270459147No Hit
GTAGGCGGGGGTGTATGGATTTTAAGAAAGGAATCTAGCCAATGAGGTCCAA2030.16573052054078766No Hit
CATGTCGGGATGTGAGTTTTGACAAGCAGAGGTTTAAGTTTGGACAAAGTTC2010.1640977075305336No Hit
TCTAGCGGGTCCAAGAAAACAAAAAACAAGAAAGATACATCTATTATAAATA1920.15675004898439032No Hit
GACGTTGGGGTCCATTTACATTTATTGATTTCATAAATGTGTGTGGTCCTAT1810.14776957742799296No Hit
ACCACAGGGATTAGTTAAAATGGAAGGTGTTAGTTTAACAAACTGAGAGTCA1780.14532035791261186No Hit
GCACAGGGGATGATCGTGAAGTCTTTTACGTATTGAAAATTCGTTTGTTTCG1770.14450395140748482No Hit
GCTGCCGCCATCGTGCCAGCCCCTCGGGTCTCCGTGAGGCCGGGTGACGCTC1760.1436875449023578No Hit
TTGGTCCGGGGGTCCGCGGCGGGGAGTTGTTGCTGCCGCGATGCTGGTGGCG1750.14287113839723076No Hit
AACATAGGGACTGCAAGGATCCTAACACACACTTAAAATCAAGAGCCAAGGA1730.1412383253869767No Hit
CTCACAGCGGGATTGACACCGGTGTATAAGTATAAATTTAAATGAACTAATT1710.13960551237672264No Hit
TCAACTGGGCATGGTTCAGACAACAGAAAGCTGAATATGCCCCTGTGGCAGC1670.13633988635621447No Hit
CAGACGGGGAAGCACACAGTGCGAAAACTCTTGCTGATACGCGATATTGATT1670.13633988635621447No Hit
ATTGTCGGGACCGCAACATCTGGAAAAATCTGCTTATCCTGGGCTTCACCAA1650.1347070733459604No Hit
CTGTAGGGGGACAGCTTAGGCACTCCTAAGAGGAAAGGTCTGTGGAGATGAC1650.1347070733459604No Hit
GGACCGAGTCAAGTCAAAGGAAGCAAAAGAAGGAAAAAGGAGAAGCCAAAGA1650.1347070733459604No Hit
ACTTAGGGGGTACGGGTAATACTGATAGCGATATGTTCAATGATTAGTAGCT1650.1347070733459604No Hit
ACTTTAGGGAAACAGGGTTGAAGACCAAATGTATATTTCACAGTATCGTACT1630.13307426033570635No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTTATACA1630.13307426033570635No Hit
AGACTAGGGCAGTTATCAATGCGATAGACAGTAGACGAATGCAGGATACAAA1630.13307426033570635No Hit
GATCAGGGGGATTTAAAAAAATATTTCATCATCTGCTATATGCCATTATAAC1620.1322578538305793No Hit
TTGCGCGGGGGGAAGATGGACATTGTGTATCTCCAATTGTGATATCCTAAGA1600.13062504082032525No Hit
ATGCCAGGGTGGTGGTACCAGGAGACGATAAAGTAGAAAGTTGGAGTCAAAC1590.12980863431519823No Hit
ATGCACGGGACAAGCTGTGCAAACAAAGCAAGCAACTGCATTTCTCTGTCTC1590.12980863431519823No Hit
TTCAAAGGATTGTCTGGATTTGTTCTGGTTTTCAGGTGCAATGATTGCTGAG1590.12980863431519823No Hit
ACACAAGGGAAGTAAGTTTATTTTTGAGGAATGTTGTTAAAAATGTCTTTAG1570.12817582130494415No Hit
AAGATGGGGATGTATAATAATTTAAGTGGTATTTCCGCTTCCTTACTCAGTT1570.12817582130494415No Hit
ACTACCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1560.12735941479981713No Hit
GACAACGGGTAACCTGGGAAGTGGAACCGGATATTTAAGTACAATGGTGGGC1560.12735941479981713No Hit
AGCAACGGGGTTGGAATATACAGTTTGTATTTGTCTGCTGTGAATCATAATC1560.12735941479981713No Hit
GAACCTGGGATTATAACCATAGAATAATCACTAAAAAGTACAGAGAAATATA1550.1265430082946901No Hit
GCTCATGGGTTAGTTGTACAATTGAAAATATTCCTTATTATAGAAGTAAAAA1550.1265430082946901No Hit
TTAATGGGGACTGAATTAGAAATGGAAATGCCTGGCACAGCCAGGAAGGAGC1540.12572660178956307No Hit
TCGCGACGGGGCTGCTGTAGGTTTCATTCTCAACATCATTTTGTCCTTTCCT1540.12572660178956307No Hit
TAGTGCGGGCTTCTAGAAAAGATTCACAATTTTAGATGCCATTAAATGCATT1540.12572660178956307No Hit
TGAAGCGGGATCCCTCTCTTACTTCTTGTATTTCAGTGTTAAATAGAATTGC1530.12491019528443603No Hit
ATGTGCGGGGATTTTTTTGGAGGGTTGTCCAGTGTTTCCTGAAAATGCTTTT1530.12491019528443603No Hit
ATCCTTGGGACTGTTACATTTAATTCTCCAGAATTATTCTCCTGCTGTTGAC1530.12491019528443603No Hit
GACAGTGGGAAAAGCTTATTGATAATTTTTTATCAATAACCATCAAGGATGA1530.12491019528443603No Hit
TTTCAGGGGTATATACGTAAATCTATATCCAAATCTAAATGTCCATATCCAG1520.124093788779309No Hit
GAGCACGGGACTGTCCCAGCTCACTGAAAGGTCATAATCCAGGATGAAACCA1520.124093788779309No Hit
GGTCTTGGGAACTCAGAAGAGAAAAAATGGAGCCAAGGATGCTAACTTGGAC1520.124093788779309No Hit
ATTTTAGGGATTTCTACTGTGGGAATTTAAGGGCTAATCTTCATCATTCCCT1510.12327738227418197No Hit
GTGTGAGGGGTGGCCCGGGCGTCCGCTCCTCAGCGGATGTGGCAGCCCCGAG1510.12327738227418197No Hit
TAATGAGGGATTTTTATAGGGACAGATGATCAATCTTTCTTTTAGGAAATTT1510.12327738227418197No Hit
AGAAGTGGGATCATTCCAGTTCCTCAATTTTTTGTCAAACCATTTTAGTTAT1500.12246097576905492No Hit
GCACTTGGGATCTAGGTATCTGCTATTTTCATCTTTTGGCTTGTTAAACAGT1500.12246097576905492No Hit
GTTTTAGGGAATTAAGGTAAAAAAAATTACATAAAACATAAGCACATCAAAT1500.12246097576905492No Hit
CTTGCGGGAACTTTGGTATTTTTTTCTGAGATTTATAAGGTAACAGATTATT1500.12246097576905492No Hit
AGCTACGGGATATATGTGTGACTTCATATATATGTGTGACATACATATTACA1500.12246097576905492No Hit
TAGCTTGGGATTATCCATACATGTCAGCATTCCCTGGCGGAGTATGATGTAA1490.12164456926392789No Hit
GATTCGGGGATTTTGTCTATTTTTCTGTTTTATGTGAAAACCTTCATTTATA1490.12164456926392789No Hit
ACGGATGGGATGCCCGGCCCATAAGTACCGTTTTTGAGGTTCAGTCTTAAAC1480.12082816275880086No Hit
AACTGAGGGTCTTCCGATCTGCCTATGGGGGAAGGAAAGAGAGAGAGAAAGA1480.12082816275880086No Hit
CACAGGGGGGTTTTATAAGGGGCATTCCTCTTTGCTTGGCTCTCATTCTTCT1480.12082816275880086No Hit
CTTGTCGGGATCTGCTGTTAATTACACATGCTGTTATGGGTTTTAACCATGA1470.12001175625367383No Hit
TCAACAGGGAGTGCCAGTTATGAGAATCTGGTGTCTTCAGTATGTAGAATAA1470.12001175625367383No Hit
CAGCTTGGGTGTTTTGACTGTGGCTCATCACATGAGGTCAGGGGTAGCATTT1460.11919534974854679No Hit
TAATGCGGGCCAACAACTCGTCCATCGAGCTGGAGAAGCTGGCCAAGAAGCA1460.11919534974854679No Hit
CAATAAGGGATGTCCACATCTTGTCCCACTGGAAGGTCTTCAGGGCCAATAA1460.11919534974854679No Hit
GTAGACGGGTAATAGGTATGTGTATTAGTCTTCCAGTGCTGCACAGCAATGT1460.11919534974854679No Hit
CCGTGCAGGGATATTTTGATGTTACTTTTCTCTTGTCACTTTTGAAGGGTGG1450.11837894324341976No Hit
GTAGCTGGGATTGAGCTTATATTATATACCACTTTATATGCCTACCTAGTTA1450.11837894324341976No Hit
CCTCATGGGTCTTCAAGCAATGGGCCTTGTACCAAAGGACCTCATGGCTAAT1440.11756253673829273No Hit
ACTTTGGGGACTAATTTTCAAACTAAAGCTAATTATAACTGCTAAACTTGAA1440.11756253673829273No Hit
GCAGTTGGGAGCTCTGTTATTAAAAAGGTACTGATTATGAAAGAGAGATGGG1440.11756253673829273No Hit
TGCTGCTGGGATGAAACGTTTCCTGTATTCTAATCTATTTTGAAACATTTTG1440.11756253673829273No Hit
GCGCAAGGGATTTGTTTTAATTTAAACAAAACTATAATCAACTTGCTTATCT1440.11756253673829273No Hit
TCATAAGGGACCATCTCAGGATAAGGAAGACGAAGGGATTTAGTCACCTTAA1440.11756253673829273No Hit
AGTTTAGGGGTGTCTGTCTTGTGATGAAGCACATTATAATACTTTAGACTTG1430.1167461302331657No Hit
AAACCTGGGCACCTGCTGATTTTAGAGTCCCAGGGCCTTGAGTAAATATAGG1420.11592972372803867No Hit
TACTACGGCCACTACATAATAGGGCTCAGCCCAGGCAGCCAGCTTTGGGCTG1420.11592972372803867No Hit
GCTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCAAAGCTGCCTTTTT1420.11592972372803867No Hit
TTAGCAGGGGTGGGAATTCAAAATGAGATTTGTGTGGGGACATGGCCAAACA1420.11592972372803867No Hit
ATATATGGGATGAGCTGATGTCAATGCTATGGACTGCACAAAAGTCTCAATG1410.11511331722291163No Hit
GTCTGAGGGTATTTACAGTTAATGATCAATTCACATCCAAACTACACAGAGT1410.11511331722291163No Hit
GCGAACGGGATTACTGGAAATGTTCCTGTACATGTTGGCAGCAGATAAAGAT1400.1142969107177846No Hit
TGGCGTGGGATACTTCTGCTCTGACCTATGATGCCGTTCAAGTGATGACTGA1400.1142969107177846No Hit
AGATATGGGAAAGATTAAAAGGAAAGGAAATTAATCGGAATTTTGACTATAA1400.1142969107177846No Hit
AGATAGGGGCCACCAAATCTGACTTTTACATTTGGTCTCAGAATTTAGGTTC1400.1142969107177846No Hit
GCTAACCGGGAAATCAGTGTATCTGACTACAGCCCCAGGGAGATTCTGTAAG1400.1142969107177846No Hit
CTTATAGGGATTTTCTGAAGGCAAAAATGCATGTTTGTTAGAATGGAAACAG1390.11348050421265757No Hit
TGACTGGGGAGGAGCATTGTCTCAAATACTGAATTGGGTCACTGGGAAGAAT1390.11348050421265757No Hit
TCTATTGGGGTTGAAATCTCTGAAAAATTTTATTGAAAAAAAATCCATATAT1390.11348050421265757No Hit
TTTGTGGGGTATAGAAAATTTTTTGAAACAAAGTTATGTCAGATATTTTCAG1390.11348050421265757No Hit
TTCCATGGGGTATTTTAATTTTGTATCTGATACATAAGCATGTTTCCTGACT1390.11348050421265757No Hit
AGCAATGGGGAAAACCAAGTGTGGGGAGCCGTCGCGCCCCGCCGCCTGCCGA1380.11266409770753054No Hit
AGCTCCGGGACAAAAACAAATGAAAGAAGAGGATTTTGTTTTATCACATATA1380.11266409770753054No Hit
GTAAATGGGTACGTAGAAGGGACTGCAGTTGTGATGGGTTTTGAAGATCCCA1370.11184769120240351No Hit
CGATCAGGGATTTCCTGAAATAACAAGTTTAGTGCTTTAACTATGGCTACAT1370.11184769120240351No Hit
AGCTTTGGGAAATAACCTTGGACCTATGGGTCCTGTCCATGATAAAAGGCAG1370.11184769120240351No Hit
GTGCAGGGGGGCGGCCGAGTGCGGGACTGGGGCTCCTGGCTGTGGGTGTGGT1360.11103128469727647No Hit
GAGTCTGGGAACCAAGAAGTAGTACTTGATTGGTCATTTGAAAACACTGCAA1360.11103128469727647No Hit
TGACCGTGGGCTTCCCAGCACCTTGTGCCCTTGCCAGTGAACGCAGGGTTTG1350.11021487819214944No Hit
ATAAGAGGGATACTTGATTGAACTGTAATTGTTTATTTCAGTTGTAGTTAGA1350.11021487819214944No Hit
TCTCTGGGATTCTGGCAGCTCCTATACAAGTCCCCCATCAGATGCTGGTATT1350.11021487819214944No Hit
AACAACCCCCTAGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTT1340.10939847168702241No Hit
GGCACAGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCA1340.10939847168702241No Hit
TGAATAGGGGAGGCGGCGAAGATGGCGGAGAACAGCGGTCGCGCCGGCAAGA1330.10858206518189538No Hit
GATAGGGCTTGGTCCTTGGAAGACCTATATTCTCTGTAGTTACTGAAAACCG1330.10858206518189538No Hit
TATGACGGGAACTGGAATTAGAGTCTGGGTCTCCCAACCACTGATTCTGCTA1330.10858206518189538No Hit
TATCGGGAACTGGGTTAAGCTGGTGATGATGATATAATTCCTCTTTTGTAAA1330.10858206518189538No Hit
CTATTAGGGGAAATATCTCCTGGGTGAGAATTGCTCTGCAACGCATCCCGAT1320.10776565867676835No Hit
GGTAAAGGGGAGGGGCCGCCAGGATGCCGGCTTGGGAGTTGGGGGTGGCAGG1320.10776565867676835No Hit
AAACTAGGGATGTACCATGTGTGTGTCTCTATGTATTCTGGGGTCTTGAAAC1320.10776565867676835No Hit
GCACTCGGGGAGCCCAGGTGTTTCCGGAAGTCCCACGGCCTTGGGGCCACAG1320.10776565867676835No Hit
TGGTCTGGGGGCATGTCTTCCTTCTGGGAGGGTGTGCAGGCCTTTAAACTGA1310.1069492521716413No Hit
TTGCTCGGGACTTTATGAGGAAAATAAATATTTATGCCACCATATTTTTTCC1310.1069492521716413No Hit
GTGAGGTGGGAGCTACAAATGCCTATTTATTCCCTAATGTACCTGAACACTA1310.1069492521716413No Hit
TTTCACGGGAGGAAATCATAGATGCTGTAGACCATGTTGAGTTGGATTGTGT1300.10613284566651426No Hit
AATTAAGGGAGGGAGTTAATAATAAAGCTAATACATTCTGCTTATAATGTGC1300.10613284566651426No Hit
TGGCATGGGAAAGTGAAAAAGAAATGACTATATACAATCAATGCTATTTATT1300.10613284566651426No Hit
TCTGCTGGGAATCCCAGCGATGGACTGGATTCTGAAGGCCACTTCCCACCAT1300.10613284566651426No Hit
CCCACTAGATGCACCCACTACTTCTGCTGATACTCCATTAGCTAATGCTTGC1300.10613284566651426No Hit
CTCAGGGGGGTAAGTCATATTAAAATTCCTTGTTTTACAGTTGAGGAAATGG1300.10613284566651426No Hit
GTCAAGGGGGAACTTCATTTTTGTATCCTAAGAATTGTTTAATCTACCTTTA1300.10613284566651426No Hit
GAACAGGGATCTTTGGAAACCTTCTCAAGAGCCTGATTGGGAAGAAGGAGAT1300.10613284566651426No Hit
ATAACTGGGAGGCTAAATTGTGGAAGCTAAGAACATAAAGTACATTGATTTC1290.10531643916138723No Hit
TAAGCTGGGAGTTGTTTGTTGATTATAAAATTGAACCAAGTATAGGGAATTC1290.10531643916138723No Hit
AGGTTAGGGACTAATTTTACTTCTAAGTCATTCATAAACCTTGTCTATGAAA1290.10531643916138723No Hit
TCGAAAGGGATGGTCCTCCGATATTTACATTAAGAAGAGTTAAATTTATTTT1290.10531643916138723No Hit
ATTGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1290.10531643916138723No Hit
TGAGCCAGGGATTCCCCCACACAGAAGCAACCATTGTATCTAAATTGTGGTT1290.10531643916138723No Hit
ATGGCTGGGACTACCTTGTTCCTGTAAATATTAGTGCAATGAACCTTCTTGT1290.10531643916138723No Hit
CCGTTTGGGTAGAGAAATAAAAAACAAAAACAAATCCCCATCTATACAATTA1280.10450003265626019No Hit
TATAGGGGGTTAAATCCCTTTGGGTTTTTCCCCAAGCCCTGCAATACTAATA1280.10450003265626019No Hit
ACTTGAGGGGATTTGCAATTAGATGTTTCCATTCTTGAAAGAAAAAAGCTGC1280.10450003265626019No Hit
AGCAGGGAATTGCACACAAGTAATACTACTGGGTAACCAGAATCAGGTGTGA1280.10450003265626019No Hit
AGCATGGGGGTTCTAGCGCAATAAAATGTATTTACTGTTTTCCAAATGATAT1280.10450003265626019No Hit
ACTGCGGGAGGATATAGTCTCTGCATTCAAGTAGTGTGTGTATATTAAAATA1280.10450003265626019No Hit
GCGAATGGGCTTAAAGTTCCGGTTTTCAAACTTAGGCAGGTCATATTCTATC1270.10368362615113316No Hit
ATTATTGGGATAGGAAGTCTCTTTAATGCAATGATTTGTTTTATATTTGGAC1270.10368362615113316No Hit
TTGTGGGGGGCTTGGACGTGACCCTGGCACCCAGGCAGGTGGATCCCAGCTG1270.10368362615113316No Hit
AGTTAAGGGGGGTGTGTGCGCTTGGCGTGCAGGGTGGACGCGTGGGGTTCCG1270.10368362615113316No Hit
CTCAATGGGGAGAAAGTAGAAGGAGCTCCAAAGGCAGTGAGCGTCTTTCCTT1270.10368362615113316No Hit
ACTCCGGGGGTTTGCCTGTGTGGATGGCATGGGGAGATTGGATTTGTGGAAT1260.10286721964600613No Hit
TCGATAGGGAAGCCAACAAACCCTCTGTAGCTATAGAATGAGTGCAGGTTTC1260.10286721964600613No Hit
ATGTCGGGGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAA1260.10286721964600613No Hit
AAGACCGGGCATCTCTCTTTACTGCCAGTGATTACAAAGTGGCTTCTGCTGA1260.10286721964600613No Hit
TCTTTGGGGTCTTTTTGCTGGTAGATTATACAAGGGACAACTTTAGTGATGA1260.10286721964600613No Hit
CGTAAAGGGATGATTTTGTTACAATCTCAAAGTATGTGACATAGTGTTATTT1250.1020508131408791No Hit
ATTGTGGGATTAACTTGAAATGTCAAAGTTGAGGTGAATTCTTGCTTTTCAA1250.1020508131408791No Hit
TGGCCTGGGCACAAGTTCTGGTTCTTCAGTGCTGCTTACTATTTTGGTAACT1250.1020508131408791No Hit
AAATACGGGAAGAGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAGTTGAAAG1250.1020508131408791No Hit
CATTCAAGGGAGAGGAACTAACGTCCAACGACAAGACGAGGATTCTCAACGT1240.10123440663575207No Hit
AGATAGGGACATCACACCGTATCAGCCTTCAAAATATTGACAAATTATATTA1240.10123440663575207No Hit
GATCGCGGGAACAGCAGATCTCTCTACAGAAACCCTACAAGCCAGAAGAGAA1240.10123440663575207No Hit
GAACTTGGGTCCGAAGAGTGATGCCCACCCAAGAAGTTCAGGAGCTGCCGAT1240.10123440663575207No Hit
TTTTTAGGGATAATTGCAGGATGATCATTGATTACTTGAAAGCCACTGCTTA1240.10123440663575207No Hit
ACCAGGAGACGATAAAGTAGAAAGTTGGAGTCAAACACTGGATGCAGAAATT1240.10123440663575207No Hit
TATATAGGGAATCATGTAAATCCTGGCAGGTGACCAAGTATGTCTCTAGAGG1240.10123440663575207No Hit
TGTAATGGGGTGACCCTGATTTTGATCCTAGTTAAAAAACATTAGTGGAACC1230.10041800013062503No Hit
GTTGCCGGGATAAGTGACAAGTTTGGGTGCTTGTGGCACGTATGTATGAAGC1230.10041800013062503No Hit
TAACAGGGAGAGGAGATCAAGGAGAAAAATAAAGCTGGAGATATAAAAACAG1230.10041800013062503No Hit
AACTACGGGACTCCAGTGACTAATATTACAAGCAGCATCATGCAGAAAGTGT1230.10041800013062503No Hit
TAATCAGGGAGAATGACTTTCAGGTGTTTTCAACTGAAAGTATATATCCAGA1230.10041800013062503No Hit
TTGCATGGGGTCCTTTCCTACCTGGACATGTCCCATTAAAAAGTGGAAGATT1230.10041800013062503No Hit
GCAACTGGGATTTACAAATATCTTGCCTTTAATCTTCATAGCATGAATTGTA1230.10041800013062503No Hit
GGACATGGGTACATTTTTGAGCATTTGTCTATATTTGGTGAGAGAGAATAGA1230.10041800013062503No Hit
ATGCATGGGACTGCCAACTTCAAGTGATTTAGATATCTATCTATCTAGATTT1230.10041800013062503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)