FastQCFastQC Report
Sat 31 Dec 2022
SRR5848219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71032
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGCCGTCT6920.9742088072981192No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGC6780.9544993805608739No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTTATACA1630.22947403986935463No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTT1380.19427863498141681No Hit
TGCATAGGAAATTCAGCATTATACTAGAGCTCTAAGTCAATGCAATAAAGTA1180.16612231107106656No Hit
CGAACTGGGAGCCTATGGAAGTAATATACAAATACCGGAAATTTTAGAAAAA1120.15767541389796147No Hit
AAACCGGGTAATGTATCAGAAACTGTACTTACAGATTATCAAGTAGGGAACA1070.1506363329203739No Hit
TTCATAGGGATTCTGGTTTTCAACCAAGTATCTATGCTTTTCTGCTCAAACT1070.1506363329203739No Hit
AGACCAGGGAGAGCAGTGCTTCTCCATTTGTTTTGCAGAGAAATGTTTTTCA1070.1506363329203739No Hit
TTTGTTGGGGGTGCCCAGTACTTCCTGGACAAGATCGACGTGATCAAGCAGG1050.14782070052933888No Hit
ATCTGTGGGAATGAGTAAAGCAGAAAAACTTTTAACCAGCAACATTTCAAGT1050.14782070052933888No Hit
ACATTTGGGCCGTCTGAGGAGCAGTGAGGTCTTCAAGTGGCTCATCCCTGAC1040.14641288433382138No Hit
AGACTAGGGCATTCTGAACTCTTCTGCTTTCTCCTGCAAATTAAGTTATTGT1020.14359725194278636No Hit
ATCTTAGGGGAAGACAACCTTGATTTCAGTGTGAATTCAGAGGTGTCTGTGT1020.14359725194278636No Hit
ACTTTCGGGAGATGTTCAAATTTCAGATCTTACCATCATCCAACTTAAACTG1010.14218943574726883No Hit
TTACTCGGGGCTGATATGAATACTTAGTAATAATACAAAAGTTCCTGCTAAA1010.14218943574726883No Hit
TGTATTGGGGACCAAATGCAATTTGGAATTCAGAATTCTTCATATTTTAGAA990.1393738033562338No Hit
AGCACAGGATCCATGAAATGACTTGGGTGATAATCCAGTAATTATCAATAGT990.1393738033562338No Hit
GCGTTTGGGAGGCTTTCTTTCCCAGTGGGTCATTTTTGTCCTAGTTCAGTGT980.1379659871607163No Hit
TAATCCGGGACTATAACCAAAAAAATGTTATGTTTGATTTGAATATGACAGA970.1365581709651988No Hit
CTTTAGGGGAAGAGGAGTGGGGAGTAACTGGAGGCAGTCCTAAAGGTGGAAT960.1351503547696813No Hit
ATTAACGGGATTTTAATTCCATTACTTTCAGTCTGGAAAACTAAGCTAGTCA960.1351503547696813No Hit
GGCAATGGGATGCTAAACTAGTTATCACTTGTCTGTCTGTCTCTGGCCACTT960.1351503547696813No Hit
AATACTGGGACCAGAAAGAGAGAAACACCTAAGAAGTTAAATAACTGTCAGT960.1351503547696813No Hit
TGCTACGGGTGAGGGTGAAATTAAGGGAAGGCAAAGTCCAGGCACCAGAGTG960.1351503547696813No Hit
GTATATGGGACCCTACAACCATCATGAATGCAGATATTCTAGCATATTGTCA950.13374253857416377No Hit
TGTTACGGGGAGGGGACCCTGCTCAAGCCCAACATGGTGACCCCGGGCCATG950.13374253857416377No Hit
GTGTGCGGGATTTGCTGGCTGTAGTGTTAGCTAAGCAATGACAAGAACTTTC940.13233472237864624No Hit
TTCACTGGGGAGCCCTACCCCTTCCAGTATATTGTATTTCTTTATCCACATT940.13233472237864624No Hit
TAAAGAGGGACCACCTTTACTGTACTGTTGGAGCAGATCTTTATTTTACAAG940.13233472237864624No Hit
TACCTGGGGAATCACACTCATATTTGTGTAACCAACTTAGAATATAACCAAA940.13233472237864624No Hit
GCACTAGGGGGGCTAGATTTGGGGATTCCGATGTCATTATTATCTCTGCAAT930.13092690618312874No Hit
ATCACTGGGAGTCCAGCCTTGTGTTCACCGGAGCTTAAGCGTTGCAGCAGGA920.12951908998761122No Hit
AAAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA920.12951908998761122No Hit
GCCTGGGGAGAAATTCAGCTGTCATTCAGTTACTCTTAATTCTTTATAAAAC920.12951908998761122No Hit
GGATACGGGCTTATGTAAGTATTATCTAATGGCATTTTCTTTATCTCTTGAG920.12951908998761122No Hit
CATTGGGAATTGTAAGATTGTACCTTTCAAGAAAACTCAACCTTATTTTTTC900.1267034575965762No Hit
TTCAACGAGGGGTTTTGAAAACGTGTGATAGGAAAGCAAATGTTGCAATCCT900.1267034575965762No Hit
GCCATAGGGAGTTGAAGTGTTTTTAAAAACACTTTGTTTTGTAATGTTTTGA900.1267034575965762No Hit
ATGAATGGGATATGACAAAAGTATATTTGAGTCAAATGTGGCTTTCTAAAAT900.1267034575965762No Hit
GCTTCAGGGTACCAAAAATATTTAACTTGAATCTGATCATGATGAAACAATC900.1267034575965762No Hit
CTTTGTGGGAATGAAAAATTTGTACAGTTTAAACAACAGCTGAAAGAACTAA890.12529564140105867No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGT890.12529564140105867No Hit
ATATGCGGGGATAAATTTACCTTGAAACAGCAGATGAGCAAAAAGAAAATAA890.12529564140105867No Hit
TTCATAGGGGCTTTCCAGATGCTAAGAAGCAGCAGTGGGGCTTTTGAATTTT890.12529564140105867No Hit
AAAGCAGGTCTAGCAGTGGGGGCGGAAGGCTGGGAGGGCTGCGCGGGCTGCG890.12529564140105867No Hit
TAAGCAGGGAATCTTTGAAGAGCTTAACTAAGTAGGTAATTGAGATCAACCA890.12529564140105867No Hit
TACTGCGGGGTCCTTTAACTTTTTAAGTTGTCTTCAGTGGAGAGTTTTTCTG880.12388782520554116No Hit
CGCTATGGGCAACTTGGGGGATAGGAAAGGTTCAATTTAGCATCCTGTTGAC880.12388782520554116No Hit
GGCTTGGGGGACATATAAATGGTAAATTATATTTAGGCTTATCCTGTTTTGA880.12388782520554116No Hit
TTCCAAGGGTACCCTTAGGAGTTGTCTCGTTGTTAGTTTACAGGTTCTCCCC880.12388782520554116No Hit
TAGAAGGGGACCCACACCTTTTTTTCGTAGGTGGGCTTTTCCTATCAGAGCT880.12388782520554116No Hit
ATTAGTGGGAACTAAGCAAAATTCACCTTAGTAGAATAATTTAAAAGCTCTT870.12248000901002366No Hit
GTAACGGGGAGGGAATCAGCAACTAAAAGCTTATATTACTTTATACTTAACA870.12248000901002366No Hit
ATGAATGGGCGATGCTATTGCTGATGAAGTTTGCCATCAATATGCAATGCAA870.12248000901002366No Hit
GTTTTTGGGAGCATCTCATCTTAGTGGACAACCCTGATTGCTAGTGAAGATC870.12248000901002366No Hit
GACATGGGGGATCTAAGAAATTTTAGTAGTGTAATTCTACTAAACGTATCTT870.12248000901002366No Hit
CGCACAGGGGTTTGCCATTTTGTTAAAGTACATTGCCCCTTAAATTTTGGAG870.12248000901002366No Hit
GCACACGGGAATTTAAAAAAACCTTCATAATCTGAAATAACAGTTCATTTGA860.12107219281450614No Hit
AATGGTGGGGTTTGAGCCGAACAACTCACCACACTTGGCATCACTAAAAGAA860.12107219281450614No Hit
GGTCATGGGAATATGACATTAGGAAATTTAAAACCGCAAGAAAGGAAGGATA860.12107219281450614No Hit
TGGCATGGGGAAGTCAGGGGTCAGAGACCTGGGTCTGTCACTTAGAAGTTGA860.12107219281450614No Hit
ATTCGGGAGGCTGAGGTGGGAGGATCATTTGAGCCCGGGAGGTTGAGGCTGC850.11966437661898863No Hit
TTTGCGGGGGGAGAGAGAGCCTCGGCTAGGTGTGGAAACAGGAAGAGAACTA850.11966437661898863No Hit
ACACAAAGGGACAATAGGTCTCCAATATGTATGGCATCAGACTTCTCAGTGG850.11966437661898863No Hit
ACGCATGGGACATGTGCAGGATGTGCAGGTTCGTTACATAGGTAAATGTTTT850.11966437661898863No Hit
ACCAAAGGGTAGTTGTACAATTGAAAATATTCCTTATTATAGAAGTAAAAAA840.11825656042347112No Hit
CACCACGGGAAGTAAGTTGTAGAGTAGATTCAATGTAGTCACTATAAAATTT840.11825656042347112No Hit
TTGAAAGCGGGATTTTTGGTGGAGACAAGGTTTCGCTGTGTTGGCCCGCCTG840.11825656042347112No Hit
GCCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGCGT840.11825656042347112No Hit
TGCAGTGGGCCCAACTCTATGTAAGTAGTCATTGTCCTGTAAGTAGTCATTG840.11825656042347112No Hit
GTATGAGGGGACTCTTATGATACTGATTTTCCTTTAATGTTAATATGCCAGA840.11825656042347112No Hit
TGCAATGGGATCCACCATGAACCAATGCTGTGAGCTGCTTAGTTTGCTTGTT840.11825656042347112No Hit
AAACAGGGGAGGGAAGACTGTTGCCGGATGACAAGGCTGTCACTATTGTTCT830.1168487442279536No Hit
GTGTTTGGGGGACTGTGGGAGTCAGACAGCCTGAGATTGTAGTGATTGTGGA830.1168487442279536No Hit
TATTCTGGGAGTTAAAAATGTGGATAGATACTGTGAAGCTGCTCGCTCAGAA830.1168487442279536No Hit
AATCTAGGGAAGCCAGGCTCAGAAAGACGGGTCATGCATGTTCTCACTCATA830.1168487442279536No Hit
ACCTAAGGGTAATCCAATTATCACACAAGTAAATGTAAAATGATGGCTGTGT830.1168487442279536No Hit
ACTAATCGGGAAGGATGATTAGACATCTCACTACTACTACTGACTATCACAC830.1168487442279536No Hit
TTTTGGGGGAAATGGAATTGGGCATGAAAAATGTGAACGGCACAAGGCAAAT830.1168487442279536No Hit
CAAAGTGGGAGGATCAAATATGGAAATGGCAATGGTAACTCTGCCTAGAACA830.1168487442279536No Hit
CATTGAGGGTTGGGTTCCCCATTAAATCGTTTACATACAAGAACACAGTTTT830.1168487442279536No Hit
ACCAGGGAGTTTGACAGGAGGAGAATAAGGCTGAAAGCCTCAAGTGAAGGAT830.1168487442279536No Hit
AAATTTGGGCAGCATCGCGGTTCTCCGGGGCTTGGCGTACCTCCGAGAGAAG830.1168487442279536No Hit
ACAAATGGACACATTCTATTCAACAGTATATGGAACTTTCTCAAAGATAGAC820.11544092803243608No Hit
CAACGACGGGTCTAAACTTGGGGGGAAAAGGGCATAAAGCTATATAGAAATA820.11544092803243608No Hit
TATTGCCGGGATTTTGAATAAGATACTATTCACGCACATGTCTGTATGTGGA810.11403311183691855No Hit
ATCTTCGGGATACCAATCTTAAAGCAAAAGCTTTCATATTTTCCCTATTCAT810.11403311183691855No Hit
GCAACTGGGACATAGACAAAAATAGTTTGACAAAACTGCAGGGAAGGAAGAG810.11403311183691855No Hit
GAGTCAGGGGGTCATAGGTTACTTTTATATATTATTGAATTTATAATAAACA810.11403311183691855No Hit
ATCACGGGGGCAGAGATCTTGAGTCATTAAGGTCTGGTGTCTCAGAAATCTT810.11403311183691855No Hit
AGATACGGGATTTTTTTAAATTGTATTAGTAATTTATGTTTAATACAAAAGG800.11262529564140106No Hit
TTGTGCAGGGACCAGAGAGGCAGGAGAGCCAGTCTTGCAGGCATGCTGCCAG800.11262529564140106No Hit
GTGGTCGGGACCTTGAAATTATTTGACCAAGAAGCTCAAAGAAAAGAAGTAC800.11262529564140106No Hit
TCAAAGAGGGCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCA790.11121747944588353No Hit
TTAGTGGGGAGTGGGGCAGGGGGCCTGCCCTGAGGCTCCCAACCATCCTCTC780.10980966325036604No Hit
TGCTACCGGGGCTTATCACTGATAAGAGCCCAATAAAACTATTTGACCATTG770.10840184705484851No Hit
CCCAACGGGAACAGAAAAGCTTTTGTATGTCTCTTTTTTTGTTTTTTTGTTT770.10840184705484851No Hit
TCAAAAGGGGAGATTGCTGCCATTTTTTGGTGCTTTTTGGACCTCGTCATAA770.10840184705484851No Hit
TATGGCGGGGAGACAGTGAACTAGCATTTGAATACACAAGTCCAAGTCTACT770.10840184705484851No Hit
CACCACCGGGGCGCATGATGAAGAGAGCACCGTGAATCTAGTCTTGACTGCC770.10840184705484851No Hit
TTTTAGGGCTGGCTCAGGGCGCTCTTCCGATCTGCTTTAGGGTGCGACTTCC770.10840184705484851No Hit
AGCTGGGGGTCAGGACTTGTTCATTAGGTTGGCAGCAGAGGGGCAGAAGGAA770.10840184705484851No Hit
GAAACTGGGTGCACATGTGAGGGATCTAGGTCGCGTGCTCCTTATGAGAATC760.10699403085933101No Hit
GCTAAAGGGACTTGCTATTTTAATCAACATCAAAGACCAGATGATGTGCTTC760.10699403085933101No Hit
GCAGCTGGGAACTACTGTAGACTATTGAAGAATGATCTCTTTGTGATTTAAG760.10699403085933101No Hit
CATACAGGGGCTCCTGGGCCCTTGTAGAGACTGAACCATGGAATGCCAAATG760.10699403085933101No Hit
ATCGGGGATCTCCCTGTGTGCCTAAATTTTCTCTTCTTATAAGGAAATTGGT760.10699403085933101No Hit
AATATAGGGACTTGGGAAAACTCTACCACCAAACTGTTTGCAAGGTTACACA760.10699403085933101No Hit
GTGCCAGGGATTCAGTCAAGACTAACCACATTTCCAGTGCTCAGTTACATTA760.10699403085933101No Hit
ATACGTGGGCTAAGATAAGTGTGTGGGTATGTAAAGTAGCACAGTCATTATG760.10699403085933101No Hit
TATCGTGGGCTAGCTGTGGGACTAATGTTTAAGTTGATTTATCAACAGTATT750.10558621466381349No Hit
TTGTCGGGATGTCTGTGGATGACAAAAGGCTATAGCCACTATTAAAGCTACA750.10558621466381349No Hit
AGCACTGGGATGACAAATTACCCTAGAATGGGTGGTTTAAACAATGAATGTT750.10558621466381349No Hit
TCATCAGGGGGGCTCTGCCTGGGTTGGAACTAGGGGGAGGGATAAGCTCCCT750.10558621466381349No Hit
CCAAGTGGGATAGGCTGAAGGTATTTAAGCACAACCTCTGCCCAAATCAGTA750.10558621466381349No Hit
ATTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA750.10558621466381349No Hit
AGTGAGGGATCCTCAGTGCTATTAATATTTTGGGCCACCTAATTCTTTGTTG750.10558621466381349No Hit
TCTGGGGGCTGTCCAGTTTTATTTTTTTTATGTTGTTATCCTTGGATGTACA750.10558621466381349No Hit
GACTGTGGGAGTCCAAATTATTTTTGATTTGTAAATTATTAGGAAAGTTTTT750.10558621466381349No Hit
TGCCATCGGGCTACTTTTCTCAGTGTTATATTTGGTTATGAATGCATTTACC750.10558621466381349No Hit
GAATTAGGGAAGCTTTATACAAATGGATATCGTGCTGAAGTGGTATTTACCT740.10417839846829599No Hit
TTTCAAGGGATTTGGAGGTGGGGCCTTTGGGAGGTGACTAATCATGAGAGTG740.10417839846829599No Hit
CTTACAGGGGCAGGGTCAAAAGACAGTTACTAGCAATGGGGAATGCTTGTCA740.10417839846829599No Hit
GGACCTTAAAACAATACTGGTGTCTACAGCTATGGGCTGCTTCACATTCCTG740.10417839846829599No Hit
GTAATTGGGAGGCACTGGTTAACCAAGTTGTACACATTGTACCACATTCAGC740.10417839846829599No Hit
GGTTATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC740.10417839846829599No Hit
AGACACGGGTACAGGATTTTGATGATCAGACTCCAAAGTCTAATTAACAGTT740.10417839846829599No Hit
GGCGCAGGGATAGGTGCCCAGGTCATCTATAAAAACGATCCTTGGGCTGTGT740.10417839846829599No Hit
AAATATGGGAAATGGAACATGTCTAAAACCCAACTCCTTTTCTTTCTTTAAA730.10277058227277847No Hit
CTATCGGGGATTTTTGATAAAAATGAATCCACTGACATCGGGGAGGAGCAAG730.10277058227277847No Hit
GAAACTGGGATCACATTGCTTATAAAACTTTTAAAATCCTTGTTTGTATAAA730.10277058227277847No Hit
ATGTAAGGGTGTGGGCTGGGGAGAAACAGTAGTCAAAGCTTTCTGAATTGAG730.10277058227277847No Hit
CTAAGCGGGCGATCTGCTAAGGGGCACCACAGTATCTCCAATGCCTAGGCAC730.10277058227277847No Hit
GTTATCGGGCAAATATTTTTAAAGAATTTTTATGTTTTCCAAAATTAGGATT730.10277058227277847No Hit
AACCTCGGGATCCCTCACCTGCCAAACATAGCCCCGGATTCAAAAATAATTA720.10136276607726095No Hit
GATAAAGGGGTAAGAAGAACATTTCTTTGGAAATAGGACTATTGAAAAGCTG720.10136276607726095No Hit
GTTTCTGGGATGATGCTCAAAGGAAATGCTCATTTGAGCATTTTGGATTTCA720.10136276607726095No Hit
GGCACTGGGGACAATCAACGAGGACATGGGGCTGGAGGAGCCTCTATTCTTA720.10136276607726095No Hit
TATAGCGGGATGATAAAAATGACTTGCTACTTCAAATAGGGACACTAGAGGA720.10136276607726095No Hit
CAGCGTGGGTGTGCTAGATAGGAAAATTGTCTGTTGTGATTTTAAGTGGGAA720.10136276607726095No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)